Application of CRISPR/Cas9‑mediated gene editing for the development of herbicide‑resistant plants

한윤정,김정일 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.5

Herbicide resistance is one of the most important traits for plant biotechnology, which is widely used to improve agricultural efficiency by controlling weeds and also used as a selectable marker during genetic engineering by the transformation. For these reasons, new technologies for conferring herbicide resistance to plants are continuously developing, in which CRISPR/ Cas9-mediated gene editing methods enable precise modifications of DNA sequences and offer a great promise for the improvement of crops. Here, we review recent advances in developing herbicide-resistant plants by the CRISPR/Cas9-mediated gene editing technology, targeting on endogenous genes such as acetolactate synthase (ALS), 5-enolpyruvylshikimate- 3-phosphate synthase (EPSPS), cellulose synthase A catalytic subunit 3 (CESA3), and splicing factor 3B subunit 1 (SF3B1). In addition, we also introduce possible candidate genes to develop herbicide-resistant plants by generating loss-of-function mutations using the CRISPR/Cas9 system.

"급유방(及幼方)" 에 기재된 의안(醫案)에 대한 연구(硏究)

한윤정,장규태,Han, Yun-Jeong,Chang, Gyu-Tae 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.1

Objectives : The Medical Records(醫案) are important materials in studying the developmental process of Korean Traditional Medicine. The purpose of this study was to investigate the medical records which were described in Gupyubang(及幼方), the first Korean book that specialized in pediatrics. Methods : 85 Medical records about medical traits of diagnosis and treatments in Gupyubang were analyzed and those were translated in Korean. Results : Medical records were analyzed as follows; The number of Male was 76 and the number of Female was 9. Prepubertal period was 16 and preschool period 14. Acute febrile convulsion was 6. Measles and blood symptom was 5. Ring worm pain, vomiting & diarrhea, colic pain was 4. Most of them were self treatment except one. 77 cases were improved and 6 cases were dead or worse. The medical records used oral medication, external medication, acupuncture, moxibustion and surgical manners. Conclusion : This study showed that Gupyubang is a experiential book and alse the medical records in Gupyubang was usefulness and had practical value.

메타 계량 활용 한국어 필수 학술기본어휘집 개발 기초 연구

한윤정 한국교양교육학회 2022 교양교육연구 Vol.16 No.1

In this study, a basic discussion was conducted on the development of the “Essential Korean Academic Vocabulary Collection” necessary for teaching and learning major academic vocabulary that foreign students must know for academic performance before entering their major. Since there was no official academic vocabulary list, six appropriate academic vocabulary lists were meta-metered to secure sufficient vocabulary, and the source redundancy and grade information of each vocabulary were scored and evaluated. A total of 4,475 vocabulary words were listed and documented by meta-metering, and the importance was evaluated with 1,154 vocabulary roots excluding 971 sources per person. As a result of the evaluation, 550 of 1,154 roots with 50 or more points were selected as the main heading, and roots with 40 or more points were suggested to be additionally collected as needed if possible. Next, a discussion was conducted on the microstructure setting and technical method of the essential academic vocabulary collection. It was suggested that the microstructure of the collection could be largely composed of a heading presentation part, as well as a word family and usage presentation part. This study is meaningful in that it drew a blueprint for the development of a vocabulary collection that can use academic vocabulary for actual teaching and learning based on existing research for the selection of academic vocabulary. In particular, it is meaningful in that it has dealt with the areas that should be considered when it comes to actual development, and has also increased the possibility of this by analyzing and proposing plans from various angles. 본 연구에서는 외국인 유학생이 전공에 진입하기 전에 학업 수행을 위해 반드시 알아야 할 주요 학술 어휘를 교수⋅학습하는 데 필요한 ‘필수 학술기본어휘집’ 개발의 기초적인 논의를 진행하였다. 공식적인 학술기본어휘 목록이 없어 적절한 학술기본어휘 목록 6개를 메타 계량하여 충분한 어휘량을 확보하고, 출처 중복도, 각 어휘의 등급 정보 등을 점수화하여 평정하는 과정을 거쳤다. 메타 계량으로 총 4,475개의 어휘가 목록화⋅자료화되었으며, 출처 중복도가 1인 971개를 제외하여 1,154개의 어휘 어근을 가지고 중요도를 평정하였다. 평정 결과 1,154개의 어근 중 50점 이상의 550개 어근이 주요 표제어로 선정되었고, 40점 이상의 어휘는 필요에 따라 추가 수록이 가능하도록 제안되었다. 다음으로 필수 학술기본어휘집의 미시구조 설정과 기술 방법 등에 대한 논의를 진행하였다. 어휘집의 미시구조는 크게 표제어 제시부와 관련 어휘 및 용례 제시부로 구성될 수 있음을 제시하였다. 본 연구는 기존에 주로 진행되어 온 학술기본어휘 선정 연구를 기반으로 학술기본어휘를 실제 교수⋅ 학습에 활용할 수 있는 어휘집 개발의 청사진을 그렸다는 점에서 의의가 있다고 본다. 특히 실제 개발에서 고려해야 할 부분을 실질적으로 다루었으며 다각도로 방안을 분석하고 제안하여 가능성을 높였다는 데에도 의의가 있다고 본다.

