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( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.
콩의 개화기 전후 공급과 수용부위의 생장반응과 수량에 대한 질소의 효과
박재홍,송범헌,이철원,손석용,김홍식,박상일 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-
This study was conducted to examine the nitrogen effects on the growth responses, the nodulation, and the distribution of dry weight of plant tissues at major growth stages before and after the flowering stage, and to evaluate the yield components and yields with different nitrogen levels. Hwangkeum cultivar(Glycine max L mereill) was cultivated under five different nitrogen levels, 0, 2, 4, 6, 8, N Kg/10a. Stem length and branching number, which were investigated with different N levels at major growth stages, were rapidly increased for 14days for before and after the flowering stage compared to the other growth stages. They were clearly higher with treatment of 2 and 4 N kg/10a than those of other N treatments. Pod number was slightly increased from the flowering to beginning maturity. It was the highest in the treatment of 2 N Kg/10a. Comparing grain number per pod with different N levels, two grains per pod was much higher in all N treatments except for the treatment of 6 N Kg/10a. Dry weight of shoot(including leaf, stem, and pod) was rapidly increased from the flowering stage to the full seed stage and then gradually increased after the full seed stage, whereas the dry weight of root(including the nodule and root) was rapidly increased until to full pod stage and then linearly decreased from the full pod stage until the beginning maturity stage. Comparing dry weight with different N levels, the dry weight of shoot was the highest in the treatment of 4 N Kg/10a, while it of root was the highest in the treatment of 2N Kg/10a. The dry weight of nodule was the highest, 13.1 Kg/10a, at full pod stage. Comparing the dry weight of nodule with different N levels, it was increased to the application amount of 4 N Kg/10a and then clearly decreased with more application amount of N than 4 Kg/10a. The yield components which were mostly affected to the yield were pod number per plant and grain number per plant. They were 57.5 and 106.1, respectively, with treatment of 4 N Kg/10a, which showed the highest yield, about 266 Kg/10a. Comparing yield index with different N levels to the control, the yields with treatment of 2 and 4 Kg/10a were increased to 21% and 34%, respectively.
콩의 개화기전후 공급과 수용부위의 무기영양성분함량 및 흡수량에 대한 질소의 효과
박재홍,송범헌,이철원,손석용,김홍식,박상일 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-
This study was conducted to examine the nitrogen effects on the uptaken amounts and utilization of mineral nutrients between source and sink tissues at major growth stages before and after the flowering stage with different nitrogen levels Hwangkeum cultivar(Glycine max L Mereill) was cultivated under five different mtrogen levels, 0, 2, 4, 6, 8 N kg/10a Comparing contents of total-N with different plant tissues at major growth stages before and after the flowering, total-N contents of leaves was gradually decreased from the flowering to the beginning maturity, while it of pod was continuously increased from the full seed to the beginning maturity Total-N content was higher in the treatment of 4 N Kg/10a that those of others The uptaken amount of N determined with different growth stage was rapidly increased maturity Higher proportion of uptaken N were into the leaves at the full seed stage, and they were transported from the leaves into pod at the beginning maturity stage Comparing major mineral elements of the leaves and the pod with different N leaves, in case of the leaves, the content of Fe, Mn, and Zn was decreased throughout the growth stages, whereas the content of Ca and Mg was decreased from the flowering to the full seed and then it was increased after the full seed stage In case of pod, the content of K, Fe, Mn, Zn, and Na was decreased from the flowering to the beginning maturity, while contest of Mg was increased.
