여대생의 월경곤란증 정도에 따른 골반정렬과 고관절 가동범위의 비교연구

최임순,장현정 대한정형도수물리치료학회 2015 대한정형도수물리치료학회지 Vol.21 No.2

서울시내 학교급식 실태조사

최임순 同德女子大學校 1984 同大論叢 Vol.14 No.1

A survey was made to investigate the overall status of school lunch program in Seoul area. Forty school lunch managing dieticians, each per one elementay school, among the total 54 schools in Seoul participated in the survey. Most of school lunch participants were the upper grade pupils in fourth to sixth grades. The dieticians were planning the school lunch menu at the intervals of one month using the basic five food groups on the basis of prof-icing approximately one-third of the Recommended Daily Dietary Allowances for ten-to twelve-year-old school children. Bread was more frequently served as main food item than cooked rice because of it's convenient preparation. All the school lunch programs included nutrition education which was conducted by distributing educational phamphlets or/and by lectures of classroom teachers or dieticians. The school lunch managing dieticians were proud of their job even though half of them weren't satisfied with their salary. A data obtained from the pupils of Hak-dong Elementary School showed that the school lunch participants were taller than the non-participant children. Most of the parents were in favor of school lunch program and believed that it has a positive influence on their children. The laborsome preparation and troublesome carrying of lunch-box were indicated by the parents and students, respectively, as one of the principal reasons of their participation in the school lunch program

Mouse의 Gonad가 人蔘抽出液에 의하여 받은 組織學的인 影響

崔林淳 연세대학교 대학원 1969 延世論叢 Vol.6 No.1

The effect of ginseng has been tested on gonad tissue of mouse, and the data revealed following results. 1. No appreciable changes have been detected in the testis and ovary both. 2. Development of testis appeared to be enhanced at the long term treatment. 3. In the ovary, which was treated for 30-day period, the atretic follicle was clearly observed.

배추흰나비(Pieris rapae L.)의 변태에 따른 중장상피세포와 Peritrophic membrane 의 미세구조 및 세포화학적 연구

최임순,최춘근,전진석 연세대학교 자연과학연구소 1981 學術論文集 Vol.7 No.-

In all insects, in fact in all arthropods, there are gross differentiations in foregut, midgut and hindgut on the embryological orgin of three regions (House, 1974). This study was accomplished to investigate the ultrastructures of midgut cells and peritrophic membrane in larval midgut of cabbage worm (Pieris rapae L.) by means of light microscope, transmission and scanning electron microscope. Experimental results were as follows; 1. The midgut epithelium is composed of three different types of cell, namely columnar cell, goblet cell and regenerative cell. 2. The surface area of outer plasma membrane of columnar cells is increased by regular projections called microvilli (Mv). In larvae of this insect, columnar cells may vary considerably in density of the cytoplasm and there is a great development of smooth endoplasmic reticulum (SER). Mitochondria are numerous in midgut cells but seem to show no special features in either structure or distribution. 3. The plasma membrane at the inner side of the columnar cells resembes that at the outer side in having the structure of a unit membrane but there may be a different appearance to the membrane at the base of the cells in contrast to that lining the infoldings. 4. The columnar cells are seperated from the basement membrane in later larval stage, therefore the debris (degenerated organelles or cytolysomes) of columnar cells protrude into the lumen of midgut. 5. The goblet cells in larvae appear to be of different nature from unicellular mucous gland of the vertebrate gut. This cell is very developed and there are many microvilli in lumen of goblet cell, and not opened into lumen of midgut. Other cell organelles are very similar to columnar cell. 6. The regenerative cells are cuboidal shape and a group of three or four cells localized inner side of basement membrane. The nucleus of regenerative cells are very larger than cytoplasm. 7. The peritrophic membrane, in thin sections viewed in an electron microscope may appears as a granular simple sheet or non fibrous layers. 8. The activities of acid phosphatase appeared excessively and alkaline phosphatase moderately.

