여름철 젖소 사양관리

지병주 한국낙농육우협회 1991 낙농·육우 Vol.11 No.7

소에 의한 다양한 사일리지의 이용율 및 TDN 평가

지병주(Byung Ju Ji),김광림(Guang Lin Jin),Judder Shinekhuu,Wei-ze Qin,오영균(Young Kyoon Oh),손용석(Yong-Suk Sohn),서성(Sung Seo),송만강(Man Kang Song) 한국초지조사료학회 2010 한국초지조사료학회지 Vol.30 No.2

본 시험은 국내에서 생산되어 곤포형태로 포장된 청보리 사일리지, 호밀 사일리지, 이탈리안 라이그라스 사일리지 및 수단그라스 (수수 × 수단그라스 교잡종) 사일리지의 반추위 내 발효특성과 분해율 및 전장소화율과 TDN을 평가하기 위해 반추위 누관이 장착된 소 4두를 대상으로 실시되었다. 사일리지가 반추위액의 pH에 거의 영향하지는 않았으나 사일리지 급여 후 3시간에서 다른 종류의 사일리지를 섭취한 경우와 비교할 때 수단그라스 사일리지를 섭취한 소의 반추위액의 암모니아 농도가 7.28㎎/100 ml로 가장 낮았다 (p<0.049). Acetate 조성 비율은 다른 종류의 사일리지에 비해 수단그라스 사일리지 급여 1시간 (p<0.018), 3시간 (p<0.004) 및 6시간 (p<0.019) 후에 채취한 반추위액에서 높은 수준을 보였으며, propionate 조성 비율은 사일리지 급여 후 6시간까지 호밀사일리지와 청보리 사일리지를 섭취한 소에서 높은 (p<0.001~p<0.042) 반면 수단그라스 사일리지를 섭취한 소에서 가장 낮았다. Butyrate 조성 비율은 사일리지 급여 후 대부분의 반추위액 채취 시간 (1~6시간)에 걸쳐 청보리 사일리지를 섭취한 소에서 가장 낮은 (p<0.007~p<0.027) 것으로 나타났다. 반추위 내 건물의 유효분해율은 호밀 사일리지에서 59.64%로 가장 높았으며, 그 다음으로 청보리 사일리지 (56.12%) 및 이탈리안 라이그라스 사일리지 (55.64%)에서 높았고 수단그라스 사일리지의 유효분해율이 54.02%로 가장 낮았다 (p<0.048). 이러한 경향은 조단백질 (p<0.014) 및 유기물 (p<0.039)의 유효분해율에서도 비슷한 것으로 조사되었다. 수단그라스 사일리지 내 건물 (p<0.032), NDF (p<0.034) 및 유기물 (p<0.041)의 전장소화율은 다른 종류의 사일리지에 비하여 현저히 낮았으나 다른 세 종류의 사일리지 간에는 차이가 없었다. 전장소화율을 이용하여 산출된 TDN 값은 호밀 사일리지가 61.1%로 가장 높았으며 (p<0.001), 청보리 사일리지, 이탈리안 라이그라스 사일리지 및 수단그라스 사일리지의 TDN 값은 각각 57.1, 57.9 및 50.7%인 것으로 조사되었다. 이상의 결과로 미루어보아 국내에서 생산, 이용되고 있는 사료작물사일리지의 성분이 VFA 조성 비율 및 소화율에 영향하는 것으로 보이며, 특히 사일리지의 NDF 함량이 소화율에 기초를 둔 TDN 값에 크게 영향하는 것으로 여겨진다. The present study was conducted with four ruminally fistulated cattle to examine the fermentation characteristics and effective degradability (ED) in the rumen, and whole tract digestibility and TDN values of various silages (whole crop rye, RS; whole crop barley, BS; Italian ryegrass, IRGS and sudangrass, SGS) which were produced in Korea. The pH of rumen fluid were not greatly affected by silage at most times after feeding but SGS decreased (p<0.049) the ruminal ammonia-N concentration as 7.28 ㎎/100 ml at 3h after feeding compared to other silages. An acetate proportion from SGS feeding was higher at 1h (p<0.018), 3h (p<0.004) and 6h (p<0.019) after feeding than those from other silages. The propionate (C₂) proportion, however, was greater (p<0.001∼p<0.042) for the RS and BS than the corresponding values of other silages up to 6h after feeding while the lowest C2 proportion was observed from SGS. The butyrate proportion was lowest (p<0.007∼p<0.027) in the cattle fed BS at 1∼6h after feeding among silages. An ED of dry matter (DM) in the rumen was highest (P<0.048) for RS as 59.64%, and was reduced in the order of BS (56.12%), IRGS (55.64%) and SGS (54.02%). Similar tendency was observed in the EDs of crude protein (p<0.014) and organic matter (OM, p<0.039). The whole tract digestibility of DM (p<0.032), neutral detergent fiber (NDF, p<0.034) and OM (p<0.041) of SGS was greatly reduced. The TDN value based on whole tract digestibility of silages was highest for RS as 61.1%, and TDN values of BS, IRGS and SGS were 57.1%, 57.9% and 50.7%, respectively. Based on the results obtained from the present study, components in various whole crop silages produced in Korea might affect the ruminal VFA proportion, and especially, NDF content could greatly influence on the TDN value of silage calculated based on the NDF content.

