Effects of Extrahepatic Cholestasis on Liver and Serum β-D-Mannosidase Activities in Ethanol Intoxicated Rats

배시우,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

Liver and serum β-D-mannosidase activities were determined in ethanol intoxicated rats with extrahepatic cholestasis induced by common bile duct ligation (CBD) to manifest the biochemical background of alcohol drinking hazard under the hepatobiliary disease. Liver β-D-mannosidase activity and its Vmax value in CBD ligated rats with chronic ethanol intoxication were found to be significantly decreased than that in CBD ligation alone. However, the difference of Km value on above hepatic enzyme was not found between the experimental groups. On the other hand, serum β-D-mannosidase activity in CBD ligated rats with chronic ethanol intoxication was increased more than that in CBD ligation alone. These results indicate that the biosynthesis of the hepatic β-D-mannosidase decreases and the serum β-D-mannosidase activity increases in cholestasis combined with chronic ehtanol intoxication, reflecting damage of aggravated hapatocytic membrane. Accordingly, the resulting data supported the fact that alcoholic drinks were enzymologically harmful to the hepatobiliary disease.

요하지통 환자에서 두 개의 캐눌라를 이용한 박동성 고주파 신경근 응고술의 임상 연구

김천숙,배재영,배덕구,강규식,안기량,권진형,김지은,유시현 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Dorsal root ganglion(DRG) block by the local anesthetics and steroids which has done classically in low back pain(LBP) patients with radiculopathy has high incidence of recurrence rate and side effects of steroids. Recently a new technique of pulsed radiofrequency thermocoagulation(P-RFTC) was introduced and substituted for it because of the benefits such as relatively low recurrence rate, low risk of nerve injury and comfort during the procedure. We experienced 15 patients who had LBP with radiculopathy and no or little response to epidural steroid injection. When we performed DR ganglionotomy by P- RFTC under the C-arm guide in these patients, we tried to approach the exact DR ganglion using tow cannulas - the one for obtaining patient's subjective symptoms and injection of contrast dye, the other for making a RF lesioning after identifying the impedance of sensory and motor stimulation. In conclusion, DR ganglionotomy by P- RFTC using two cannulas in LBP patients is an easy and safe procedure with satisfactory results.

흉추 경막외강으로 침윤한 흉선종 1례 : Metstasis to Thoracic Epidural Space

최정훈,백욱성,박대곤,오경구,최천식,배상도 高麗病院 1987 高醫 Vol.10 No.-

Ultrastructural Changes of the Gemifloxacin on Achilles Tendon in Immature Rats: Comparison with those of Ciproxacin and Ofloxacin

Bae, Chun-Sik,Oh, Dong-Min,Bae, Jin-Gyu,Kim, Jong-Choon,Kim, Sung-Ho,Kim, Su-Gwan,Jeong, Moon-Jin,Kim, Youn-Shin,Lim, Sung-Chul Blackwell Science, Ltd 2006 Basic & clinical pharmacology & toxicology Vol.98 No.4

<P>Abstract: </P><P>Gemifloxacin is a synthetic fluoroquinolone antimicrobial agent that exhibits potent activity against most Gram-negative and Gram-positive organisms, and has a comparatively low chondrotoxic potential in immature animals. This study examined the effects of gemifloxacin on the Achilles tendons in immature Sprague-Dawley rats treated by oral intubation once daily for 5 consecutive days from postnatal week 4 onward at doses of 0 (vehicle), and 600 mg/kg body weight. Ofloxacin or ciprofloxacin were used for comparison. The Achilles tendon specimens were examined by electron microscopy. In comparison with the vehicle-treated controls, there were ultrastructural changes in all samples from the gemifloxacin-, ofloxacin-, and ciprofloxacin-treated rats. Degenerative changes were observed in the tenocytes, and the cells that detached from the extracellular matrix were recognizable. The degree of degenerative changes and the number of degenerated cells in the Achilles tendon were significantly higher in the treated group than in the control group. Moreover, among the quinolone-treated groups, these findings were most significant in the ofloxacin-treated group, and least significant in the gemifloxacin-treated group. It is unclear what these findings mean with respect to the possible risk in juvenile patients treated with gemifloxacin or other quinolones. However, these results show that gemifloxacin causes less changes in the connective tissue structures.</P>

