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Characterization of the European type of maternal lineage evident in extant Jeju native pigs
Byeong‐Woo Kim,In‐Cheol Cho,Moon‐Sung Park,Tao Zhong,임현태,Sung‐Soo Lee,Hee‐Bok Park,Moon‐Suck Ko,Jun‐Heon Lee,Jin‐Tae Jeon 한국유전학회 2011 Genes & Genomics Vol.33 No.2
Using a partial D‐loop sequence of mtDNA, an intensive analysis was conducted of the maternal lineages of Jeju native pigs (JNPs) from Korea. In total, 100 mtDNA sequences were obtained from Asian wild boars (AWBs), European wild boars (EWBs), Asian domestic pigs (ADPs), European domestic pigs (EDPs), and JNPs and were used for phylogeny and network analyses. Two distinct JNP groups - one (JNPA) in the Asian cluster and the other (JNPE) in the European cluster - were identified in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the closest to that of EWB and a clear haplogroup was identified that shared an identical haplotype (hap04) among 15 individuals of JNPE and 2 individuals of EWB. A Landrace and an EWB shared hap03with a JNPE. EWB, Landrace, Large White, and Duroc formed two clear haplogroups with JNPE in a parsimonious medianjoining network analysis, suggesting that an obvious maternal contribution of EDP has occurred in JNPE in recent years. A pair‐wise mismatch analysis also indicated that JNPE may have experienced a sudden population expansion, suggesting a more recent establishment compared with the gradual population expansion of JNPA. The JNPE group therefore should be further evaluated in order to decide whether this group should be culled or accepted into further programs for maintenance of the JNP population as a pure breed.
유병철,박종문,이영주,윤두훈 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-
이 연구는 포도의 생육기간에 사용되는 봉지의 특성이 포도의 생육과 당도에 어떤 영향을 미치는지 알아보고자 수행되었다. 포도의 생장에 영향을 미치는 많은 요소가 있지만 빛의 영향을 가장 많이 받는 것으로 알려져 있다. 그래서 미국과 유럽의 경우 다른 경제적인 이유도 있지만, 포도가 빛에 그대로 노출되도록 봉지를 씌우지 않는 무대재배를 한다. 국내의 경우는 식용 생과의 생산이 목적이기 때문에 포도의 생장기간에 병충해방지 및 과실의 착색효과 및 외관보호 등 여러 목적을 가지고 봉지를 씌워서 재배를 한다. 봉지의 구조에 따라서 포도의 생육과 당도에 영향을 미치게 되므로, 봉지를 만드는 원료인 섬유의 종류와 고해정도를 달리해서 봉지를 제작하여 포도를 생산했다. 실험실에서 제조한 종이로 재배한 포도의 당도가 기존에 사용되던 봉지로 재배된 포도보다 당도가 약 0.7 Brix 정도 높았다. 또한 침엽수 천연섬유와 침엽수 재생섬유, 침엽수와 활엽수 섬유를 혼합하여 만든 종이와 활엽수 섬유로 만든 종이가 빛의 투과성과 동도에 상반된 결과를 보였으며, 봉지가 빛을 많이 흡수할수록 당도가 높음을 알 수 있었다. 봉지내부의 온도변화를 측정한 결과 포도의 생육에 적정한 온도가 있음을 추정할 수 있었다.