반추가축영양 : 발효 팽이버섯폐배지의 볏짚 대체 급여가 사료의 면양 체내 이용성 및 거세한우의 성장에 미치는 효과

지병주 ( Byung Ju Ji ),최성호 ( Seong Ho Choi ),송만강 ( Man Kang Song ),Jugdder Shinekhuu,Guang Lin Jin 한국동물자원과학회 ( 구 한국축산학회 ) 2009 한국축산학회지 Vol.51 No.3

효과적인 자동 미생물 동정기기용 Card Panel 선택을 위한 Acinetobacter baumannii와 유사 그람 음성 세균에 대한 선별검사법 개발

지병주(Byung-Joo Ji),유성률(Sung Ryul Yu),우현준(Hyun Jun Woo) 한국보건기초의학회 2022 한국보건기초의학회지 Vol.15 No.1

Gram-negative bacteria that have a similar colonial appearance to Acinetobacter baumannii are often difficult to select for antibiotic susceptibility test card for fermentative and non-fermentative bacteria used in Vitek2 automatic microorganism identification devices. As a result of card-mismatching, the economic and time loss occurs continuously. In the current study, it has been found that oxidase positive reaction was negatively changed when A. baumannii suspension was reacted with 1% oxidase reagent and human serum. For that reason, it has been examined whether this reaction could be used as a differentiating test for A. baumannii from other gram-negative strains with similar characteristics of colonies. Pre-treated suspensions with heat, protease inhibitor, and dithiothreitol were showed that the induction of reductive reaction was due to the action of A. baumannii exoenzyme. In order to investigate the clinical applicability of this method, a total of 340 isolates were assayed for the validation of our reagent. The analysis result of kappa coefficient of the automatic microorganism identification devices and our reagent was 0.96, indicating a high agreement between the two methods. The results of this study showed that the screening test using our reagent was able to accurately select A. baumannii compared to the current work type, which relies on the subjective screening of gram-negative bacteria with similar appearance of colonies. The reagent that we invent could be contributed to improving the quality of identification of microorganisms especially for A. baumannii.

k - Casein , GMP , Sialic Acid 가 한우송아지 Rotavirus 와 제주도 Bovine Rotavirus ( JBR ) 의 MA - 104 세포감염에 미치는 영향

