소니아 들로네(Sonia Delaunay)의 의상디자인에 나타난 모더니티(modernity)의 근원에 관한 연구

현선희(Sun Hee Hyun),배수정(Soo Jeong Bae) 한국복식학회 2005 服飾 Vol.55 No.2

The purpose of this study is to find out the origin of perpetuity in the fashion design of Sonia Delaunay, who was recognized as a unique designer in the 20th century. Her characteristics of fashion design appeared as dynamism in the incline of op-art using geometric pattern designed on the basis of abstract painting, color contrast between strong original colors, and repeated geometric patterns. The modern image can be explained as modernity in modern fashion and is continued in these days. The origins of modernity analyzed as follows. First, Sonia Delaunay developed textile design, considering the use of clothing on the basis of scientific idea. Furthermore, she evolved traditional textile design and making process into a practical and rational way through developing new needlework technique. Second, she tried a new style through a straight silhouette in contrast to Art Nouveau style and the test and mixture of different genres. Third, she expressed the simultaneity of Orphism through the parallel structure of various colors. In particular, she used the contrast of strong and clear original colors to express a rhythm of dynamism and give visual interest through color. Finally, Sonia Delaunay attempted to approach the public using clothing, furniture, and curtains with the focus on textiles. It may represent her intention to remove a gap between art and life by understanding the public and their life. As examined above, it can be sad that Sonia Delaunay`s scientific idea with the flow of industrialization, an open attitude not bound by a rule as an artist, an idea of Avant-garde, the comprehension of various colors and the understanding of the public. These factors lie at the bottom of her fashion design.

덩굴차(Gynostemma pentaphyllum Makino) 추출물의 항산화 효과

현선희(Sun Hee Hyun),이종숙(Jong Suk Lee),이광배(Kwang Bae Lee),이재성(Jae Sung Lee) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.4

덩굴차(Gynostemma pentaphyllum Makino)의 물(GPW)과 에탄올 추출물(GPE)의 각 용매 획분에 대한 항산화효과를 검토한 결과는 다음과 같다. 전자공여능은 GPW과 GPE으 water획분을 제외한 나머지 모든 획분에서 모두 90% 이상의 강한 활성을 나타냈으며, SOD 유사활성능은 GPW의 water획분 40%, GPE의 diethyl ether획분 95%의 활성만이 나타났다. Xanthine oxidase 저해 활성은 GPW의 ethyl ether획분, butanol획분, GPE의 diethyl ether획분, buthanol획분에서 80%이상으로 높았으며, 아질산염 소거능은 GPW의 diethyl ether획분과 buthanol획분에서 95%이상, GPE의 diethyl ether획분, chloroform획분, butanol획분이 80%이상으로 높은 것으로 나타났다. 이와 같이 덩굴차 추출물은 항산화 관련 free radical 소거 (electron donating ability, superoxide dismutase acitivity, nitrite scavenging activity)계 및 생성 억제(xanthine oxidase inhibition activity)계에 있어서 분획물에 따라 다소 차이는 있었으나 전자공 여능과 xanthine oxidase 저해 활성은 90%이상의 전반적으로 강한 활성이 있었으며 아질산염 소거능 또한 비극성 및 극성 분획물에서 전반적으로 소거활성이 나타났다. 덩굴차 추출물은 소거계뿐만 아니라 생성 억제계에서도 전반적으로 항산화 활성을 보인 것으로 보아 이것은 덩굴차가 다류로서뿐만 아니라 기능성 식품의 부재료로서의 가능성이 충분히 있는 것으로 판단된다. This study was carried out to examine the antioxidant activity of Gynostemma pentaphyllum Makino. Using the DPPH method, we found that free radical scavenging activity was strong in all the fractions except the water fraction of the water extract (GPW) and ethanol extract (GPE) of G. pentaphyllum Makino. Pseudo-SOD activity was highest in the diethyl ether fraction of the ethanol extract, while the other fractions of the ethanol and water extracts were lower. For xanthine oxidase inhibition activity, the diethyl ether fraction and butanol fraction of GPW, and the diethyl ether fraction and butanol fraction of GPE, showed activities over 80%. Nitrite scavenging ability was strong (over 60%) in all the GPW and GPE fractions. The diethyl ether fraction and butanol fraction of GPW had more effective nitrite scavenging abilities than the other extract fractions. These results suggest that the extracts of G. pentaphyllum Makino can be used as a functional material in a tea or drink.

