未搗精麥類 混食이 白鼠成長과 脂質代謝에 미치는 影響

柳總根,李成東,高鎭福 최신의학사 1975 最新醫學 Vol.18 No.8

Some effect on the growth rate and lipid metebolism in growing albino rats by feeding on rice diet mixed with unpolished wheat and barley have been studied. The rice diet and 30% unpolished wheat and barley mixed with rice diets for 8 weeks. The species of unpolished wheat and barley used in this experiment were the wheat, the barley and the naked barley. The results obtained are summarized as follows: 1. As for the growth, the unpolished wheat and barley mixed with rice diet groups were much lower than that of the rice diet group. 2. The contents of food consumption showed the similar tendency to each other diet group, and the food efficiency rate and the protein effeciency rate of the unpolished wheat and barley mixed with rice diet groups were lower than that of the rice diet group. 3. Liver and brain weights were no differences in each diet groups. 4. The total cholesterol content in brain of the rice diet group were increased than that of the unpolished wheat and barley with rice diet group, and the lipid content in liver were no differences in each group. 5. The free fatty acid and the lecithin content in liver, brain and serum of the rice diet group were higher than that of the unpolished wheat and barley with rice diet groups. Being taken into consideration of the above facts it was acquired that the growth rate and lipid metabolism were affected by the unpolished wheat and barley with rice diet.

週期的 高蛋白質食餌 給食에 依한 臟器內 酵素活性 變動에 關한 硏究

柳總根 고려대학교 의과대학 1972 고려대 의대 잡지 Vol.9 No.1

This study was planned to observe the effect on change of enzyme activity by periodic feeding of high protein diet on rats maintained with low protein diet. The experimental animals were 136 female albino rats from pure strain, weighing 239-297gm. The animals were divided into five diet groups: the standard diet group and four of the rice diet supplemented with fish flour groups, once every other day (A group), every two days (B group), every three days (C group) and every four days (D group). The above each group was re-divided into four groups by feeding term of each diet: the terms were 15 days, 30 days, 45 days and 60 days. The animals were sacrificed after feeding each diet for the corresponding period and glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT) activities in the liver and pancreas, cholinesterase activity and acetylcholin content in the liver and serum were determined. The results obtained are summarized as follows: 1. The activity of GPT in the respect of experimental diet, tends to decrease in A,B,C and D groups compared with the control group, and in the degree, A group is more remarkable and D group is slighter than any other group. By the feeding terms, 15 days feeding group shows the minimum value and the 60 days feeding group shows the close value to that of the control group. 2. The activity of GOT in the liver is apt to decrease in each diet group and activities in A and B groups are near to the control group, while between D and the control group, some difference is made. 3. The activities of GPT and GOT in the pancreas of each diet group show a tendency to decrease, for those in the control groups, but the difficulty is found to describe the diference in regular tendency among three diet groups. And in feeding term group, GPT activity in the pancreas is similar to a tendency to change of that in the liver i.e, that in the 15 days feeding group is the minimum, and that of the 60 days feeding group is close to that in the control group. 4. The activity of cholinesterase in the liver is higher in all of the supplemented diet group than in the control group, and that in the 15 days feeding group is increased the most remarkable and that in the 30 days feeding group is increased in the minimum degree. 5. The activity of cholinesterase in the serum is found to be increased in all of the supplemented diet group, compared with the control group, as well as in the liver. 6. The contents of acetylcholine in the liver and serum is increased in each diet and feeding term groups, compared with the control group.

週期的 高蛋白質食餌 給食에 依한 臟器內 酵素活性 變動에 關한 硏究

柳總根 고려대학교 의과대학 1972 고려대학교 의과대학 잡지 Vol.9 No.1

不均衡食餌에 依한 白鼠體內 Homeostasis에 對한 硏究

柳總根(Tcheong Kun Ryu) 韓國營養學會 1974 Journal of Nutrition and Health Vol.7 No.2

數種 蛋白質添加 給食時 白鼠臟器中 SH group 및 Cholesterol 含量

高鎭福,柳總根,崔田道,李成東 최신의학사 1972 最新醫學 Vol.15 No.12

The effect of various protein sources on the contents of sulfhydryl group in liver and cholesterol in plasma and liver of rats were observed in this study. Experimental animal, 72 male albino rats, 45±5 day of after birth were used. Either rice or wheat was made as a basic diet, and whole milk powder, egg powder, fish flour (anchovy flour) and soybean flour of 5% level, respectively, was supplemented to each basic diet as protein source. Each animal group was fed on the corresponding diet for 96 days, and then the sulfhydryl group in the liver and the cholesterol in the liver and plasma were determined. The results are summarized as follows: 1. The sulfhydryl group contents in the liver shows no difference between each group of protein supplemented, and between groups of basic diets and protein supplemented. 2. The total cholesterol contents in the liver does not change reqularly as each protein source is supplemented, to rice, but increase, to wheat diet. 3. The free cholesterol contents in the liver increases through each protein supplement to basic diets. 4. The total cholesterol in the plasma is not influenced by each protein supplemented, and groups of protein supplemented show similar contents to group of either rice or wheat. The above results leads to the conclusion that the cholesterol contents in the liver and plasma more or less affected by the kinds of protein source.

