MicroRNA-146a Suppresses Metastatic Activity in Brain Metastasis

황수진,김현호,Ho Jun Seol,박영미,Kang Ho Kim,Myriam Gorospe,남도현 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.3

Primary lung tumors, breast tumors, and melanoma me-tastasize mainly in the brain where therapy is limited to surgery and radiation. To investigate the molecular basis of brain metastases, we isolated brain-trophic metastatic MDA-MB-435-LvBr2 (LvBr2) cells via left ventricle (LV) injection of MDA-MB-435 cells into immunodeficiency (NOD/ SCID) mice. Whereas parent MDA-MB-435 cells displayed an elongated morphology, LvBr2 cells were round and displayed an aggregated distribution. LvBr2 cells expressed lower beta-catenin levels and higher heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNPC) levels than parental cells. Since microRNAs are known to play an important role in cancer progression including metastasis, we screened microRNAs expressed specifically in brain metastases. MicroRNA-146a was almost undetectable in LvBr2 cells and highly expressed in the parental cells. Overexpression of miR-146a increased beta-catenin expression and suppressed the migratory and invasive activity of LvBr2 cells. The miR-146a-elicited decrease in hnRNPC in turn lowered the expression of MMP-1, uPA, and uPAR and inhibited the migratory and invasive activity of LvBr2 cells. Taken together, our findings indicate that miR-146a is virtually absent from brain metastases and can suppress their metastatic potential including their migratory and invasive activities associated with upregulation of beta-catenin and downregulation of hnRNPC.

Dexamethasone으로 유도한 근감소 동물모델에서 상황버섯-오미자박 고상발효 열수추출물의 근감소 개선에 대한 효과

황수진,김영숙,오태우 대한한의학방제학회 2022 大韓韓醫學方劑學會誌 Vol.30 No.4

Objectives : In this study, it was investigated the effects of solid-phase fermentation extraction with Phellinus linteus of discarded Schisandra chinensis extract (PS) and its action mechanism on dexamethasone-induced muscle atrophy in mice. Methods : In mice, muscle atrophy model was induced by dexamethasone (5 mg/kg, I.p) once daily for 2 weeks and with PS extract administration (100 and 300 mg/kg, p.o.) as treatment groups. The changes in body weights, grip strength, Treadmill test, muscle weights, and the expression of atrophy-related genes were measured in muscle atrophy mice. The histological changes of gastrocnemius tissues were also observed by H&E staining with measurement of myofiber size. Results : The administration of PS extract increased significantly body weights, grip strength, treadmill test and muscle weights in muscle atrophy mice. PS extract administration increased significantly the area of myofibers and inhibited structural damages of muscle and increased significantly the expression of myogenin and decreased significantly the expression of MuRF1, Atrogin1 and phosphorylation of AMPK and PGC1α in muscle tissues of muscle atrophy mice. Conclusions : These results indicate that PS extract has a improvement effects on muscle atrophy with stimulation of myogenic differentiation and inhibition of mRNA degradation that could be related with the activation of AMPK and PGC1α signaling pathways in muscle. This suggests that PS extract can apply to treat muscle atrophy in clinics.