홍삼의 아미노당(Arginine-Fructose-Glucose) 강화농축액의 면역증진 효과

현선희(Sun Hee Hyun),김영숙(Young Sook Kim),이종원(Jong Won Lee),한창균(Chang-Kyun Han),박민선(Park Min Seon),소승호(Seung-Ho So) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.1

본 실험은 홍삼의 증숙(열처리)과정에서 다량 생성되는 것으로 알려진 아미노당(arginine-fructose-glucose, AFG)을 강화한 농축액의 면역증강 효과를 확인하고자 실시하였다. In vitro에서 정상 마우스의 비장세포를 이용하여 AFG 강화농축액이 비장세포 증식능에 미치는 영향을 알아본 결과, AFG 강화농축액은 비장세포 증식을 증가시키고 LPS와 Con A로 유도되는 비장세포 증식도 유의하게 증가시켰다. Ex vivo에서 수컷 Balb/c 마우스에 AFG 강화농축액을 250, 500, 1,000 mg/kg 용량으로 매일 1회씩 10일 동안 경구투여 하면서 CY로 면역을 저하시킨 후, SRBCs로 감작하여 면역을 유도하여 면역장기인 비장과 흉선의 무게와 항체생성능을 측정하였다. AFG 강화농축액은 CY에 의해 감소하는 비장과 흉선의 무게를 회복하였고, 특히 흉선의 무게를 유의성 있게 증가시켰다. 또한, 비장세포수와 비장세포중의 IgM 항체생성 세포수도 용량 의존적으로 증가하였으며, 특히 500, 1,000 mg/kg 투여군은 현저하게 증가하였다. 이상의 실험 결과 AFG 강화농축액은 비장세포 증식을 촉진하고 CY에 의해 저하된 체액성 면역반응을 활성화시켜 면역기능을 향상시킬 것으로 생각된다. The purpose of this study was to investigate the immuno-modulatory effects of arginine-fructose-glucose (AFG)-enriched extracts of red ginseng. The effect on proliferation in normal mouse splenocytes was measured in vitro. When mouse splenocytes were exposed to various concentrations of AFG-enriched extracts with mitogens (concanavalin A, lipopolysaccharide), splenocyte proliferation significantly increased. In vivo, AFG-enriched extracts were orally administered (250, 500, and 1,000 mg/kg/d for 10 days) with cyclophosphamide (CY, 50 mg/kg, i.p.) to male balb/c mice, which were sensitized with sheep red blood cells (SRBCs) to measure the number of IgM antibody-forming cells in splenocytes. AFG-enriched extracts restored the weights of the spleen and thymus, which were reduced by CY. AFC counts per million splenocytes and total splenocyte number significantly increased in a dose-dependent manner compared with the CY Control group. These results indicate that AFG-enriched extracts increased humoral immunity against CY-induced immunosuppression in animal models.

제주 대정 및 전국 주요 산지별 마늘 추출물의 생리활성

현선희(Sun-Hee Hyun),김미보(Mi-Bo Kim),임상빈(Sang-Bin Lim) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.12

