식도 평활근종이 동반된 Alport 증후군 1례

정진영,김철민,임연정,김자형,유종우,최보화,홍수종,박영서 대한소아과학회 2002 小兒科 Vol.45 No.9

NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

The Change of Ventricular Tachycardia by Cryoinjury on the Swine Right Ventricular Epicardium

( Bo Young Joung ),( Zheng Zhe Xu ),( Il Kwon Kim ),( Ki Chul Hwang ),( Nam Sik Chung ),( Moon Hyoung Lee ),( Sung Soon Kim ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.3

This study was performed to assess the influence of the cryoinjury on the dynamics of wavefronts and to determine whether they can convert ventricular fibrillation(VF) to ventricular tachycardia(VT) in fibrillating right ventricular(RV) of swines using an optical mapping system. A cryoinjury with a diameter of 12 mm was created on the epicardium of perfused RV of swines(n=6) and optical mapping were taken from baseline until 10 min after the cryoinjury. Out of 35 cryoinjuries, the images were possible to be interpreted in 32. The optical action potential could not be observed in either the cryoinjury or peri-injury sites at 1 and 3 min, was observed in only the cryoinjury site at 5 min, and recovered in both sites at 10 min. The cycle length of the tachycardia was 135.9±23.6 msec at baseline, 176.2±79.3 msec at 1 min, 187.6±97.9 msec at 3 min, 185.5±19.2 msec at 5 min, and 152.1±64.1 msec at 10 min. The cycle lengths at 1, 3, and 5 min after the cryoinjury were significantly more prolonged than that atbaseline (p=0.001, p=0.006, p=0.016). After the cryoinjury, the VF changed to VT in 9(28.0%), and terminated in 2(6.3%). These changes were observed mainly within 5 min after cryoinjury. The cryoinjury had anti-fibrillatory effects on the tissue with VF. This phenomenon was related to a decreasing mass and stabilizing wavefronts.

Elevated Levels of Inflammatory and Vasoactive Factors in the Culprit Coronary Artery of Acute Myocardial Infarction

( Young Guk Ko ),( Jae Hun Jung ),( Sung Ha Park ),( Eui Young Choi ),( Bo Young Joung ),( Ki Chul Hwang ),( Dong Hoon Choi ),( Yang Soo Jang ),( Nam Sik Chung ),( Won Heum Shim ),( Seung Yun Cho ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.3

Distal embolization of the thrombus and plaque debris, as well as the release of inflammatory and vasoactive factors are suggested as important factors that may jeopardize successful myocardial reperfusion during primary coronary intervention. However, there are currently few clinical data on the presence and the role of such factors. We performed in 18 acute myocardial infaction patients primary percutaneous coronary intervention(PCI) with the distal protection device of distal occlusive balloon type, PercuSurge GuideWire system. We collected blood samples from the femoral artery before PCI and from culprit coronary artery using Export aspiration catheter after predilation while inflating the distal protection balloon and after completing PCI with deflation of the distal protection balloon. We determined concentrations of C-reactive protein, soluble CD40 ligand, IL-6, serotonin, tissue factor, and factor VIIa inblood samples. The concentrations of soluble CD40 ligand(2.84±3.74 vs 0.98±0.63 ng/mL, p= 0.004), IL-6(33.67±32.63 vs 17.08±21.41 pg/mL, p<0.001), serotonin(2.05±0.76 vs 0.92±0.60 ng/mL, p<0.001), tissue factor (257.17±84.34 vs 154.60±87.99 pg/mL, p<0.001) and factor VIIa(34.30±27.30 vs 24.19±28.00 ng/mL, p=0.016) were significantly higher in the culprit coronary artery after the first predilation than those in the peripheral blood before PCI. However, the CRP concentrations in the samples from the culprit coronary artery and the femoral artery did not differ significantly(4.15±3.19 vs 4.60±3.68 mg/L, p=0.056). The blood samples from coronary artery after multiple aspirations showed reduced concentrations of soluble CD40 ligand(2.84±3.74 to 1.64±1.96 ng/mL, p=0.030), IL-6(33.67±32.63 to 22.44±21.87 ng/mL, p=0.010), serotonin(2.05±0.76 to 1.29±0.82 ng/mL, p<0.001), tissue factor(257.17±84.34 to 198.45±95.14 ng/mL, p=0.001) and factor VIIa(34.30±27.30 to 27.65±27.24 ng/mL, p=0.001) compared to those in the coronary aspirates obtained after first balloon dilation of the culprit lesions. We found increased levels of soluble inflammatory and vasoactive factors such as soluble CD40 ligand, IL-6, serotonin, tissue factor and factor VII in the aspirate from the culprit coronary artery compared to those in peripheral blood. The aspiration of blood from culprit lesion during PCI resulted in significantly reduced concentrations of inflammatory and vasoactive markers.

