배추(Brassica campestris L. var. pekinensis Makino)의 cDNA library 구축 및 상동성 비교

안주미,권오병,전봉균,이풍연,이정민,권무식 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

Expressed sequence tag(EST) has a good value to discover a new gene or to study its structure. Some 20 ESTs were generated to obtain new genetic resources of chinese cabbage(Brassian campestris L. var. pekinensis Makino). Poly A+ RNAs were isolated from 10-day-old seedlings grown at 25℃ under the day light. cDNA gene bank was constructed using λ ZAP /cDNA synthesis /Giga Pack Gold Packing kit. About a million clones were able to obtain from the library. All the clones examined so far had insert DNAs. Nucleotide compositions of randomly selected clones were determined by the Sanger mthod. The DNA sequences were compared with those deposited in the GenePept and GenBank database to figure out nucleotide homologies. Two ESTs showed significant similarities to the enlisted sequences. They are chloroplast GADPH subunit of Arabidopsis thaliana and carbonic anhydrase of A. thaliana. The full DNA sequences of the two clones are being determined. The cDNA gene bank constructed. in this experiment will being used to isolate more genes induced by the light in the plant.

가시오가피의 부위별 물 추출물이 흰쥐의 혈관이완반응에 미치는 영향

姜奉錫,金亨煥,安德均,崔湖榮 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

In this study we have examined the actions of water extract of Eleutherococus senticosus (Rupr. et Maxim.) Maxim. (ESH), Stem, Root bark (RB) and Root xylem (RX) in isolated thoracic and abdominal aorta assay. To compare the vasodilation effect, put. ESH, Stem, RB and RX into them which vessels were precontracted by 5-hydroxytryptamine (5-HT). We found out the thoracic aorta segments were responded to ESH with a dose-dependent vasodilation. The 5-HT induced contraction at 10-4M were relaxed after addition of the 2.0 ㎎/㎖ ESH as 32.1% (Stem), 34.9% (RX) and 87.2% (RB). The 5-HT induced vasocontraction at 10^-4M were vasodilated such as 87.2% in thoracic aorta and 89.0% in abdominal aorta with 2.0㎎/㎖ ESH. Addition of 2.0㎎/㎖ ESH in thoracic aorta which was precontracted by the 5-HT at 10-4M were come out differently 0% (water fraction), 53.8% (butanol fraction) and 96.6% (ethyl acetate fraction). The 5-HT induced contraction at 10-4M with and without endothelium were inhibited by 87.2% and 87.8% after addition of the ESH (2.0 ㎎/㎖). In conclusion, ESH-induced relaxation in the isolated rat thoracic aorta and abdominal aorta were come out be dose-dependent relaxation, and ESH's ethyl acetate fraction was showed the potent vasodilation effect.

허혈양상화, Adenosine 및 Pinacidil이 허혈 및 재관류후 흰쥐넙다리곧은근에서 Superoxide dismutase발현에 미치는 영향