다의어 학술기본어휘의 전문의미 탐색과 교육적 고찰

한윤정,이은희,한재민 연세대학교 언어연구교육원 한국어학당 2022 외국어로서의 한국어교육 Vol.64 No.-

The purpose of this study is to identify the polysemous characteristics of academic vocabulary, examine its meaning, and derive relevant educational implications. To this end, 232 academic vocabularies were selected based on Hwang Sung-eun(2017), and the vocabulary list including the basic meanings and specialized meanings for each vocabulary was prepared. As a result, it was confirmed that the academic vocabulary with one or more specialized meanings in addition to the basic meanings accounted for a majority: 88%. Since then, an expert investigation was conducted on 163 specialized meanings in seven academic fields with high frequency, and the meaning of 134 final academic vocabularies was analyzed. The analysis confirmed that having a specialized meaning in addition to the basic meaning can be managed with in Korean language education for academic purposes before entering the major. Specifically, the results suggested that if the distance between the basic meaning and the specialized meaning is close, they can be taught and learned simultaneously as per the academic field, if not, caution must be exercised when inferring the specialized meaning from the basic meaning. This study is meaningful in that it has expanded the existing discussion related to academic vocabulary to incorporate a broader perspective. 본 연구의 목적은 학문 목적 한국어교육에서 필수로 다루어지는 학술기본어휘의 다의적 특성을 포착하여 그 의미 양상을 살피고 교육적 시사점을 도출하는 데에 있다. 이를 위하여 황성은(2017)의 어휘 목록을 기반으로 232개의 학술기본어휘를 선별하였고, 어학사전과 백과사전 등을 통해 각 어휘의 기본의미와 전문의미를 자료화하였다. 그 결과 기본의미 외에 전문의미를 하나 이상 갖는 다의어 학술기본어휘가 88%로 많은 수를 차지함을 확인하였다. 이후 빈도수가 높은 7개 학문 분야의 어휘 163개의 전문의미들을 대상으로 전문가 감수를 실시하였고, 이를 바탕으로 최종 정리된 학술기본어휘 134개의 의미 양상을 분석하였다. 분석 결과 기본의미 외에 전문의미를 함께 가지는 경우 전공 진입 전 학문 목적 한국어 교육에서 다룰 수 있음을 확인하였다. 구체적으로 기본의미와 전문의미의 의미 간 거리가 가까운 경우 학문 분야에 따라 두 의미를 함께 교수·학습할 수 있으며, 거리가 먼 경우 기본의미로부터 전문의미를 유추하기 어려우므로 학문 분야별로 주의가 필요함을 제시하였다. 본 연구는 기존의 학술기본어휘 관련 논의를 더 넓은 관점으로 확장하였다는 점에서 그 의의가 있다.

학문 목적 한국어 어휘 진단평가 연구

한윤정 국제한국어교육학회 2021 국제한국어교육학회 춘계학술발표논문집 Vol.2021 No.-

한국어 문법의 발견 학습 교수법을 활용한 쓰기 수업 사례 연구 -고급 학문 목적 학습자를 중심으로-

한윤정 국제한국어교육학회 2019 국제한국어교육학회 춘계학술발표논문집 Vol.2019 No.-

저신장저체중 소아청소년의 모발미네랄분석

한윤정,장규태,이민정,Han, Yun-Jeong,Chang, Gyu-Tae,Lee, Min-Jeong 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.2

