한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

LC-MS/MS를 이용한 S.D. Rat 혈장 중 Arctiin 분석법 개발

송병정,채정우,백현문,권광일 충남대학교 약학대학 의약품개발연구소 2014 藥學論文集 Vol.29 No.-

KIOM-MA128 is a novel oriental herbal medicine which is for atopic dermatitis and asthma. The purpose of this study was to develop on analytical method of arctiin in rat plasma after oral administration of KIOM-MA128. Analyte was separated on a Atlantis dC18 reverse phase column, using gradient mobile phase (A:B = acetonitrile: 0.1% formic acid in water) at a flow rate of 0.3 mL/min. Detection was performed by electrospray positive ionization mass spectrometry using multiple reaction monitoring of the transitions of arctiin at m/z 552.4 → 372.8 and internal standard (carbamazepine) at m/z 237.0 → 194.5. The limit of quantification was 1 ng/mL for arctiin. The precisions were lower than 15% and the accuracy was between – 12.21 and 3.2%. The maximum concentration found in plasma samples was 4.1 ng/mL. The present method was successfully developed for detecting arctiin in plasma and this results would be utilized to the further study.

공무원 다문화역량 교육프로그램 개발연구를 위한 요구 분석

이병준,박응희,한현우 한국문화교육학회 2010 문화예술교육연구 Vol.5 No.1

노동의 세계화와 국제결혼, 다양한 종교의 유입 등 변화하고 글로벌화하는 사회환경에 따른 여러 문제들은 이제 지역차원에서의 적극적인 대응을 행정서비스에 요구하고 있다. 본 연구의 목적은 다문화사회의 진입에 따른 직접적인 행정서비스를 수행하고 있는 공무원들을 대상으로 그들의 다문화역량개발에 도움을 줄 수 있는 교육프로그램을 개발하기 위한 요구를 파악하기 위함이다. 다문화역량은 다문화학습의 과정을 거쳐 생겨난다고 알려져 있다. 특히 다문화역량의 구성요소에 관해서는 국가와 연구자별로 차이를 보이고 있는데, 미국의 경우 다문화역량은 문화적 의식, 등을 거쳐 습득되는 문화적 역량의 개념으로 이해되어지며, 독일의 경우 다문화역량은 의사소통능력, 갈등관리능력 등의 기초역량에서부터 문화 간의 차이 인정 능력에 이르기까지의 문화적 역량이 포괄된 역량으로 이해되어지고 있다. 본 연구의 결과로 나타는 현재의 공무원들의 다문화역량에 대한 의식수준은 전반적으로 평균수준이며 결핍된 역량에 대한 교육요구는 상당히 높다고 볼 수 있다. 특히 적지 않는 공무원들이 자기 자신의 변화가 우선적으로 중요하다고 답변한 것은 프로그램의 기획에 있어 시사하는 바가 적지 않다고 볼 것이다. The purpose of this study is to make basic materials of education programs helping competency development for public officials who are conducting public service directly, as the multi-cultural society has come. The variety of issues came after the changeable and globalized social environment such as globalization in labor industry, international marriage and introduction of diverse religion. Those are in need of active responses to public service. We recognize the need of education programs that fit in the multi-cultural paradigm, then it can possibly be used for building curriculum and education system of multi-cultural competency development as basic materials. We have to promote a learning culture for the proper response to the multi-cultural society. It can be helpful to enter a mature civil society when we recognize and embrace identities of minority groups through an education for public officials who directly provide the multi-cultural public service.

이탈리안 라이그래스의 성숙종자 유래 캘러스로부터 효율적인 식물체 재분화

우현숙,이상훈,이동기,김진수,원성혜,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass (Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plant regeneration were evaluated. MS medium containing 5 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency was observed at above 70% when embryogenic calli induced were transferred to N6 medium supplemented with 1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media tested, MS and M6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. 'Jeanne' showed the highest regeneration frequency of 73%. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.

Bacillus stearothermophilus KJ16이 생산하는 Cyclodextrinase의 정제와 효소특성

김병우,권현주,유동주 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

Cyclodextrinase from B. stearothermophilus KJ16 that can produce both cyclodextrin(CD) glucanotransferase and cyclodextrinase was purified 87.6-fold with 7% yield by ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex G-100 chromatography, and FPLC. The molecular weight of the purified enzyme was about 68,000 dalton by SDS-PAGE. The optimal pH and temperature were 6.0 and 55℃, respectively. The enzyme was stable at 50℃ for 2 hr and in the pH range of 5.5 and 8.5. The enzyme activity was inhibited strongly by mercaptoethanol, dithiothreitol, p-chloromercuribenzoate, N-bromosuccinimide, Cu^+2 and Hg^+2. The purified enzyme hydrolyzed CDs with γ-CD$gt;β-CD$gt;α-CD. The enzyme also hydroylzed linear maltodextrins and polysaccharieds, but the rates of hydrolysis for such substrates were slow as compared to that for γ-CD. The final degradation products with all substrates were maltose and glucose. Maltose was not further hydrolyzed.