복강경하 전자궁적출술과 질식 전자궁적출술의 임상적 비교

전춘식 ( Chun Sik Jeon ),원재연 ( Jae Yeon Won ),김진홍 ( Jin Hong Kim ),이춘희 ( Chun Hee Lee ),방준배 ( Jun Bae Bang ),이윤순 ( Yoon Soon Lee ),박일수 ( Il Sooo Park ) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.12

By rapid development of laparoscopec technique and equipment, laparoscopic hysterectromy(LH), even to total laparoscopic hysterectomy and laparoscopic radical hysterectomy has been possible. The objective of this study was to review the indications, outcomes, complications of laparoscopic hysterectomy in comparison with total vaginal hysterectomy(TVH) and to evaluate the value of laparoscopic hysterectomy. Initial 50 cases of laparoscopic hysterectomy were performed from September 1994 to July 1995 and 50 cases of vaginal hysterectomy were randomly chosen from March 1993 to July 1994. The mean age of LH and TVH was 45.0±6.5 and 44.6±7.15(p=0.758). The mean parity of LH and TVH was 1.78±0.76 and 3.00±1.34(p=0.0001). The most common surgical indication for LH and TVH was myoma including adenomyosis (88 % and 54 %). The most common combined procedure with LH and TVH was adnexectomy(98% and 28%). The mean operation time for LH was significantly longer than TVH(125 min vs 68 min). There were no significant differences in hospital stay(4.6 days vs 4.1 days) and estimated blood loss(134 ml vs 182 ml) between LH and TVH. The mean uterine weight of LH was significantly heavier than TVH(323 gm vs 197.6 gm). The complication rate of LH and TVH was 26 % and 12 %. Our study strongly suggest that main prerequisute for laparoscopic hysteretomy are 1) nullipara with narrow vagina and elderly women with vaginal atropy 2) the need to guarantee removal of the ovaries 3) suspected adnexal pathology, e.g. PID, endometriosis. Moderately to severely enalrged uterus certainly is not itself indication for laparosopic hysterectomy but rather make an easy to perform vaginal hysterectomy. Laparosopic pelvic surgeons make an effort to reduce operation time and complication rate by repetition of education, video sessions, and animal model training. Most consider laparosopic hysterectomy to be a substitute for total abdominal hysterectomy, not for vaginal hysterectomy. It is clear that not all patients requiring hysterectomy will benefit from the laparoscopic approach. Proper selection for laparoscopic hysterectomy will decrease the need of laparotomy and the risk of physical and mental trauma according to laparotomy will be reduced and quality of women`s life will be raised.

Mesangial 세포에서 고포도당에 의해 유도되는 insulin-like growth factor 분비 촉진작용에 대한 ginsenosides의 차단 효과

배춘식(Chun-Sik Bae),임도선(Do-Seon Lim),윤병철(Byeong-Cheol Yoon),정문진(Moon-Jin Jeong),윤경철(Kyung-Chul Yoon),박수현(Soo-Hyun Park) 한국생명과학회 2008 생명과학회지 Vol.18 No.1

