Diversity of A mating type in Lentinula edodes and mating type preference in the cultivated strains

하병석,김신일,김민석,문윤정,송예린,류재산,류호진,노현수 한국미생물학회 2018 The journal of microbiology Vol.56 No.6

Diversity of A mating type in Lentinula edodes has been assessed by analysis of A mating loci in 127 strains collected from East Asia. It was discovered that hypervariable sequence region with an approximate length of 1 kb in the A mating locus, spanning 5 region of HD2-intergenic region-5 region of HD1, could represent individual A mating type as evidenced by comprehensive mating analysis. The sequence analysis revealed 27 A mating type alleles from 96 cultivated strains and 48 alleles from 31 wild strains. Twelve of them commonly appeared, leaving 63 unique A mating type alleles. It was also revealed that only a few A mating type alleles such as A1, A4, A5, and A7 were prevalent in the cultivated strains, accounting for 62.5% of all A mating types. This implies preferred selection of certain A mating types in the process of strain development and suggests potential role of A mating genes in the expression of genes governing mushroom quality. Dominant expression of an A mating gene HD1 was observed from A1 mating locus, the most prevalent A allele, in A1-containing dikaryons. However, connections between HD1 expression and A1 preference in the cultivated strains remain to be verified. The A mating type was highly diverse in the wild strains. Thirty-six unique A alleles were discovered from relatively small and confined area of mountainous region in Korean peninsula. The number will further increase because no A allele has been recurrently observed in the wild strains and thus newly discovered strain will have good chances to contain new A allele. The high diversity in small area also suggests that the A mating locus has evolved rapidly and thus its diversity will further increase.

국제교육협력을 위한 정보통신윤리교육의 영어 몰입수업 사례연구

하병석 APEC국제교육협력원 2008 국제교육협력연구지 Vol.1 No.2

본 연구의 목적은 정보통신윤리의 영어 몰입수업에 대한 효과, 학습자의 반응 분석, 영어 몰입수엽의 문제점을 찾아 그 대응책을 모색하는 것이다. 연구는질적인 연구의 한 방법인 사례연구법에 의해 실시되었다. 데이터 수집은 연구자의 자기 평가와 학생들의 수엽 만족도 조사표의 응답 결과 그리고 태국 AIV 활동에서 함께 활동하였던 2 명의 교사들 평가를 반영하였다. 수업만족도 조사표로IACE 에서 개발하였으며, 선다형 10문항, 개방형 1 문항으로 작성되었다초등학교 5-6 학년 학생 20 명을 대상으로 시범수업으로 정보통신윤리의 영어몰입수엽 후 학생들의 수엽만족도 조사표에서 영어 몰입수엽은 효과가 있으며 학생들의 반응도 매우 긍정적이었다. 드러난 문제점으로 교사의 학생들 수준에 맞는 의사소통 능력의 부족, 고차원적인 사고 유도의 부족, 수업 중 학생들이 협력할 기회의 제공이 부족, 학생들의 의견 청취와 학생 중심의 수업 부족 등이었다.연구의 결론은 첫째, 정보통신윤리의 영어 몰입수업은 효과가 있었다. 둘째, 학생들의 반응은 매우 긍정적으로 나타났다. 셋째 정보통신윤리의 영어몰입수업에서교사의 철저한 수업계획과 자기 점검이 필요하다는 것이다. The purpose of this study was to examine the effect of English immersion instruction for information ethics, feedback from learners and any possible problems with that instruction, and to explore workable countermeasures. A case study, one of qualitative research methods, was adopted in this study. To gather data, a self-evaluation was conducted by this researcher, and students who received the instruction were asked to write down their satisfaction level with it. And two teachers with whom this researcher worked in Thai-AIV were asked to assess the education as well. A list of questions that lACE developed to evaluate learner satisfaction with instruction was employed, which included 10 questions of multiple choice type and an open-ended question. The subjects in this study were 20 elementary schoolers who were in their fifth and sixth grades. After model lessons were offered by conducting English immersion instruction for information ethics, their satisfaction level with the instruction was investigated, and the English immersion instruction turned out effective. The students responded to that in a very positive way. As for the problems, the teacher who provided that instruction was neither able to communicate with the students in a manner to be appropriate at their level nor to stimulate their higher thinking faculty. He didn't give enough chances for them to team up with, and didn't carefully listen to them, either. Besides, the instruction couldn't be said to be student-centered. The following conclusion was reached in this study: First, English immersion instruction about information ethics was effective. Second, the students responded to that very favorably. Third, teachers should make a thorough preparation for English immersion instruction about information ethics and make a close self-examination.

