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Comparative genomic analysis of mitogen activated protein kinase gene family in grapevine
현태경,김주성,권석윤,김선형 한국유전학회 2010 Genes & Genomics Vol.32 No.3
Mitogen activated protein kinases (MAPKs) are important proteins involved in the signal transduction of extracellular information to intracellular targets, and play a crucial role in the response to biotic and abiotic stresses. Although Arabidopsis MAPKs are used for identification of the putative MAPKs in higher plants, no grapevine MAPK gene nomenclature has yet been appeared in the literature. In this study, we have identified 12 members of grapevine MAPK gene (VvMPK) family via In-silico analysis of current grapevine genome database. The structural comparison of 12 VvMPKs through the analysis of chromosome locations, sequence annotation and paralogous gene pair indicated that VvMPKs have evolved by segmental duplication, rather than by tandem amplification. Although further functional analysis of VvMPKs through in vivo and in vivo experiments will be required, our study provides the basis for future research on the diverse signaling pathways medicated by MAPKs in grapevine.
해당화 열매 성숙단계에 따른 생리활성 및 기능성 물질 변화 분석
현태경,곽민정,엄승희,길진수,김주성 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.3
Fruit ripening is a genetically programmed process involving a number of biochemical and physiological processes assisted by variations in gene expression and enzyme activities. This process generally affects the phytochemical profile and the bioactive principles in fruits and vegetables. To appraise the variation in bioactive principles of fruits from Rosa rugosa during its ripening process, we analyzed the changes in antioxidant and anti-elastase activities and polyphenolic compounds during the four ripening stages of fruits. Overall, an extract of unripe fruits contained the highest levels of total phenolic and flavonoid contents, radical scavenging activity, reducing power, oxygen radical antioxidant capacity, and elastase inhibitory activity, compared with the extracts of fruits at other stages of ripening. Additionally, we found that the reduction of flavonoid content occurs because of decreased transcriptional levels of genes involved in flavonoid biosynthesis pathway during the ripening process. Based on HPLC analysis, we found that the extract of unripe fruits contained the highest amount of myricetin, caffeic acid, chlorogenic acid, syringic acid, and p-coumaric acid and suggested that the antioxidant and anti-elastase activities of the extract obtained from stage 1, should be mediated by the presence of these compounds. Additionally, we analyzed the interaction sites and patterns between these compounds and elastase using the structure-based molecular docking approach, and suggested that chlorogenic acid strongly interacted with elastase. Together, these findings suggest that the maturity of fruits has profound effects on the pharmaceutical value of R. rugosa.
검은콩 발아 과정에 따른 flavonoid 함량 및 flavonoid 생합성 관련 유전자들의 발현 양상 비교 분석
엄승희, 이한용, 현태경 忠北大學校 農業科學硏究所 2016 農業科學硏究 Vol.32 No.2
Plants experience different biochemical and physiological changes during the whole lifecycles. Seed germination means that plants disarm themselves by removing their seedcoat that protects them from pathogens and environmental stress. So, plants have developed a lot of strategies to protect themselves by producing phytochemicals like flavonoids and carotenoids. Black soybean (Glycine max) seeds produce nutritionally important products like essential amino acids, soluble and in soluble carbohydrates and contain a number of minor biochemical having beneficial biological effects like phospholipids, vitamins and minerals, trypsin inhibitors, phytic acid, saponins and isoftavones. Black soybean seed coats have abundant flavonoids like anthocyanins and proanthocyanins and exhibit high antioxidant activities. During the germination process, the total flavonoids were increased and showed high antioxidant activities simultaneously. The seeds have 150mg QE/g of total flavonoids and about 20% antioxidant activities while its sprouts have 350mg QE/g of total flavonoids and about 40% antioxidant activities. In addition we have determined a positive correlation between the expression of flavonoid biosynthesis related genes and flavonoid accumulation during the germination process.
라벤더(Lavandula angustifolia), 생강(Zingiber officinale), 레몬그라스(Cymbopogon citratus) 열수 추출물의 항산화활성
주현주, 진소라, 유동균, 현태경 忠北大學校 農業科學硏究所 2008 農業科學硏究 Vol.36 No.2
Bathing is a means to care for the body in water at an appropriate temperature that provides???? comfort to the body, and it has more efficacy than just cosmetics. Various functions can be given depending on the ingredients. The purpose of this study is to develop bathing aids as strategic products using medicinal herbs and to verify the effect of the bathing aids in vitro. We investigated the contents of flavonoid and phenolic compound, and antioxidant activity of the powdered three plants, extracted with water in 50oC (water extract of Lavandula angustifolia: LA, Zingiber officinale: ZO, Cymbopogon citratus: CC). LA possessed the highest level of both polyphenol (33.24 ± 1.15 μg GAE/mg of D.W) and fla?????? vonoid content (2.62 ± 0.18 μg QE/mg of D.W), while ZO contained the lowest. The antioxidant ?????? activities were analyzed by DPPH free radical scavenging and reducing power assay. The antioxidant activity was measured high in LA and CC, however, ZO exhibited low level of activity in both assays. These data suggest that these Korean herbal bathing candidates may be an effective therapeutic antioxidant supplements.
녹차, 국화, 사철쑥 열수추출물의 항산화 및 주름개선효과
진소라, 주현주, 유동균, 현태경 충북대학교 농업과학기술연구소 2020 農業科學硏究 Vol.36 No.1
This study is to investigate on the antioxidant efficacy of the natural plant extracts, and was intended to confirm the possibility of development of antioxidant and anti-aging as a functional cosmetic material. The hot water extract of Camellia sinensis (CS), Chrysanthemum morifolium (CM) and Artemisia capillaris (AC) were generally measured high in total flavonoid and phenolic content. CM contained the highest level of flavonoid content (6.86 ± 0.12 μg QE/mg of D.W), however CS was the most in phenolic content (39.83 ± 1.12 μg GAE/mg of D.W). The antioxidant activities of three extracts were tested by performing DPPH free radical scavenging and reducing power assay. According to the result of reducing power assay, CS exhibited the highest antioxidant activity, followed by CM and AC in a decreasing order. In case of DPPH free-radical scavenging assay, all three were high, but CS was found to be the most effective. Additionally, CS showed the strongest anti-elastase activity compared to the other extract. Together, these findings suggest