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랫트에서 2,3,7,8-Tetrachlorodibenzo-p-dioxin(TCDD) 유발 생체 독성에 대한 조직배양 산삼부정근 사포닌의 치유효과
황석연,박선우,박정숙,한건,Hwang, Seock-Yeon,Park, Sun-Woo,Park, Jeong-Sook,Han, Kun 대한약학회 2006 약학회지 Vol.50 No.4
The therapeutic effect of tissue cultured root of mountain ginseng (Panax ginseng) (tcMG) on 2,3,7,8-tetrachlorodaibenzo-p-dioxin (TCDD) induced toxicity in rat was investigated. The rats were assigned into three groups (10 rats/group), control, TCDD exposed group and tcMG treated group after TCDD exposed. $50\;{\mu}g/kg$ of TCDD was injected by i.p. for TCDD exposed group and 30 mg/kg of tcMG saponin was administered for 4 weeks by oral gavage for tcMG treated group. The weights of body, spleen, kidney, thymus, testes and epididymides were decreased in the single TCDD treatment. However these organs was significantly recovered by tcMG saponin except thymus (p<0.05). tcMG decreased the level of hepatic demage maker enzymes, AST and ALP. It also lowered total cholesterol and triglyceride. The level of serum triglyceride was significantly decreased in tcMG saponin treated group compared with the control. Histopathological examination revealed morphological change in the liver spleen, thymus and testes of TCDD treated rats. However they were relatively well preserved in the tcMG treatment group. In conclusion, TCDD induced toxicity was some repaired by tcMG. tcMG may be useful for prevention and treatment of TCDD induced toxicity.
기니피그에서 홍삼 사포닌의 2,3,7,8-TCDD 독성 방어 효과
황석연,이찬용,Hwang, Seock-Yeon,Lee, Chan-Yong 한국환경보건학회 2009 한국환경보건학회지 Vol.35 No.4
This study was carried out to investigate the protective effect of Red Ginseng Saponins on 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induced toxicities in guinea pigs ($200{\pm}10$ g). Normal control (NC) group guinea pigs ($200{\pm}10$ g) received vehicle and saline, while the TCDD-treated (TT) group was given water-extract (WE), saponin fraction (SF) and non-saponin fraction (NSF). Korean red ginseng fractions were administered from 1 week before TCDD-exposure for 4 weeks. Body weight loss and deteriorated clinical parameters related to sugar metabolism and liver function such as lipase and AST, respectively, these were significantly reduced by both saponin and non-saponin fractions. However, increase of lipase was attenuated by the saponin fraction in a dose-dependent manner. Only AST was affected by the saponin fraction. The results suggest that saponins are active substances in the Korean red ginseng water extract against TCDD induced toxicities in Guinea pigs.
최근 충북지역에서 분리된 Gram 양성 세균종과 항생물질의 감수성 양상
황석연(Seock-Yeon Hwang),최원창(Won-Chang Choi) 대한의생명과학회 1999 Biomedical Science Letters Vol.5 No.2
임상검체에서 분리되는 병원균을 주기적으로 분석하고 이에 대한 항균제 감수성의 변화 양상을 파악하는 것은 임상에서의 적절한 항균제의 선택이나 내성균의 감독과 조절을 위해 필수적이라 할 수 있다. 저자들은 충북대학병원에 1996년 1년간 내원 환자의 미생물 배양 검사에서 분리, 동정된 1,689 균주중 Gram 양성인 542 균주의 분리 빈도 및 주요 균종에 대하여 항생제 감수성 양상을 조사하였다. Gram 양성 균종은 S. aureus, S. pneumoniae, S. epidermidis, S. hemolyticus, coagulase negative Staphylococcus (CNS), Enterococcus faecalis 순으로 분리되었다. 한편, 항생제 감수성 시험 결과 S. aureus의 항생제 감수성은 penicillin과 gentamicin에 대한 저항성이 현저히 높았으며, teicoplanin과 vancomycin (MIC?? 0.5㎍/㎖)에 감수성이 높은 것으로 나타났다. CNS의 경우도 S. aureus와 대동소이한 결과를 보였으나, S. epidermidis의 경우에는 ciprofloxacin과 clindamycin에 감수성이 높은 것으로 나타났다. Enterococcus spp.는 vancomycin, penicillin, tetracycline 등에 저항성이 매우 높았으며 , 검사한 모든 항생제에 대하여 대부분의 감수성이 zig-zag pattern을 보였다. In order to control resistant strains and to properly select the antimicrobial agents, it is of quite importance to know current trends of bacterial species and changing patterns of antimicrobial resistance rates. The authors studied the results of 542 Gram-positive strains among 1,689 strains isolated at Chung-buk National University Hospital in 1996. The frequently isolated Gram-positive microorganisms were Staphylococcus aureus, Streptococcus pneumoniae, Staphylococcus epidermidis and Enterococcus faecalis in descending order. S. aureus showed high resistance to penicillin, gentamicin, and susceptibility to teicoplanin and vancomycin. Coagulase negative Staphylococcus was highly resistant to all of the antibiotics used in this experiment except teicoplanin and vancomycin. Enterococcus were highly resistant to vancomycin, penicillin and tetracycline. MIC of Gram- positve oaganisms was appeared to be zig-zag pattern.