감자의 成分分析 및 감자食餌가 白鼠營養에 미치는 影響

崔永鍾,柳總根 최신의학사 1983 最新醫學 Vol.26 No.8

肝癌細胞의 γ-Glutamyltranspeptidase에 對한 Monoclonal antibody에 關한 硏究

金明坤,柳總根 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.2

This study was devised to produce monoclonal antibody against hepatocelullar carcinoma speclfic γ-glutamyltranspeptidase (HCC GGT), which proceeded by first purifying the HCC GGT using ion exchange chromatography and immunoaffinity chromatography. Subsequently, a mouse was immunized with the purified enzyme, and then spleen cells taken from the immunized mouse was fused with mouse myeloma cells (SP2-Ag 14) to form the hybridoma. The following results were obtained by characterization of the monoclonal antibody of the hybridoma using immunoglobulin isotyping and immunoblot. 1. The purification of HCC GGT by means of ion exchange chromatography and immunoaffinity chromatogiaphy produced a yield of 29.41%, a purification fold of 167.87, and final specific activity of 21units per mg of protein. 2. Direct enzyme linked immunoadsorbent assay (ELISA) method was used to measure the antibody level against HCC GGT. An antibody titration of the blood sample taken from the mouse immunized with the above enzyme showed value over 1:5, 120. 3. The cell fusion of the mouse myeloma cell and the spleen cell of the immunized mouse was generated 14 positive wells in 221 wells. (specific fusion efficacy of 6.33%) Only one IgG2a monoclonal antibody was determined by both repetitive cloning procedures and immunoglobulin subisotyplng. 4. A antigen-antibody cross matching after the western blot revealed a specific reaction between the HCC GGT antigen at the 40Kd band and the monoclonal antibody of the hybridoma. And the monoclonal antibody did not react with GGT derived from normal liver or kidney.

米麥混食이 白鼠體內 脂質代謝에 미치는 影響

洪進敎,柳總根 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.3

The comparative study was conducted on the effect of rice diet and rice mixed with barley(10%, 30%, 60%) diet on accumulation of adipose tissue and levels of triglyceride(TG), phospholipid(PL), total cholesterol(TC), and high density lipoprotein(HDL)-cholesterol in serum of albino rats(Wistar). 1. Adipose tissue accumulation and levels of TG, PL, TC serum in were lower in rice mixed with barley diet group than in rice diet group to the extent that the more barley was mixed with rice, the lower the levels of the above parameters. 2. HDL-cholesterol level was lower in the group where more barley was mixed with rice at 2 weeks after feeding. Rice diet group showed the highest level. 3. TC-HDL cholesterol level was lower in rice mixed with barley diet group than in rice diet group. 4. HDL-cholesterol level decreased in all the diet groups as the feeding period went, in contrast that TC-HDL cholesterol level increased gradually during the same period. 5. The average contents of NDF, ADF and lignin in barley(38% polished Convered barley) were 5.25g%, 2.078% and 1.11g%, respectivity. Based on the above observations it can be deduced that rice mixed with barley diet has the effect to suppress the adipose tissue accumulation and serum elevation of lipid level mainly by dietary fibers contained in barley to the extent that the more barley contained in diet, the more inhbitory effect it shows.

γ-Glutamyltranspeptidase가 肝細胞增殖에 미치는 影響

朴鈗圭,柳總根 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.1

This study was intended to examine the role of γ-Glutamyltranspeptidase (γ-GT) in controlling hepatocyte proliferation and the mechanism of the enzyme action by which the enzyme manifests such effect. Adult rat hepatocytes were isolated and cultured to observe normal growth pattern. and the effect of inhibitor (serine-borate complex) of γ-GT upon hepatocyte proliferation, and to decide the concentration of enzyme inhibitor at which hepatocyte proliferation is inhibited. In addition. Changes of γ-GT activity and DNA synthesis were studied in the absence or presence of enzyme inhibitor employing primary culture of hepatocytes. The following results were obtained 1. Low initial cell density (5-6×10^(5)' cells/3S mm dish) and addition of insulin and EGF prompted hepatocyte proliferation. Whereby, Cell number increased up to 1.5 fold after 32 hours in comparison with initial cell number. 2. The concentration range of enzyme inhibitor in which hepatocyte proliferation was inhibited was more than 1 mM 3. During primary culture of hepatocytes, γ-GT activity showed rapid increase in lag phase on the basis of "zero" time and maximal increase in exponential growing phase. The increasing rate of γ-GT was weakened in stationary phase. 4. During primary culture of hepatocytes. The extent of DNA synthesis showed rapid increase in lag phase on the basis of ''zero" time and gradual increase in exponential growing phase and stationary phase. But. Such increase of DNA synthesis was lost by administration of enzyme inhibitor. The above results suggest that action and increased activity of γ-GT is necessary for increase of DNA synthesis in hepatocyte proliferation.

臟器內 γ-Glutamyltransferase Isozyme의 免疫學的 考察

朴玧圭,柳總根 최신의학사 1989 最新醫學 Vol.32 No.2