2016년 구제역 비구조단백질(NSP) 항체 지속 검출농가에서 구제역바이러스 검출을 위한 로프법 적용

하병석,김태성,이진우,이현지,이수미,박혜진,나진주,유소윤,신문균,변재원,박미영,표현미,위성환,남이현,이승윤,구복경 한국동물위생학회 2019 韓國家畜衛生學會誌 Vol.42 No.1

The objective of this study was to assess the possibility of detecting Foot-and-Mouth Disease Virus (FMDV) from the herd-based oral fluids specimens collected by the cotton ropes from pig farms that were found as FMDV nonstructural protein (NSP) antibodies positive. The cotton ropes were applied to detect FMDV in the selected pig farms which NSP antibodies were continuously detected in 2016, including the one pig farm which FMDV antigen were detected at the specimens from the pigsty environment. As the result, FMDV antigen were not detected in the oral fluid specimens collected by the cotton ropes. Theoretically, to detect FMDV antigen from the pigs with NSP antibodies has very low possibility because FMDV antigen disappeared at the time when NSP antibodies were produced by FMDV. Therefore, in order to detect FMDV antigen from the oral fluids using the cotton rope, it would be more effective to be applied to target the FMDV infected pigs rather than the NSP antibodies pos-itive pigs. The collected oral fluids using cotton rope could be useful test specimens to monitor high-density pig populations for FMDV infection. Then, oral fluids sampling using cotton rope will be used for the efficient FMDV surveillance to detect FMDV antigen.

국산 표고(Lentinula edodes) 원목품종의 남부지역 생산성 비교

하병석,반승언,장은경,김경제,서경순,고한규,김선철 한국버섯학회 2016 버섯 Vol.20 No.1

국내에서 육성된 표고 원목품종과 외국산 품종의 생산성 비교를 위해 2014년부터 2016년까지 장흥군 관내 재배농가 3개소를 선정하여 현장시험을 하였다. 시험품종인 국산 품종은 ‘천백고’, ‘수향고’, ‘백화향’, ‘산조 303호’를 사용하였고, 대조품종인 외국산 품종은 남부지역 재배농가에서 많이 재배되고 있는 일본품종인 ‘모 리290’과 ‘모리유지로’를 사용하였다. 2014년 4월에 접종한 후 가을에 조사한 종균 활착력에서는 ‘천백고’ 78.3%, ‘수향고’ 80.0%, ‘백화향’ 66.6%g, ‘산조303호’ 66.5%, ‘모리290’ 60.0%, ‘모리유지로’ 78.3%로 나타나 큰 차이를 보이지 않았다. 그리고 발생 2년차까지 골목 100본당 생산량 조사에서(2015년 가을과 2016년 봄 수확)는 ‘천백고’ 17.7 kg, ‘수향고’ 7.4 kg, ‘백화향’ 14.7 kg, ‘산조303호’ 37.1 kg, ‘모리290’ 27.7 kg, ‘모리유 지로’ 20.1 kg으로 나타났으며, 그중 ‘산조303호’는 외국산 품종과 비교하여 생산량이 뛰어나 경쟁력이 있어 보였다.