쥐 간세포의 노화에 따른 형태학 및 생화학적 연구

최임순,주충노,최춘근,최치용,고기석,고지훈,곽한식 연세대학교 자연과학연구소 1983 學術論文集 Vol.11 No.-

생후 3개월부터 25개월까지 성장한 쥐를 나이군에 따라서 간세포의 미세구조적 변화를 전자현미경으로 관찰하고, DNA,RNA 및 단백질의 함량변화와 몇가지 효소활성의 변화를 관찰하였다. 미세구조적 변화로서 나이가 증가함에 따라 mitochondria의 크기가 커지며 수가 감소하는 현상이 나타나고 Lipid droplet가 늙은군에서 많아졌다. 한편 DNA,RNA및 단백질 함량의 변화 관찰로부터 나이의 증가에 따라서 단백질합성의 효율이 저하됨을 알 수 있으며 SDH, LDH, G6-PDH 효소 활성의 측정 결과 어린군에서는 성장에 필요한 생합성을 위하여 효소활성이 높아진다는 것을 알 수 있었다. 그러나 이러한 효소활동은 나이의 증가에 따라 점차로 감소되는 경향을 보였고, 아주 늙은 쥐에서의 효소활성은 크게 저하됨을 알 수 있었다. 이와같은 실험결과로 부터 세포는 노화됨에 따라 대사 기능이 저하되고 아주 늙은 쥐에서는 mitochondria와 핵과 같은 세포소기관의 형태적 변화까지 초래되는 것으로 생각된다. The liver cells of normal rats of young (3 month and 6 month old), middle age (9 month, 12 month, and 15 month), old age (18 month, 20 month, and 23 month old) and very old age (25 month old) were investigated morphologically as well as biochemically. Observation of ultrastructure of the cells of normal rats using electron microscope showed that the number of the mitochondria was decreased as aging but their size became larger at their older age. Furthermore, the lipid droplet in the cytoplasm appeared frequently in the cells of older group. The DNA, RNA, and protein analysis showed that the efficiency of the protein biosynthesis became lowered as aging. The activities of succinate dehydrogenase, glucose 6-phosphate dehydrogenase and lactate dehydrogenase were also gradually lowerd as aging. From the above experimental results, it seemed likely that the cell activities became gradually lower and the physiological activities of the extremely old rats became extremely low and some morphological changes of organells such as mitochondria and nucleus occured.

초기 계배의 뇌형성에 미치는 몇가지 요인에 관한 세포 생물학적 연구 (1) Serotonin의 영향

최임순,주상옥,주충노,오억수,신길상 한국통합생물학회 1989 동물학회지 Vol.32 No.1

Tryptophan 또는 serotonin을 계란에 투여하고 배양하였을 때의 초기 계배의 뇌형성에 미치는 영향을 관찰한 결과, 뇌의 신경세포는 핵막의 불규칙하고 핵응축현상이 심하며 염색질이 핵막에 응집되고 인도 분해되었으며 핵막이 팽출되어 생긴 수포가 형성되었다. 그리고 조면소포체와 골지체는 심하게 확장되어 있었고, 미토콘드리아는 팽윤화된 것이 많았으며 신경세관의 발달이 매우 미비하였다. 즉 이와 같은 현상은 특히 초기 1-2일간 배양 계배에서 극심하였다. Tryptophan 또는 serotonin을 투여한 후 18시간 배양한 배반엽의 간충 세포는 대조군에 비하여 세포질이 미숙하였고, 세포내 소기관이 크게 손상되어 있었으며 난황과립의 분해가 대조군에 비하여 지연되고 있음을 알 수 있었으며, 24-72시간 배양한 배반엽의 암대의 미세융모는 대조군에 비하여 현저하게 길이가 짧고 발달이 미비하였다. 또한 Tryptophan 또는 serotonin을 투여한 계배의 경우 배반엽의 크기, 투명대 및 배의 크기가 대조군에 비하여 작았으며, serotonin 처리군의 경우가 더욱 작았다. 또한 단백질 및 핵산 함량이 대조군에 비하여 훨씬 낮았고 tubulin 합성량도 크게 저하되었음이 확인되었다. 위와 같은 실험결과는 과량의 tryptophan 또는 serotonin이 난황과립 분해를 지연시켜 결과적으로 단백질 합성을 억제함으로서 tubulin합성이 저하되고, 이로 인하여 미세소관의 형성과 미세융모의 발달과 기능이 부진하여 형태형성에 이상을 초래하는 것으로 사료된다. The effect of tryptophan or serotonin on the early stage of chick brain development has been morphologically investigated using an electron microscope. The electron micrographs of neural plate cells of 1-day chick embryo treated with tryptophan or serotonin showed irregularity, evagination and disruption of nuclear membrane and nuclear chromatin condenstation, nucleolar margination and segregation. Hypertrophy of stalks, vesicles and vaculoes were seen and dilated and disrupted rough endoplasmic reticulum and underdeveloped neurotubules were also observed. In mesenchyme cells of tryptophan or serotonin administered 18 hr embryo, irregular nuclear membrane, swollen mitochondria, dilated rough endoplasmic reticulum and very large yolk granules were observed. Furthermore, DNA, RNA and protein contents of the embryos treated with typtophan or serotonin were considerably lower than those of control group. The amount of tubulin of the experimental groups was also greatly lower than that of control, suggesting that the impairment of microtubule formation occurred. Tryptophan or serotonin administration might depress the biosynthesis, of nucleic acid and protein including some enzymes tested. It seems that the serotonin formed from exogeneous tryptophan might inhibit the degradation of yolk granule by feedback regulation mechanism so as to impair microtububle and microvilli formation followed by a malformation of chick embryos.