제주 대정 마늘의 특성을 파악하고자, 대정 지역별 마늘과 도외지역 및 중국산 마늘 추출물의 생리활성을 측정하였다. 알리신 함량은 단양 마늘이 457 ㎎/100 g으로 가장 높았으며, 그 다음이 의성(422), 태안(393)과 중국(370), 제주(334)와 남해(328) 마늘 순이었다. 총페놀 함량은 태안 마늘이 3.77 ㎎ GAE/g로 가장 높았고, 남해 마늘이 1.90 ㎎ GAE/g로 가장 낮았다. 제주 대정 마늘의 평균 총페놀 함량은 3.36 ㎎ GAE/g로 전국 주요산지 마늘의 평균 2.87 ㎎ GAE/g에 비하여 높은 함량을 나타내었다. 수용성 항산화능력은 단양(2.88 μmol ascorbic acid equivalents/g of dry extract)과 제주 대정산(평균 2.73)이 가장 높았고, 남해(1.94)와 태안산(1.67)이 가장 낮았다. 지용성 항산화 능력은 남해(5.77 μmol trolox equivalents/g of dry extract), 단양(5.31), 제주 대정산(평균 5.29)이 가장 높았고, 태안산(4.19)이 가장 낮았다. 특히 중국산과 남해산은 지용성 항산화 능력이 각각 6.25와 5.77 μmol/g로 가장 높은 경향을 보여 수용성 항산화 능력이 가장 낮은 것과는 대조를 나타내었다. 전체적으로 지용성 항산화 능력은 수용성 항산화 능력에 비하여 2배 이상 높은 경향을 나타내었다. 통풍저해 활성은 제주대정 마늘(평균 31.1%)이 전국 주요산지 마늘(평균 23.2%) 보다 높은 경향을 나타내었다. 산지별 마늘 추출물의 항균활성은 검정 균주 중에 S. aureus에 대하여 가장 높은 활성을 보였고, 그 다음으로 L. monocytogenes, S. Typhimurium, E. coli 순이었으며, 제주 대정 특히 알뜨르 마늘은 4가지 균주 모두에 대하여 가장 높은 항균 활성을 나타내었다. Physiological activities of the garlic extracts from eight different cultivating areas (three areas from Daejeong Jeju, four major cultivating areas (Danyang, Uiseong, Taean and Namhae) in Korea, and one imported from China) were investigated. Allicin was the highest in the garlic from Danyang (457 ㎎/100 g), and in the decreasing order of Uiseong (422), Taean (393), China (370), Jeju (334), Namhae (328). Total phenolic content (TPC) from Taean was the highest (3.77 ㎎ GAE/g), while that from Namhae was the lowest (1.90). Average TPC (3.36 ㎎ GAE/g) of the garlics from Daejeong Jeju was higher than that (2.87) from major cultivating areas in Korea. The highest integral antioxidative capacities of water-soluble substance were obtained from the garlics of Danyang (3.24 μmol ascorbic acid equivalents/g) and Daejeong Jeju (2.73), while the lowest ones were from Namhae (1.94) and Taean (1.67). The integral antioxidative capacities of lipid-soluble substance were the highest in the garlics from Namhae (5.77 μmol trolox equivalents/g of dry extract), Danyang (5.31) and Daejeong Jeju (5.29), while the lowest was from Taean (4.19). Average xanthine oxidase inhibition activity (31.1%) in the garlics from Daejeong Jeju was higher than that (23.2%) from major cultivating areas in Korea. All of the garlics showed the strong antimicrobial activity against S. aureus, and in the decreasing order of L. monocytogenes, S. Typhimurium, and E. coli. The garlics from Daejeong Jeju (specially from Alttre) showed the strongest antimicrobial activities against all four bacterial strains tested.

제주 자생식물로부터 항산화 및 화장품 기능성 소재 탐색

현선희(Sun-Hee Hyun),정성근(Sung-Keun Jung),죄미경(Mi-Kyung Jwa),송창길(Chang-Khil Song),김지훈(Ji-Hun Kim),임상빈(Sangbin Lim) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.2

제주 자생식물 54종을 대상으로 70% 메탄올로 추출하여, 총페놀 함량, DPPH radical 소거능, xanthine oxidase(XOD), tyrosinase 저해활성을 측정한 후, 생리활성이 높은 5가지 식물을 선정하여 고압 유기용매(100% 메탄올, 13.6㎫, 40℃)로 추출하여 DPPH, tyrosinas, elastase 저해활성을 측정하였다. 70% 메탄올 추출물의 총페놀 함량은 새우나무, 이질풀, 아그배나무, 자금우, 짚신나물이 250㎎ GAE/g 이상으로 높은 함량을 나타내었다. DPPH radical 소거활성은 백량금이 94.1%로 가장 높았다. DPPH radical 소거활성과 총페놀 함량 사이에는 높은 상관관계(R²=0.87)를 나타내었다. Tyrosinase 저해활성은 이삭여뀌, 붉나무, 사방오리나무, 소귀나무가 85% 이상을 나타내었으며, XOD 저해활성은 이삭여뀌와 소귀나무가 90% 이상을 나타내었다. 고압 유기용매 추출물은 70% 메탄올 추출물과 마찬가지로 DPPH 소거능과 tyrosinase 저해활성이 높았다. Tyrosinase와 elastase의 저해활성에 대한 IC??은 자금우가 각각 802와 88 ppm이었고, 소귀나무가 각각 959와 66 ppm이었다. Fifty-four natural plants from Jeju Island, Korea were extracted by 70% methanol. The extracts containing the highest total phenolic contents (TPC) (>250 ㎎ gallic acid equivalents/g dry sample) were obtained from Ostrya japonica, Geranium thunbergii, Malus sieboldii, Ardisia japonica, and Agrimonia pilosa. DPPH inhibition activity was greatest in Ardisia crenata at 94.1%. A high correlation was observed between DPPII inhibition activity and TPC (R²=0.87). Tyrosinase inhibition activities of more than 85% were obtained from the extracts of Persicaria filiformis, Rhus javanica, Alnus firma, and Myrica rubra. On the other hand, the P. filiformis and M. rubra extracts each showed more than 90% XOD inhibition activity. The five natural plants with the highest biological activities were also extracted by pressurized liquid (PLE, 100% methanol, 13.6 ㎫, 40℃). The DPPH and tyrosinase inhibition activities were almost the same in both the 70% methanol and PLE extracts. The IC?? for tyrosinase and elastase inhibition activities in PLE were 802 and 88 ppm in A. japonica, and 959 and 66 ppm in M. rubra, respectively.

