Functional Analyses of Pepper Chitinase, Pathogenesis-Related Protein 1, Lipase and RING-Finger Protein Genes in Disease Resistance and Abiotic Stress Tolerance

홍점규 고려대학교 2005 고려대학교 박사학위 논문 Vol.0 No.-

Application of Volatile Antifungal Plant Essential Oils for Controlling Pepper Fruit Anthracnose by Colletotrichum gloeosporioides

홍점규,양혜지,정희수,윤동준,상미경,전용철 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.3

Anthracnose caused by Colletotrichum gloeosporioides has been destructive during pepper fruit production in outdoor fields in Korea. In vitro antifungal activities of 15 different plant essential oils or its components were evaluated during conidial germination and mycelial growth of C. gloeosporioides. In vitro conidial germination was most drastically inhibited by vapour treatments with carvacrol, cinnamon oil, trans-cinnamaldehyde, citral, p-cymene and linalool. Inhibition of the mycelial growth by indirect vapour treatment with essential oils was also demonstrated compared with untreated control. Carvacrol, cinnamon oil, trans-cinnamaldehyde, citral and eugenol were among the most inhibitory plant essential oils by the indirect antifungal efficacies. Plant protection efficacies of the plant essential oils were demonstrated by reduced lesion diameter on the C. gloeosporioides-inoculated immature green pepper fruits compared to the inoculated control fruits without any plant essential oil treatment. In planta test showed that all plant essential oils tested in this study demonstrated plant protection efficacies against pepper fruit anthracnose with similar levels. Thus, application of different plant essential oils can be used for ecofriendly disease management of anthracnose during pepper fruit production.

Induction by pathogen, salt and drought of a basic class II chitinase mRNA and its in situ localization in pepper (Capsicum annuum)

홍점규 스칸디나비안 식물생리학회 2002 Physiologia Plantarum Vol.114 No.-

Alternaria Spots in Tomato Leaves Differently Delayed by Four Plant Essential Oil Vapours

홍점규,조연숙,류동현,정지환,권현지,이영희,장석원,박창진 한국식물병리학회 2018 식물병연구 Vol.24 No.4

Alternaria leaf spot disease has been a concern during a tomato production in greenhouse. In vitro antifungal activities of vapours of four plant essential oils, cinnamon oil, fennel oil, origanum oil and thyme oil, were investigated during in vitro conidial germination and mycelial growth of Alternaria alternata causing the tomato leaf spots to find eco-friendly alternatives for chemical fungicides. The four plant essential oils showed different antifungal activities against in vitro conidial germination of A. alternata in dose-dependent manners, and cinnamon oil vapour was most effective to suppress the conidial germination. The four plant essential oils showed similar antifungal activities against the in vitro mycelial growth of A. alternata in dosedependent manners, but low doses of thyme oil vapour slightly increased in vitro mycelial growth of A. alternata. Necrotic lesions on the A. alternata-inoculated tomato leaves were reduced differently depending on kinds and concentrations of plant essential oils. Delayed conidial germination and germ-tube elongation of A. alternata were found on the tomato leaves treated with cinnamon oil and origanum oil vapours at 6 hpi. These results suggest that volatiles from cinnamon oil and origanum oil can be provided as alternatives to manage Alternaria leaf spot during the tomato production eco-friendly.

Differential Control Efficacies of Vitamin Treatments against Bacterial Wilt and Grey Mould Diseases in Tomato Plants

홍점규,김현지,정희수,양혜지,김도훈,성창현,박창진,장석원 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.5