황석연 ( Seock Yeon Hwang ),김동규 ( Dong Kyu Kim ),손창섭 ( Chang Sub Son ),이남진 ( Nam Jin Lee ),박종배 ( Jong Bae Park ),조정희 ( Jung Hee Cho ),김윤배 ( Yun Bae Kim ),배형준 ( Hyung Joon Bae ) 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.2
The purpose of this study was to investigate the variation in constituent element of blood between the massage group and non-massage group in the period of convalescence after giving intense physical exercise for ten T’ae-Kwondo martial artists. In the variations of red blood cells, white blood cells, hematocrit, hemoglobin and blood platelet, levels decreased during the rest time after physical exercise, but the subjects in the massage group showed lower tendency than the subjects in the non-massage group after physical exercise. The variation of calcium showed an ascension tendency in the massage group after physical exercise. Creatine phosphokinase was increased after physical exercise, but it showed a tendency of rapid decrease in the massage group. Lactic acid showed a variation increase after physical exercise. It showed a tendency of decrease during the rest time in the non-massage group, but it was increased after sports massage. The variation of blood glucose was increased after physical exercise. It was maintained in the massage group after physical exercise in contrast to the non-massage group whose levels decreased during the rest time.
관상동맥질환 환자에 었어서 음주, 홉연 및 운동이 호모시스테인, 엽산 그리고 C-반응성 단백의 레벨에 미치는 영향
황석연 ( Seock Yeon Hwang ),조민자 ( Min Ja Cho ),장철수 ( Cheoul Soo Chang ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2
This study was carried out to investigate the effects of drinking, smoking and exercise on homocysteine, folate and the C reactive protein in patients with coronaη artery disease. A total of 50 subjects (male; 37, female; 13) with coronary artery disease was assigned to patient group. The normal control group consisted of 33 subjects (male; 21, female; 12) without a history of metabolic or cardiovascular disease. Body mass index (BMI), blood pressure, and concentration of bl∞d homocysteine, fl이ate, C-reactive protein (CRP) , and lipids were analyzed to determine the effect of drinking, smoking and exercise on those parameters related to coronary artery disease. Concentrations of CRP (p<0.001), total cholesterol (p<0.001), triglyceride (p<0.OO5), HDLcholesterol (p<0.OO2) and LDL-cholesterol (p<0.04) were significantly higher in the patient group. It was also shown that one of either homocysteine, folate, or CRP levels has a direct relationship with habit. Among the three habits, smoking played the worst role in folate and CRP levels in the patient group. Homocysteine and CRP (p<0.001) concentrations were increased and the folate concentration (p<0.05) was decreased in the smoking group. These results suggest that smoking makes matters of coronary artery disease worse; therefore, it would be critical for patients with coronary 따teη disorder to quit smoking.