홍삼의 분획에 따른 면역활성 비교

현선희(Sun Hee Hyun),김은선(Eun Sun Kim),이상민(Sang Myung Lee),경종수(Eun Sun Kim),이상명(Sang Min Lee),이종원(Jong Won Lee),김미리(Mee Ree Kim),홍진태(Jin Tae Hong),김영숙(Young Sook Kim) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.11

본 연구에서는 홍삼의 면역력 증진에 기여하는 성분을 체계적으로 구명하기 위하여 홍삼분말을 에탄올, 물로 분획하면서 각 분획의 AFCs 형성능 및 Flow cytometery를 이용하여 비장 T 세포수, B 세포수, macrophage 수, CD4<SUP>+</SUP> 및 CD8<SUP>+</SUP> T 세포수를 측정하였다. 6년근 홍삼분말을 100% 에탄올로 추출하여 에탄올 분획과 1차 잔사(R1)를 얻었고, 면역활성이 우수한 1차 잔사(R1)를 물로 추출하여 물분획(W)과 2차 잔사(R2)를 얻었다. 6년근 홍삼분말의 산성 다당체, 진세노사이드 함량은 각각 4.94%, 1.56%이며, 에탄올 분획의 산성 다당체, 진세노사이드 함량은 각각 0.11%, 6.99%, 1차 잔사의 산성 다당체, 진세노사이드 함량은 각각 4.93%, 0.40%였다. 6년근 홍삼분말과 1차 분획에 대한 AFCs 형성능 측정 결과 산성 다당체 함량이 높은 1차 잔사가 가장 우수하였다. 면역활성이 우수한 1차 잔사를 물 추출하여 2차 분획을 얻었으며, 2차 분획의 AFCs 형성능을 측정한 결과 물분획이 가장 우수하였다. 물분획의 산성 다당체, 총 사포닌 함량은 각각 7.46%, 0.61%이며, 다른 분획에 비해 산성 다당체 함량이 가장 높았다. 면역활성이 가장 우수한 물분획을 용량에 따른 비장 세포아형을 분석한 결과 cyclophosphamide 투여에 의해 감소된 비장 총 세포수, T 세포수, B 세포수, macrophage 세포수, CD4<SUP>+</SUP> 및 CD8<SUP>+</SUP> T 세포수가 용량 의존적으로 유의성 있게 증가하였다. 이상의 결과로부터 홍삼의 면역 증진을 나타내는 성분은 사포닌보다는 다당체 함량이 높은 비사포닌계가 활성을 나타낼 것으로 제시되었다. 앞으로 단계별로 분획을 제조하여 면역활성 기능 성분으로서 다당체에 대한 구조를 구명하고 구조에 따른 면역활성 연구가 필요할 것으로 생각된다. The objective of this study was to compare the compositions and immuno-enhancing effects of 6-year-old red ginseng powder (RGP) with those of its fractions. RGP was subjected to extraction with 100% ethanol to obtain an ethanol fraction (E) and residue 1 (R1). Then, R1 was subjected to extraction with distilled water to obtain water fraction (W) and residue 2 (R2). Chemical compositions were as follows: 4.94% acidic polysaccharides and 1.56% ginsenosides (amounts of Rg1, Re, Rf, Rg2, Rb1, Rc, Rd, and Rg3) in RGP, 0.11% acidic polysaccharides and 6.99% ginsenosides in E, 4.93% acidic polysaccharides and 0.40% ginsenosides in R1, 0.50% acidic polysaccharides and 0.30% ginsenosides in R2, and 7.46% acidic polysaccharides and 0.61% ginsenosides in W. Immuno-enhancing effects of fractions from RGP were examined based on suppression of immune responses by cyclophosphamide. In the first fraction test, the antibody response to SRBCs increased significantly in the R1-treated group, but not the E-treated group. In the second fraction test, W showed higher immuno-enhancing effect than R1 and R2. W, which contained the highest amount of acidic polysaccharides, restored numbers of T and B cells, macrophages, as well as CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T cells in the spleen suppressed by cyclophosphamide. These results suggest that acidic polysaccharides from red ginseng may be more effective than saponin in enhancing immune functions and reducing immunotoxicity of cyclophosphamide.