국제교육협력을 위한 정보통신윤리교육의 제안 연구 : APECICT 모텔스쿨네트워크의 ICT 활용을 중심으로

하병석 APEC국제교육협력원 2008 국제교육협력연구지 Vol.1 No.1

본 연구의 목적은 APECIMSN의 인터넷의 긍정적 활용을 고찰하고 윤리적 문제점을 탐색하여 그것의 해결 방안을 모색하는 것이다. 먼저 인터넷 긍정적 활용의 사례를 분석하여 그 의미를 찾는다. 인터넷의 비윤리적 활용 사례를 찾아 예방할 수 있는 대책으로써 정보통신윤리교육의 펼요성과 방안을 제안한다.인터넷자료로써 APECIMSN 의 홈페이지 및 영문홈페이지와 IACE 홈페이지를 활용하였다. 인터넷활용 실태를 파악하였으며 긍정적 영향은 미래학교 모형의 탐색 정보화 기술 능력의 향상과 자기주도적 학습능력의 배양 국제적 의사소통 능력의 향상 사회적 기술의 탐색 국제적 감각의 획득과 민간외교역할, 영어 실력의 향상으로 밝혀졌다. 이러한 정보 활용의 의미는 인터넷의 긍정적 활용의 모범이 되며교육을 통한 국제간 원격학습의 가능성 획득, 민간 외교의 실현, 영어 몰입수업이라는 의미가 있다. 문제점은 저작권 위반 이 나타났다. 대응책으로는 정보통신윤리교육를 국제교육협력의 프로젝트로써 진행하되, 교육청에서 지원하는 교육연구회나 교사 동아리 등을 활용하는 방안이 있다. 전문계 고등학교의 경우전문교과 연구회 운영을 국제교육협력활동과 연계하는 방안을 제안한다. The purpose of this study was to delve into the positive utilization of the Internet by APEC IMSN, any ethical problems with it and possible solutions for the problems. lt0 0s first meant to analyze good cases of utilizing the Internet to explore the meaning of the Internet use, and then another cases that employed the net in an unethical manner were investigated to raise awareness about the necessity and method of information ethics education as a way to prevent the unethical use of the net. The Korean and English websites of APEC IMSN and lACE website linked to the sites were utilized in this study. How the organizations took advantage of the Internet was examined, and the Internet use appeared to make it easier to do the followings, which could be called the positive effect of the Internet: exploring models for future school, furthering information literacy, fostering self-directed learning capabilities, improving international communicative competency, inquiring into social skills, acquiring an eye for the world, serving as a private diplomat, and bolstering English skills. The organizations set a good model for the positive utilization of the Internet, which showed the possibility of global distance learning, private diplomacy and English immersion instruction. And there was a problem with it: infringement of copyright. To iron out the problem, information ethics education should be offered as one of cooperative international education projects, and teacher clubs and workshops backed up by local educational authorities could be utilized to help resolve the problem as well. In the event of vocational high schools, business course workshops could be run in association with collaborative international education activities.