Bacterial wilt and grey mould in tomato plants areeconomically destructive bacterial and fungal diseasescaused by Ralstonia solanacearum and Botrytis cinerea, respectively. Various approaches including chemicaland biological controls have been attempted toarrest the tomato diseases so far. In this study, in vitrogrowths of bacterial R. solanacearum and fungal B. cinerea were evaluated using four different vitaminsincluding thiamine (vitamin B1), niacin (vitamin B3),pyridoxine (vitamin B6), and menadione (vitamin K3). In planta efficacies of the four vitamin treatmentson tomato protection against both diseases were alsodemonstrated. All four vitamins showed different invitro antibacterial activities against R. solanacearumin dose-dependent manners. However, treatment with2 mM thiamine was only effective in reducing bacterialwilt of detached tomato leaves without phytotoxicityunder lower disease pressure (106 colony-formingunit [cfu]/ml). Treatment with the vitamins also differentiallyreduced in vitro conidial germination and mycelialgrowth of B. cinerea . The four vitamins slightlyreduced the conidial germination, and thiamine, pyridoxineand menadione inhibited the mycelial growthof B. cinerea . Menadione began to drastically suppressthe conidial germination and mycelial growth by 5 and0.5 mM, respectively. Grey mould symptoms on theinoculated tomato leaves were significantly reduced bypyridoxine and menadione pretreatments one day priorto the fungal challenge inoculation. These findingssuggest that disease-specific vitamin treatment willbe integrated for eco-friendly management of tomatobacterial wilt and grey mould.

Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants

홍점규,강수란,김연화,윤동준,김도훈,김현지,성창현,강한솔,최창원,김성환,김영식 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.4

Reactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide (H2O2) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion (O2−) and H2O2 was observed and lipid peroxidation also occurred in the tomato leaf tissues. High doses of H2O2 and sodium nitroprusside (SNP)nitric oxide donor showed phytotoxicity to detached tomato leaves 1 day after petiole feeding showing reduced fresh weight. Both H2O2 and SNP have in vitro antibacterial activities against R. solanacearum in a dose-dependent manner, as well as plant protection in detached tomato leaves against bacterial wilt by 106 and 107 cfu/ml of R. solanacearum. H2O2- and SNP-mediated protection was also evaluated in pots using soil-drench treatment with the bacterial inoculation, and relative ‘area under the disease progressive curve (AUDPC)’ was calculated to compare disease protection by H2O2 and/or SNP with untreated control. Neither H2O2 nor SNP protect the tomato seedlings from the bacterial wilt, but H2O2 + SNP mixture significantly decreased disease severity with reduced relative AUDPC. These results suggest that H2O2 and SNP could be used together to control bacterial wilt in tomato plants as bactericidal agents.

Reduced Bacterial Wilt in Tomato Plants by Bactericidal Peroxyacetic Acid Mixture Treatment

홍점규,장수정,이영희,조연숙,윤재길,조혜수,박창진,김효중 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.1

Peroxyacetic acid mixture Perosan, composed of peroxyacetic acid, hydrogen peroxide and acetic acid, was evaluated for eco-friendly management of tomato bacterial wilt by Ralstonia pseudosolanacearum. Perosan drastically suppressed in vitro growth of R. pseudosolanacearum in liquid cultures in dose- and incubation time-dependent manners. Higher perosan doses (0.1 and 1%) caused lowered pH and phytotoxicity to detached leaves of two tomato cultivars Cupirang and Benekia 220 in aqueous solution. Treatment with 0.01% of Perosan delayed wilting symptom significantly in the detached leaves of two cultivars inoculated with R. pseudosolanacearum (107 cfu/ml). Soil drenching of 5% Perosan solution in pots caused severe tissue collapse of tomato seedlings at the four-week-old stage of two tomato cultivars. Treatment with 1% Perosan by soildrenching significantly reduced bacterial wilt in the tomato seedlings of two cultivars. These findings suggest that Perosan treatment can be applied to suppress bacterial wilt during tomato production.

Temporal and subcellular localization of PR-1 proteins in tomato stem tissues infected by virulent and avirulent isolates of Phytophthora capsici