Arabidopsis ubiquitin-specific protease 6 (AtUBP6) interacts with calmodulin
Moon, Byeong-Cheol,Choi, Man-Soo,Kang, Yun-Hwan,Kim, Min-Chul,Cheong, Mi-Sun,Park, Chan-Young,Yoo, Jae-Hyuk,Koo, Sung-Cheol,Lee, Sang-Min,Lim, Chae-Oh,Cho, Moo-Je,,Chung, Woo-Sik Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
Calmodulin (CaM), a key Ca^(2+) sensor in eukaryotes, regulates diverse cellular processes by interacting with many proteins. To identify Ca^(2+)/CaM-mediated signaling components, we screened an Arabidopsis expression library with horseradish peroxidase-conjugated Arabidopsis calmodulin2 (AtCaM2) and isolated a homolog of the UBP6 deubiquitinating enzyme family (AtUBP6) containing a Ca^(2+)-dependent CaM-binding domain (CaMBD). The CaM-binding activity of the AtUBP6 CaMBD was confirmed by CaM mobility shift assay, phosphodiesterase competition assay and site-directed mutagenesis. Furthermore, expression of AtUBP6 restored canavanine resistance to the △ubp6 yeast mutant. This is the first demonstration that Ca^(2+) signaling via CaM is involved in ubiquitin-mediated protein degradation and/or stabilization in plants. ⓒ 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
<i>Arabidopsis</i> ubiquitin-specific protease 6 (AtUBP6) interacts with calmodulin
Moon, Byeong Cheol,Choi, Man Soo,Kang, Yun Hwan,Kim, Min Chul,Cheong, Mi Sun,Park, Chan Young,Yoo, Jae Hyuk,Koo, Sung Cheol,Lee, Sang Min,Lim, Chae Oh,Cho, Moo Je,Chung, Woo Sik Elsevier 2005 FEBS letters Vol.579 No.18
<P><B>Abstract</B></P><P>Calmodulin (CaM), a key Ca<SUP>2+</SUP> sensor in eukaryotes, regulates diverse cellular processes by interacting with many proteins. To identify Ca<SUP>2+</SUP>/CaM-mediated signaling components, we screened an <I>Arabidopsis</I> expression library with horseradish peroxidase-conjugated <I>Arabidopsis</I> calmodulin2 (AtCaM2) and isolated a homolog of the UBP6 deubiquitinating enzyme family (AtUBP6) containing a Ca<SUP>2+</SUP>-dependent CaM-binding domain (CaMBD). The CaM-binding activity of the AtUBP6 CaMBD was confirmed by CaM mobility shift assay, phosphodiesterase competition assay and site-directed mutagenesis. Furthermore, expression of AtUBP6 restored canavanine resistance to the Δ<I>ubp</I>6 yeast mutant. This is the first demonstration that Ca<SUP>2+</SUP> signaling via CaM is involved in ubiquitin-mediated protein degradation and/or stabilization in plants.</P>
Moon, Byeong-Cheol,Choo, Byung-Kil,Cheon, Myeong-Sook,Yoon, Tae-Sook,Ji, Yun-Ui,Kim, Bo-Bae,Lee, A-Young,Kim, Ho-Kyoung The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.1
Definitive identification of original plant species is important for standardizing herbal medicine. The herbal medicines Cynanchi Wilfordii Radix (Baekshuoh in Korean and Beishuwu in Chinese) and Polygoni Multiflori Radix (Hashuoh in Korean and Heshuwu in Chinese) are often misidentified in the Korean herbal market due to morphological similarities and similar names. Therefore, we developed a reliable molecular marker for the identification of Cynanchi Wilfordii Radix and Polygoni Multiflori Radix. We used random amplified polymorphic DNA (RAPD) analysis of three plant species, Polygoni multiflorum, Cynanchum wilfordii, and Cynanchum auriculatum, to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed six sequence characterized amplification region (SCAR) markers for distinguishing Polygoni Multiflori Radix and Cynanchi Wilfordii Radix. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. These SCAR markers can be used for efficient and rapid authentication of these closely related species, and will be useful for preventing the distribution of adulterants.
RAPD 분석을 통한 대황(大黃)과 종대황(種大黃) 감별용 SCAR 유전자 마커 개발
문병철 ( Byeong Cheol Moon ),이영미 ( Young Mi Lee ),천진미 ( Jin Mi Chun ),이아영 ( A Young Lee ),윤태숙 ( Taesook Yoon ),전명숙 ( Myeong Sook Cheon ),추병길 ( Byung Kil Choo ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: Due to the morphological similarity and frequent occurrence of intermediate forms as well as morphological variations of aerial part, the correct identification between Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma is very difficult. To develop a reliable method for correct identification and improving the quality standards of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma, we analyzed RAPD and developed SCAR marker. Methods: To amplify target DNA at the genomic level, 32 Operon 10-mer random primers were applied with four Rheum species, R. officinale, R. palmatum, R. tanguticum and R. undulatum. The nucleotide sequences were determined and species-specific primers were prepared depending on the species-specific RAPD amplicons after subcloned into the pGEM-Teasy vector. To develop the SCAR markers, species-specific PCR amplification and mutiplex-PCR were carried out using the single species-specific primer pairs and combinations of them, respectively. Results: We used RAPD analysis of four Rheum plant species to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed two SCAR markers that amplified 314 bp and 390 bp DNA fragments in only R. undulatum but not in R. officinale, R. palmatum, R. tanguticum and R. undulatum, for distinguishing Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. Conclusions: These genetic markers can be used for the efficient discrimination of plants species and commercial herbal medicines between Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma, to ultimately prevent indiscriminate distribution and prescription of these herbal medicines.