백두진,유영미,황규성,안동춘,정호삼,강봉균 대한체질인류학회 2000 해부·생물인류학 (Anat Biol Anthropol) Vol.13 No.1

본 실험은 흰쥐에서 나타나는 허혈양상화의 효과와 그 발생기전을 규명하고자 하였다. 실험동물로는 300-350g 내외의 숫흰쥐를 사용하였으며 온엉덩동맥을 2시간 결찰한 후, 3시간, 6시간, 12시간, 24시간 및 72시간 재관류시켰으며, 온엉덩동맥을 5분간 결찰하고 5분간 재관류시키는 과정을 3회 반복하여 허혈양상화시키거나 시술적전 adenosine 혹은 pinacidil을 투여하고 같은 방법으로 허혈 및 재관류시켰다. 8-cyclopentyl-1,3-dipropylxanthine 혹은 glibenclmide 투여하고 허혈양상화시킨 다음 허혈 및 재관류시키고 넙다리곧은근을 적출하여 파라핀절편을 제작하고 in situ hybridization 조직화학법으로 Cu, Zn 및 Mn-SOD mRNA의 발현을 비교관찰하여 다음과 같은 결과를 얻었다. 1. 모든 실험군 넙다리곧은근에서 Cu, Zn 및 Mn-SOD mRNA는 단면적이 작은 근육섬유에서만 발현되었다. 2. 정상대조군 넙다리곧은근에서 Cu, Zn- 및 Mn -SOD mRNA는 약하게 발현되였다. 3. 허혈 및 재관류군에서는 재관류 시간경과에 따라 넙다리곧은근에서 CU, Zn-SOD mRNA는 중등도로 발현되었고, Mn-SOD mRNA는 재관류 3시간 및 6시간 경과시 약하게 혹은 중등도로 발현되었으며 재관류 12시간 경과시 약하게 혹은 미약하게 발현되었고, 24시간 경과시에는 중등도로 발현되었으며 72시간 경과시에는 약하게 혹은 중등도로 발현되었다. 4. 허혈양상화 처치로 넙다리곧은근에서는 Cu, Zn-SOD mRNA의 발현이 증가하여 12시간 경과시 중등도로 발현되었고 24시간 경과시 약하게 혹은 중등도로 발현되었으며 72시간 경과시에는 약하게 발현되었다. Mn-SOD mRNA 는 처치후 12시간 경과시 중등도로 발현되였고 24시간 경과시 중등도 혹은 강하게 발현되었으며 72시간 경과시 약하게 발현되었다. 5. 허혈양상화 허혈 및 재관류군에서 Cu, Zn-SOD mRNA는 재관류 6시간까지 중등도로 발현되었으며 재관류 12시간 및 24시간 경과시에는 약하게 혹은 중등도로 발현되였고 72시간 경과시에는 약하게 발현되였다. Mn-SOD mRNA는 재관류 12시간까지 중등도로 발현되었고 24시간 경과시 중등도 혹은 강하게 발현되었으며 72시간 경과시에는 약하게 발현되었다. 6. Adenosine 투여후 허혈 및 재관류시에는 넙다리곧은근에서 Cu, Zn-SOD mRNA는 재관류 12시간 경과시까지 중등도로 발현되었고 재관류 24시간 경과시에는 약하게 혹은 중등도로 발현되였으며 재관류 72시간 경과시에는 약하게 발현되었다. 7. Pinacidil 투여후 허혈 및 재관류시에는 넙다리곧은근에서 Cu, Zn-SOD mRNA는 재관류 6시간까지 중등도로 발현되었고 재관류 12시간 경과시에는 약하게 혹은 중등도로 발현되었으며 재관류 24시간 및 72시간 경과시에는 약하게 발현되었다. 8. 8-cyclopentyl-1,3-dipropylxanthine 투여후 허혈양상화 허혈 및 재관류시 넙다리곧은곤에서 Cu, Zn-SOD mRNA는 재관류 3시간까지 중등도로 발현되였고 재관류 6시간 및 12시간에는 약하게 흑은 중등도로 발현되었으며 재관류 24시간 및 72시간 경과시에는 약하게 발현되였다. Mn-SOD mRNA를 재관류 3시간까지 약하게 발현되었고 재관류 12시간까지 중등도 혹은 약하게 발현되였으며, 재관류 24시간 경과시에는 약하게 발현되었고 재관류 72시간 경과시에는 중등도로 발현되었다. 9. Glibenclamide 투여후 허혈양상화 허혈 및 재관류시에는 넙다리곧은근에서 Cu, Zn-SOD mRNA는 재관류 24시간까지 중등도로 발현되었고, 재관류 72시간 경과시까지는 약하게 혹은 중등도로 발현되었다. Mn-SOD mRNA는 재관류 기간을 통하여 중등도로 발현되었다. 이상의 소견을 종합하면 허혈 및 재관류시 Cu, Zn- 및 SOD mRNA의 발현은 증가하였고 허혈양상화로 특히 Mn-SOD mRNA의 발현이 증가하였으며, 허혈양상화의 효과는 adenosine A_(1) 수용체와 K_(ATP) 통로의 자극과 밀접한 연관이 있는 것으로 결론 지을 수 있다. A brief episode of ischemia and reperfusion termed 'ischemic preconditioning' has been established as rendering muscle tolerance to damage during a subsequent prolonged ischemia. The effects of ischemic preconditioning in the cardiac muscle are related to the stimulation of adenosine A, receptor and the opening of KATP channel. The effect and mechanisms of ischemic preconditioning in the skeletal muscle are not known clearly. The superoxide radical injures the skeletal muscle during the ischemia and reperfusion. There are two types of SOD, which metabolizes the superoxide radicals to H_(2)O_(2) and O_(2), in the cell. One of them is Cu, Zn-SOD in the cytoplasm and the other is Mn-SOD in the mitochondria. The activities of SOD are increased against the formation of superoxide radical during the reperfusion. The author performed the present study to investigate the effect and the mechanisms of ischemic preconditioning by measuring the expression of SOD mRNA on timely reperfused ischemic muscles. The healthy Sprague-Dawley rats weighing from 300g to 350g were used as experimental animals. Under pentobarbital(50 mg/kg) anesthesia, lower abdominal incision was done and left common iliac artery was occluded by vascular clamp for 2 hours. Rectus femoris muscles were obtained respectively at 3, 6, 12, 24 and 72 hours after reperfusion. The ischemic preconditioning group underwent three episodes of 5minute occlusion and 5minute reperfusion of common iliac artery followed by 2hours of ischemia and timely reperfusion. Adenosine (50 ㎍/kg) or pinacidil(1 mg/kg) was administered intravenously before ischemia. 