Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Sokho Kim,Myung-Hoon Oh,Bum-Seok Kim,Won-Il Kim,Ho-Seong Cho,Byoung-Yong Park,Chul Park,Gee-Wook Shin,Jungkee Kwon 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been suffi- ciently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to 200mM) for 1 h before treatment with 1 mg/ mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Kim, Sokho,Oh, Myung-Hoon,Kim, Bum-Seok,Kim, Won-Il,Cho, Ho-Seong,Park, Byoung-Yong,Park, Chul,Shin, Gee-Wook,Kwon, Jungkee The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been sufficiently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to $200{\mu}M$) for 1 h before treatment with 1 mg/mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

플라즈마 표지소자의 제작

이상윤,라병욱,박동수,황인헌,이덕동,신영남,박성배,이동욱,박용석,박형근,손상호,권태근,채경락,정경득 慶北大學校 自然科學大學 1986 自然科學論文集 Vol.4 No.-

An Ac-type Plasma Display Panel (PDP) operating with Ne-Ar(0.1%) Penning mixture gas is fabicated. The characterics of the panel with electrodes covered with thin and thick dielectric layers are studied. The brightness of the Neon-orange light emitted by the panel measured as function of applied voltage and frequency. As an application, a graphic display system equipped with PDP showing still and moving pictures is made.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

여성 하부요관석환자에서 무마취하 요관경하 배석술

박영호,송윤섭,임용순,김영호,이남규,구자현,서병욱,김민의,전윤수 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Although ureteroscopy(URS) has been used widely to remove ureteral calculi, there is an argument against the belief that anesthesia or admission is needed for URS. We evaluated the experience of URS without anesthesia in female patients. From September 1997 to July 1998, URS was performed in 31 female patients without anesthesia. EHL(Electrohydraulic Lithotripsy) was used in 21 patients with impacted or large stone over 5mm. All patients were given oral antibiotics for 3 days and follow-up KUB was taken to confirm successful removal of the stone one week after the procedure. Successful treatment was defined as complete removal of the stone or residual fragments less than 2mm. Overall success rate was 96.7%(30 cases among 31 cases). All but one patient tolerated with analgesia through the entire procedures. There were only minimal complications such as mild flank pain and hematuria. So, we recommend that non-anesthetic ureteroscopic removal of stone as the first choice for treatment of lower ureteral stones in female based on the minimal morbidity, high success rate and low cost.

기관지천식에서의 Mabuterol의 임상효과

박인원,최병휘,허성호 최신의학사 1989 最新醫學 Vol.32 No.3

치환성 양이온이 중금속 흡착에 미치는 영향

박병윤,허남호,이정재 효성여자대학교 부설 한국환경위생연구소 1991 환경위생연구 Vol.1 No.1

국내산 zeolite의 중금속 제거제로서의 이용가능성을 검정하기 위해 zeolite에 의한 중금속의 흡착능을 조사하였다. Zeolite의 주구성점토광물은 clinoptilolite와 smectite였다. 증금속 ion들의 흡착반응은 45분 이후에 흡착평형에 도달하였다. 이들의 흡착량은 첨가중금속 ion들의농도가 증가할수록 증가하는 경향이었으며 흡착등온선은 Freundlich식에 잘 부합되었다. 치환성 양 ion별 중금속 흡착량은 Na^+ -> K^+ -> Mg^+2 -> Ca^+2 -zeolite의 순으로 1가 양Ion으로포화된 시료가 2가 양Ion으로 포화된 시료보다 더 많은 양의 중금속을 흡착하였다. This study was conducted to examine the adsorption capacity ofheavy metal cations by zeolite for the purpose of investigating the possibilityfor heavy metal cations eliminator of Korean zeolite. The dominant clay minerals of zeolite were clinoptilolite and smectite. The adsorption of heavy metal cations by zeolite reached equilibrium after 45 minutes. The adsorption increased with increasing concentrations of the bulk solution, and it was found that the adsorption isothermal curves conformed to Freundlich's equation. Samples saturated with monovalent cations adsorbed more heavy metal cations than those saturated with divalent cations, and the order of adsorption amounts was Na^+ -> K^+ -> Mg^+2 -> Ca^+2 -saturated samples.

