과일과피로부터 폴리페놀 분리에 따른 생리기능연구

안봉전,이진태,곽재훈,박정미,이진영,박태순,손준호,최청 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

Biological activities and anticarcinogenicity of Korean Pear peel were investigated. Electron donating activity and superoxide dismutase(SOD)-like activity of fraction Ⅱ, Ⅲ were up to 90% and 50-60% at 50ppm, respectively. Inhibitory effects on xanthine oxidase were about 80% at 50ppm, breast adenocarcinoma was about 60% at 2,000 ppm, higher Ⅲ than Ⅱ. Inhibitory effect on prostate adenocarcinoma was about 23% at 500 ppm. In conclusion, Korean Pear peel was expected to use as a functional material.

Benzo(a)pyrene 독성에 의한 사람 림프아 세포(NC-37)에서 c-myc 유전자의 발현

하봉준,정인철,곽충근,조무연 고신대학교 의학부 1996 高神大學校 醫學部 論文集 Vol.11 No.1-2

To investigate expression in c-myc gene by a chemical carcinogen benzo(a)pyrene(BP), human lymphoblast NC-37 cells were exposed to various concentrations of BP and the c-myc gene expression was evaluated by northern and slot blot hybridization methods. When NC-37 cells exposed to BP of 0-4㎍/ml were treated with HindⅡ/XbaⅠ restriction enzyme, the c-myc genes were cut at the same region regardless of BP concentration. The site of cleavage by the restriction enzyme, HindⅡ/XbaⅠ was identical in the control, BP-treated and BP-washed cells. However, the m-RNA expression in slot blot hybridization appeared to be 4-5 times higher in BP-treated cells than in the control, and this effect was partially removed by washing the BP. When the DNA isolated from NC-37 cells exposed to various concentrations(0, 2, 4㎍/ml) of BP were amplified by polymerase chain reaction using a primer containing c-myc exon Ⅰ, the resulting DNA were of the same size in all groups. These results suggest that overexpression of c-myc oncogene may be required for the malignant transformation and maintenance in benzo(a)pyrene poisoned human lymphoblast NC-37 cells.

한국산 약용식물의 화장품천연소재로서 응용에 관한연구

안봉전,이진태,이순애,곽재훈,박정미,이진영,박태순,손준호 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

Biological activities and application of sanguisorbae officinalis L. were investigated. In the enzymological physiological activities, the electron donating ability(EDA) was 54.92% in 10 ppm and it was over 90% over 50ppm and SOD-like activity was high as 65.36% in 1000 ppm, it was gradual increased. As inhibitory effect of xanthine oxidase, it was 17.90% in 200 ppm and a little low as 36.89% in 500 ppm and inhibitory effect of tyrosinase, it was a little low as 20.45% below 1000 ppm. As the result of measuring the lipid oxidation, all the concentrations of medical ion treatments had the ability to keep it from acidification and metal ion blocking effects about the lipid oxidation promoting factors(Fe^(2+) and Cu^(2+)), Fe^(2+) was better than Cu^(2+) and all concentrations of medical ion treatments was 40% in 50ppm. When it was applied into normal skin-softener it showed safe effect so that we can expect that as the natural material of cosmetics.

Prognostic factors associated with early mortality after surgical resection for pancreatic adenocarcinoma

Bong Jun Kwak,Song Cheol Kim,Ki-Byung Song,Jae Hoon Lee,Dae Wook Hwang,Kwang-Min Park,Young-Joo Lee 한국간담췌외과학회 2014 한국간담췌외과학회지 Vol.18 No.4

Backgrounds/Aims: Identifying pancreatic cancer patients at high risk of early mortality following surgical resection for pancreatic cancer is important to make optimal treatment decisions in multidisciplinary setting. The purpose of this study was to identify the factors related to early mortality in patients who underwent pancreatic resection for pancreatic adenocarcinoma. Methods: We reviewed our institution’s experience with all consecutive patients who underwent pancreatectomy for pancreatic adenocarcinoma from January 2000 to December 2010. One thousand patients were eligible for our study. Fifty-three patients who did not meet the study criteria were excluded. Based on 12 months after surgery, patients were divided into early mortality group or the remaining group. We performed logistic regression analysis to identify predictors of early mortality. Results: Among 947 patients who met our study criteria, 302 (31.9%) early mortality (defined as experiencing death within 12 months after surgery) occurred. Multivariate analysis revealed that patient age and surgery time period were statistically significant predictors of early mortality within six months after surgery. Poorly differentiated tumor and adjuvant chemotherapy were statistically significant predictors of early mortality within 12 months after surgery. Total pancreatectomy and lymphovascular invasion were significant (p<0.05) prognostic factors of early mortality within 6 or 12 months after surgery. Conclusions: We suggest followings to avoid early mortality after pancreatic resection: patients with multiple risk factors related to early mortality after pancreatectomy should be considered for alternative treatment; patient’s general condition and surgical technique improvement are important; and adjuvant therapy should be taken into consideration.