8-cyclopentyl-1,3-dipropylxanthine(15 mg/kg) or glibenclamide(0.5 mg/g) was administered intravenously before ischemic preconditioning. Paraffin sections with 4 ㎛ thickness in all groups were obtained. The expression of Cu, Zn- and Mn-SOD mRNA was observed by use of in situ hybridization. The results obtained were as follows. I. The expression of SOD mRNA was seen only in small muscle fibers of the rectus femoris muscle of the rat. 2. Weak expressions of Cu, Zn- and Mn-SOD mRNA were observed in the normal control rat. 3. After 2 hours of ischemia, moderate expression of Cu, Zn-SOD mRNA was observed until 72 hours of reperfusion. Weak or moderate expression of Mn-SOD mRNA at 3 hours and 6 hours of reperfusion, weak or trace expression at 12 hours of reperfusion, moderate expression at 24hours of reperfusion and weak or moderate expression at 72hours of reperfusion were observed. 4. After ischemic preconditioning, moderate expressions of Cu, Zn-SOD mRNA were seen in the groups of 3, 6, 12 and 24 hours of reperfusion. Moderate expressions of Mn -SOD mRNA were seen in the group of 0, 3, 6 and 12hours of reperfusion and strong expression was seen in the group of 24hours of reperfusion after ischemic preconditioning. 5. After 2 hours of ischemia with ischemic preconditoining, moderate expressions of Cu, Zn-SOD mRNA were seen in the groups of 0, 3, 6, 12, 24 hours of reperfusion. Moderate expressions of Mn-SOD mRNA were observed in the groups of 0, 3, 6, and 12 hours of reperfusion and moderate or strong expression was seen in the group of 24 hours of reperfusion. 6. After 2 hours of ischemia with the pretreatment of adenosine, moderate expressions of Cu, Zn-SOD mRN A were seen in the group of 0, 3, 6, 12 and 24hours of reperfusion. Moderate expression of Mn-SOD mRNA in the groups and 3 hours of reperfusion, strong expression in the group of 6 and 12 hours of reperfusion and moderate expression in the group of 24hours of reperfusion were seen. 7. After 2 hours of ischemia with the pretreatment of pinacidil, moderate expressions of Cu, Zn-SOD mRNA were seen in the groups of 0, 3, 6 and 12 hours of reperfusion and those of Mn-SOD mRNA were seen in the groups of 3, 6, 12 and 24 hours ofreperfusion. 8. After 2hours of ischemia with ischemic preconditioning and the pretreatment of 8 -cyclopentyl- l,3dipropylxanthine, moderate expression of Cu, Zn-SOD mRNA were observed in the groups of 0, 3, 6. and 12hours of reperfusion and those of Mn-SOD were seen in the groups of 6, 12 and 72hours of reperfusion. 9. After 2hours of ischemia with ischemic preconditioning and the pretreatment of glibenclamide, moderate expressions of CU, Zn-and Mn-SOD mRNA were seen in all groups of reperfusion. Consequently, these results suggest that the expression of Cu, Zn and Mn-SOD mRNA increases during 2hours ischemia and reperfusion with or without ischemic preconditioning. The effects of ischemic preconditioning are closely related to the stimulation of adenosine A₁receptor and K.ATP channel.