자연 환경속의 몇몇 중금속이 갑각류의 발생에 미치는 영향과 세포내 침착에 관한 연구

崔林淳,崔春根,劉寬凞 연세대학교 자연과학연구소 1978 學術論文集 Vol.2 No.-

중금속들이 Artemia salina의 nauplius유생에 미치는 영향을 규명하기 위하여 수은, 카드미움, 구리를 각각의 농도 별로 인공해수에 희석 후 이들을 노출시켜 전자 현미경적 방법을 사용하여 각각의 미세구조의 변화에 대한 연구를 수행한 바 다음과 같은 결론을 얻었다. 가장 뚜렷한 변화는 모든 실험군에서 mitochondria가 팽창되었고 고 농도에서 이들의 막이 상당히 굴곡되었으며 cristae가 파괴되어 내부 기질의 전자 밀도가 낮은 것이 관찰되었다 수은 처리군에서는 핵의 chromatin의 농축화, lysosome의 출현, 활면소포체의 증가가 관찰되었으며 카드미움 처리군은 chromatin의 변화와 대형 지방적이 관찰되었다. 또한 구리 처리군은 활면 소포체의 부분적인 증가, 지방적의 출현과 chromatin이 분산되는 경향을 나타내었다. Electron microscopic studies were made to investigate effects of mercury, cadmium and copper on nauplius of Artemia salina by observing changes in the fine structure following their exposure to several concentrations of the heavy metals. Experimental results obtained are as follows: 1. In all treated groups, swollen mitochondria were observed at lower concentrations but irregular mitochondrial membrane as well as structural changes of cristae and lower electron density of mitochondrial matrix were observed in higher concentrations of heavy metals. 2. In mercury treated group, it was observed that clumping of nuclear chromatin, appearance of lysosomes and increases of smooth endoplasmic reticulum were characteristic. 3. Cadmium treatment resulted in changes of chromatin and increase in the number of large lipid droplets. 4. In copper treated group, chromatin tended to disperse throughout karyoplasm in the nucleus and partial increases of smooth endoplasmic reticulum, appearance of lipid droplets were apparant in the cytoplasm. 5. It may be concluded from these results that the fine structural changes induced by heavy metals treatment activate the detoxication processes and impair the energy metabolism.