Laparoscopic end-to-end biliary reconstruction with T-tube for transected bile duct injury during laparoscopic cholecystectomy

Bong Jun Kwak,Ho Joong Choi,Young Kyoung You,Dong Goo Kim,Tae Ho Hong 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.96 No.6

Purpose: This report describes the laparoscopic end-to-end biliary reconstruction with T-tube for transected bile duct injury (BDI) during laparoscopic cholecystectomy. Methods: We performed a retrospective descriptive analysis for all patients with a transected BDI at a single institution. We collected and analyzed data for injury site and type, reconstruction methods, conversion rate, previous intervention, and outcomes. Results: Between January 2014 and December 2017, 2,901 patients underwent laparoscopic cholecystectomy at a single institution. Among them, 8 patients experienced a transected BDI during laparoscopic cholecystectomy, so the surgeon performed laparoscopic end-to-end biliary reconstruction with T-tube. Our patient series consisted of 6 women (75%) and 2 men (25%) with a mean age of 48.3 years (median, 49 years; range, 29–77 years). Two cases were converted to open surgery. The most common injured site was the common bile duct (5 of 8, 62.5%). The most common injury type, using Bismuth’s classification system, was type I (3 of 8, 37.5%). The mean operating time was 136.8 minutes (median, 135.0 minutes; range, 0–180.0 minutes). The mean hospital stay was 7.0 days (median, 4.5 days, range: 3.0–21.0 days). The mean follow-up was 36.4 months (median, 34.0 months; range, 16.0–63.0 months). We observed one postoperative complication during the follow-up period. The patient had an anastomosis site leakage and was cured after reoperation. Conclusion: Laparoscopic end-to-end biliary reconstruction with T-tube for transected BDI during laparoscopic cholecystectomy seems to be safe and feasible in selected patients. However, long-term follow-up to identify complications from bile duct stricture remains important

Intraoperative radiofrequency ablation and distilled water peritoneal lavage for spontaneously ruptured hepatocellular carcinoma

Bong Jun Kwak,Joonseon Park,Yong Kyong Kwon,Jung Hyun Kwon,Young Chul Yoon 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.97 No.6

Purpose: Spontaneously ruptured hepatocellular carcinoma (srHCC) is known to be a life-threatening complication with poor prognosis. Although there are various treatment modalities, there is no definite treatment guideline. The purpose of this study was to review the surgical outcome and prognosis of srHCC treated with intraoperative radiofrequency ablation (RFA) and distilled water peritoneal lavage (DWPL). Methods: From March 2012 to October 2018, 9 patients with srHCC who underwent emergent surgery were reviewed. After hematoma removal, intraoperative RFA and DWPL were applied to all patients. Hepatectomy was performed if necessary. Patients with multiple tumors, distant metastasis, and vascular tumor involvement in radiologic imaging were excluded. Results: Six of 9 patients with diameters less than 7 cm were able to obtain hemostasis using RFA alone (RFA group). However, 3 patients with a tumor size of more than 10 cm underwent liver resection because they could not obtain hemostasis with RFA (hepatectomy only group). The RFA group had shorter operation time (148.3 ± 31.7 minutes vs. 251.7 ± 20.2 minutes, P < 0.05) and less red blood cell transfusion (5.8 ± 2.5 packs vs. 24.0 ± 11.5 packs, P < 0.05) than the hepatectomy only group. There was no peritoneal metastasis at long-term follow-up in the RFA group. Five-year recurrence-free survival rate was 0% in both groups. However, 5-year overall survival rate was better in the RFA group (83.3% vs. 0%, P < 0.05). Conclusion: Intraoperative RFA and DWPL are easy to perform and theoretically the best methods for managing relatively small srHCC.

