치루의 후향적 분석과 임상적 고찰

이용직,이미옥,김성수,이영택,박용기,최창록 대한대장항문학회 1999 Annals of Coloproctolgy Vol.15 No.4

국가식품정책측면에서의 채소, 과일 공급 프로그램

이용직 한국식생활문화학회 2020 한국식생활문화학회학술대회논문집 Vol.2020 No.10

발효 균주 탐색을 위한 목장 주변 토양에서 분리한 내염성 미생물의 다양성 및 효소 생산능 분석

이용직,신기선,이상재 한국산업식품공학회 2020 산업 식품공학 Vol.24 No.1

The diversity and characterization of microorganisms isolated from the soil around several ranches in Korea were confirmed in this study. To isolate halophilic microorganisms, the marine agar medium was basically used and cultivated at 37oC for several days. After single colony isolation, a total of 116 pure colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rDNA sequencing, indicating that isolated strains were divided into 4 phyla, 23 families, 30 genera and 51 species. To confirm whether isolated strain can be a candidate for the fermentation of diverse food ingredients, amylase, lipase, and protease enzyme assays were performed individually, showing that 92 strains possessed at least one enzyme activity. Especially 4 strains, identified to Jonesia quinghaiensis (isolate name: JSF 19-2), Halomonas alkaliantarctica (isolate name: JSF 21), Bacillus velezensis (isolate name: NWFY-36), and Staphylococcus capitis subsp. urealyticus (isolate name: MSY-5), showed all enzyme activity tested. Moreover, 17 strains showed the ability for auxin production. This result indicated that isolated strains have shown the possibility of the application for the food and feed industries. Therefore, this study has contributed to securing domestic biological resources and the improvement of hydrolytic enzyme activity by using isolated strains.

Stimulation of Alpha-1-Adrenergic Receptor Ameliorates Obesity-Induced Cataracts by Activating Glycolysis and Inhibiting Cataract-Inducing Factors

이용직,장유나,김현민,한윤미,서홍석,엄영섭,송종석,정지훈,정태우 대한내분비학회 2022 Endocrinology and metabolism Vol.37 No.2

Background: Obesity, the prevalence of which is increasing due to the lack of exercise and increased consumption of Westernizeddiets, induces various complications, including ophthalmic diseases. For example, obesity is involved in the onset of cataracts. Methods: To clarify the effects and mechanisms of midodrine, an α1-adrenergic receptor agonist, in cataracts induced by obesity, weconducted various analytic experiments in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a rat model of obesity. Results: Midodrine prevented cataract occurrence and improved lens clearance in OLETF rats. In the lenses of OLETF rats treatedwith midodrine, we observed lower levels of aldose reductase, tumor necrosis factor-α, and sorbitol, but higher levels of hexokinase,5’-adenosine monophosphate-activated protein kinase-alpha, adenosine 5´-triphosphate, peroxisome proliferator-activated receptordelta, peroxisome proliferator-activated receptor gamma coactivator 1-alpha, superoxide dismutase, and catalase. Conclusion: The ameliorating effects of midodrine on cataracts in the OLETF obesity rat model are exerted via the following threemechanisms: direct inhibition of the biosynthesis of sorbitol, which causes cataracts; reduction of reactive oxygen species and inflammation; and (3) stimulation of normal aerobic glycolysis.

열녀정부인여산송씨묘비

이용직,박기양 전북역사문화학회 2009 전라북도금석문대계 Vol.3 No.-

위암세포 사멸에 미치는 겨우살이 추출물과 항암제의 효과

이용직,허수학,신동규,강성구,김일명,김태희 대한위암학회 2008 Journal of gastric cancer Vol.8 No.3

목적: 여러 암 치료의 보조 및 대체요법으로서 겨우살이 추 출물은 주로 유럽지역에서 이십세기 초부터 널리 사용되 었고 현재 국내에서도 항암 대체 치료제로 점차 사용하는 추세이나 그 항암 기전은 아직 명확하게 밝혀져 있지 않 다. 본 연구는 겨우살이 추출물이 위암에 미치는 영향을 세포주 실험을 통해 규명하였다. 대상 및 방법: 위암 세포주는 SNU-719 세포주를 사용하였 고 처리한 겨우살이 추출물은 (주)한국 아브노바사로부터 제공받은 ABNOBAviscum-Q와 ABNOBAviscum-F 두 가지를 사 용하였다. 겨우살이 추출물과 함께 처리한 항암제는 5-FU 와 Cisplatin을 사용하였다. CCK-8 assay kit를 사용하여 세포 생존율을 측정하였고 젖산탈수소효소(LDH) assay kit로써 세 포 사멸률을 측정하였다. Caspase 3 assay kit를 이용하여 세포자멸사에 관여하는 caspase 3의 활성 변화를 알아보았 고 Western blot analysis를 통해 세포자멸사에 관여하는 Bcl2 와 p53, 그리고 항암 작용을 하는 PTEN의 단백질 발현량을 측정하였다. 결과: 겨우살이 추출물 Q와 F를 각각 단독 처리한 실험군 보다 항암제인 5-FU와 cisplatin을 병합처리 하였을 때 세포 생존율은 더 낮아졌다. Caspase 3의 활성은 겨우살이 추출 물 및 항암제 5-FU 처리를 함으로써 대조군에 비해 4∼6배 까지 증가하였다. Bcl2의 단백질 발현량은 대조군보다 겨우 살이 추출물과 항암제 처리를 통해 감소하였고 두 약물을 함께 처리하였을 경우 더 낮아졌다. p53의 발현은 겨우살 이 추출물 처리에는 영향을 받지 않았고 항암제 처리를 통 해서만 증가되었다. 또한 항암 작용을 하는 PTEN의 단백 질 발현 정도는 겨우살이 추출물 처리를 통해서 의미 있 는 변화가 없었다. 결론: 겨우살이 추출물과 항암제인 5-FU 및 cisplatin 등을 병합 처리 할 경우 위암 세포 사멸에 있어 상승효과를 보 였다. 본 연구결과에서 겨우살이 추출물의 항암효과는 caspase 3의 활성화와 Bcl2 발현의 감소를 통해 세포자멸사를 유발하며 이 세포자멸사 유도 기전은 p53과는 상관없음(p53- independent)을 알 수 있었다.