유제현,김종헌,유대환,신원선,지병주,송진욱,김세민,박범석 한국축산식품학회 2002 한국축산식품학회지 Vol.22 No.3

This study was conducted to investigate inhibitory effects of κ-casein, GMP and sialic acid addition on the infection of MA-104 cells by S97(Korean native cattle rotavirus) and JBR(Jeju island bovine rotavirus). MA-104 cells on incomplete M199 were infected with domestically separated S97 and JBR, activated by incubating at 37℃ for 6 days, and analyzed for the titer of rotavirus. κ-casein, GMP and sialic acid added MA-104 culture infected by activated S97 and JBR were incubated for 15 hours and stained by the AEC stainning method. The number of infected cells were counted on microscope. The titer of S97 and JBR was 2.5×10exp(7) and 2.0×10exp(6) PFU/ml, respectively. The inhibition level against cell infection by S97 was 97.4% for 2000μM of κ-casein and 97.44% for 2000μM of GMP. The inhibition level against cell infection by JBR was 99.52% for 2000μM of κ-casein and 99.78% for 2000μM of GMP. The inhibition level against cell infection by S97 and JBR was 3.85 and 3.63% for 2000μM of sialic acid, respectively. The high inhibitory effects (over 97%) of κ-casein and GMP against infection of MA-104 cells with S97 and JBR indicated great potentials for the use of κ-casein and GMP in the treatment of calf or infant caused by rotavirus.

사료작물 사일리지의 단백질 분획 및 Borate-phosphate Buffer 추출이 In vitro 발효성상, Gas 발생 그리고 분해율에 미치는 효과

김광림 ( Guang Lin Jin ),지병주 ( Byung Ju Ji ),오영균 ( Young Kyoon Oh ),홍성구 ( Seong Ku Hong ),송만강 ( Man Kang Song ),Judder Shinekhuu,Xiang Zi Li 한국동물자원과학회 2009 한국축산학회지 Vol.51 No.5

일라이트 형성과정 탐구를 통한 양이온 치환 능력 향상에 관한 연구

이진솔(Jin-Sol Lee),지병주(Byeong-Ju Ji),김진욱(Jin-wook Kim) 대한지질학회 2015 대한지질학회 학술대회 Vol.2015 No.10

모넨신 첨가 수준이 반추위 미생물에 의한 발효성상, 메탄발생량 및 건물 분해율에 미치는 영향

Guang-Lin, Jin,지병주,Xiang-Zi, Li,Judder Shinekhuu,송만강 충북대학교 동물생명과학연구소 2008 동물생명과학연구 Vol.1 No.-

본 시험은 조사료와 농후사료의 비율 및 모넨신 첨가량이 반추위 미생물의 건물 분해율, 메탄 발생량 및 배양액의 발효성상에 미치는 영향을 평가하고자 in vitro 방법으로 실시되었다. 첨가사료의 조사료 및 농후사료의 비율은 각각 8:2, 5:5 및 2:8 로 하였으며, 모넨신의 첨가량은 0, 10, 30 및 50ppm/150ml의 수준으로 하였다. 배양액은 100ml의 반추위액과 50ml의 인공타액을 혼합하여 총 150ml로 배양하였다. 여기에 기질과 모넨신을 첨가하여 39℃에서 24시간동안 혐기적으로 배양하였다. 24시간 배양하였을 때, 건물 분해율은 농후사료 첨가수준이 증가됨에 따라 증가하였으나(P<0.001), 각 농후사료와 조사료의 첨가수준 내에서는 모넨신 첨가 및 첨가수준이 건물분해에는 영향하지 않았다. 배양 시작 3시간 후 부터 배양액 내의 pH는 농후사료 첨가수준이 증가함에 따라 뚜렷하게 낮아졌다 (P<0.001). 배양 6시간 경과하였을 때, 모넨신 첨가량이 증가됨에 따라 배양액의 pH는 증가되었다 (P<0.05). 총가스 발생량은 농후사료 첨가수준이 증가될수록 많아졌으며 (P<0.001), 이러한 현상은 전체배양기간 동안 동일하였다 (P<0.01). 메탄발생량은 총 가스 발생량과 비슷한 경향이었는데, 배양 후 9시간 경과하였을 때부터는 모넨신 첨가량이 많은 처리구에서의 메탄발생량이 현저하게 감소되었다 (P<0.01). An in vitro study was conducted to examine the effect of addition level of monensin on fermentation characteristics, methane production and dry matter degradability by mixed rumen microbes. Four levels(0ppm, 10ppm, 30ppm and 50ppm, w/v) of the monensin were added to the mixed solution of strained rumen fluid with artificial saliva(2:1, v/v) and 1,5g substrate (mixture of 80% concentrate and 20% forage) in nylon bag was placed in 150ml incubation solution, and incubated anaerobically for 24hours at 39℃. pH in incubation solution increased up to 1h incubation but decreased with incubation time thereafter for all the treatments. Ammonia-N concentration increased with time for all the treatments. However, pH and ammonia concentration were not influenced by added monensin levels. Total VFA concentration increased (P<0.01) with the decreasing monensin level at 3 and 6h incubations, although there was no significant difference between treatments at 24h incubations. The propionate proportion increased (P<0.05), but acetate and butyrate decreased (P<0.01) with monensin at 3 and 6h incubations. C₂2/C₃ ratio was decreased (P<0.05) by increasing the monensin levels at 3 and 6h incubations. The methane production decreased (P<0.05) as the monensin levels increased at 24h incubations. Dry matter degradation of the substrate was not affected by the monensin addition.