심방세동의 기전

정보영 ( Bo Young Joung ) 대한내과학회 2011 대한내과학회지 Vol.81 No.4

Atrial fibrillation (AF) remains the most common adult rhythm disorder, and it associated with a substantial rate of morbidity and economic burden. AF involves a wide spectrum of arrhythmias from lone AF to paroxysmal to chronic AF. It is likely that AF comprises a spectrum of disease with no single mechanism adequate enough to comprehensively explain AF and its variability. Mechanism of fibrillation is explained by multiple wavelets and focal activation theories. Electrical, contractile and mechanical remodeling is involved in AF progression. Atrial remodeling may also increase in atrial fibrosis which can slow conduction velocity and can shorten the refractory period in atria with long-standing AF. Mechanical remodeling manifests as decreased atrial contractility and increased atrial compliance which leads to a stretch of the atrial myocardium. Abnormal intracellular calcium dynamics is observed in AF. Modulating factors such as genetic factors, age, obesity, sleep apnea, inflammation, autonomic factors and atrial and pulmonary vein stretch only partially account for the increase in AF. It is still unclear whether initiation of AF activates direct inflammatory effects or whether the presence of a pre-existing systemic inflammatory state promotes further persistence of AF. Although significant progress in understanding the mechanism of this arrhythmia has been accomplished, the pathophysiology of AF is complex and likely has many possible mechanisms which may be interrelated. (Korean J Med 2011;81:417-422)

인공 장애물에 의한 심실세동의 동역학적 변화의 광학적 지도 분석

정보영 ( Bo Young Joung ),구본권 ( Bon Kwon Koo ),서정철 ( Zheng Zhe Xu ),김일권 ( Il Kwon Kim ),황기철 ( Ki Chul Hwang ),이문형 ( Moon Hyoung Lee ),김성순 ( Sung Soon Kim ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.3

The effects of artificial obstacles on the dynamics of ventricular fibrillation have been extensively investigated with an electrical mapping system. This study was performed to assess the influence of transmural obstacles on the dynamics of wavefronts and to determine whether obstacles can convert ventricular fibrillation to ventricular tachycardia by stabilizing wavefronts in fibrillating right ventricular tissues of pigs using an optical mapping system. The right ventricles of pigs(n=15) were excised and placed in a tissue perfusion system with the epicardium facing up. A hole of increasing size from 2 mm to 8 mm in diameter was created using a skin biopsy punch. Then, the other 8mm sized hole was made just beside the first hole. The changes of wavefront dynamics and the cycle length of optical action potential waves were investigated. In 14 among 20 obstacles of ten tissues, transient attachment of electrical activities along the rim of obstacles and transient rotation of wavefronts were observed. During baseline ventricular fibrillation, fibrillation cycle length was 118.5±24.7 msec and this was increased to 135.4±30.2 msec after 8mm hole, and to 159.4±47.7 msec after 2 holes(p=0.01). There was a positive correlation between the obstacle size and cycle length(r=0.43, p=0.007). In three tissues, conversion to ventricular tachycardia from ventricular fibrillation was observed after creation of two holes. Obstacles of proper size had anti-fibrillatory effects in tissues with ventricular fibrillation and this phenomenon was partly explained by the temporary attachment of wavefronts to the obstacles.

독활기생탕가미방(獨活寄生湯加味方)이 배양 척수운동신경세포에 미치는 영향

이영보 ( Young Bo Lee ),윤상학 ( Sang Hak Yun ),임양의 ( Yang Eui Lim ),박진상 ( Jin Sang Park ),정택상 ( Taek Sang Joung ),한경호 ( Kyung Ho Han ),이수경 ( Soo Kyung Lee ),신병철 ( Byung Cheul Shin ),권영달 ( Young Dal Kwon ) 한방재활의학과학회 2003 한방재활의학과학회지 Vol.13 No.4

