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신용철,이종호,진오성,이은지,진인화,김창석,홍석원,한동욱,김준태,오진우 한국물리학회 2015 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.66 No.1
Extracellular matrices (ECMs) are network structures that play an essential role in regulatingcellular growth and differentiation. In this study, novel nanofibrous matrices were fabricated byelectrospinning M13 bacteriophage and poly(lactic-co-glycolic acid) (PLGA) and were shown to bestructurally and functionally similar to natural ECMs. A genetically-engineered M13 bacteriophagewas constructed to display Arg-Gly-Asp (RGD) peptides on its surface. The physicochemical propertiesof RGD peptide-displaying M13 bacteriophage (RGD-M13 phage)/PLGA nanofibers werecharacterized by using scanning electron microscopy and Fourier-transform infrared spectroscopy. We used immunofluorescence staining to confirm that M13 bacteriophages were homogenously distributedin RGD-M13 phage/PLGA matrices. Furthermore, RGD-M13 phage/PLGA nanofibrousmatrices, having excellent biocompatibility, can enhance the behaviors of vascular smooth musclecells. This result suggests that RGD-M13 phage/PLGA nanofibrous matrices have potentials toserve as tissue engineering scaffolds.
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Cytotoxicity Evaluations of Pristine Graphene and Carbon Nanotubes in Fibroblastic Cells
이종호,신용철,진오성,이은지,한동욱,강석희,홍석원,안지영,김수형 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.6
This study concentrates upon investigating differential cellular responses to carbon nanoparticles (CNPs) such as pristine graphene flakes, single-walled carbon nanotubes (SWCNTs) and multiwalled CNTs (MWCNTs), in human dermal fibroblasts (HDFs) <i>vs</i>. the L-929 fibroblast cell line. Characterizing the surface morphology of each CNP by using scanning electron microscopy, we determined their cytotoxicity profiles by quantifying the mitochondrial activity. Graphene was found to have an irregular flake-like shape with various lengths within the ranges of 50 ∼ 1500 nm. The estimated sizes of the SWCNTs and theMWCNTs were about 400 nm and 1000 nm in diameter, respectively and several μm in length. All the CNPs tested here exerted adverse effects on the viability of HDFs even at 15.6 ppm, through intracellular uptake whereas except MWCNTs, they did not show any cytotoxicity against L-929 cells at 250 ppm. This result suggests that CNPs can have a differential influence on normal fibroblasts <i>vs</i>. immortalized ones.
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Enhanced Neurite Outgrowth of PC-12 Cells on Graphene-monolayer-coated Substrates as Biomimetic Cues
이종호,신용철,진오성,한동욱,강석희,홍석원,김종만 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.10
Neurons are electrically excitable cells that transmit and process information in the nervous system. Recently, it has been shown that the differentiation of human neural stem cells to neurons is enhanced on graphene substrates, and differentiated neurons can still carry electrical signals when stimulated by the graphene electrodes. Graphene films grown by chemical vapor deposition were transferred onto a glass coverslip by scooping method and then coated with fetal bovine serum for neuronal cell culture. The graphene substrates as biomimetic cues have been shown to enhance the neurite outgrowth of PC-12 cells. Our findings suggest that graphene has a unique surface property that can promote the neuronal cells, which should open up tremendous opportunities in neuroscience, neural engineering and regenerative medicine.
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냉동보존 기간 동안 L-929 섬유아세포의 생존에 대한 세포내 트레할로즈의 보호 효과 및 작용 기작
이종호,김현용,신동명,황윤회,진오성,김형국,한동욱 한국생체재료학회 2010 생체재료학회지 Vol.14 No.3
Recently, there has been much interest in using trehalose and other small carbohydrates to preserve mammalian cells in the dried state as an alternative to cryopreservation. In this study, we have aimed at testifying the hypothesis that the deleterious effects of cryopreservation of mammalian cells can be ameliorated by the addition of disaccharides,such as sucrose, maltose and trehalose, to the storage media. For this purpose, the protective effects of disaccharides on unilamella vesicles (ULVs) of dimyristoylphosphatidylcholine (DMPC) and L-929 fibroblasts were investigated by monitoring the change of ULVs diameter and assaying the cell viability, respectively. The mean diameters of ULVs with different disaccharide contents were measured by using photon correlation spectroscopy. For investigating the cryoprotective effects of intracellular disaccharides, L-929 cells were cryopreserved in complete culture media containing various concentrations (0.5, 1.0 and 2.0 mM) of sucrose, maltose or trehalose at −25oC, −75oC and −150oC during 4 d. After quick thawing at 37oC, the cellular viability was determined with water-soluble tetrazolium salt assay. Cryoprotective effects of trehalose were better than those of other disaccharides and DMSO. The mechanism of cryoprotective action of intracellular trehalose could be explained in part by the high flexibility of glycosidic bond in trehalose molecules and their interaction with phospholipid bilayers of cells. It is suggested that trehalose may be effectively applied for various fields as the storage of cell therapeutics and biological agents, the longer-term preservation of transplantable tissues and artificial organs as well as tissue engineering and regenerative medicine
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이은지,신용철,Dong-Gook Hwang,김진수,진오성,Linhua Jin,홍석원,한동욱 한국생체재료학회 2014 생체재료학회지 Vol.18 No.1
In this study, novel graphene oxide (GO)-decorated hybrid fiber sheets composed of poly(lactic-co-glycolic acid, PLGA) and collagen (Col) (GO-PLGA/Col) for application to tissue engineering scaffolds were prepared via dual electrospinning. Physicochemical properties of GO-PLGA/Col fiber sheets were characterized by field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), Fourier transform infrared (FTIR) and Raman spectroscopy, thermogravimetric analysis (TGA) and contact angle measurement. FESEM and AFM images showed that GO-PLGA/Col fiber sheets had a three-dimensional interconnected pore structure with an average fiber diameter of about 480 nm. FTIR and Raman spectra revealed that GO was uniformly distributed in the fiber structure of PLGA or PLGA/Col sheets. TGA profiles demonstrated that GO-PLGA/Col hybrid fiber sheets were thermally stable in spite of adding GO. GO slightly affected the contact angle of PLGA sheets, while Col significantly increased their hydrophilicity. Initial attachment of human dermal fibroblasts (HDFs) on GO-PLGA and GO-PLGA/Col fiber sheets was significantly superior to that on PLGA sheets, and their proliferation was gradually increased during the culture period. These results suggest that GO-PLGA/Col hybrid fiber sheets can be effectively used as scaffolds supporting tissue regeneration.
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