GABA-enriched Fermented Laminaria japonica Protects against Alcoholic Hepatotoxicity in Sprague-Dawley Rats

차재영,이배진,제재영,강영미,김영목,조영수 한국수산과학회 2011 Fisheries and Aquatic Sciences Vol.14 No.2

The sea tangle, Laminaria japonica has long been used in Korea as a folk remedy to promote health. Gamma-amino butyric acid-enriched (5.56% of dry weight) sea tangle was obtained by fermentation with Lactobacillus brevis BJ-20 (FLJ). A suppressive effect of FLJ on carbon tetrachloride-induced hepatotoxicity has been shown previously. Alcohol administration to Sprague-Dawley rats leads to hepatotoxicity, as demonstrated by heightened levels of hepatic marker enzymes as well as increases in both the number and volume of lipid droplets as fatty liver progresses. However, FLJ attenuated alcohol-induced hepatotoxicity and the accumulation of lipid droplets following ethanol administration. Additionally, FLJ increased the activities and transcript levels of major alcohol-metabolizing enzymes, such as alcohol dehydrogenase and aldehyde dehydrogenase, and reduced blood concentrations of alcohol and acetaldehyde. These data suggest that FLJ protects against alcohol-induced hepatotoxicity and that FLJ could be used as an ingredient in functional foods to ameliorate the effects of excessive alcohol consumption.

부추(Allium tuberusum) 씨의 이화학적 특성과 항산화 활성

차재영,김성규,김현정,송재영,조영수 한국생명과학회 2000 생명과학회지 Vol.10 No.3

식용식물 이면서 한방재료로 이용되고 있는 부추의 이용 효율을 증대시킬 목적으로 부추씨의 이화 학적 특성과 수용성 추출물의 항산화 활성을 측정하였다. 부추씨의 일반적 조성을 보면 조단백질 25.7%, 조지방 16.6%, 조회분 2.9%, 수분 4.4%로 나타났다. 아미노산 조성은 건물 중량 100g 당 proline 11.0 g 으로 가장 높았으며, glutamic acid 4.91 g, arginine 2.12 g, aspartic acid 1.62 g, leucine 1.31 g, valine 1.16 g, methionine 1.15 g 순으로 나타났다. 부추씨의 무기질 성분은 칼륨 215 ppm, 칼슘 142 ppm, 철 124 ppm, 마그네슘 100 ppm 순으로 나타나 칼슘과 철분을 많이 함유하였다. 주요 지방산으로 linoleic acid (C18:2) 71.9%, oleic acid(C18:1) 12.7%, palmitic acid(C16:0) 8.6% 및 stearic acid(C18:0) 1.4% 순 으로 전체의 약 93%를 차지하였다. 부추씨로부터 열침 온도에 따른 총폴리페놀 화합물의 함량 변동은 2$0^{\circ}C$ 추출물에 비해 4$0^{\circ}C$ 추출물에서 27%, 6$0^{\circ}C$ 추출물에서 51% 증가하였다. 부추씨 수용성 추출물을 DPPH 환원법으로 항산화 활성을 측정한 결과, 부추씨는 BHT 보다 활성이 나타났다. 이상과 같이 부추 씨는 아미노산, 무기질 및 지방산을 풍부하게 함유하고 있어 영양적인 면에서도 상당한 가치를 가지는 식품재료로 이용될 가능이 있는 것으로 사료된다. The chemical compositions as amino acids, minerals, fatty acids, and total polyphenolic compounds of the seeds of leek (Allium tuberusum) were analyzed. The antioxidative activity of water soluble extract from leek seeds was also tested in DPPH ($\alpha$, $\alpha$ - diphenyl-$\beta$ -picrylhydrazyl) method. The chemical compositions of leek seeds were moisture 4.4%, curde protein 25.7%, crude fat 16.6%, and crude ash 2.9%. Major amino acid compositions were proline 11 g, glutamic acid 4.9 g, arginine 2.1g, aspartic acid 1.6g, leucine 1.3g, valine 1.2 g, and methionine 1.1 g as per 100g. Mineral contents were K 215 ppm, Ca 142 ppm, Fe 124 ppm, and Mg 100 ppm. Major fatty acid compositions were linoleic acid 71.9%, oleic acid 12.7%, palmitic acid 8.6%, and stearic acid 1.4%. The changes of contents in polyphenolic compound from leek seeds caused by heat treatment were also listed in the following order; $20^{\circ}C$(364mg/100g), $40^{\circ}C$(462 mg/100g), and $60^{\circ}C$(551 mg/100g). Antioxidative activity as electron donating ability showed in the following order; 0.05% BHT(butylated hydroxytoluene)(45.6%)>0.05% water-extract(31.3%)>0.1% water extract(30.3%). On the basis of chemical analysis, the leek seedsshowed to have relatively high contents of nutrients as amino acids, minerals, fatty acids.

