Mouse배 십이지장상피의 세포환에 미치는 전자기장의 영향에 관한 연구

이융창 慶北大學校 醫科大學 1974 慶北醫大誌 Vol.15 No.1

albino mouse embryo의 십이지장와 세포의 세포환에 미치는 자장의 영향을 관찰하기 위하여 ^3H-thymidine을 이용한 자기 방사능 법으로 고찰해 본 결과 다음과 같은 결론을 얻었다. 1. mouse embryo의 십이지장와세포의 세포환은 자장의 영향을 받는다. 2. DNA 표지지수와 분렬지수는 작아지고 3. DNA합성전기는 55%가량 길어지며 4. 이 결과로 세대시간은 정상보다 3.3시간 길어진다. Investigations were carried out on the effect of constant magnetic field on the cell cycle and duration of its phases. The effects of magnetic field on mouse embryo during first 17days of gestation were examined in the duodenal crypt cell by means of autoradiography. The decresed labelling and mitotic indices were observed. The S phase was slightly lengthened in experimental animals. Remarkable lengthening of G_1 phase was encountered in experimental animals resulting the lengthening of generation time. A new approach and the validity of the effects of magnetic field on biological systems were discussed.

Testosterone 投與한 鷄胎器官의 成長 및 相對成長에 關하여

李隆昌,陳丁煥,朴商斌,朴元學 慶北大學校 醫科大學 1968 慶北醫大誌 Vol.9 No.1

This study is to analyze the effects of testostenone on the weight gains of body weight and various organs of chick embroys, out of which growth and relative growth formula have been derived. On the 8th and 12th days of incubation, 0.5㎎ and 7.5㎎ of testosterone were injected into the chorio-allantoic membrance of each incubated egg of two groups, and each main group were devided into three more small groups such as control, 0.5㎎ injected group and 7.5㎎ injected group. Between the 12th and 18th of incubation day, a daily total of 10 treated eggs were brocken for weighing the body weights of chick embryos and six organs, namely, brain, eyeball, lung, liver, heart and kidney. The authors used Dr. Joo's formula to analyze the growth and relative growth in this study. The results are as follows: 1) Growth rate; In the 8th day group, it was observed to be increased in body weight through out three groups-control, 0.5㎎ group and 7.5㎎ group, in 0.5㎎ groups and 7.5㎎ groups of brain, lung and liver, in 0.5㎎ group of heart and in 7.5㎎ group of kidney. The others were decreased. In the 12th day group, the increase in growth rate was observed in followings; eevery group of body weight, lung and liver, control group and 7.5㎎ group of kidney. The others were decreased. 2) Specific growth rate; The increase was observed onlo in the drug injected groups of lung in 12th day group and the others were decreased. Among the others, 7.5㎎ groups of lung in 8th day group and body weight in 12th group were increased until 13th day of incubation and thereafter they were decreased. And control of lung in 12th day group was increased until 16th day of incubation and was decreased thereafter. 3) Growth gradient: The growth centers appeared as follows. a) In the 8th day group; most of them were on 12th day of incubation, except 0.5㎎ group of brain which was on between 16th and 17th, control of eyeball on 15th, 0.5㎎ group of eyeball on 13th, 0.5㎎ groups of lung and liver on 18th, and 7.5㎎ group of lung on between 17th and 18th day. b) In the 12th day group; most of them were found to be on 12th day of incubation except control and 0.5㎎ groups of eyeball on 13th, liver and control group of kidney on 18th day. 4) Coefficient of relative growth; The coefficients of relative growth to body weight were as follows: a) In the 8th day group; 7.5㎎ group of lung, liver and 0.5㎎ group of kidney were of positive allometry; the others showed negative. b) In the 12th day group;0.5㎎ group of brain and every group of liver were of positive allometry and the others were negative.

배양을 위한 심근세포 분리에 미치는 Trypsin, Collagenase와 Dimethyl Sulfoxide의 영향

박창우,이융창 영남대학교 의과대학 1987 Yeungnam University Journal of Medicine Vol.4 No.1

백서 심장조직을 trypsin과 collagenase 두가지의 효소를 사용하여 분리하면서 각 효소의 분리효과와 두가지 효소의 복합적 세포분리 효과를 조사하였으며 이와 동시에 dimethyl sulfoxide가 세포분리과정 및 단기간 배양중에 세포에 미치는 효과를 조사한 결과를 다음과 같이 요약할수 있다. 1. 백서 심장조직에서 세포를 분리할때 4℃ trypsin 18시간 전처리후 37℃ collagenase 처치한군에서 세포생존율 세포수확량 및 심근세포 생존율이 가장 높았다. 즉 단일 효소 처리보다는 효소 복합연속 처리가 더 효율적이었다 2. 37℃ trypsin 만으로 세포분리를 하였을때 세포생존율과 수확량이 가장 낮았다. 3. 백서 심장세포의 분리과정과 초기 배양과정에서 세포 회복이나 보호에 대한 DMSO의 효과는 인정되지 않았으며 오히려 세포파괴 효과가 높음을 알수 있었다. New born rat heart cells were dissociated using trypsin and /or collegenase to elucidate the dissociation efficiency of these two enzymes. And the effect of dimethyl sulfoxide during and immediately after cell dissociation was also investigated to clarify the so-called protective activity of dimethyl sulfoxide on cell performance. The results can be summarized as follows. 1. Cold trypsin 18 hours pretreatment followed by warm collagenase treatment resulted best cell viability and cell yield. 2. Single warm trypsin treatment gave the poorest result. 3. Dimethyl sulfoxide did not seem to play any protective role during or immediately after rat heart cell dissociation. It had very damaging effect on rat heart cells.

