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Kim, Bu-Yeo,Lee, Jun,Park, Sung Joon,Bang, Ok-Sun,Kim, No Soo Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P><I>Descurainia sophia</I> has been traditionally used in Korean medicine for treatment of diverse diseases and their symptoms, such as cough, asthma, and edema. Our previous results showed that ethanol extract of the seeds of <I>D. sophia</I> (EEDS) has a potent cytotoxic effect on human cancer cells. In this study, we reveal the molecular events that are induced by EEDS treatment in A549 human lung cancer cells. The dose-dependent effect of EEDS on gene expression was measured via a microarray analysis. Gene ontology and pathway analyses were performed to identify functional involvement of genes regulated by EEDS. From gene expression analyses, two major dose-dependent patterns were observed after EEDS treatment. One pattern consisted of 1,680 downregulated genes primarily involved in metabolic processes (FDR < 0.01). The second pattern consisted of 1,673 upregulated genes primarily involved in signaling processes (FDR < 0.01). Pathway activity analyses revealed that the metabolism-related pathways and signaling-related pathways were regulated by the EEDS in dose-dependent and reciprocal manners. In conclusion, the identified biphasic regulatory mechanism involving activation of signaling pathways may provide molecular evidence to explain the inhibitory effect of EEDS on A549 cell growth.</P>
Kim, Bu-Yeo,Suh, Kyung-Suk,Lee, Je-Geun,Woo, Seon Rang,Park, In-Chul,Park, Sun-Hoo,Han, Chul Ju,Kim, Sang-Bum,Jeong, Sook-Hyang,Yeom, Young Il,Yang, Suk-Jin,Kim, Chang-Min,Cho, Su Jin,Yoo, Young Do,Ch Raven Press 2012 Annals of Surgical Oncology Vol.19 No.suppl3
<P>The tissue environment in the region of hepatocellular carcinoma (HCC) influences both vascular invasion and recurrence. Thus, HCC patient prognosis depends on the characteristics not only of the tumor but also those of adjacent surrounding liver tissue.</P>
Kim, Bu-Yeo,Kim, Minjeong,Jeong, Ji Seong,Jee, Sun-Ha,Park, Il-Hyun,Lee, Byung-Chul,Chung, Sun-Ku,Lim, Kyung-Min,Lee, Yun-Sil Elsevier 2019 Environmental research Vol.173 No.-
<P><B>Abstract</B></P> <P>Bisphenol A (BPA), a synthetic monomer commonly included in the daily products, has a structure similar to the estrogen receptor agonist. Therefore BPA has been anticipated to interfere with the hormone metabolisms and cause diverse pathological conditions. But the effects of BPA on the genetic landscapes of liver or hepatic cells have not been fully established. Gene expressional changes induced by low- or high-dose of BPA were evaluated in 3D cultured human hepatoma cells (HepG2 spheroids) <I>in vitro</I> at 0, 0.5, 5 and 200 μM and liver of rats exposed to BPA at 0, 0.5 and 250 mg/kg for 90 days <I>in vivo</I>. Functional enrichment analysis, pathway activity measurement and network analysis were performed using BPA-responsive genes. Treatment with BPA changed a lot of gene expressions in both HepG2 spheroids and rat livers depending on doses of BPA. Functional enrichment and pathway analysis show that lipid or steroid metabolism-related functions were altered by BPA in both HepG2 spheroids and livers of rats. Lipid metabolism-related functions altered by BPA formed a large cluster encompassing lipid biosynthesis, steroid metabolic process and cholesterol regulation process. It was also observed that distribution of pathway activities was correlated between HepG2 spheroids and rat livers at low-dose of BPA. Distance distribution in protein-protein interaction network also evidenced the closeness of BPA-responsive genes to metabolism pathways which include lipid metabolism. Collectively, we demonstrated that BPA greatly influenced overall gene expression and biological functions in both human hepatoma spheroids and rat liver, in which lipid- or steroid metabolism-associated genes were significantly altered by the exposure to BPA.</P> <P><B>Highlights</B></P> <P> <UL> <LI> BPA induced transcriptomic changes associated with lipid and sterol metabolism in HepG2 spheroids and rat liver. </LI> <LI> Functional enrichment, pathway, pathway activity and protein-protein interaction network analyses coherently support low dose effects of BPA on lipid metabolism. </LI> <LI> The effects on lipid metabolisms are well in line with human metabolomics and epidemiological studies. </LI> <LI> The altered lipid metabolism pathways by BPA were demonstrated in both human cells and animals. </LI> </UL> </P>