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인삼 사포닌의 생화학적 연구 ( Ⅹ ) 한국산 인삼 사포닌의 지질대사에 미치는 영향
주충노,이희봉,김두식 ( Chung No Joo,Hee Bong Lee,Doo Sik Kim ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.2
It was attempted to observe the effects of ginseng saponins, one of the major components of Korean ginseng roots, on fatty acid oxidation and synthesis in vitro as well as on lipid metabolism in vivo and the following results were obtained. 1. It was found that the ginseng saponins stimulated significantly both fatty acid oxidation by rat hepatic mitochondrial preparation and synthesis by the cytosol fraction. 2. Distribution of radioactivities of lipids in liver, brain, adipose tissue and blood serum of albino rats administered with the ginseng saponins prior to acetate -1, 2-^(14)C injection intraperitoneally have been investigated. 3. The high radioactivities of lipid in the above tissues of test group except blood serum appeared at about 30 min. to 60 min. after acetate injection while those of control group was observed at about two hours after suggesting that the ginseng saponins stimulated the biosynthesis of lipids in the above tissues. 4. Under the experimental conditions in this study, it was realized that biological half lives of hepatic cholesterol and fatty acid of test rat were found to be 19min. and 44min. while those of the corresponding lipids of control rat were 30min. and 70min. respectively suggesting that the ginseng saponins might stimulate the metabolism, both biosynthesis and degradation of cholesterol and fatty acid of the liver of this animal. 5. In the brain, the highest specific radioactivities of cholesterol and fatty acid of test group were observed at 15min. and 30min. after acetate-1, 2-^(14)C injection while those of the corresponding lipids of control group were found at 216min. and 156min. after the injection respectively suggesting that the ginseng saponins stimulated greatly the metabolism of brain lipids. 6. In the adipose tissue, the highest specific radioactivity of fatty acid of test group was observed at 61min. after acetate -1, 2-^(14)C injection while that of the control group appeared at 124 min. after the injection suggesting that the ginseng saponin stimulated Carbon-14 incorporation into fatty acid in this tissue. 7. It appeared that the high radioactivity of blood serum lipid of control group was observed at 216min. after acetate-1, 2-^(14)C injection but the radioactivity peak of test group was observed at 79 minutes after the injection of 1, 2-^(14)C-acetate signifying that the ginseng saponin affected not only lipid metabolism but also lipid transport in the animal body.
인삼 사포닌의 생화학적 연구 ( Ⅵ ) Glutamate Dehydrogenase 및 Glutamate Transaminase 에 미치는 영향
주충노,오종환,노수진 ( Chung No Joo,Jong Whan Oh,Soo Jin No ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.1
It was attempted in this experiment to observe the effect of ginseng saponins, one of the major components of Panax aoiuseng C.A. Meyer roots, on purified bovine hepatic glutamate dehydrogenase (GIDH) in vitro. It was found previously that the chicken`s hepatic mitochondrial glutamate dehydrogenase was activated significantly in the presense of saponins and that the maximum activity of GLDH was observed when the concentration of the saponins in the assay mixture was 0.1%. In this experiment the activity of purified bovine hepatic glutamate dehydrogenase in the presence o the saponins was measured and compared with that of control. It was realized that the activity of GLDH was the highest when the concentrarion of the saponins in the assay mixture was 10^(-5)∼10^(-6)%, the activity being about 1.5 times that of control. In the presence of the excess saponins above 10^(-4)% in the assay mixture the GLDH was inhibited gradually. It was also found that the ginseng saponins did not affect ADP activation of GLDH or GTP inhibition of GLDH signifying that the saponins did not interfere with GLDH regulation of the metabolism involved. Furthermore, the ginseng saponins were realized to activate both the glultamatepyruvate transaminase and glutamate-oxaloacetate transaminase but the degree of activation was not significant when compared with that of GLDH.