토양시료로부터 $\alpha$-glucosidase 저해제 생성 방선균의 분리

하남주,최성숙,정남용,김경제 한국미생물학회 2002 미생물학회지 Vol.38 No.2

탄수화물분해 효소인 $\alpha$-glucosidase의 활성을 저해하는 물질을 생성하는 미생물을 검색하기 위하여 토양시료로 부터 수종의 미생물을 검색하였다. 토양 시료에서 강력한 $\alpha$-glucosidase 저해제를 생성하는 방선균PM718을 분리하였고 그 저해제의 활성을 기지물질인 acarbose와 비교 측정한 결과 우수한 활성을 나타냄을 확인하였다. 또한 그 활성은 pH 변화에도 안정된 활성을 유지하는 것을 확인하였다. To find $\alpha$-glucosidase inhibitors produced by Actinomycetes, bacteria belonging to Actinomycetes were isolated from soil sample using Bennett's medium. The inhibitory activity induced by these bacteria on $\alpha$-glucosidase, which is the key enzymes far carbohydrates digestion and the prevention of diabetic complications, was investigated. A strain of these bacteria, PM718 potently inhibited $\alpha$-glucosidase activity in vitro.

Antimicrobial Activity of Commonly Used Antibiotics and DNA Fingerprint Analysis of Pseudomonas aeruginosa Obtained from Clinical Isolates and Unchlorinated Drinking Water in Korea, 2010

하남주,Jung Rae Kim,이도경,Hyang Mi An,Mi Jin Kim,Si Won Lee,Min Kyeong Cha,Kang Oh Lee 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.8

Pseudomonas aeruginosa exists in various environments, and can cause mild or serious infections resulting in a wide range of symptoms. In this study, we collected bacterial isolates from hospitalized patients and unchlorinated drinking water, in Korea, 2010. The water-borne and clinical isolates were compared using colony morphology, antimicrobial susceptibility testing,and random amplification of polymorphic DNA analysis. We first compared morphological features of the water-borne and clinical isolates. The clearest difference in colony morphology was colony shape; five water-borne isolate colonies (83%) had a smooth, circular morphology, while nine (75%) clinical isolate colonies had a rough, irregular morphology. Minimum inhibitory concentrations analyses were performed to determine antimicrobial resistant patterns; using ceftazidime, gentamicin, tigecycline, chloramphenicol, meropenem, and tobramycin according to Clinical and Laboratory Standard Institute (CLSI, 2009) methodology. All waterborne isolates were not resistant to gentamicin, tobramycin, and meropenem. The clinical isolates were resistant to every antibiotic except chloramphenicol. Genotyping was performed using the repetitive extragenic palindromic polymerase-chain-reaction. The DNA fingerprinting patterns did not reveal genetic similarity between the water-borne and clinical P. aeruginosa isolates. On the contrary, they showed that genetically distinct populations have been established in each of these environments. We have revealed significant morphological, clinical and genetic differences between water-borne and clinical isolates of the same bacterial species.

한국산 Rhizobium 속의 분리 및 동정

하남주,안태근,이영록,Ha, Nam-Ju,An, Tae-Kun,Lee, Yeong-Nok 한국미생물학회 1984 미생물학회지 Vol.22 No.2

From the root nodules of legumes distributed in South Korea, 74 strains of Rhizobium were isolated. The strains isolated were identified based on Bergey's Manual and Vincent's identification key. Following 5 species of Rhizobium were confirmed. R. leguminosarum, R. meliloti, R. phaseoli, R. trifolii, and R. japonicum

근신경등척성수기요법(MNIT)이 중년여성의 신체조성 및 혈관탄성에 미치는 영향

하남주(Nam Ju Ha),심은경(Eun Kyung Sim) 한국인체미용예술학회 2016 한국인체미용예술학회지 Vol.17 No.3

The purpose of this research is to analyze the effects of MNIT manipulative therapy on middle-aged women’s body composition and blood vessel elasticity. In order to do so, it selected a total of 22 married middle-aged women living in Ulsan-city with 30% of body fat ratio and 0.85-0.95%of abdominal fat ratio. Then, the research assigned 11 women in group MNIT where MNIT manipulative therapy was conducted and in 11 other in group CON where MNIT manipulative therapy was not carried out, and conducted MNIT manipulative therapy(abdomen) 3 times a week for 12 weeks from February 2, 2015 to April 24, 2015. Before and after the experiment on both groups, their physiological changes were examined in terms of their body composition (weight, skeletal muscle mass, body fat amount, body fat ratio, BMI, abdominal fat ratio, basal metabolic rate, lean mass amount, waist circumference, and hip circumference)and vessel elasticity (left and right upper and pelvic limbs) through a physiological test. In addition, the general characteristics, eating habits, exercise habits, management effect, and satisfaction of the women in group MNIT were studied before and after the experiment. The statistical data of the experiment was analyzed using SPSS(Statistical package for Social Science) v. 21.0, which generated the following results.