흰쥐 간조직의 세포내 비소결합물질의 특성에 관한 연구

최임순,부문종,김충현 한국통합생물학회 1990 동물학회지 Vol.33 No.4

흰쥐 간세포에 존재하는 비소결합물질의 특성을 조사하기 위하여 4 ppm의 비소를 포함하는 NaASO$_2$수용액의 식수로 15일간 흰쥐에 공급하였다. 비소처리군의 간 cytosol분획의 정상단백 질의 함량은 감소하였으나 8종류의 stress protein의 함량은 증가하였다. Cytosol에 존재하는 비소결합체물질은 한 종류이고,glycine, glutamic acid 및 cysteine의 3종류의 아미노산으로 구성되어있으며 분자량은 500D이었다.Glutathione은 비소와 5:1의 몰비로 결합하였으며 glutathione과 비소의 복합체는 gel filtration chromatography에서 비소결합물질과 같은 이동성을 나타내어 cytosol에 존재하는 미소결합물질은 glutathione으로 추정되었다. Glutathione에 결합한 비소는 미토콘드리아의 호흡, 형태전환 및 팽윤과 수축기능에 있어서 대조군과 별다른 차이를 나타내지 않았다. 이와 같은 결과들로써, 생체에 직접 처리한 비소에 의하여 stress protein합성이 촉진되며, 간 cytosol에 존재하는 비소결합물질은 glutathione으로 추정되고, 비소와 복합체를 형성함으로써 비소의 세포독성에 대한 방어기능을 나타내는 것으로 생각된다. Sodium arsenite (As) aqueous solution containing 4 ppm wss given to male rats for 15 days as drinking water. Electrophoretic pattern of liver cytosol from As-fed rats appeared to be significantly different from that of the control rats. Although the normal protein content of the cytosol fraction of As-fed rat liver was decreased, 8 stress proteins were increased. In liver cytosol fractions of As-treated rat, one kind of arsenic-binding substance (AsBS) was observed. Molecular weight of AsBS was identified to be 500 D and composition of amino acid was glycine, glutamic acid and cysteine. Glutathione (GSH) appeared to bind to arsenic and GSH-As complex showed the same mobility as AsBS on gel filtration chromatography. GSH conjugated As prevented As from inhibiting respiration, conformational change and swelling-contration of mitochondria. According to the above results. it is concluded that in vfuo treated arsenic stimulated synthesis of stress protein, and arsenic-binding substance might be glutathione and have a protective role against arsenic toxicity.

열충격 및 ${\alpha}$-amanitin과 cycloheximide의 처리를 통한 초기 계배의 체절 형성 기작에 대한 발생학적 연구

최임순,박용빈,김옥용,Choe, Rim-Soon,Park, Yong-Bin,Kim, Ok-Yong 한국현미경학회 1991 Applied microscopy Vol.21 No.2

In order to investigate the factors of the control mechanism of somitogenesis, early chick embryos (H-H stage $8{\sim}13$) were treated with heat shock, ${\alpha}$-amanitin and cycloheximide and morphological changes of somite were examined by light microscopy, transmission and scanning electron microscopy. In normal chick embryo, somites were formed from the somitomere which preexisted in segmental plate. Somites were wrapped with extracellular collagen fibrils and connected with neural tube, notochord and ectoderm. And then, somites were differentiated to sclerotome, dermatome and myotome by the interaction of nervous tissue. Abnormal somites were observed after formation of six or seven so mites in heat shock treated group. Amounts of collagen fibrils were obviously decreased in this group. In cycloheximide treated group, most so mites were smaller and neural tube formation was incomplete. Chromatins were condenced and formed several heterochromatins in the nucleus of somite cells. Lipid like cytoplasmic dense mass and lipid droplets were also observed. Segmentation of somites seemed to be normal progress in ${\alpha}$-amanitin treated group. Center of somite, however, hollowed in longitudinal sectioned samples. These results suggested that so mites were already existed in the segmental plate as the form of somitomere. Segmented somites were contacted with neural tube or notochord and the somites were tightly connected with each other by the extracellular collagen fibrils which were secreted from neuroepithelium and somite cells. Somites are thought to differentiate into sclerotome, dermatome and myotome by these interactions.

초기 계배에 대한 Actinomycin D와 Puromycin의 영향

최임순,주충노,최춘근,유동석,Choe, R.S.,Joo, C.N.,Choi, C.K.,Reu, D.S. 한국현미경학회 1984 Applied microscopy Vol.14 No.2

Chick embryos received a single injection of actinomycin D($0.1{\mu}g,\;0.05{\mu}g\;or\;0.1{\mu}g$) or puromycin($10.0{\mu}g,\;30.0{\mu}g\;or\;50.0{\mu}g$) into the yolk sac of Arbor acres chick embryos either prior to incubation or at certain periods of time (48, 96 and 144 hours) after incubation. After 10days of incubation, surviving embryos were investigated morphologically and biochemically. Embryos treated with actinomycin D or puromycin showed a high mortality when they were exposed prior to incubation and at 48 hours after incubation. Electron micrographs of chondrocytes in tarso-metatarsal of antibiotics (actinomycin D or puromycin) treated embryos showed the destruction of cytoplasm and nuclei when they were exposed prior to incubation. Endoplasmic reticulum was expanded and mitochondria were damaged in chondrocytes of surving embryos treated with low doses at 48 hours, 96 hours or 144 hours after incubation. The activities of enzymes such as lactate dehydrogenase, malate dehydrogenase and succinate dehydrogenase in embryos treated with actinomycin D or puromycin were much less than those of the saline treated group. Also, the amounts of DNA, RNA and protein were greatly decreased.