Caenorhabditis elegans를 이용한 phenol류의 독성 연구

정강식,현선희,정세영,Jung Kang-Sik,Hyun Sun-Hee,Choung Se-Young 환경독성보건학회 2006 환경독성보건학회지 Vol.21 No.3

Caenorhabditis elegans(C. elegans) is a free-living soil nematode that commonly used as a biological model and recently, much work has been done using C. elegans as a toxicity model. To evaluate the acute toxicity of phenols to C. elegans, worms were subsequently exposed to nine different xenobiotics. This study described lethal toxicity, reproductive toxicity and movement inhibition using 2-propylphenol, 4-propylphenol, 2-tert-butylphenol, 3-tert-butylphenol, 4-tert-butylphenol, 2-phenylphenol, 4-phenylphenol, nonylphenol and 4-dodecylphenol to C. elegans for 24 hr or 72 hr. We found that phenols used in this study were very toxic to C. elegans. The order of lethal toxicity, reproductive toxicity and movement inhibition is as follows. 4-propylphenol > 2-phenylphenol > 2-tert-butylphenol > 2-propylphenol > nonylphenol > B-tert-butylphenol > 4-dodefylphenol > 4-tert-butylphenol > 4-phenylphenol.

만성적인 에탄올 섭취와 천연물 투여가 흰쥐의 항산화계와 에탄올 산화계에 미치는 영향

김목경,현선희,정세영,Kim, Mok-Kyung,Hyun, Sun-Hee,Choung, Se-Young 대한약학회 2006 약학회지 Vol.50 No.4

This study had been done for the investigation of the effect of Vitis vinifera extract(V), Schisandra chinensis extract (S), Taraxacum officinale extract (T), Gardenia jasminoides extract (G), Angelica acutiloba extract (A) and Paeonia japonica extract (P), and their mixtures on the antioxidant and ethanol oxidation system which was induced by Lieber-DeCarli ethanol liquid diet. Male Sprague-Dawley rats were randomly divided into eight groups: ethanol diet (ED), normal diet (ND), ED+V (100 mg/kg/day), ED+S, ED+T, ED+G, ED+A and ED+P (300 mg/kg/day). We studied the effect on alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) after herbal extracts administration for 6 weeks in rats induced by Lieber-DeCarli ethanol liquid diet. The differences in ADH and ALDH activity of the rats treated with herbal extracts and ED group were not significant. Phase I enzyme activity was found to be significantly higher in the ED+V than the ED group. Phase II enzymes (glutathione-S-transferase, phenol sulfatransferase) activities were found to be higher in the herbal extracts than the ED group. Herbal extracts not only reduced ethanol-induced elevation of level malondialdehyde but also protected against ethanol-induced decrease of reduced glutathione, gluthione reducatse, glutathione peroxidase, catalase and superoxide dismutase activities. Therefore, they can be utilized as a health functional food or new drug candidate for fatty liver and hepatotoxicity which was induced by chronic alcohol consumption.

연구논문 : 생명과학 ; 2-Bromopropane과 1,2-dibromopropane을 마우스에 단희투여시 유발되는 간독성과 면역독성의 특성에 관한 연구

김남희 ( Nam Hee Kim ),현선희 ( Sun Hee Hyun ),김춘화 ( Chun Hua Jin ),이상규 ( Sang Kyu Lee ),이동욱 ( Dong Wook Lee ),전태원 ( Tae Won Jeon ),최창본 ( Chang Bon Choi ),이응석 ( Eung Seok Lee ),최휘건 ( Whi Gun Chae ),정태천 ( Ta 영남대학교 약품개발연구소 2004 영남대학교 약품개발연구소 연구업적집 Vol.14 No.-

랫드 일차 배양 간세포에서 사염화탄소의 독성에 대한 지잠 물추출물의 보호효과

윤수홍,김덕현,현선희,이상규,전태원,정태천,Yun, Soo-Hong,Kim, Duk-Hyun,Hyun, Sun-Hee,Lee, Sang-Kyu,Jeon, Tae-Won,Jeong, Tae-Cheon 대한약학회 2006 약학회지 Vol.50 No.4

Protective effects of the water extract of Protaetia brevitarsis larva against $CCl_4-induced$ toxicity were investigated in primary cultures of adult rat hepatocytes. The extract used in these studies contained several minerals, fatty acids and amino acids. Treatment of hepatocyte cultures with the extract provided a significant protection from the increased LDH activity induced by $CCl_4$. The results demonstrated that the extract may have the protective effect against $CCl_4-induced$ toxicity in hepatocyte cultures.