홍점규 Springer-Verlag 2002 Protoplasma Vol.219 No.-

동물성 아미노산 시용이 토양이화학성과 노지고추 생육 및 품질에 미치는 영향

채윤석,홍점규,이상우 한국유기농업학회 2011 韓國有機農業學會誌 Vol.19 No.4

This study was carried out to evaluate the effect of applying levels of Bestamin, animal amino acid, on growth and quality of hot pepper and physicochemical properties of soil. Treatment was given with 200, 400, 800, 1600㎏ per 10a to control of Bestamin, difference of physicochemical properties was lower than Bestamin, and P₂O₅ seems to be reduced, also K⁺, Ca⁺⁺ were obviously low compared to the control. The content of NO₃-N was low compared to control at the Bestamin treated plot. Plant height was longest at 800 treatment and main stem length, main stem weigh and the number of leaves were significantly different with 400 and 800 treatment. No difference was found among the fruit weight, length, diameter in first harvest, but there was significantly different at control of 2nd, 3rd harvest and more increased than 400 and 800 treatment. Fruit weight per plant was the heaviest at 400 and 800 treatment, and the number of fruit was no difference at red pepper but increased with 400 and 800 at green pepper, and yield per l0a was significantly increased to 4503.6㎏ and 4582.5㎏, respectively. Nitrogen in mesophyll accumulation content was obviously reduced at Bestamin treatment compared to control, and amino acid was reduced with control.

아파트 베란다 실내정원에 대한 선호도 분석

신현철,홍점규,최경옥 한국인간·식물·환경학회 2011 인간식물환경학회지 Vol.14 No.6

아파트 베란다를 활용하는 방법으로 실내정원 도입에 대한 선호도 조사 결과 및 도입방안을 제시하면 다음과 같다. 첫째, 아파트베란다에 실내정원을 도입하기 원하는 응답자는 83.7%로 나타났으며 실내정원 도입목적으로 “공기정화”, “취미활동”, “이미지 제고”, “자연에 대한 그리움”, “스트레스 해소”, “자녀들의 자연학습에 도움” 순으로 응답되었다. 둘째, 아파트베란다 실내정원 도입유형은 “휴식형”보다 “감상형”을, 설치유형은 “정원형”을 가장 선호하였다. 셋째. 실내정원의 이미지는 “동양식 풍경”으로 “친근한 이미지”로 조성하는 것을 선호하였다. 넷째, 아파트베란다 실내정원의 도입소재는 “식물”에 대한 선호도가 가장 높았으며, 외래종보다 “자생식물”을, 식물의 종류 중 “관엽식물”을 가장 선호하였다. 또한 플랜터 및 마감재료는 “목재”를 가장 선호하였다. 다섯째, 아파트 베란다 실내정원의 조성비용은 “100만원”을, 관리형식은 “직접관리”를 선호하였으며 연간 위탁관리비용이 “20만원”이면 위탁관리를 선택할 수 있는 것으로 응답되었다. 여섯째, 응답자중 87.8%가 아파트 베란다 실내정원이 삶의 질 향상에 직접적인 영향을 미치는 것에 긍정적으로 응답하였다. 따라서 아파트베란다에 실내정원을 도입하는 것은 공기정화 및 삶의 질 향상을 위해 도입할 필요성이 매우 높으며 목재를 이용하여 플랜터를 만들고 우리나라 자생식물중 관엽식물을 위주로 정원형으로 구성하되 동양식풍경으로 조성하는 것이 가장 효과적인 도입방안인 것으로 조사되었다. This study was performed to figure out the preference for the indoor garden among the using methods of veranda of apartment, and to provide the effective using method of it. As the studying method, the survey was performed, and the survey period was from May 10, 2011 to June 20, 2011. The respondent of the survey was the residents in Gyeongsangnam-do and Jeollanam-do who took the class related to the indoor landscape architecture of veranda of apartment as the education course in the culture center of the department store, and big-size mart, in the university and local government, and in the residents' center of apartment. The first, the respondents wanted to introduce the indoor garden in the veranda of apartment was 83.7%, and the introduction purpose was to “air purification”, “hobby activity”, “improvement of image”, “longing for nature”, “reduction of stress”, and “helping for children's natural studying” by order. The second, the “sentimental type” was preferred in the case of preference for the introduction type, and the “garden type” were preferred in the case of installation type of indoor garden in veranda of apartment. The third, in the case of image of indoor garden, the “oriental scene”, and “intimate image” were preferred. The forth, in the case of the preference for the introduction materials of the indoor garden in veranda of apartment, the preference for “plants” was the highest, and the “wild plants” was preferred more than the introduced species. The preference for “foliage plant” was the highest. Also, in the case of planter and finishing material, the preference for the “wood” was the highest.