Objectives The study was designed to find out the relationships between short stature and low weight among children and teenagers. Methods This research was performed in 21 children and teenagers from August 2006 to June 2007. We measured height, weight, body mass index, obesity rare, bone age, and HTMA. Moreover, we analyzed correlation with height, weight, mineral concentrations and ratios obtained from HTMA. Results There were significant correlations between HTMA and short stature and low weight. 1. Most subjects(76%) had slow metabolic types. 2. Ca, Mg, Zn, Ca/P, and Cr had significant differences between high bone age group and high chronological age group. 3. Fe/Cu had significant differences between a group that recent height is more smaller than inherited height and a group that recent height is more taller than inherited height. 4. Ca, Mg, Ca/P had significant differences between a low weight group and a normal weight group classified by obesity rate. Conclusions Children and teenagers with short stature and low weight have specific mineral characters.

소아(小兒)의 사상체질별(四象體質別) 질병(疾病) 및 증상(症狀) 유형(類型)의 차이

한윤정,김장현,Han, Yun-Jeong,Kim, Jang-Hyun 대한한방소아과학회 2002 대한한방소아과학회지 Vol.16 No.2

We proceeded the judgement of Sasang constitution of 79 children(the age 101 between 15) who have visited Dongguk Bundang Oriental Medical Hospital during 3 months from August 2002 to October 2002 by Questionnaire of Sasang Constitution Classification and conducted questionnaire survey about the type of disease and symptom occurred frequently. The conclusion is following. 1. The distribution of Sasang constitution: Soeum group(少陰人) 32, Taeeuml group(太陰人) 29, Soyang group(少陽人) 18. 2. The distribution of systemic disease occurred frequently : in digestive system, Soeum (46.9%) & Soyang(44.4%) group more than Taeeum group(34.5%) and in genitourinary system and others, Soeum(28.1%) more than Taeeum group(17.2%). 3. The distribution of symptom occurred frequently : in rhinitis and sinusitis, Taeeum(60.0%) & Soeum(60.0%) more than Soyang(45.5%), in abdominal pain, Soeum(58.8%) & Soyang(62.5%) more than Taeeum(36.4%), and in diarrhea, Taeeum(45.5%) more than Soeum(123.5%) & Soyang group(25.0%). Headache & vexation(心煩) are almost found in Soeum group with abdominal pain and excessive sweating is only found in Taeeum group. 4. The efficacy of herbal medication combined with Cervix Cornu Pranum (鹿茸加味劑) : after the medication, change of condition have showed in Taeeum group at the most high rate(31.0%) and showed in Soeum(15.6%) and Soyang(11.1%) group. 5. The distribution of allergic disease: allergic rhinitis and atopic dermatitis took up the majority of all allergic diseases. In morbidity of allergic rhinitis, Taeeum(62.1%) & Soyang(55.6%) more than Soeum(37.5%). In morbidity of other allergic diseases and degree of severity, it has no difference among three Sasang constitution groups.

국내(國內) 시판중(市販中)인 이유식(離乳食)의 실태연구(實態硏究)

한윤정,김장현,Han Yun-Jeong,Kim Jang-Hyun 대한한방소아과학회 1999 대한한방소아과학회지 Vol.13 No.2

A infancy is the period in that growth and development of man are the most fast and neutrial requirement is the most big on his life. The insufficient neutrition of this period can have bad effect uppon man's whole life health not only physically but also pscychogenically and emotionally. Therefore a proper feeding at a proper time is very important to form the good food behavior and emotional development. The rate of using the commercial weaning food in Korea has gradually increased since 1970 and expected to be more increasing in future. In this paper, I investigate the general weaning status, stneutrial component and material content of infant weaning foods marketing in Korea at present. The material for this study have been collected in the exposition of products and data from manufacturing companies and etc. The conclusion is following. 1. The proper weaning period is from $4{\sim}6$ momth to 12 momth, but recently it is inclined to prolong. 2. In weaning methods, using a spoon is superior to using a bottle in food behavior and neutrial status. 3. The commercial weaning foods have two type, the powder type and formula type, but except gober's, all products marketing in Korea are the powder type and a sort of product is very simple. Therefore it is necessary to develp the more various kind of weaning foods, especially the formula type. 4. In the neutrial component, Son-Sik (flour of grain) cannot be up to international standards and the greater parts of weaning foods have too much suger over the standards.

“인생, 야구만큼 모르는 겁니다” - KBS 야구 해설위원 하일성

한윤정 샘터사 2006 월간 샘터 Vol.434 No.-