종자 저장 단백질 유전인자

홍주봉 ( Choo Bong Hong ) 생화학분자생물학회 1991 생화학총설집 Vol.3 No.-

고등식물 형질 전환용 벡터

홍주봉(Choo Bong Hong),최인성(In Seong Choe) 생화학분자생물학회(구 한국생화학분자생물학회) 1988 생화학총설집 Vol.2 No.-

영양생산단계에 있는 주온성 (走溫性) 돌연변이체

홍주봉 (Choo Bong Hong) 한국식물학회 1983 Journal of Plant Biology Vol.26 No.3

Temperature responses of amoebae of thermotactic mutants have been investigated. Amoebae of the mutant strain HO 428 showed positive thermotaxis which is strong at lower temperatures and drops sharply above the growth temperature of amoebae. The temperature response of HO 428 amoebae was not affected by the length of amoebae on the gradients. HO 596 amebae seemed to have both positive and negative thermotactic responses shortly after food depletion. Longer exposure of these amoebae on the thermal gradients induced a stronger negative response at lower temperatures and and apparent positive response at higher temperatures. A similar change could be observed in HO 1445 amoebae. Based on the steady positive thermotactic response by HO 428 amoebae and the mode of change in temperature response at higher temperatures, 24℃ and 26℃, by HO 596 amoebae, a model for the temperature response of vegetative Dictyostelium discoideum amoebae, strain HL 50, has been proposed. The main features of the model are: a positive response at the thermal gradients with midpoint temperatures lower than the growth temperatures of amoebae and a negative response above it.

간편한 고등식물 (高等植物) RNA 분리 방법

홍주봉(Choo Bong Hong),전재흥(Jae Heung Jeon) 한국식물학회 1987 Journal of Plant Biology Vol.30 No.3

N/A

형질전환된 담배에서 해녀콩 Leghemoglobin cDNA 의 발현

이선영(Seon Young Lee),홍주봉(Choo Bong Hong),안정선(Chung Sun An) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.2

Tobacco (Nicotiana tabacum L. cv. Wisconsin 38) leaf discs were cocultivated with Agrobacterium carrying a leghemoglobin (Lb) cDNA from Canavalia lineata. Seven plants were regenerated from the transformed leaf discs on MS media supplemented with 0.5 ㎎/L BAP, 0.1 ㎎/L α-NAA, 200 ㎎/L kanamycin and 500 ㎎/L carbenicillin. Southern hybridization and PCR of genomic DNA from transgenic plants showed that the Lb cDNA was stably integrated into the genome of the tobacco. Total RNA from the transgenic tobacco showed northern hybridization signal at 1,000 nt and PCR of the first strand cDNA synthesized from the total RNA amplified 0.5 kb Lb cDNA. Furthermore, western hybridization using a polyclonal antibody against soybean Lb showed a 15.8 kD LB-like band on SDS-PAGE of proteins from the transformed tobacco. These results demonstrated that the Lb cDNA of C. lineata was not only incorporated into the genome of tobacco, but also transcribed into mRNA and translated into Lb protein in the transformed tabacco.

Overexpression of NtHSP70-1 Protects Chlorophyll from High Temperature in Plants

Eun Kyung Cho(조은경),Choo Bong Hong(홍주봉) 한국생명과학회 2008 생명과학회지 Vol.18 No.3

고온 단백질 heat shcok protein 70 (HSP70)은 분자샤페론으로써 환경스트레스와 발달단계 동안 단백질을 보호하고 합성하는 다양한 과정에 관여하는 기본적인 단백질이다. 하지만 그 생물학적 기능이 식물에서 아직 정확하게 밝혀지지 않았다. 이에 본 연구에서는 담배에서 고온에 의해 유도된 HSP70인 NtHSP70-1 (AY372069)를 분리하여 그 기능을 연구하였다. NtHSP70-1의 고온 내성 기능을 분석하기 위해 NtHSP70-1이 식물 형질전환용 벡터인 pBKS1-1에 sense 또는 antisense 방향으로 도입되어 형질전환된 식물체와 pBKS1-1만 도입된 형질전환 식물체들을 제조하였다. 형질전환체에 있어서 NtHSP70-1의 발현량은 western blot 분석법을 사용하여 수행하였고 확인된 형질전환체들은 고온 내성 기능분석에 이용되었다. 그 결과 고온 환경에 있어서 NtHSP70-1이 과다발현된 형질전환체들은 그 클로로필의 함량과 생존율이 정상환경 일 때와 유사하였고 반대로 벡터 또는 벡터인 pBKS1-1에 antisense 방향으로 도입되어 형질전환된 식물체들은 클로로필의 파괴로 인한 감소된 생존율을 나타내었다. 고온 처리된 형질전환 식물체에서 클로로필의 함량비교 결과로 NtHSP70-1이 클로로필을 보호함으로써 식물의 고온내성에 기여함을 알 수 있었다. Heat shock protein 70 (HSP70) is known as molecular chaperone, the fundamental protein participating in various processes, from nascent protein synthesis to protection of proteins during abiotic stresses and developmental programs. However, their biological functions in plants are not yet well known. Here, NtHSP70-1 (AY372069), HSP70 of Nicotiana tabacum induced by heat stress was investigated. To analyze the protective role of NtHSP70-1, transgenic tobacco plants, which constitutively overexpressed NtHSP70-1 as well as contained either the vector alone or having NtHSP70-1 in the antisense orientation, were constructed. The altered NtHSP70-1 levels in plants were confirmed by western blotting and transgenic sense lines exhibited tolerance to heat stress. Seedlings with the constitutively expressed NtHSP70-1 grew as green or healthy plants after heat stress. In contrast, transgenic vector or antisense lines exhibited yellowing of leaves or some delay in growth, which finally led to death. Evaluation of chlorophyll contents of heat-shocked transgenic tobacco seedlings indicated that NtHSP70-1 contributes to thermotolerance by preventing chlorophyll synthesis in plants.