뇌성마비 아동의 재활치료에 있어서 부모 참여에 대한 고찰

황수진,하승희 한국장애인재활협회 2013 재활복지 Vol.17 No.3

발달장애아동의 재활치료에 있어서 치료실과 가정과의 관계는 매우 중요하다. 특히 뇌성마비와 같은 만성적인 장애로 신체적인 운동장애뿐만 아니라 감각장애, 언어장애 등이 복합적으로 나타나는 중복장애의 경우 가정에서의 부모의 역할이 더욱 강조되어야 한다. 따라서 본 연구는 뇌성마비 아동의 재활치료에 있어서 부모 참여에 대한 지원 방안을 살펴보기 위해 뇌성마비 아동 어머니 4명과 재활치료 전문가를 3명을 대상으로 재활치료와 부모교육 프로그램의 경험과 개선사항 등을 주제로 심층면접을 실시하였다. 질적 분석결과 2개의 범주에서 총 8개의 하위범주가 도출되었다: (1)가족치료를 통한 부모 참여: 가정에서의 지속적인 연습, 전문가와 상담, 부모교육 프로그램 참여, 가족치료의 어려움, (2)장애아동 부모단체 활동을 통한 부모 참여: 정서적지지, 정보 공유, 지역사회와의 협력, 장애아동 부모단체 활동의 어려움. 재활치료에 있어서 효과적으로 부모 참여가 이루어지기 위해서는 가족치료를 위한 전문가들의 지지와 장애아동 부모단체를 통한 통합적인 사회적인 지지가 제안되었다. It is important to build a relationship between parents and therapists in rehabilitation treatments. Especially the importance of parents’role at home should be emphasized for effective rehabilitation treatment for children with cerebral palsy as a multiple-handicapped children that had a physical disorders as well as disturbance of sense, and speech-language disorders. The purpose of this study was to develop a support of parents involvement that helps the children with cerebral palsy's rehabilitation treatment. 4 mothers of children with cerebral palsy and 3 rehabilitation therapists participated in the focus group to share their experiences and opinions. Two primary themes and eight sub-themes emerged from qualitative analysis: (1) Parent involvement through family therapy: continuous practice at home, professionals counseling, parent training programs, and the difficulties of family therapy, (2) parents involvement through parents' association with the disabled: emotional support, information sharing, collaboration with local communities, and the difficulties of parents' association with the disabled. We suggest that parents need a professional supports from rehabilitation specialists and social supports by handicapped children’s parents association for effective parents involvement.

디지털기기의 사용이 대학생의 눈과 근육뼈대계에 미치는 영향

황수진,김효진 대한시과학회 2022 대한시과학회지 Vol.24 No.4

Purpose : The purpose of this study was to evaluate the effect of the use of digital devices, including smartphone, on eyes and musculoskeletal system (MS) among university students in the era of COVID-19. Methods : Data obtained from 156 university students were analyzed with an online survey in November 2022. The questionnaire consisted of 16 questions related to computer vision syndrome (CVS), and 11 questions related to the MS, including the general characteristics of the subjects. CVS was evaluated on a frequency of 0~2 points and intensity at 1~2 points, and MS pain was evaluated on a 3-point scale by dividing it into mild, moderate and severe symptoms. Results : The average daily use of digital devices was 7.97±3.46 hours, with laptops (47.4%), tablets (33.3%), desktops (12.2%) and smartphones (7.1%) for online classes. Regular daily exercise was 22.4% of the participants. Symptoms associated with CVS scored the highest for eye dryness, while neck pain was the most severe for the MS. As the time spent using digital devices increased, the pain in the trunk (neck, back and lower back) and the total MS score became more severe, and when taking online classes using a laptop or smartphone, the upper extremity pain score was higher compared to using a desktop. Regular daily exercise was associated with less pain in CVS and MS pain than when not doing it. Conclusion : Prolonged use of digital devices and the type of devices used during online classes affected eyes and musculoskeletal pain. The results of this study showed that convergent research and management of the eye and MS are needed together in the future. 목적: 코로나19의 상황에서 대학생을 대상으로 스마트폰을 포함한 디지털기기의 사용이 눈과 근육뼈대계 통증에미치는 영향을 알아보고자 하였다. 방법: 2022년 11월 온라인 설문조사에 응답한 대학생 156명의 자료를 분석하였다. 설문은 대상자의 일반적 특성을포함하여 코로나19 대유행 동안 디지털기기의 사용시간과 종류, 컴퓨터시각증후군(computer vision syndrome, CVS) 16항목, 그리고 근육뼈대계 관련 11문항으로 구성하였다. CVS의 빈도는 0~2점, 강도는 1~2점으로 평가하였고, 근육뼈대계 통증은 약한 증상, 경도, 그리고 심한 증상으로 구분하여 3점 척도로 평가하여 총점을 산출하였다. 결과: 일일 평균 디지털기기의 사용은 7.97±3.46시간으로 온라인 수업을 위해 노트북(47.4%), 태블릿(33.3%), 데스크톱(12.2%), 그리고 스마트폰(7.1%)을 사용하였다. 매일 규칙적으로 운동하는 경우는 대상자의 22.4% 이었다. 컴퓨터시각증후군 관련 증상은 건조감이 가장 높은 점수를 보였고, 근육뼈대계 항목은 목 통증이 가장 심했다. 디지털기기의 사용시간이 증가할수록 몸통(목, 등, 허리)과 전체 근육뼈대 통증이 심해졌고, 노트북이나 스마트폰을 이용해서온라인 수업을 듣는 경우에는 데스크톱을 사용하는 경우와 비교해 상지통증 점수가 높게 나타났다. 매일 규칙적인운동을 하는 경우에는 하지 않는 경우에 비해 컴퓨터시각증후군과 근육뼈대 통증의 통증이 작았다. 결론 : 디지털기기의 장시간 사용과 온라인 수업 동안에 사용하는 기기의 종류는 눈과 근육뼈대계 통증에 영향을 미쳤다. 본 연구결과를 통해 이러한 환경에서 향후 눈과 근육뼈대계의 융합적인 연구와 관리가 함께 필요함을알 수 있었다.