Protein Kinase C Inhibitor Reduces Barrier Repair Response by Increasing Intracellular Free Calcium

( Bong Kyun Ahn ),( Se Kyoo Jeong ),( Hee Sung Kim ),( Jeong Taeg Seo ),( Sung Ku Ahn ),( Seung Hun Lee ) 대한피부과학회 2004 초록집 Vol.42 No.20

여드름 환자의 삶의 질 조사

안봉균 ( Bong Kyun Ahn ),이상주 ( Sang Ju Lee ),남궁기 ( Ki Namkoong ),정예리 ( Yae Lee Chung ),이승헌 ( Seung Hun Lee ) 대한피부과학회 2005 대한피부과학회지 Vol.43 No.1

여드름의 병인

안봉균 ( Bong Kyun Ahn ),최응호 ( Eung Ho Choi ),이승헌 ( Seung Hun Lee ) 한국피부장벽학회 2002 한국피부장벽학회지 Vol.4 No.1

필러 시술 금기와 제품 사용설명서에 대한 고찰

안봉균 ( Bong Kyun Ahn ),이수근 ( Soo-keun Lee ),김혜리 ( Hyeree Kim ),김혜성 ( Hei Sung Kim ) 대한피부과학회 2017 대한피부과학회지 Vol.55 No.7

Filler injection is an extremely popular cosmetic procedure in Korea, but surprisingly few dermatologists are aware of the package inserts in filler products. In the era of legal dispute, it is important that we fully understand the indications, contraindications, and side effects of the individual filler products. The purpose of this paper is to provide a general overview of the contents of package inserts of filler products that are commercially available in Korea, especially in terms of the contraindications of filler injection. The authors emphasize that greater improvement in the Korean package inserts of filler products can be expected. Currently, practicing physicians are usually blamed for filler complications. We also found many significant translational errors. We hope that dermatologists take more interest in filler package inserts by reading this overview. (Korean J Dermatol 2017;55(7):389∼393)

산발성으로 발생한 점상 수장족저 각피증 1예

안봉균,천승현,이원수 대한피부과학회 2003 대한피부과학회지 Vol.41 No.12

Punctate palmoplantar keratoderma(PPK) is a rare disease, characterized by small, hard hyper-keratotic papules which are irregularly distributed on the palms and soles. PPK is an autosomal dominant disease with variable penetrance. We report a case of sporadic case of punctate palmo-plantar keratoderma in a 26-year-old female patient. (Korean J Dermatol 2003;41(12):1694~1696)

Outlines of Geology of the Kanghwa Island

Bong Kyun Kim(金鳳均),Yong Ahn Park(朴龍安) 대한지질학회 1968 지질학회지 Vol.4 No.1

The Origin of the so-called Punch Bowl

Bong Kyun Kim(金鳳均),Yong Ahn Park(朴龍安) 대한지질학회 1967 지질학회지 Vol.3 No.1