고등식물 형질전환용 유전자 운반체 pKCHI 의 개발

정상호(Sang Ho Chung),고경원(Kyung Won Ko),홍주봉(Choo Bong Hong),최인성(In Sung Choi),정태화(Tae Wah Chung) 한국식물학회 1989 Journal of Plant Biology Vol.32 No.1

We have developed a plasmid vector, pKCHl, for the purpose of higher plant transformation. It contains the promoter region of cauliflower mosaic virus 35S transcript (P_35s) and the terminator region of nopaline synthase gene (Tnos) with unique cloning site, Bam HI and Xba Ⅰ, between them. After inserting a foreign gene at the cloning site, P_35s-foreign gene-Tnos cassette can be recovered by using a restriction enzyme Hind Ⅲ.

브로꼴리 엽록체 ATP Synthase Subunit 1 유전자의 5' Exon 부위 cDNA 클론 및 이의 발현 양상

서미정(Mi Chung Suh),이한길(Han Kuil Yi),홍주봉(Choo Bong Hong) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.2

We isolated a cDNA clone, BLSC1, encoding 5` exon of ATP synthase CF_0 subunit I from broccoli. BLSC1 is 285 nucleotides long which consists of a 5` noncoding region of 34 nucleotides, a 5` exon of 145 nucleotides and an intron of 106 nucleotides. The 5` exon codes for 48 amino acids which reveals mostly hydrophobic. The amino acid sequence deduced from BLSC1 shares 83%, 83% and 91% identities with the genes coding for atpF from wheat, rice and spinach, respectively. Genomic Southern blot analysis for BLSC1 showed a typically strong signal for a gene located in the chloroplast genome. Northern blot analysis identified three major classes of transcripts showing strong positive signals in the leaves, but only trace amounts of the transcripts were identified in the other organs like stems, flower buds and roots.

Proteins Heading for the Chloroplast

Hong, Choo Bong 한국식물학회 1990 Journal of Plant Biology Vol.33 No.1

The chloroplast has been the prime light-energy harvesting organelle on earth. It also carries out several key metabolic processes, such as lipid synthesis and nitrogen metabolism. Even though the chloroplast has its own genome, its coding capacity can afford only dozens of proteins, and most of the proteins functioning in the chloroplast are imported from the cytosol where nuclear encoded chlorplast genes are synthesized on free cytosokic ribosomes. Precursor proteins synthesized on cytosolic ribosomes have transit peptides at the amino termini of the proteins, and the transit peptide is sufficient to transfer chloroplast proteins from the cytosol into the chloroplast. When comparing amino acid sequences duduced from the nucleotide sequences of the clones of the chloroplast proteins, high homologies can be found among the transit peptides of proteins with the same function. Overall amino acid compositions of the transit peptides show amphiphilic characters of the transit peptides, and the amphiphilicity indicates that three dimensional structure of the transit peptide is responsible for the translocation of the chloroplast proteins.

Transformation of Tobacco(Nicotiana tabacum) for Hygromycin-Resistance

KIM, Young Soon,SOH, Woong Young,SUH, Mi Chung,HONG, Choo Bong 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Agrobacterium을 사용하여 hygromycin phosphotransferase 유전자를 식물체에 도입하기 위하여 vector를 제조합하였다. 식물발현 vector인 pBKS-1에서 neomycin phosphotransferase유전자를 제거한 다음, CaMV의 35S프로모터와 nopaline synthase의 3' poly A signal 사이에 HPH유전자를 삽입하였다. 이 hygromycin저항성 유전자는 담배 세포에서 선발 marker로서 기능하였다. 비형질 전환체는 저농도의 hygromycin 5 mg/L에서 생장이 저해된 반면, 형질전환체는 hygromycin 50 mg/L를 함유하는 배지상에서 선발되어 완전한 식물체로 분화되었다. Northern hybridization 방법으로 형질전환체에 도입된 HPH유전자의 발현을 확인하였다. we constructed a vector for the integration of a hygromycin phosphotransferase gene into plant genome by Agrobacterium mediated transformation. A bacterial coding sequence for the hygromycin phosphotransferase was inserted into a plant expression vector, pBKS-1, between CaMV 35S promoter and the nopaline synthase 3' polyadenylation signal after deleting the neomycin phosphotransferase gene from pBKS-1. The bacterial resistance gene to hygromycin B was expressed as a dominant selection marker in tobacco cells. Transformants could be selected in 50 mg/L hygromycin while the untransformed cells were nomally sensitive to 5mg/L hygromycin. Northern hybridization confirmed the presence and expression of the hygromycin phosphotransferase gene in the transformed plants.