NATM 터널간의 굴착거리에 따른 터널 거동에 관한 연구

박춘식,장정욱,안병호 국립7개대학공동논문집간행위원회 2004 공업기술연구 Vol.4 No.-

This thesis studied the behavior of tunnels according to the change in the excavation distance during the additional construction of NATM tunnels. The Finite Difference Method was employed to analyze the crown settlement, the horizontal convergence, and the axial force of rock bolts, extending the distance between the tunnels up to five times as long as the diameter of tunnel. It turned out that the crown settlement, the horizontal convergence, and the axial force of rock bolts became decrease as the distance between the tunnels became farther, and that they converged when the distance between the tunnels was over 3D.

웹기반의 가축전염병 발생자료 관리시스템(AIMS) 개발

박병호,김석재,황인진,김시동,남향미,위성환 한국농업정보과학회 2003 농업정보과학 Vol.4 No.1

The AIMS(Anima1 Infectious Disease Data Management System) was developed to collect and analyze data on animal infectious disease outbreak (62 diseases). When an animal infectious disease is confirmed at a diagnostic laboratory, the disease outbreak is then reported to the regional government through AIMS. After the regional government confirms the number of animals infected or dead, outbreak date and etc, this information is reported to the Ministry of Agriculture & Forestry and the National Veterinary Research & Quarantine Service through AIMS. The AIMS analyzes the outbreak data and reports the outbreak statistics by region, month and year to them. In addition, the AIMS can identify the farm where there was an outbreak of the animal infectious disease. There are four user roles : 1) Administrator, responsible for the overall operation of the system. 2) Inspector, responsible for confirmation of the animal infectious disease outbreak. 3) Pathologist, responsible for input to an animal infectious disease at first. 4) Gallery, only able to read the outbreak statistics. The AIMS has been implemented using the ASP technology and is operated on the web to allow easy access. The data, middle and presentation tier are based on MS-SQL 2000 server, Microsoft transaction server and Internet Explorer, respectively. The database's stored procedure was used to improve the performance. In addition to above software, the dextupload software is used to upload the files. By using the AIMS, a more accurate statistics for the animal infectious disease outbreak is expected.

夏節期中의 琴湖江의 水質調査 硏究

朴炳允,染成鎬,李松愛 대구효성가톨릭대학교 1991 연구논문집 Vol.42 No.1

In order to porvide the basic information for the conservation of Keumho river, pH, DO, BOD, COD, nitrate, phosphate and heavy metals were investigated at seven sampling sites(Yeungchun dam, Yeungseo bridge, Hayang bridge, Ayang bridge, Seobyun bridge, Paldal bridge, Gangchang bridge). The results were as follows. 1. The values of DO at other sampling sites except for the downstream of Yeungchun dam were lower than those of saturated DO, and, particularly, DO sag was observed at Gangchang bridge. 2. The value of BOD at Gangchang bridge was 13.3ppm, which exceeded the fourth grade of environmental criterion(8ppm). 3. The calue of COD at the downstream of Yeungchun dam was 2.1ppm, but the value of COD at Gangchang bridge, the most highly polluted site, was 27.6ppm, which exceeded the fifth grade of environmental criterion(8ppm). 4. The values of NH₃-N at Yeungseo bridge and Gangchang bridge were 1.93ppm and 1.94ppm, respectively, but those of other sampling sites were at range of 0.18∼0.60ppm The values of NO₃-N at seven sampling sites were at gange of 3.74∼16.32ppm. These values were very high in comparison with those of NH₃-N. 5. The contents of heavy metals at Gangchang bridge(Cu 0.27ppm, Cd 0.034ppm Mn 0.081ppm, Fe 1.742ppm, Zn 0.684ppm, Cr 1.018ppm) were higher than those of other sampling sites.