황석연(Seock-Yeon Hwang),신지순(Ji Soon Sin),권운(Woon Kwon),채희열(Hee-Youl Chai),조정희(Jung-Hee Cho),이남진(Nam Jin Lee),박종배(Jong Bae Park),김익수(Iksoo Kim),류강선(Kang Sun Ryu),윤치영(Chi-Young Yun),강종구(Jong-Koo Kang),김윤배 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.2
The purpose of this study was to confirm the safety of the extract of cricket, Gryllus bimaculatus. Male Sprague-Dawley rats were divided into 5 groups, 10 animals in each group, and orally administered with the cricket extract at doses of 0 (vehicle), 25, 50, 100 or 200 ㎎/㎏ for 2 weeks. In the results, there were no significant differences in the body weight gain between vehicle control and cricket-treated groups. Also, significant changes in daily food intake and water consumption were not observed through tout the experimental period. In addition, hematological parameters did not show any significant or dose-dependent alterations. Serum biochemical parameters including aspartate transaminase, alkaline phosphatase, creatine kinase, cholesterol, triglyceride, lactate dehydrogenase, pancreatic amylase, iron and magnesium decreased at high doses, in contrast to increases in glucose and potassium levels, although such changes were in normal ranges. In gross findings, no remarkable lesions were seen, except a light increase in only absolute weight, but not relative weight, of submaxillary glands at 25 ㎎/㎏ of cricket. In addition, no abnormal histopathological findings were observed. Taken together, it is suggested that repeated treatment with the extract of cricket may not exert considerable side effects.
황석연(Seock-Yeon Hwang),권운(Woon Kwon),채희열(Hee-Youl Chai),조영민(Young-Min Cho),류재면(Jae Myun Ryu),김동규(Dong Kyu Kim),신지순(Ji Soon Sin),김태명(Tae Kyung Kim),조정희(Jung-Hee Cho),신선희(Sunhee Shin),박정휘(Jung-Hui Park) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.3
Four-week repeated-dose toxicity of Kamiguibitang was investigated in rats. Male Sprague-Dawley rats were orally administered with Kamiguibitang at doses of 200, 800, 1,600 or 3,200 ㎎/㎏/day or its vehicle for 28 days. There were no significant differences in the body weight gain between vehicle control and Kamiguibitang-treated groups. Significant changes in daily feed intake and water consumption were not observed throughout the experimental period. There were trends of increase in platelets and white blood cells, in parallel with increases in serum globulin level and spleen weight, suggestive of inflammatory response and/or immune enhancement. Serum parameters of hepatic and renal injuries, such as aspartate transaminase, alanine transaminase, alkaline phosphatase, cholesterol, triglyceride, creatinine, phosphorus, calcium, sodium and potassium, also increased at low doses (200-800 ㎎/㎏) of Kamiguibitang, although the levels were suppressed at high doses (1,600-3,200 ㎎/㎏). However, no gross and histopathological lesions were seen at all doses. Based on the results, no observed adverse effect level (NOAEL) of Kamiguibitang was found to be lower than 200 ㎎/㎏, which is comparable with clinical dose (100 ㎎/㎏) in human. In spite of the relatively-low NOAEL, it is suggested that repeated treatment with Kamiguibitang may not exert considerable adverse effects, as inferred from that major hematological and blood biochemical changes were results of pharmacological effectiveness on immunomodulation, and that no histopathological lesions were exerted up to 32 folds of clinical dose (3,200 ㎎/㎏),
황석연(Seock-Yeon Hwang),권운(Woon Kwon),채희열(Hee-Youl Chai),조영민(Young-Min Cho),이남진(Nam Jin Lee),류재면(Jae Myun Ryu),신지순(Ji Soon Sin),김태명(Tae Myung Kim),조정희(Jung-Hee Cho),김은주(Eun Ju Kim),박정휘(Jung-Hui Park),강종 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.3
Four-week repeated-dose toxicity study was performed to confirm the safety of the water extract of Mori radicis cortex single (MRCS) or mixed (MRCM) compound. Male Sprague-Dawley rats were orally administered with MRCS or MRCM at doses of 0.5, 1.0 or 2.0 g/㎏/day for 28 days. In the results, there were no significant differences in the body weight gain between vehicle control and MRCS or MRCM treatment groups. Also, no significant changes in daily feed intake and water consumption were observed throughout the experimental period. In hematological analysis, there was a trend of increases in red blood cells at 0.5 and 1.0 g/㎏ of MRCM and in hemoglobin at 0.5 g/㎏ of MRCM, although such changes were in normal ranges. In addition, white blood cells, especially neutrophils, slightly increased, without statistical significance, following treatment with MRCS or MRCM. Interestingly, serum biochemical parameters including aspartate transaminase and alanine transaminase, related to hepatic injuries, decreased at all doses after 28-day treatment with MRCS or MRCM, suggestive of protective effects against tissue damage. No significant changes in organ weights were observed, in accordance with normal features in gross and microscopic findings. Taken together, it is suggested that repeated treatment with the extract of Mori radicis cortex or its mixed compound, available in oriental clinics, may not exert considerable side effects.