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일반계 벼의 brassinosteroid 활성물질 검색
현규환,박근형 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.4
Brassinosteroid-like substances in two japonica type Korean ricer were investigated. The extracts from the shoots at the maximum tillering stage were purified by solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography, preparative TLC, Bondesil chromatography and HPLC of normal phase and reverse phase, successively. Biological activity of each purification step were monitored by the rice lamina inclination test. Two cultivars tested in this experiment produced brassinosteroids and endogenous brassinosteroids showing similiarity between two cultivars.
현규환,박근형,김선재 한국농화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.2
In order to explore the brassinosteroid-active component in Cassia tora, methanol extract of immature seeds was purified by sequences of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography and Bondesil chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography. Brassinosteroid substances in separated active fractions were identified as teasterone, castasterone, brassinolide by TLC and HPLC. Our work is probably the first report of endogenous brassinosteroid in Cassia tora. The content of brassinosteroid in Cassia tora as converted into brassinolide was 3.5∼5.5 ng/g fresh weight. The order of brassinosteroid contents was toward to be teasterone, castasterone, brassinolide.
현규환,정현채,김준선 한국자원식물학회 1999 한국자원식물학회지 Vol.12 No.3
전남지방의 고로쇠나무 수액에 대해 sucrose, 무기물, phenol화합물을 분석한 결과는 다음과 같다. 1. Sucrose의 함량은 일반적으로 2차 채취시기에 높게 나타났으며, 지역에 따라 차이는 있으나 2∼3%정도였다. 채취방법은 사구법이 천공법보다 높게 나타났다. 2. ToTal phenol화합물 역시 2차 채취시기에 높게 나타났으며, 0.034∼0.151 mg/100ml의 수준이었고, 가장 다량 함유된 phenol화합물은 phloroglucinol이었으며, 역시 사구법에서 천공법보다 높게 나타났다. 3.무기물의 경우 역시 2차 채취시기에 높게 나타났으며, 역시 사구법에서 천공법보다 높게 나타났다. To find out the temporal and spatial variations in contents of sugar, phenolic compounds and mineral, the sap of Acer mono MAX. were harvested from several regions and periods, and those contents were determined by solvent fractionation, TLC, HPLC and atomic absorption spectrophotometer. Most of all the free sugar contained in the sap of Acer mono MAX. was sucrose, with range of 1.35 to 3.41%. The content of total phenolic compound in the sap of Acer mono MAX. was distributed from 0.034 to 0.151 mg/100m1, and most of phenolic acid was phloroglucinol. The contents of Na, K, Ca and Mg were ranged from 24.11 to 33.15, from 13.75 to 22.10, from 47.19 to 88.00 and from 2.48 to 6.31 ppm, respectively. Contents of sugar, phenolic compounds and mineral decreased sharply at the later harvest. Comparing two different methods of the sap harvest, bore method and groove method, the contents of compounds in the study were determined higher with groove method.
남부지방 서식식물의 유용물질 탐색 II. Saponins과 Alkaloids
현규환,임준택,김학진 한국자원식물학회 1997 한국자원식물학회지 Vol.10 No.4
남부지방에 서식하는 식물체 중에서 꽃기린, 민들레, 박주가리, 후박나무, 팔손이 나무, 달맞이꽃, 동백나무 그리고 유동나무를 대상으로 약용성분의 일부분을 차지하고 있는 saponins 및 alkaloids의 검색을 실시한 결과를 요약하면, 1. Crude saponins 함량 중, crude 인삼 saponins 함량은 달맞이꽃이 가장 높았으며, crude saikosaponins 함량은 박주가리에서 가장 높게 나타났다. 2. Crude alkaloids 함량은 박주가리에서 가장 높게 나타났다. 3. Saponins 획분에 대해 HPLC분석을 실시한 결과, 꽃기린, 민들레 그리고 박주가리에는 인삼 saponin태 물질, 후박나무, 팔손이나무 그리고 달맞이꽃에는 인삼 saponin태 물질과 saikosaponin c태 물질, 동백나무와 유동나무에는 saikosaponin c태 물질의 존재가 추측되었다. 4. Alkaloids 획분에 대해 GC분석을 실시한 결과, 팔손이나무에서만 nicotine태 물질의 존재가 추측되었다. This research was conducted to determine contents of saponins and alkloids for plants distributed in the sourthern region of Korea. The extracts from each plants were purified by solvent fractionation, column chromatography, TLC and analyzed GC and HPLC. As a result, contents crude gingseng saponins and saikosaponins were the highest in Oenothera odorata and Metaplexis japonica, respectively. and contents crude alkaloids was the highest in Metaplexis japonica among the all plants xamined. HPLC was conducted to detect of saponins. As a result, ginseng saponin-like substances was detected in the extracts of Euphobia splendens, Taraxacum mongolicum and Metaplexis japonica, gingsen-like substances and its of saikosaponin c-like substances was detected in the extracts of Camellia japonica and Aleurites fordii. GC was conducted to detect of alkaloid. As a result, nicotine-like substances was detected in only the extracts of Fatsia japonica.
벼의 규산질 비료와 객토 및 철분 시용이 수량과 품질에 미치는 영향
현규환,권병선,신동영,임준택,서영남,정동희 한국자원식물학회 2007 한국자원식물학회지 Vol.20 No.4
- This study was conducted to evaluate the effects of silicate fertilizer, soil addition and iron powder on yieldand quality of rice. High density plot, cropping pattern of rice showed relatively superior values for all the yieldcomponents and yield with 83.2cm in culm length, 21.9cm in panicle length, 8.8 in number of panicles, 65.7% inpercentage of productive tillers, 23.15g in weight of 1,000 grain of brown rice, 658.7kg/10a in rough rice yield and544.7kg/10a in brown rice yield. The results indicate that plot of high density, cropping pattern shows differentadaptabilities to a particular cropping pattern and high density plot seems to be the most suitable culture method of ricefor high yielding.Key words - Rice, High density, Silicate fertilizer, Soil addition, Iron powder, Yielding韓資植誌 Korean J. Plant Res. 20(4):321∼324(2007)으며 실험재료 및 모든 자원은 목포시험장에서 협조를 받았다.실험년도의 기상개황은 평년과 대비하여 보면 평균기온은 연간을 통하여 0.5℃가 낮았고 최고기온은 1.4℃가 낮았으며 최저기온은 0.1℃ 높았고 일조시수는 평년보다 135.8시간이 적었다.강수량은 평년과 같이 1,052㎜ 내외였으며 증발량 역시 평년과비슷한 1,316.2㎜였고 강수일수 역시 평년과 비슷한 95일 이었다. 시럼포장의 기상은 대체로 평년과 별 차이가 없는 기상조건이었다.공시품종으로는 다수확 품종으로 농가에서 선호했던 농림 25호를 공시하였고, 시험전 토양은 Table 1과 같이 양호한 답리작포장이었으며 재배방법은 Table 2와 같이 파종기는 5월 10일,육묘방법은 보통못자리, 파종량은 0.36ℓ/3.3㎡, 이앙시기는 6월 25일, 이앙의 재식밀도는 다수확구(High density)는(30+12)×15㎝, 보통재배구(Spacious planting)는 18×24㎝로하여 다수확구는 3.3㎡당 108주재식, 보통재배구는 75주를 재식하였으며 다수확구(High density)의 시비량과 객토 규산질비료, 철분의 시용은 Table 3과 같이 하였고 보통재배구의 시비량