원유시료에서 분리된 반코마이신 저항성 Coagolase-Negative Staphylococcus 균주의 특성

하남주(Nam Joo Ha),최성숙(Sung Sook Choi) 대한약학회 2001 약학회지 Vol.45 No.5

Coagulase-negative Staphylococcus sp #39, isolated from raw-milk showed reduced sus- ceptibility to vancomycin. The minimun inhibitory concentration (MIC) for strain #39 was at 8μg of vancomycin per ml. Transmitting electron microscopy displayed that this strain had a 2.5∼3.5 times thicker cell wall than a vancomucin sensitive strain of Staphylococcus sp. The strain #39 also had an increased cell volume. These data indicate that the reduced susceptibility may be due to the thickness of the cell wall of the test strain.

운동선수의 베타3-아드레날린성 수용체 유전자의 Trp64Arg 다형성이 혈압, 신체조성 및 골밀도에 미치는 영향

정인근,오상덕,김태욱,강병용,하남주,하남주,Jung In Geun,Oh Sang Duk,Kim Tae Wook,Kang Byung Yong,Ha Nam Joo,Ha Nam Joo 대한약학회 2005 약학회지 Vol.49 No.1

The purpose of this study was to investigate the relationship between Trp64Arg polymorphism in the ${\beta}_3$-adrenergic receptor gene and complex phenotypes such as blood pressure, body compositions and bone parameters in young men about 20 years, and to collect the fundamental data in designing the exercise program. Eighty healthy young men including 41 controls and 39 athletes were recruited, Trp64Arg polymorphism in the ${\beta}_3$-adrenergic receptor gene was genotyped by PCR-RFLP method. By association study, there were no significance in genotype and allele frequencies of Trp64Arg polymorphism in the ${\beta}_3$-adrenergic receptor gene between controls and athletes, respectively (p>0.05). When the relationship between physiological parameters and Trp64Arg polymorphism in the ${\beta}_3$-adrenergic receptor gene was tested, this polymorphism was significantly associated with 3th lumber and left femoral neck Z-score values in controls (p<0.05), but these associations were not detected in athletic groups (p>0.05). It is likely that Trp64Arg polymorphism in the ${\beta}_3$-adrenergic receptor gene is a genetic marker for the bone mineral density index in young men, but environmental factors such as exercise modify the significant effect of this polymorphism. Thus, our results suggest that Trp64Arg polymorphism in the ${\beta}_3$-adrenergic receptor gene may be applicable as a predictive marker for osteoporosis in Korean young men, and regular exercise may prevent the disadventageous effect of this polymorphism for bone mineral density in male athletic group.

식이에 따른 장내세균의 효소활성 및 장내세균층의 비교

최성숙,하남주 한국미생물학회 1999 미생물학회지 Vol.35 No.2

The effects of diet on the composition of fecal microflora in mouse and the aclivilies of several enzymes in the leces were investigated. Vegetarian dietary groups were found to contain about ten lines higher numbers of Locmbocillus and B$&bacterizml than animal dietary groups. An~rnal dietmy groups were found to contain about 5 tolo times higher numbers of anaerobic Closhidia and Bocieriocles than the vegeterian detary groups. Fccal microbial $\beta$-glucosidase, $\beta$-glucm'onidase, ii-yptophanase and orease activilies in ihe animal dietary groups were shown lo be 30 to 50% hgher than those in h e vegetarian detary groups. 식이에 따른 장내세균의 균총의 변화와 장내미생물의 효소활성을 비교하였다. 동물성 식이를 실시한 군에서는 식물성 식이를 실시한 군과 비교시 유해한 혐기성 균의 수가 약 10배 정도 많게 관찰되었으며, 식물성 식이를 실시한 군에서는 비피더스균이나 유산간균의 수가 동물성 식이를 실시함 군보다 10배 정도 많게 관찰되었다. 또한 대장암 발병과 상관관계가 깊은 것으로 알려진 장내미생물 유래효소활성을 비교한 결과 $\beta$-glucosidase, $\beta$-glucuronidae, tryptophanase, urease 등의 효소활성이 동물성 식이를 실시한 군에서 1.8배-2배 가량 높게 나타났다. 즉 식이에 따라 장내 세균총의 차이 및 장내세균의효소활성의 현저한 차이가 있음을 알 수 있었다.