原價節減을 위한 PAC技法의 適用

河炳奭 경상대학교 경영행정대학원 1990 最高管理者課程論文集 Vol.1 No.-

논문 : 정보화 역기능에 대한 불교윤리적 관점 연구

하병석 ( Byeong Seok Ha ) 동아시아불교문화학회 2011 동아시아불교문화 Vol.8 No.-

팔정도에 근거한 정보윤리 가능성 연구

하병석 ( Byeong Seok Ha ) 한국윤리교육학회 2011 윤리교육연구 Vol.0 No.25

정보화로 인해 얻는 편리함은 수없이 많지만 이로 인한 역기능의 발생도 많은 윤리적 문제를 야기하고 있다. 그러나 이에 대한 대책들은 아직 미흡한 실정이다. 팔정도는 불교윤리의 가장 포괄적인 윤리체계이므로 이를 활용하면 정보화의 윤리적 문제를 해결할 수 있을 것이다. 팔정도는 정견, 정사유, 정어, 정업, 정정진, 정념, 정정의 여덟 가지 덕목체계가 어우러져 윤리적 기능을 한다. 여기에는 정보화의 윤리적 문제를 해결할 수 있는 내용과 교리가 들어 있다. 정견에서 연기법, 참회, 선인선과악인악과와 같은 교리와 이성적 지혜와 도덕적 사고가 있다. 정사유에서 자비심이라는 덕이 있으며, 정어 정업 정명에서는 표현의 자유 불허, 생명권 존중, 재산권 존중, 성적 존중, 직업 경제생활의 실천적 덕목의 중요성의 가르침이 있다. 정정진, 정념, 정정에서 악을 항상 경계하고 억제하면서 선을 계발하고 유지하려는 강력한 의지와 선을 행하되 항상 잊지 않고 기억하면서 잘못된 길로 빠지지 않는 덕목, 초월의 경지를 추구하는 가르침에 근거하여 정보윤리에 적용한다면, 정보화의 역기능 해소가 가능할 것이다. 따라서 팔정도를 통한 정보윤리 교육은 정보화의 비윤리적 행위를 억제하지만, 장기적으로 자비심이라는 덕을 형성하고, 통합적 인격을 형성하게 된다. 따라서 팔정도는 탁월한 정보윤리 교육 체계가 될 수 있는 가능성을 지니고 있다. There are numerous conveniences, which were obtained by information. However, even the occurrence of adverse effect caused by this is bringing about many ethical problems. Noble eightfold path is the most comprehensive moral system of the Buddhist ethics. Thus, the application of this will be able to solve the ethical problem of information. Noble eightfold path functions ethically by being harmonized eight virtue systems such as right view, right intention, right speech, right action, right livelihood, right effort, right mindfulness and right meditation. Here is contained the contents and doctrines available for solving ethical problem about information. Right view has doctrines such as Pratityasamutpada(Dependent origination or dependent arising), repentance, rational wisdom, and a moral thought. There is virtue called mercy in right intention. Right speech, right action, right livelihood have teaching of importance in non-permission on freedom of expression, a respect for a right of life, a respect for a right of property, a sexual respect, and practical virtue of occupational and economic life. In right effort, right mindfulness and right meditation, the application to information ethics will lead to being available for solving adverse effect of information based on teaching of the virtue of not falling into a wrong while always not forgetting and remembering with performing strong intention and goodness with the aim of developing and maintaining goodness as well as always looking about and inhibiting badness, and based on teaching of a pursuit for the transcendental state.

가야시대 고분 및 부장품 내에 존재하는 미생물의 다양성 조사

하병석 ( Byeong Seok Ha ),고선철 ( Seon Cheol Ko ),조아름 ( A Reum Jo ),김승락 ( Seung Rack Kim ),김상우 ( Sang Woo Kim ),노현수 ( Hyeon Su Ro ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.2

Microbial diversity of soil samples from ancient stone-lined tombs was investigated. The tombs, discovered at Eoryung Ocheon-Ri site, Korea, were estimated to be belonged to middle class people from an ancient country, Gaya, which existed till AD 559 at the southern part of Korea. Nine fungal stains and 70 bacterial strains were isolated from the twelve soil samples, which were collected from the tomb Nos. 5 and 6. Ribosomal DNA sequence analysis discovered 5 fungal and 22 bacterial strains belonged to 10 genus groups from the tomb No. 5 while 1 fungal and 28 bacterial strains belonged to 6 genus from the tomb No. 6. The higher microbial diversity suggests that the tomb No. 5 was constructed warmer season than the tomb No. 6. Moreover, the discovery of Staphylococcus warneri, which is found as part of the skin flora on human and animals, and Bacillus aquimaris, which is a marine bacterium and can be discovered from tidal flat, from the surface of large dagger suggests that the ancient people may use meat and seafood at the burial ceremony.

천식 및 만성 비부비동염을 동반한 환자의 비용에서의 Cytokine 발현

동헌종,하병석,김효열,김명순,정승규 대한이비인후과학회 2004 대한이비인후과학회지 두경부외과학 Vol.47 No.3

Background and Objectives:nes in rhinosinusitis with asthma have not been well understood. We investigated the pattern of cytokine production in chronic sinusitis in asthmatic patients and compare the findings in asthmatic vs. non-asthmatic patients. Materials and Method:We ana-lyzed cytokine levels in nasal polyps from 20 patients with sinusitis & asthma and from 25 patients with sinusitis alone. Evaluated parameters are the levels of the following cytokines:IL-3, IL-4, TGF-β1, IFN-γdisease extent evaluated by degre of polyposis. Results:There was no statistically significant diference between two groups on cytokine levels and preoperative polyposis. Conclusion:This study shows that there was no significant change in cytokine levels of sinusitis acording to the presence of asthma. These findings would suggest the existence of other mechanisms shared by sinusitis and asthma.