옻나무 수피 추출물이 마우스의 지질농도 및 지질과산화에 미치는 영향

차재영,조영수 한국생명과학회 2000 생명과학회지 Vol.10 No.5

Male mice (ddY strain) were fed a laboratory chow diet containing the water extract from stem bark of Rhus verniciflua Stokes (RVS) for 14 days. There were no significant difference in body weight gain, feed intake, the hepatic lipid profile and serum total cholesterol and phodpholipid concentrations. The concentration of triglyceride in serum was significantly lower in the RVS group than that in the control group. The concentration of high-density-lipoprotein cholestrol in serum was significantly higher in the RVS group than that in the control group. The methanol extract from RVS stem bark effectively inhibited the formation of thiobarbituric acid-reactive substances as a marker of lipid peroxidation of liver microsomes in a concentration-dependent manner. This study showed that the water extract from stem bark of RVS decreased the serum triglyceride concentration and methanol extract has an antioxidative activity.

마우스의 간장과 혈청 지질농도에 미치는 타우린 및 오로트산의 영향

차재영,조영수 한국생명과학회 1999 생명과학회지 Vol.9 No.6

Male mice (ddY strain) were fed with the chow diet containing 10% sucrose supplemented with orotic acid at the 1% level or/and taurine at the 5% levels for 14 days. The concentrations of triacylglycerol and cholesterol in the liver were significantly lower in the orotic acid group than the control group. When taurine and orotic acid were administered simultaneously, the concentrations of triacylglycerol and cholesterol in the liver were higher and lower, respectively, compared to the orotic acid group. The concentration of triacylglycerol in the serum was higher in the taurine group than that of the control or the orotic acid groups, and the simultaneous supplementation of orotic acid and taurine further enhanced. There were no significantly difference in body weight gain, diary food intake, and the concentrations of serum cholesterol and hepatic phospholipid. These results suggest that dietary taurine stimulated the increasion of hepatic triacylglycerol by orotic acid in mouse.

오르트산 투여 마우스의 지질농도에 미치는 Hesperetin 및 Naringenin의 영향

차재영,김석영,정순재,조영수 한국생명과학회 1999 생명과학회지 Vol.9 No.4

Male mice (ddY strain) were fed a laboratory chow diet containing 10% sucrose supplemented with orotic acid, hesperetin or naringenin at the 1% level for 14 days. the concentrations of liver triacylglycerol and cholesterol were significantly lower in the OA group than in the control group. When both flavonoids and orotic acid were administered simultaneously, the orotic acid-dependent decrease in liver triacylglycerol and cholesterol were attenuated slightly. The concentration of serum cholesterol in the orotic acid group or the control group was lower than in the orotic acid groups supplemented with hesperetin or naringenin. There were no significantly difference in body weight gain, diary food intake, and the serum concentrations of triacylglycerol and high-density-lipoprotein cholestrol. It was concluded that the inducement of fatty liver in mice failed to feeding a laboratory chow diet containing 10% sucrose supplemented with 1% orotic acid for 14 days.

유류오염 지역에서 분리된 Klebsiella sp. KCL-2에 의한 원유분해 특성

차재영,김혜선,조영수,이영춘,최용락 한국생명과학회 2000 생명과학회지 Vol.10 No.3

원유 분해능이 강력한 해양균주를 얻고자 유류오염 지역으로부터 crude oil을 탄소원으로 이용하는 수집 종을 분리하였다. 분리된 균주중 원유분해능 및 성장속도면에서 가장 우수한 균주를 선별하여 KCL-2로 명명하였으며, 형태학적, 생화학적 및 생리학적 특성을 조사한 후 Klebsiella sp.로 동정하였다. KCL-2 균주의 원유 분해를 위한 최적 배양조건은 배양온도 27$^{\circ}C$~37$^{\circ}C$였으며 초기 pH는 7.0이였다. 또 한, 이 균주의 성장은 3.0% 염분농도에서 최대의 성장을 보여주어 해양유래의 균주임을 확인하였다. 비 교적 장쇄인 n-alkane 계 탄화수소의 C18~C28의 탄화수소를 탄소원으로 이용하였다. 원유분해시 C18 의 첨가에 의해 균생육 및 원유 분해능이 촉진되는 것으로 나타났다. KCL-2 균주의 유화활성은 32$^{\circ}C$, pH 7.0의 배양조건에서 배양 3일째에 가장 높게 나타났다. Several bacterial strains utilizing crude oil as their sole carbon and energy source were isolated from polluted marine by crude oil. One of the strains, named KCL-2 showed strong degradation activity for crude oil. This strain was identified as a Klebsiella sp. based on the morphological, biochemical, and physiological characteristics. The optimum cultural conditions were as follows; $27^{\circ}C$~$37^{\circ}C$ for temperature and 7.0 for initial pH. Additionally, the optimal concentration of sodium chloride was 3.0%, confirming indicating that this strain was derived from sea water.The strain KCL-2 could use several kinds of n-alkane hydrocarbones from octadecane to octacosane as a sole carbon source. The emulsifying activity by KCL-2 was the highest after 3 days of cultivation under the condition of 3.0% sodium chloride, pH 7.0 and 32$^{\circ}C$. This strain had several criptic plasmids.