Mouse배 십이지장상피의 세포환에 미치는 전자기장의 영향에 관한 연구

이융창 慶北大學校 醫科大學 1974 慶北醫大雜誌 Vol.15 No.1

척추동물 심근세포의 분리와 배양

이융창 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.1

To develop a simple method in isolation and cultivation of cardiac myocytes, 3-day old rat hearts were used. 0.1% trypsin and 0.1 collagenase were used combiningly to achieve a high yield of viable cells. Myocardial cells were separated from endotheloid cells via differential attachment technique. Cells were cultured in Falcon flasks, Rose chambers and Leighton tubes. Changes of cell population in culture were observed and effects of enzymes were discussed.

Polyethylene Glycol 처리후에 배양한 세포의 Succinate Dehydrogenase 양상에 관하여

이융창 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.1

To device a technique to distinguish the different cell types in mixed cell population, succinate dehydrogenase staining method was used. Myocardial cells from 3day old Sprague-Dowly rats and human epidermoid cells were cultured in mixed population. Polyethylene glycol (PEG) was applied to induce cell fusion. Cells cultured in Leighton tubes were processed for succinate dehydrogenase staining. Myocardial cells and hybrid cells revealed strong positive reaction to SD staining while HEP-2 cell revealed negative reaction. Myocardial cells and hybrid cells differed in that hybrid cells had finer, and more homogenously distributed mitochondria. The merits and the mechanisms of reaction were discussed.

심근세포 및 내피세포의 분화에 미치는 Dimethyl Sulfoxide의 영향

이동협,박이태,한승세,이융창 영남대학교 의과대학 1988 Yeungnam University Journal of Medicine Vol.5 No.2

효소와 기계적인 힘을 이용하여 얻어진 심장세포를 심근세포와 내피세포를 분리하여 48시간 배양한후 양군으로 나눠서 관찰하였다. 실험군은 배양중에 10% DMSO에 1시간 처리하였고 DMSO를 처리하지 않고 계속 배양한 것을 대조군으로 하였다. DMSO효과를 관찰하기 위하여 도립현미경하에서 세포의 형태, 수축능력, 증식 능력등을 관찰하였고, 심근세포에서는 succinate dehydrogenase 반응으로 사립체의 증감을 대조군과 비교하였고, 심장내피세포에서는 thiamine pyrophosphatase의 반응으로 Golgi의 양을 대조군으로 비교하였다. 실험결과를 요약하면 다음과 같다. 1. DMSO에 의하여 심근세포는 분화된 형태로 모양이 길어졌고 사립체의 증가에 의해 succinate dehydrogenase의 반응이 대조군보다 강하게 나타났다. 2. DMSO에 의해 심근세포는 근세섬유의 파괴와 혼란이 초래되었고 이로 인하여 수축능력이 감소되었다. 3. 심장내피세포는 DMSO에 의해 세포증식이 감소되고 고유기능이 강조되어 Gologi의 표지효소인 thiamine pyrophosphatase의 반응이 대조군보다 강하였다. 4. 심장내피세포는 DMSO의 세포접착력 소실과 운동성 소실작용에 의해 배양중 많은 세포의 손실이 초래되었다. To elucidate the effects of dimenthyl sulfoxide on myocardial and endothelial cells in culture, the cells were exposed to 10% dimenthyl sulfoxide in culture medium for 1 hour at 48 hours after cell isolation. The general morphology and the cytochemical reaction of marker enzymes for mitochondria and Golgi complexes were investigated. The results were summarized as follows : 1. DMSO induced elongation and narrowing of the cells and increase of mitochondrial reaction in myocardial cells. 2. DMSO induced destruction and disruption of myofibrils in myocardial cells resulting in increase of contractile activities. 3. In the endothelial cells, DMSO suppressed proliferative activities but thiamine pyrophosphatase reactions were enhanced indicating increase of Golgi complex activity. 4. DMSO seemed to hamper with the adhesiveness and motility of the endothelial cells causing the decrease of the number of cells in vitro.

병아리 십이지장와 상피세포의 세포환에 관한 연구

이융창,강성익,최형달,정호문 中央醫學社 1973 中央醫學 Vol.25 No.1

Tritiated thymidine autoradiography has been applied to the duodenal progenitor population (crypts of Lieberkuhn) in one day postnatal male chick to determine 1) the cell cycle of the progenitor cell pool, 2) the characteristics of DNA synthetic index, and the 3) mitotic index. Available amounts of colchicine were injected into the animals before sacrifice to arrest the dividing cell nucleus at metaphase. The results of this investigation are as follows: 1) The generation time was 14 hours, 2) DNA synthetic index was 23.3%, and 3) The mitotic index was 1.8%. The cause of shortening of DNA synthetic phase and the lengthening of post mitotic phase were discussed.

韓國人?兒體量의 相對成長에 關한 硏究

李隆昌,蔡康燮,金泰煥,金一雲 최신의학사 1969 最新醫學 Vol.12 No.10

The authors analyzed relative growth of body length, body weight, breast circumference and head circumference of Korean infant by way of relative growth formula of Huxley and Teissier. The results were as follows: 1) In male, body weight was positive allometry while breast circumference and head circumference showed negative allometry to body length. 2) In female, they were as similar as in boys. 3) The relative growth of every portion of female was negative allometry to male.

배양중 세포의 융합을 통함 분화세포의 탈분화

이융창 최신의학사 1987 最新醫學 Vol.30 No.10