Tubularized Penile-Flap Urethroplasty Using a Fasciocutaneous Random Pedicled Flap for Recurrent Anterior Urethral Stricture

이용직,Byung Kwon Lee 대한성형외과학회 2012 Archives of Plastic Surgery Vol.39 No.3

This report describes the use of a tubularized random flap for the curative treatment of recurrent anterior urethral stricture. Under the condition of pendulous lithotomy and suprapubic cystostomy, the urethral stricture was removed via a midline ventral penile incision followed by elevation of the flap and insertion of an 18-Fr catheter. Subcutaneous buried interrupted sutures were used to reapproximate the waterproof tubularized neourethra and to coapt with the neourethra and each stump of the urethra, first proximally and then distally. The defect of the penile shaft was covered by advancement of the surrounding scrotal flap. The indwelling catheter was maintained for 21 days. A 9 month postoperative cystoscopy showed no flap necrosis, no mechanical stricture, and no hair growth on the lumen of the neourethra. The patient showed no voiding discomfort 6 months after the operation. The advantages of this procedure are the lack of need for microsurgery,shortening of admission, the use of only spinal anesthesia (no general anesthesia), and a relatively short operative time. The tubularized unilateral penile fasciocutaneous flap should be considered an option for initial flap urethroplasty as a curative technique.

제주도 항구 인근 토양으로부터 호기성 호염성 세균의 분리 및 특성 분석

이용직,간밧 다리마,정가을,신기선,이상재 한국산업식품공학회 2022 산업 식품공학 Vol.26 No.3

To isolate aerobically and identify the diversity of halophilic bacteria in the soil around two ports, Daepopogu and Hwasun Port, on Jeju island, a total 46 halophilic bacteria strains were isolated and phylogenetically analysed. The isolated strains were divided into 3 phyla, 8 families, 16 genera and 23 species. The main taxa was the Bacilli class, which included 50.0% of the strains with 3 families, 10 genera and 15 species of Bacillaceae, Exiguobacterium_f and Planococcaceae. The second taxa was the Gammaproteobacteria class, which included 45.7% of the strains with 4 families, 5 genera and 7 species of Aeromonadaceae, Halomonadaceae, Marinobacteraceae and Vibrionaceae. The isolated strains were tested for hydrolytic enzymes, amylase, lipase and protease activity, and 31 strains showed activity of at least one enzyme. Furthermore, auxin activity was determined in 7 strains. This study showed that the isolated strains have possible applications in the food and agricultural industries and have importance as a genetic resource in Korea.

연골조직공학에서 Polylactic-Glycolic Acid와 Chitosan 골격의 비교

이용직,정호윤,신동필,김종엽,양정덕,이동걸,박재우,조병채,백봉수 대한성형외과학회 2005 Archives of Plastic Surgery Vol.32 No.5

Clinical application of the cartilage formed by tissue engineering is of no practical use due to the failure of long-term structural integrity maintenance. One of the important factors for integrity maintenance is the biomaterial for a scaffold. The purpose of this study is to evaluate the difference between polylactic-co-glycolic acids (PLGA) and chitosan as scaffolds. Human auricular chondrocytes were isolated, cultured, and seeded on the scaffolds, which were implanted in the back of nude mice. Eight animals were sacrificed at 4, 8, 12, 16, and 24 weeks after implantation respectively. In gross examination and histological findings, the volume of chondrocyte-PLGA complexes was decreased rapidly. The volume of chondrocyte-chitosan complexes was well maintained with a slow decrease rate. The expression of type II collagen protein detected by immunohistochemistry and western blots became weaker with time in the chondrocyte-PLGA complexes. However, the expression in the chondrocyte-chitosan complexes was strong for the whole period. Collagen type II gene expressions using RT-PCR showed a similar pattern. In conclusion, these results suggest that chitosan is a superior scaffold in cartilage tissue engineering in terms of structural integrity maintenance. It is expected that chitosan scaffold may become one of the most useful scaffolds for cartilage tissue engineering.

[토론문] 4차 산업혁명시대, 우리의 영어교육은 어디로 가고 있나?

이용직 한국문화융합학회 2017 한국문화융합학회 전국학술대회 Vol.2017 No.12