익형과 엔진 종류를 고려한 도로주행형 PAV 초기 사이징

차재영,황호연 한국항행학회 2019 韓國航行學會論文誌 Vol.23 No.1

In many countries, there are needs of new transportations to replace ground congestions due to growing number of cars. In addition, the increase in the number of cars held by economic growth will further increase traffic congestion in the future. To overcome this problem, many researches have been performed for personal air vehicle (PAV). In this study, the wing loading and the power-to-weight ratio that are major design parameters for the sizing of roadable PAVs were calculated for different kinds of airfoil and engine types. I.e., in the sizing process, the study was conducted to determine the design point using the graphs of wing loading, power-to-weight ratio, brake horse power, and fuel efficiency for the given mission profiles considering domestic environments and the FAR PART 23 which is the GA class aircraft certification standard. As a result of sizing, using diesel engine require high maximum take-off weight, wing area, and power compared to gasoline engine due to more engine weight. 많은 나라들에서 늘어가는 자동차로 인한 교통체증에 대체 할 수 있는 새로운 교통수단에 대한 요구가 증가하고 있다. 또한, 경제적인 성장으로 인한 자동차 보유 대수의 증가로 앞으로 교통체증은 더욱 증가할 것이다. 이를 위해 도로주행형 개인용 항공기 (PAV; personal air vehicle)에 관한 많은 연구가 진행 되고 있다. 본 연구에서는 도로주행형 PAV의 사이징에서 엔진과 익형의 종류에 따른 주요 설계 변수인 익면하중과 동력대중량비를 구하기 위한 사이징을 수행하였다. 즉, 사이징 과정에서 국내 환경과 일반항공기급 인증기준인 FAR PART 23을 고려한 임무형상에 대하여 익면하중, 동력대중량비, 제동마력, 그리고 연료효율의 그래프를 통해서 설계점을 결정하는 연구도 수행하였다. 사이징 결과 디젤 엔진을 사용하는 경우 가솔린 엔진보다 더 큰 중량으로 인해 더 큰 이륙총중량, 날개 면적, 그리고 더 큰 동력을 요구한다.

실내 NLOS환경에서 정밀도 향상을 위한 IEEE 802.15.4a 기반의 위치추정 알고리즘

차재영,공영배,최증원,고종환,권영구,Cha, Jae-Young,Kong, Young-Bae,Choi, Jeung-Won,Ko, Jong-Hwan,Kwon, Young-Goo 한국정보통신학회 2012 한국정보통신학회논문지 Vol.16 No.8

IEEE 802.15.4a 표준은 정확한 위치추정 서비스를 지원하기 위하여 time-of-arrival (TOA) 기반의 거리측정 기술을 제공한다. 특히, non-line-of-sight (NLOS) 상황은 TOA 기반의 위치인식에서 중요한 문제이고, IEEE 802.15.4a 네트워크의 성능에 심각한 영향을 끼칠 수 있다. 본 논문에서는 에너지 효율적인 낮은 복잡도의 위치추정 알고리즘을 제안한다. 제안한 알고리즘은 TOA 거리측정을 위해 순차적인 acknowledgment (ACK) 패킷을 이용하고 위치추정지역을 제한 할 수 있는 작은 사각 영역을 설정함으로써 위치를 추정한다. 패킷 교환으로 인한 메시지 오버헤드는 순차적 ACK 패킷을 이용하여 감소될 수 있다. 사각 영역 설정을 통하여 NLOS 환경에 있는 거리측정 값을 제거하고, 위치 추정의 영역을 제한함으로써 추정하는 연산 시간과 에너지를 줄일 수 있다. 시뮬레이션을 통해 에너지 소모와 추정 위치의 정확도 측면에서 제안한 알고리즘이 기존의 알고리즘 보다 좋은 성능을 보이는 것을 확인 하였다. IEEE 802.15.4a standard can provide a variety of location-based services for ZigBee or wireless network applications by adapting the time-of-arrival (TOA) ranging technique. The non-line-of-sight (NLOS) condition is the critical problem in the IEEE 802.15.4a networks, and it can significantly degrade the performance of the TOA-based localization. To enhance the location accuracy due to the NLOS problem, this paper proposes an energy-efficient low complexity localization algorithm. The proposed approach performs the ranging with the multicast method, which can reduce the message overhead due to packet exchanges. By limiting the search region for the location of the node, the proposed approach can enhance the location accuracy. Experimental results show that the proposed algorithm outperforms previous algorithms in terms of the energy consumption and the localization accuracy.

함초 요구르트가 Streptozotocin- 유발 당뇨 쥐에 미치는 생리학적 효과

차재영,전병삼,김범규,주영광,박상우,조영수 한국생명과학회 2005 한국생명과학회 심포지움 Vol.45 No.-

감귤류 플라보노이드의 지질 과산화물 생성에 미치는 항산화 효과

차재영,김현정,김성규,김민석,정병희,조영수 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.27 No.-