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Yahya, Andi Fadly,Hyun, Jung-Oh,Lee, Jae-Ho,Jung, Myung-Suk Korean Society of Forest Science 2007 한국산림과학회지 Vol.96 No.2
Rauvolfia serpentina (L.) Benth. ex Kurz is a medicinal plant and an endangered tropical rainforest plant species. Since the field cultivation that aims to fulfill the industrial needs is never accomplished, tissue culture appears to be the most feasible way to improve the quality and quantity of R. serpentina. This experiment used two kinds of explants (roots and shoots) to induce optimal root formation in different combinations of auxin and photoperiod. Each explants exhibited different responses on given treatments. Differentiated root could be produced from explants cultured in IBA 20 mg/L with and without light. The highest number of roots, root length and root weight induced from shoot explants were effective on MS medium containing IBA 20 mg/L and incubated under dark condition, while highest total weight (callus and root) from root explants cultured on MS medium supplemented 10 mg/L IBA and 10 mg/L NAA and incubated under day length (11/13 hr). The root induced from shoot explants produced the highest major alkaloid content. The highest content of ajmaline (2.17 ppm fresh weight) and reserpine (1.30 ppm fresh weight) were observed in shoot explants cultured in MS medium containing combination of IBA 10 mg/L and NAA 10 mg/L and incubated under dark condition, yohimbine (1.47 ppm fresh weight) was in the shoot explants cultured in MS medium containing NAA 20 mg/L and incubated under day length, while serpentine was absent.
Andi Fadly Yahya,현정오,이재호,정명석 한국산림과학회 2007 한국산림과학회지 Vol.96 No.2
Rauvolfia serpentina (L.) Benth. ex Kurz is a medicinal plant and an endangered tropicalrainforest plant species. Since the field cultivation that aims to fulfill the industrial needs is neverR.serpentina. This experiment used two kinds of explants (roots and shoots) to induce optimal root formationin different combinations of auxin and photoperiod. Each explants exhibited diferent responses on giventreatments. Diferentiated root could be produced from explants cultured in IBA 20 mg/L with and withoutlight. The highest number of roots, root length and root weight induced from shoot explants were effectiveon MS medium containing IBA 20 mg/L and incubated under dark condition, while highest total weightNAA and incubated under day length (11/13 hr). The root induced from shoot explants produced thehighest major alkaloid content. The highest content of ajmaline (2.17 ppm fresh weight) and reserpine (1.30pm fresh weight) were observed in shoot explants cultured in MS medium containing combination of IBA10 mg/L and NA 10 mg/L and incubated under dark condition, yohimbine (1.47 ppm fresh weight) wasin the shoot explants cultured in MS medium containing NAA 20 mg/L and incubated under day length,while serpentine was absent.
( Ohn Lwin ),( Jung Oh Hyun ),( Andi Fadly Yahya ) 한국임학회 2010 한국산림과학회지 Vol.99 No.5
This study described the general pattern of genetic variation among ten teak (Tectona grandis Linn. f.) provenances in Myanmar and determined the most suitable seed sources for the plantation program in Bago Yoma region. Seeds of ten provenances were collected to cover the whole teak natural distribution in Myanmar and planted at four trial sites in Bago Yoma region in 1998. Seven years after planting, variation was assessed for growth, morphological characteristics and their correlation with geoclimatic factors. Statistical analysis using ANOVA revealed that there were significant differences in most of the traits measured among provenances, trial sites and provenance×site interaction at five percent level. A positive significant correlation (p<0.01) was found among most of the traits. The regression analyses between all traits and geoclimatic factors indicated the existence of teak genetic variation in teak. Most of the traits were negatively correlated with the latitude while a positive significant correlation was found between longitude and C/B ratio, crown-diameter average branch angle and leaf-remain. There was no significant correlation between the mean temperature and any other traits in this study. Furthermore, growth traits and crown diameter were positively correlated with the mean annual rainfall while negative correlation was found between the geographical distance and growth traits. Results indicate that the latitudinal pattern of teak genetic variations in growth performance was attributed to the limit of mean annual rainfall. Comparative assessment showed that local provenances were generally the best and could be use as suitable seed sources for the plantation program in the Bago Yoma region.
Assessment of Teak (Tectona grandis Linn. f.) Provenance Tests in the Bago Yoma Region, Myanmar
Lwin, Ohn,Hyun, Jung-Oh,Yahya, Andi Fadly Korean Society of Forest Science 2010 한국산림과학회지 Vol.99 No.5
This study described the general pattern of genetic variation among ten teak (Tectona grandis Linn. f.) provenances in Myanmar and determined the most suitable seed sources for the plantation program in Bago Yoma region. Seeds of ten provenances were collected to cover the whole teak natural distribution in Myanmar and planted at four trial sites in Bago Yoma region in 1998. Seven years after planting, variation was assessed for growth, morphological characteristics and their correlation with geoclimatic factors. Statistical analysis using ANOVA revealed that there were significant differences in most of the traits measured among provenances, trial sites and provenance ${\times}$ site interaction at five percent level. A positive significant correlation (p<0.01) was found among most of the traits. The regression analyses between all traits and geoclimatic factors indicated the existence of ecoclinal variation in teak. Most of the traits were negatively correlated with the latitude while a positive significant correlation was found between longitude and C/B ratio, crown-diameter, average branch angle and leaf-remain. There was no significant correlation between the mean temperature and any other traits in this study. Furthermore, growth traits and crown diameter were positively correlated with the mean annual rainfall while negative correlation was found between the geographical distance and growth traits. Results indicate that the latitudinal pattern of teak genetic variations in growth performance was attributed to the limit of mean annual rainfall. Comparative assessment showed that local provenances were generally the best and could be use as suitable seed sources for the plantation program in the Bago Yoma region.