21C 신지식인 양성을 위한 EQM 시스템 구축

최병태,이형민,전상봉,서승교,이우언,백종현 대구산업정보대학 2001 논문집 Vol.15 No.-

The aim of this paper is to form and to operate EQM, to say education quality management. In order to achieve the above stated purpose, the paper hypothesizes that there are similarities between enterprises and colleges in quality management. The basic method of the paper is systematic approach, modifies TQM, which is total quality management. The result is that EQM program must form and operate the feedback system for teaching and learning effect, the PSMT(project, self-study, media, team unit style teaching and learning) as a new teaching and learning method, and the digital system as an advanced mechanism, in education quality management.

도시지역 한방의료이용자의 질병양상 조사연구

강영우,조태현,김병하,남철현,조남춘,안창수,이재홍 慶山大學校 保健福祉硏究所 1997 保健福祉硏究 Vol.1 No.-

To provide the basic data for improving of curative measures in oriental medicine and for developing of health education and preventive program after understanding of classification of diseases in oriental medicine users, questionnaire interview was carried out with city dwellers, over 20 years old, selected randomly in big cities from 2nd Oct. to 11th Nov. 1995. The results were summarried as follows: 1. In the general characteristics of subjects, the highest portion of each part was 61.2% of female, 25.5% of thirties, 28.5% of high school graduate, 63.3% of middle class and 11.8% of lower class in economic status. 31.9% was housewife, 73.5% was merried and 55.1%, 38.1% came from big or small cities each. 2. By 21 classification of diseases, 19.7% was blood disorders, 17.6% was pregnancy & delivery complications and 15.2% was endorine & nutritional disorders. 3. As for age 22.6% of endocrine & nutritional disorders in twenties, 20.8%, 28.1%, 27.8% of blood & defence mechanism disorders in thirties, forties, sixties each and 24.9% of pregnancy & delivery complications in fifties were the higher than the other groups. 4. By educational level, 47.8%, 30.5% of blood & defence mechanism disorders in uneducated and middle school graduate were the higher than the other groups. 5. As for occupation, 20.8%, 32.9% of blood & defence mechanism disorders in housewives and sales each, were the higher than the other groups. 6. By economic status, 20.2% of blood & defence mechanism disorders in middle and high class, were the higher than the other groups. 7. By marital status, 21.3% of endocrine & nutritional disorders in singles, 22.0%, 25.7% of blood disorders in having partners, were the higher than the other groups. 8. As for living areas, 18.2% of pregnancy & delivery complications in big city dwellers, 21.3% of endocrine & nutritional disorders in town dwellers were the higher than the other groups. The distribution of diseases in oriental medicine users wes very different by chracteristics according the results. So It's important and essential project to improve curative technics and to develop.

Bacillus stearothermophilus KJ16이 생산하는 Cyclodextrin Glucanotransferase의 정제와 효소특성

김병우,김광현,남수완,권현주,송승구,윤종원 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

Cyclodextrin glucanotransferase from B. stearothermophilus KJ16 that can produce both cyclodextrin glucanotransferase and cyclodextrinase was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex G-100 chromatography, and FPLC. The molecular weight of the purified enzyme was about 65,000 dalton by SDS-PAGE. The optimal pH and temperature were 6.0 and 60℃, respectively. The enzyme was stable at 50℃ for 1 hr and in the pH range of 5.5 and 8.5. Mercaptoethanol and dithiothreitol inhibited the enzyme activity strongly. The enzyme produced 60% cyclodextrin(CD) from 5% soluble starch with the ^α-, ^β-, ^γ-CD ratio of 42 : 46 : 12. Amylopectin was the most suitable with 67% conversion to CD.

재생골재 콘크리트 보강블록을 이용한 증설벽의 구조성능

김선우,이갑원,박완신,한병찬,최창식,윤현도 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

The use of recycled aggregate concrete is increasing faster than the development of appropriate design recommendations. Also noises and shocks caused remodeling and reinforcement work do harm to residents. This paper reports limited experimental data on the structural performance of shear wall used concrete blocks made in recycled aggregates. Reinforced concrete frame and shear wall were tested to determine their diagonal cracking and ultimate shear behavior. The variable in the test program was the existence of infilled wall used concrete blocks Made in recycled aggregates. Based on the experimental results, Infilled wall has a high influence on the maximum strength and initial stiffness of reinforced concrete frame. Structural performance of specimen WSB is quite different from RCF specimen, particularly strength, stiffness and energy dissipation capacity.

자가 골수 중간엽줄기세포와 생분해성 고분자 스캐폴드를이용한 조직공학적 인공혈관 제조

조승우,김동익,박희정,임상현,전오주,김수현,김영하,최차용,이민재,김종성,장인성,김병수 대한혈관외과학회 2003 Vascular Specialist International Vol.19 No.2