돼지에서 정맥, 근육 그리고 경구 투여시의 enrofloxacin의 약물동태학
윤효인,김무열,박승춘,조준형,박병권,이내경,노상석,장범수,신광순,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-
In order to characterize pharmacokinetic profiles according to route of a new enrofloxacin salt form (Enrotil®), it was given to 4 healthy pigs via oral (p.o.), intramuscular (i.m.) or intravenous (i.v.) administrations at a dose rate of 5 ㎎/㎏ body weight. Enrofloxacin (ENFX) in serum was detected by bioassay using E. coli BE1186 as a test organism. The biological elimination half-lives (t_1/2(β)) of ENFX were 6.76±0.99 h (i.v.), 7.16±2.30 h (i.m.) and 11.45±3.90 h (p.o.), Volume of distribution (Vd) of enrofloxacin was 2.20±0.31 L/㎏ (i.v.), 2.52±0.60 L/㎏ (i.m.) and 1.88±0.33 L/㎏ (i.m.). Mean residence time (MRT) was 8.77±1.26 h after i.v. injection and the maximal concentration time (Tmax) following p.o. and i.m. administration was 0.76±0.09 h and 0.60±0.12 h, indicating a rapid absorption from these routes. Bioavailibility (F) was calculated as 64.1% for p.o. administration and 59.71% for i.m. injections. In summary, the newly formulated enrofloxacin salt form has shown a high water solubility, rapid absorption and large tissue distribution, suggesting a potential antibacterials for oral application on a large scale in veterinary sectors.
강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-
과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.
[PA-0014] Detection of SNPs variations in the seed storage protein gene involved in β-conglycinin
Soo-Kwon Park(Soo-Kwon Park),Jung-Kyung Moon(Jung-Kyung Moon),Sang-Beom Lee(Sang-Beom Lee),Dool-Yi Kim(Dool-Yi Kim),Mi-Suk Seo(Mi-Suk Seo),Yu-Na Kim(Yu-Na Kim),Gyu-Tae Park(Gyu-Tae Park),June-Yeol Cho 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak
Park, Wan Beom,Kwon, Nak-Jung,Choe, Pyoeng Gyun,Choi, Su-Jin,Oh, Hong Sang,Lee, Sang Min,Chong, Hyonyong,Kim, Jong-Il,Song, Kyoung-Ho,Bang, Ji Hwan,Kim, Eu Suk,Kim, Hong-Bin,Park, Sang Won,Kim, Nam Jo The Korean Academy of Medical Sciences 2016 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.31 No.2
<P>During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.</P>
Park, Wan Beom,Poon, Leo L.M.,Choi, Su-Jin,Choe, Pyoeng Gyun,Song, Kyoung-Ho,Bang, Ji Hwan,Kim, Eu Suk,Kim, Hong Bin,Park, Sang Won,Kim, Nam Joong,Peiris, Malik,Oh, Myoung-don Elsevier 2018 INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES Vol.72 No.-
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Information on the duration of replicative Middle East respiratory syndrome coronavirus (MERS-CoV) shedding is important for infection control. The detection of MERS-CoV sub-genomic mRNAs indicates that the virus is replicative. This study examined the duration for detecting MERS-CoV sub-genomic mRNA compared with genomic RNA in diverse respiratory specimens.</P> <P><B>Methods</B></P> <P>Upper and lower respiratory samples were obtained from 17 MERS-CoV-infected patients. MERS-CoV sub-genomic mRNA was detected by reverse transcription PCR (RT-PCR) and MERS-CoV genomic RNA by real-time RT-PCR.</P> <P><B>Results</B></P> <P>In sputum and transtracheal aspirate, sub-genomic mRNA was detected for up to 4 weeks after symptoms developed, which correlated with the detection of genomic RNA. In oropharyngeal and nasopharyngeal swab specimens, the detection of sub-genomic mRNA and genomic RNA did not correlate.</P> <P><B>Conclusions</B></P> <P>These findings suggest that MERS-CoV does not replicate well in the upper respiratory tract.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Middle East respiratory syndrome coronavirus (MERS-CoV) sub-genomic mRNAs indicates that the virus is replicative. </LI> <LI> Sub-genomic mRNA was detected in lower respiratory tract specimens for up to 4 weeks after symptoms developed. </LI> <LI> In upper respiratory tract specimens, the detection of sub-genomic mRNA and genomic RNA did not correlate. </LI> </UL> </P>