인삼은 고전적으로 항당뇨 효과가 있는 것으로 보고되고 있다. Insulin-like growth factor (IGF) 역시 당뇨병성 신증의 발병 초기에 중요한 역할을 하는 것으로 알려져 있다. 이에 본 연구에서는 mesangial 세포에서 고포도당에 의한 IGF 분비에 대한 ginsenoside의 차단 효과 및 이와 관련된 신호전달계를 알아보았다. 결과는 다음과 같다. 고포도당에 의해 증가 되었던 IGF-Ⅰ 및 IGF-Ⅱ 분비 촉진 작용은 GTS, PD 및 PT 처리 시 차단되었으며, 세포 성장 촉진작용에서도 같은 효과를 볼 수 있었다. 아울러 고포도당에 의한 산화성 스트레스 증가, GSH 감소, AA 방출 증가 작용 및 PGE2 합성 증가 작용은 GTS 처리시 현저하게 차단되었으며 PD 및 PT 처리 시 역시 억제 되는 것으로 나타났다. 이상의 결과를 볼 때 mesangial 세포에서 ginsenoside는 산화성 스트레스 및 arachidonic acid 활성 경로를 억제하여 고포도당에 의한 IGFs 분비 작용을 차단하는 것으로 나타났다. Panax ginseng C. A. MEYER is one of the most widely used herbal medicines in the Asian countries and has diverse functions including anti-diabetic action. The dysfunctions of mesangial cells in hyperglycemic conditions are implicated in the development of diabetic nephropathy. Insulin-like growth factors (IGFs) are also associated with the onset of diabetic nephropathy. Thus, we examined the effect of ginsenosides against high glucose-induced dysfunction of primary cultured rat mesangial cells. In the present study, high glucose increased IGF-I and IGF-II secretion in mesangial cells. Ginsenoside total saponin (GTS) prevented high glucose-induced increase of IGF-I and IGF-II secretion in mesangial cells. In addition, GTS prevented high glucose-induced increase of lipid peroxide formation and decrease of GSH contents. GTS also ameliorates high glucose-induced increase of arachidonic acid release and decrease of prostaglandin E2. In conclusion, GTS prevented high glucose-induced dysfunction of mesangial cells via inhibition of oxidative stress and arachidonic acid pathways.

랫드에서 난소적출이 골다공증 발생에 미치는 영향

배춘식 ( Chun Sik Bae ) 한국임상수의학회 2000 한국임상수의학회지 Vol.17 No.1

Palmitic acid에 의한 간세포 사멸효과에 대한 p38 MAPK 및 JNK 관련성

배춘식(Chun-Sik Bae),박수현(Soo-Hyun Park) 한국생명과학회 2009 생명과학회지 Vol.19 No.8

포화 지방산이 인슐린 저항성 및 지방간등의 다양한 질병의 발병에 관련된다고 보고되고 있다. 그러나 간세포에서 포화지방산이 세포 사멸에 대한 효과 및 이와 관련된 신호전달계에 대해서는 거의 알려져 있지 않고 있다. 본 연구에서는 대표적인 포화지방산인 palmitic acid 처리 시 랫트 간세포 사멸에 미치는 효과와 이들이 mitogen activated protein kinases (MAPKs)와 관련되는 지를 알아보았다. 본 실험에서 palmitic acid 처리 시 간세포 성장은 억제 되었고 lactate dehydrogenase (LDH) 활성은 증가하였다. 이러한 작용은 JNK 및 p38 MAPK 억제제에 의해서 선택적으로 차단되었다. 아울러 palmitic acid에 의한 Bcl-2 발현 억제, Bax 발현 증가 작용, GSH 함량 감소작용 및 산화성 스트레스 증가작용 역시 JNK 및 p38 MAPK 억제제에 의해서 선택적으로 차단되었다. 실제로 palmitic acid 처리시 JNK 및 p38 MAPK 활성은 증가하였다. 결론적으로 palmitic acid는 간세포에서 JNK 및 p38 MAPK 활성을 경유하여 산화성 스트레스 증가를 통하여 간세포 사멸을 유도하는 것으로 나타났다. Hyperlipidemia has been reported to be associated with the development of fatty liver. Palmitic acid, a major saturated fatty acid, is involved in the development of diverse diseases. The activation of mitogen activated protein kinases (MAPKs), such as Jun N-terminal kinase (JNKs) and p38 MAPK, is implicated in the apoptosis in diverse cells. Thus, this study was conducted to investigate the effects of palmitic acid on apoptosis and its relationship between JNK and p38 MAPK in cultured rat hepatocytes. In the present study, palmitic acid (>50 uM) decreased cell proliferation and increased lactate dehydrogenase activity in hepatocytes, which was blocked by the treatment of SP600125 (a JNK inhibitor) and SB203580 (a p38 MAPK inhibitor). Indeed, palmitic acid decreased Bcl-2 expression but increased Bax expression in rat hepatocytes, which was blocked by the treatment of SP600125 and SB203580. In addition, palmitic acid decreased glutathione (GSH) content and increased lipid peroxide formation, which was blocked by the treatment of SP600125 and SB203580. Western immunoblotting analysis also revealed that palmitic acid increased JNK and p38 MAPK. In conclusion, palmitic acid induced apoptosis through oxidative stress via JNK and p38 MAPK activation in rat hepatocytes.