대학 행정서비스 개선을 위한 행정직원 서비스 실패와 회복에 관한 연구 – 수요자 관점에서 -Ⅱ

황수진,현승용 한국생산관리학회 2015 한국생산관리학회 학술대회 논문집 Vol.2015 No.-

생간건비탕(生肝健脾湯)이 알코올 투여로 유발된 흰쥐의 고지혈증과 간 손상의 예방에 미치는 영향

황수진,최홍식,김승모,우창훈,Hwang, Soo-Jin,Choi, Hong-Sik,Kim, Seung-Mo,Woo, Chang-Hoon 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4

Objectives : This study was performed to investigate the effect of water extracts from Saengkankeonbi-tang on prevention of hyperlipidemia and liver damage induced by alcohol. Methods : Except for the normal group, we fed rats the control and Saengkankeonbi-tang groups with 25% alcohol for 55 days. For the same period we fed the Saengkankeonbi-tang group with Saengkankeonbi-tang extract as well and rats in normal and contrlo group on saline solution. We measured the serum components in rat's blood, body weight and weight of liver. Results : 1. At first, we observed effects of Saengkankeonbi-tang on prevention of hyperlipemia induced by alcohol. Saengkankeonbi-tang group showed significant decrease in total cholesterol levels in comparison with those of the control group. Saengkankeonbi-tang group showed significant increase in HDL-cholesterol levels in comparison with those of the control group. Saengkankeonbi-tang group showed significant increase of body weight in comparison with those of the control group in 4weeks and 8weeks. 2. At second, we observed effects of Saengkankeonbi-tang on prevention of liver damage induced by alcohol. Saengkankeonbi-tang group showed significant decrease in GOT, GPT, LDH and ALP levels in comparison with those of the control group. Saengkankeonbi-tang group showed significant increase of liver weight in comparison with those of the control group. Conclusions : Reviewing these experimental results, it appears that water extracts from Saengkankeonbi-tang have pharmaceutical efficacy on prevention of hyperlipidemia and liver damage induced by alcohol. Therefore further additional study should be conducted to elucidate in depth the pharmaceutical efficacy of these.