Association Study Between Vitamin D Receptor Gene Polymorphism and Adult Periodontitis in Korean

강병용,하남주 한국통합생물학회 2003 Animal cells and systems Vol.7 No.2

Adult periodontitis is a chronic inflammatory disease whose etiology is not well defined. Recent studies have shown that vitamin D receptor gene has been a candidate for the susceptibility of adult periodontitis. The purpose of this study is to investigate the frequency of Taq I restriction fragment length polymorphism (RFLP) in the vitamin D receptor gene in 141 periodontically healthy controls and 28 adult periodontitis patients. Taq I RFLP in the vitamin D receptor gene was detected by PCR amplification, followed by restriction enzyme digestion and 2% agarose gel electrophoresis. There was no significant difference in the distribution of Taq I RFLP between healthy controls and adult periodontitis group (P > 0.05). Thus, Taq I RFLP in the vitamin D receptor gene may not confer the susceptibility to adult periodontitis in Korean population. However, t allele distributions of this RFLP showed various frequencies among ethnic groups studied. Further studies in other ethnic groups will be required.

The Combination of Mixed Lactic Acid Bacteria and Dietary Fiber Lowers Serum Cholesterol Levels and Fecal Harmful Enzyme Activities in Rats

이도경,하남주,Shin Young Park,Seok Jang,Eun Hye Baek,Mi Jin Kim,Sun Min Huh,최경순,정명준,Jin Eung Kim,이강오 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.1

Probiotics such as lactic acid bacteria (LAB) and prebiotics such as fiber are generally considered beneficial for health. These affect the microflora composition and fermentation metabolites and consequently contribute to local and systemic effects in humans. The beneficial effects of probiotics can be improved when combined with prebiotics. Here we investigated the effects of a mixed LAB supplement combined with dietary fiber on the population of LAB in the gut, as well as on serum cholesterol levels, fecal water content and microbial harmful enzyme activities. For animal studies, 0.2 mL of mixed LAB (Bifidobacterium longum SPM1205, Lactobacillus acidophilus, and SAFELAC isolated from Pediococcus pentosaceus)supplement (10^7 ~ 10^8 colony forming units per day) was orally administered to male Sprague-Dawley rats every day for 2 weeks along with a diet containing 5% or 10% cellulose. The mixed LAB supplement combined with dietary cellulose significantly (p < 0.05) reduced serum total cholesterol and LDL levels. This combination also significantly (p < 0.05) increased the population of LAB and the fecal water content and significantly (p < 0.05) reduced microbial harmful enzyme (β-glucosidase, β-glucuronidase and tryptophanase) activities. These effects of LAB were particularly improved by its combination with 5% cellulose compared to the control (a diet without cellulose), and the 5% cellulose combination was more effective than the 10%cellulose combination. In conclusion, the incorporation of a fibrous diet such as cellulose with lactic acid bacteria improved the population of LAB, and daily consumption of this combination could reduce the serum cholesterol levels and activities of harmful enzymes such as β-glucosidase,β-glucuronidase, tryptophanase, urease in rats.

Antiviral effects of Lactobacillus ruminis SPM0211 and Bifidobacterium longum SPM1205 and SPM1206 on rotavirus-infected Caco-2 cells and a neonatal mouse model

강주연,이도경,하남주,신혜순 한국미생물학회 2015 The journal of microbiology Vol.53 No.11

Rotavirus is worldwide cause of severe gastroenteritis including severe diarrhea and fatal dehydration in infants and young children. There is an available vaccination program for preventing rotavirus infection, but it has limits and restrictions. Probiotics therapy could be an alternative method of antiviral prevention and modulation against rotavirus infection. In this study, we screened the antiviral activity of probiotic bacteria such as 3 Lactobacillus spp. and 14 Bifidobacterium spp. isolated from young Korean. Three of the bacteria, Lactobacillus ruminis SPM0211, Bifidobacterium longum SPM1205, and SPM1206, inhibited human strain Wa rotavirus infection in Caco-2 cells. Furthermore, these bacterial strains inhibited rotavirus replication in a rotavirus-infected neonatal mouse model. To clarify the mechanism of inhibition, we investigated gene expression of Interferon (IFN)-signaling components and IFN-inducible antiviral effectors. All 3 probiotics increased IFN-α and IFN- β levels compared with the control. Gene expression of IFNsignaling components and IFN-inducible antiviral effectors also increased. Overall, these results indicate that L. ruminis SPM0211, B. longum SPM1205 and 1206 efficiently inhibit rotavirus replication in vitro and in vivo. Especially, the antiviral effect of Lactobacillus ruminis SPM0211 is worthy of notice. This is the first report of L. ruminis with antiviral activity. Anti-rotaviral effects of the 3 probiotics are likely due to their modulation of the immune response through promoting type I IFNs, which are key regulators in IFN signaling pathway.