A comparative study of pulp vitality following vital pulpotomy using calcium hydroxide-based sealants in beagle dogs

Gil Joon Jung, Se Eun Kim, Chun-Sik Bae, Kyung Mi Shim, Seok Hwa Choi, Seong Soo Kang1 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4

Vital pulpotomy is a very useful method for disarming of canine tooth, tooth fracture, periodontitis, and malocclusion in veterinary dentistry. Calcium hydroxide is the material commonly used as a liner during vital pulpotomy. This creates a mineralized barrier by stimulating osteoblastic hard tissue repair, arrests the inflammatory response, and soothes dentin. However, the powder or mix type calcium hydroxide materials have many disadvantages due to complicated procedures for use and are hard to handle when vital pulpotomy is followed under general anesthesia in animals. This study was conducted in order to compare the effect of mix and premixed paste type calcium hydroxide as a liner in vital pulpotomy. Six beagle dogs underwent hemisection on the mesial root of the mandibular first molar and vital pulpotomy on the distal root of the first molar. On the distal root of the left and right mandibular first molar, mix type (DYCAL®, Dentsply, USA) and premixed paste type calcium hydroxide (VITAPEX®, Morita, Japan) were used as liners, respectively. Radiological evaluation was performed at immediate, 4, 12, and 20 weeks after vital pulpotomy. According to the results, all teeth had well-formed dentinal bridges, and there were no periradicular lucency, lamina dura loss, or anomalies of the pulp cavity. According to these results, on vital pulpotomy in animals, premixed paste type calcium hydroxide was easy to handle and decreased the anesthesia period due to a more convenient application procedure. A further study of many clinical cases is needed for evaluation of side effects and other problems.

Efficacy of alcohol-free cetylpyridinium chloride on periodontal disease in beagle dogs via drinking water additive and oral gel

Jae Kyong Kim, Se Eun Kim, Chun-Sik Bae, Kyung Mi Shim, Seok Hwa Choi, Soon-Jeong Jeong, Seong Soo Kang 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.1

This study was conducted in order to examine the effects of alcohol-free cetylpyridinium chloride drinking water additive and oral gel on clinical parameters related to periodontal disease in beagle dogs. This study was conducted with healthy 15 beagle dogs. Following a professional teeth cleaning procedure, dogs were divided into three groups. Dogs in the control group received nothing, those in the drinking water additive (DWA) group received 800 ml water with 15 ml of alcohol-free cetylpyridinium chloride drinking water additive daily, and those in the Oral gel (OG) group were treated with oral gel containing alcohol-free cetylpyridinium chloride and 0.05% chlorhexidine gluconate daily. Clinical parameters, including plaque index (PI), calculus index (CI), and gingivitis index (GI) were evaluated at two and four weeks. Dogs in the DWA and OG groups had significantly less plaque than dogs in the control group at two and four weeks (P<0.01, P<0.05). And, at four weeks, CI was significantly lower in the OG group compared to the control group (P<0.05). On GI, similar scores were recorded for all groups during the experimental period. No significant difference was observed between the DWA group and the OG group. The effect of alcohol-free cetylpyridinium chloride drinking water additive was similar to the result for alcohol containing cetylpyridinium chloride mouthwash reported in a previous study. The effect in control of periodontal disease was better in the OG group because of additional chlorhexidine gluconate. However, use of drinking water additive will be more convenient for owners; thus, it will be more effective for achievement of long-term results.