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박세훈,최재도,강주일,최상윤,황순봉,김정수,안병윤 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1
The interferon-induced, double-stranded RNA (dsRNA)- dependent protein kinase PKR plays a key role in interferon- mediated host defense against viral infection, and is implicated in cellular transformation and apoptosis. We have isolated a cDNA of simian PKR encoding a product with 83% amino acid identity to the human homolog and showed that PKR expression is significantly attenuated in the Vero E6 African green monkey kidney cells devoid of type I interferon genes. A variant form of PKR lacking the exon 12 in the kinase domain is produced in these cells, presumably from an alternatively spliced transcript. Unlike wild type PKR, the variant protein named PKR-ΔE12 is incapable of auto-phosphorylation and phosphorylation of eIF2-α, indicating that the kinase sub-domains III and IV embedded in exon 12 are indispensable for catalytic function. PKR-ΔE12 had no dominant negative effect but was weakly phosphorylated in trans by wild type PKR.
Expression of hepatitis C virus nonstructural 4B in transgenic mice
Ai-Guo Wang,문형배,김진만,황순봉,유대열,이동석 생화학분자생물학회 2006 Experimental and molecular medicine Vol.38 No.3
Hepatitis C virus (HCV) is a pathogen that is of great cirrhosis, and hepatocellular carcinoma worldwide. Although the HCV proteins have been intensively investigated over the past decade, the biochemical functions of the NS4B protein are still largely unknown. To investigate NS4B as a potential cau-sative agent of liver disease, transgenic mice expressing the NS4B protein in liver tissue were produced. The transgenic animals were phenoty-pically similar to their normal littermates for up to 18 months of age. Our results suggest that the HCV NS4B protein is not directly cytopathic or oncogenic in our transgenic mice model.