학교 다니는 내내 저는 늘 왕따였어요

황수진 한국청소년상담원 2011 상담사례연구집 Vol.2011 No.-

Risk of falls in community-dwelling older adults aged 65 or over with type 2 diabetes mellitus: a systematic review

황수진 물리치료재활과학회 2018 Physical therapy rehabilitation science Vol.7 No.3

Objective: Older persons with diabetes mellitus (DM) are particularly more likely to have fallen in the previous year than those without DM. The purpose of this study was to investigate the relationship between the risk of falls and type 2 DM in older adults who are 65 years of age or above. Design: A systematic review. Methods: PubMed and other two databases were searched up to August 2, 2018. Observational and cohort studies evaluating fall risk in people who are 65 years of age or above with DM were included. This review extracted the following information from each study selected: first author’s surname, year of publication, country, average follow-up period, sex, age at enrollment, study population, measurement variables, relative risk, 95% confidence intervals and controlled variables. Results: This review involved nine cohort studies with 3,765 older adults with DM and 12,989 older adults without DM. Six studies compared with or without DM and two studies compared fallers with non-fallers with DM. Risk factors for falls included impaired cognitive function, diabetes-related complications (peripheral nerve dysfunction, visual impairment), and physical function (balance, gait velocity, muscle strength, and severity of physical activities). Conclusions: People who are 65 years of age or above with DM have increased risk of falling caused by impaired cognitive function, peripheral nerve dysfunction, visual impairment, and physical function in community-dwellers. For adults who are 65 years of age or older with DM, research fields and clinical settings should consider therapeutic approaches to improve these risk factors for falls.

웃음의 건강학

황수진,Hwang, Su-Jin 기술표준원 2005 기술표준 Vol.38 No.-

자기애와 학교폭력 가해경험 간의 관계: 자존감, 자존감 안정성의 조절효과

황수진,김현주 미래를 여는 청소년학회 2016 미래청소년학회지 Vol.13 No.2

본 연구의 목적은 자기애가 학교폭력 가해경험에 미치는 영향을 확인하고, 이들의 관계에서 자존감과 자존감 안정성의 조절효과를 검증하는 것이다. 연구 대상은 중학교와 고등학교에 재학중인 학생이며, 자기애(자기주도적, 타인의존적)와 자존감, 자존감 안정성 수준 및 학교폭력 가해경험을 설문조사를 통해 자료를 수집하였다. 자료 분석은 SPSS 21.0 통계 프로그램을 이용하였다. 연구의 주요 결과는 다음과 같다. 첫째, 타인의존적 자기애가 학교폭력 가해경험에 정적으로 유의한 영향을 미치는 것으로 나타났다. 둘째, 자존감과 자존감 안정성은 자기애가 학교폭력 가해경험에 미치는 영향을 조절하였으며, 자존감과 자존감 안정성이 자기애의 하위요인에 따라 학교폭력 가해경험에 미치는 영향이 상이하였다. 특히 자존감이 높고 자존감 안정성이 모두 높은 경우에, 타인의존적 자기애의 증가는 학교폭력 가해경험을 증가시키는 반면, 자기주도적 자기애의 증가는 학교폭력 가해경험을 낮추는 역할을 하였다. 또한 자기주도적 자기애의 경우에 자존감과 자존감 안정성의 완화효과가 크지만, 타인의존적 자기애의 경우에는 자존감과 자존감 안정성의 조절효과보다 타인의존적 자기애가 학교폭력 가해경험에 미치는 부적효과가 더 크다는 것을 발견하였다. The purpose of this study is to identify the effects a student's narcissism has on perpetrating school violence, and to test the moderating effects of self-esteem and the stability of self-esteem within that relationship. The subjects of this study are middle school and high school students, and the data has been collected through questionnaires regarding two dimensional narcissism (self-initiative and other-dependent), self-esteem, self-esteem stability and experiences on school violence. SPSS 21.0 version was used for statistical analysis of data. The main results are the following. First, the other-dependent form of narcissism has shown to have significant influence on the perpetration of school violence. Second, self-esteem and self-esteem stability have moderating effects on the perpetration of school violence, but the moderating effects of self-esteem and self-esteem stability differ according to the sub-factors of narcissism. Especially, in case of high self-esteem and high self-esteem stability, other-dependent narcissism increased the school violence but self-initiative narcissism decreased school violence. Also in case of self-initiative narcissism, self-esteem and self-esteem stability had a greater buffering effect, while other-dependent narcissism had a greater negative effects on school violence than the moderating effects of self-esteem and self-esteem stability had on school violence.