대망에서 발견된 염전 분리된 난소낭성 기형종 1 예

정기성,정정은,이대원,엄상탁,전현욱,강성수,지병주 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.9

While ovarian cystic teratoma is one of the most common ovarian neoplasms, benign cystic teratoma of the omentum is a rare tumor. A 39 years old women was admitted for lower abdominal pain. Abdominal and pelvic examination revealed a goose egg sized mass in the left side of cul de sac. Ultrasonography showed 5×7 cm sized, ill-defined mixed echogenecity mass in cul de sac. At laparotomy, a 6.5×4.5×2 cm mass was found in the pelvic cavity with relation to right ovary. A 6.8×5×3.5 cm sized mass was found in the pelvic cavity, attached to the greater omentum with adhesion to the left side of cul de sac. Pathologic examination revealed typical findings for cystic teratoma in both sided lesions. We recently experienced a case which suggest mechanical separation of dermoid cyst from ovary by torsion and reported here with brief review with literautres.

임신중 수술처치한 자궁부속기 종괴의 임상적 , 병리학적 그리고 예후적 특징

이현주 ( Hyeon Ju Lee ),정기성 ( Ki Sung Chung ),지병주 ( Byung Ju Ji ),정희웅 ( Hee Woong Jeong ),이용호 ( Young Ho Lee ),엄상탁 ( Sang Tak Eum ) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.7

N/A Objective :The purpose of this study was to evaluate retrospective data concerning patients with adnexal masses that were managed surgically during pregnancy and their effect on fetal outcome. Methods :Data were reviewed concerning pregnant women who requited surgery at our hospital from January 1991 to December 2000 for an adnexal mass. Results :In the recent 10 years at our hospital a total of 47 pregnant women aged 27.9±3.6 years were diagnosed with adnexal masses that required surgery. The masses were removed at 12.3±3.7 weeks of gestation and maximum diameter was 8.4±2.3 cm. The pathologic features of the 47 lesions were as follows : 17 mature cystic teratomas, 14 epithelial origins (12 serous cystadenoma, 2 mucinous cystadenoma), 9 functional cysts, 4 endometriotic cysts, 2 paraovarian cysts, 1 malignant neoplasm. Of the 41 patients for whom the outcome of pregnancy was available, 2 (4.2%) gave preterm birth befare 37 weeks, while 4 (8.5%) experienced spontaneous abortion. There were not any perinatal death for the 43 infants. Conclusion .Although our studies are smaller for confinnation, so larger studies are required, but our results suggest that an adnexal mass may be associated with an adverse fetal outcome. Surgical intervention at<22 weeks of gestatian might not have been related to the advem fetal outcomes.