Objectives & Methods : To evaluate the mechanism of oxidative damage by Xanthine oxidase(XO) and hypoxanthine(HX)-induced oxygen radicals, XTT assay was carried out after the cultured mouse spinal motor neurons were preincubated for 5 hours with various concentrations of XO/HX. And DNA synthesis, PKC activity and c-fos immunohistochemistry were measured to evaluate the protective effect of Dokhwalgisaeng-tang(Duhuojisheng-tang: DGT), Dokhwalgisaeng-tanggami(Duhuojisheng-tangjiawei: DGTG), Herbar Chelidoni(HC) water extract on cultured spinal motor neurons damaged by XO/HX. Results : The results were XO/HX showed the quantitative decrease of DNA synthesis, increase of PKC activity and c-fos positive cell, in proportion to concentration and exposed time on cultured spinal motor neurons and DGT, DGTG and HC showed the protective effects against decrease of DNA synthesis and increase of PKC activity and c-fos positive cell by XO/HX. Conclusions : From the above results, it is suggested that XO/HX have a neurotoxic effect on cultured spinal motor neurons and the herbs extract, such as DGT, DGTG and HC, prevent the toxicity of XO/HX effectively.

CNN 기반 한국 번호판 인식

Tang Quang Hieu,연승호(Seungho Yeon),김재민(Jaemin Kim) 한국전기전자학회 2019 전기전자학회논문지 Vol.23 No.4

자동 한국 번호판 인식 (AKLPR)은 많은 분야에서 사용된다. 이러한 응용 분야에서 ALPR은 높은 인식률과 빠른 처리 속도가 중요하다. 최근 딥러닝의 발전으로 객체 감지 및 인식의 정확도와 속도가 향상 되고 있으며, 그 결과 딥러닝이 ALPR에 적용되고 있다. 특히 합성곱신경망(Convolutional Neural Network) 기반 객체 검출기가 ALPR에 적용되었다. 이러한 ALPR은 LP 영역을 검출하는 단계와 LP 영역의 문자를 검출 및 인식하는 단계로 구분되며, 각 단계는 별도의 CNN으로 구현된다. 본 논문에서는 단일 단계 CNN으로 ALPR을 구현하는 아키텍처를 제안한다. 제안하는 방법은 높은 인식률을 유지하면서 빠른 속도로 번호판 문자를 인식한다. The Automatic Korean license plate recognition (AKLPR) is used in many fields. For many applications, high recognition rate and fast processing speed of ALPR are important. Recent advances in deep learning have improved the accuracy and speed of object detection and recognition, and CNN (Convolutional Neural Network) has been applied to ALPR. The ALPR is divided into the stage of detecting the LP region and the stage of detecting and recognizing the character in the LP region, and each step is implemented with separate CNN. In this paper, we propose a single stage CNN architecture to recognize license plate characters at high speed while keeping high recognition rate.

남녀 및 나이에 따른 심자도 변수 차이 분석

임현균,정남식,이용호,권혁찬,정보영,박용기,Lim, Hyun-Kyoon,Chung, Nam-Sik,Lee, Yong-Ho,Kwon, Hyuk-Chan,Joung, Bo-Young,Park, Yong-Ki 대한의용생체공학회 2007 의공학회지 Vol.28 No.4

Magnetocardiography (MCG) is a device to measure the magnetic field from the heart. It is a noninvasive device and takes only few minutes to record magnetocardiogram from a subject. In this study, we compared the difference of MCG data recorded from 56 normal subjects in early twenties (28 males and 28 females, mean $age=21.0{\pm}1.6$ years) and 36 elderly subjects (20 males and 16 females, mean $age=61.9{\pm}6.9$ years) for the analysis of the age and gender difference. A total of 24 parameters used in the analysis were derived from QRS complex, R-wave, T-wave, and ST-T period. As a result, seven parameters including maximum current angle and map angle showed the significant difference (p<0.01 and p<0.05, respectively) between young males and young females. Significant difference (p<0.05) between elderly males and elderly females was found from a parameter, pole distance at T-wave peak. In the comparison of age difference, seven parameters regarding current moment, pole distance, and dynamics showed the significant difference between young and elderly males. Eight parameters also showed significant difference (p<0.05) between two younger and elderly female groups. Results showed that parameters regarding current moment, pole distance, and dynamics might be changed when people get older. In conclusion, gender and age difference should be considered when MCG data are analyzed for certain parameters.

Poster Session 3 : Apoptosis and Cell Cycle Oncogenes and Tumor Suppressor Genes Virology Immunology ; HaCaT Keratinocytes are Resistant to Apoptosis via Increased Catalase Activity by Papilloma Virus E7 Infection

( Joung Hyun Shim ),( Bo Ram Rhee ),( Kyung Ae Lee ),( Chang Weon Kho ),( Seong Goo Park ),( Pyung Keun Myung ),( Sue Nie Park ),( Yong Kyung Choe ),( Do Young Yoon ) 한국생화학분자생물학회 (구 한국생화학회) 2002 생화학분자생물학회 춘계학술발표논문집 Vol.2002 No.-