Clinical outcome of 1,000 consecutive cases of liver transplantation

Bong Jun Kwak,Dong Goo Kim,Jae Hyun Han,Ho Joong Choi,Si Hyun Bae,Young Kyoung You,Jong Young Choi,Seung Kew Yoon 대한외과학회 2018 Annals of Surgical Treatment and Research(ASRT) Vol.95 No.5

Purpose: The aim of this study was to analyze survival outcomes in 1,000 consecutive liver transplantations (LTs) performed at a single institution from 1993 to April 2017. Methods: The study population was divided into 2 groups based on donor type: deceased donor LT (DDLT; n = 181, 18.1%) and living donor LT (LDLT; n = 819; 81.9%), and into 3 periods based on the number of cases (first 300 cases, middle 300 cases, last 400 cases). Results: Infection was the most common cause of death, accounting for 34.8% (95 of 273). Mortality due to hepatocellular carcinoma recurrence occurred most frequently between 1 and 5 years after transplantation. Mortality rate by graft rejection was highest between 5 and 10 years after transplantation. And mortality by de novo malignancy occurred most frequently after 10 years after transplantation. The patient survival rates for the entire population at 5 and 10 years were 74.7%, and 68.6%, respectively. There was no difference in survival rate between the LDLT and DDLT groups (P = 0.188). Cause of disease, disease severity, case period, and retransplantation had a significant association with patient survival (P = 0.002, P = 0.031, P = 0.003, and P = 0.024, respectively). Conclusion: Surgical techniques and perioperative management for transplant patients have improved and undergone standardization. Controlling perioperative infection and managing patients with HCC as LT candidates will result in better outcomes.

Clinical outcome of 1,000 consecutive cases of liver transplantation: a single center experience

Kwak, Bong Jun,Kim, Dong Goo,Han, Jae Hyun,Choi, Ho Joong,Bae, Si Hyun,You, Young Kyoung,Choi, Jong Young,Yoon, Seung Kew The Korean Surgical Society 2018 Annals of Surgical Treatment and Research(ASRT) Vol.95 No.5

<P><B>Purpose</B></P><P>The aim of this study was to analyze survival outcomes in 1,000 consecutive liver transplantations (LTs) performed at a single institution from 1993 to April 2017.</P><P><B>Methods</B></P><P>The study population was divided into 2 groups based on donor type: deceased donor LT (DDLT; n = 181, 18.1%) and living donor LT (LDLT; n = 819; 81.9%), and into 3 periods based on the number of cases (first 300 cases, middle 300 cases, last 400 cases).</P><P><B>Results</B></P><P>Infection was the most common cause of death, accounting for 34.8% (95 of 273). Mortality due to hepatocellular carcinoma recurrence occurred most frequently between 1 and 5 years after transplantation. Mortality rate by graft rejection was highest between 5 and 10 years after transplantation. And mortality by de novo malignancy occurred most frequently after 10 years after transplantation. The patient survival rates for the entire population at 5 and 10 years were 74.7%, and 68.6%, respectively. There was no difference in survival rate between the LDLT and DDLT groups (P = 0.188). Cause of disease, disease severity, case period, and retransplantation had a significant association with patient survival (P = 0.002, P = 0.031, P = 0.003, and P = 0.024, respectively).</P><P><B>Conclusion</B></P><P>Surgical techniques and perioperative management for transplant patients have improved and undergone standardization. Controlling perioperative infection and managing patients with HCC as LT candidates will result in better outcomes.</P>

A CMOS-based Temperature Sensor with Subthreshold Operation for Low-voltage and Low-power On-chip Thermal Monitoring

Jun-Seok Na,Woosul Shin,Bong-Choon Kwak,Seong-Kwan Hong,Oh-Kyong Kwon 대한전자공학회 2017 Journal of semiconductor technology and science Vol.17 No.1

A CMOS-based temperature sensor is proposed for low-voltage and low-power on-chip thermal monitoring applications. The proposed temperature sensor converts a proportional to absolute temperature (PTAT) current to a PTAT frequency using an integrator and hysteresis comparator. In addition, it operates in the subthreshold region, allowing reduced power consumption. The proposed temperature sensor was fabricated in a standard 90 nm CMOS technology. Measurement results of the proposed temperature sensor show a temperature error of between −0.81 and +0.94°C in the temperature range of 0 to 70°C after one-point calibration at 30°C, with a temperature coefficient of 218 Hz/°C. Moreover, the measured energy of the proposed temperature sensor is 36 pJ per conversion, the lowest compared to prior works.

Punch graft and negative pressure wound therapy for surgical wounds healing after mohs micrographic surgery of bone invaded squamous cell carcinoma

( Jun Ho Kwak ),( Hoon Choi ),( Bong Seok Shin ),( Chan Ho Na ),( Min Sung Kim ) 대한피부과학회 2023 대한피부과학회 학술발표대회집 Vol.74 No.2