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구기자(Lycii fructus) 추출물이 미생물 생육에 미치는 영향
주인선(In-Sun Joo),성창근(Chang-Keun Sung),오만진(Manjeen Oh),김찬조(Chan-Jeo Kim) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.4
구기자 추출물이 S. cerevisiae D71과 L. casei KCTC3165, 그리고 E. coli DH5균의 생육에 미치는 영향을 검토하였다. 그 결과 구기자 추출물은 S. cerevisiae D71과 L. casei KCTC 3165의 생육을 증가시켰으며 E. coli DH5에 대하여는 약간의 생육저해가 있었다. 구기자 methanol 추출물을 첨가하여 미생물의 생육을 측정하였을 때 L. casei KCTC 3165와 S. cerevisiae D71는 각각 1.0%와 1.5% 첨가구에서 가장 높은 생육촉진효과를 보였다. 즉 구기자 추출물은 대장균의 생육은 저해하였으나, 인체에 유익한 균으로 알려진 젖산균에 대하여는 생육을 촉진하는 특성이 있었다. 한편, E. coli DH5는 구기자즙 10%, 그리고 methanol, chloroform 추출물 각각 1.0% 첨가시 형태적 이상을 일으켜 길이가 8배 이상이 길어졌다. 구기자의 methanol 추출물 중 회분성분이 E. coli DH5균에 이상현상의 원인이 되었음을 확인하였다. 또한 이들 중 Na^+, K^+ 및 Na^+와 K^+ 혼합물이 0.135% 이상에서 균의 형태가 길어지는 이상현상이 있었다. This study was performed to investigate the effects of Lycii fructus extracts on the growth and physiology of S. cerevisae D71, L. casei KCTC 3165, and E. coli DH5. When Lycii fructus was added into solid culture media, the growth of S. cerevisea D71 and L. casei KCTC 3165 was increased, whereas that of E. coli DH5 was somewhat decreased. The growth of S. cerevisiea D71 and L. casei KCTC 3165 were much promoted in the culture media including methanol extract of 1.0% and 1.5%, respectively. E. coli DH5 was changed its morphology not only in 10% Lycii fructus juice but also 1.0% methanol and chloroform extract of Lycii fructus. Its size in those growth condition was eight times longer than that of the normal E. coli DH5. It was elucidated that elongation phenomenan of E. coli DH5 was also appeared by adding 0.135% of Na^+, K^+, and the mixture of Na^+ and K^+.
Detection of CTX-M Type ESBL Producing Salmonella in Retail Meat in Korea
김영훈,주인선,김윤정,오미현,조준일,한민경,김순한,Moon Tae Wha,Park Kun Sang 한국식품위생안전성학회 2014 한국식품위생안전성학회지 Vol.29 No.1
This study was performed to evaluate antimicrobial resistance of food-borne pathogens isolatedfrom retail meat in Korea. A total of 157 samples of beef, pork, and chicken were collected and analyzed for E. coli,Salmonella, Campylobacter. Resistances to tetracycline were declined in accord with reduced usage of tetracycline inlive stock production. E. coli stains from chicken meat had higher multi-drug resistance ratio than strains from othermeat. One extended spectrum beta lactamase (ESBL) producing E. coli and two ESBL producing Salmonella wereidentified in this study. ESBL producing Salmonella strains were confirmed to carry CTX-M-1 type genes.
조준일,주인선,박건상,한민경,손나리,정숙진,허진,김윤정,오미현,김순한,이순호 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1
Pathogenic Escherichia coli (PEc) is a leadingcause of both foodborne and waterborne illness. In September2012, a major foodborne outbreak with PEc occurred,affecting approximately 1,200 students and food handlersfrom 7 schools in Gyeonggi province, South Korea causedby contaminated kimchi. For detection of PEc in kimchi,real-time polymerase chain reaction (RT-PCR) and traditionalculture methods were used. EAEC and ETEC genotypeswere identified in samples from individuals with the illnessand in kimchi using conventional PCR. Bacteria in stoolsamples were genetically similar to bacteria from kimchi(98% homology). PEc from kimchi was identified as thecausative agent of a foodborne outbreak in South Korea. Asignificant link between kimchi and individuals with foodborneillnesses after consuming kimchi was demonstrated.
조준일,주인선,최준혁,정경훈,최은정,손나리,한민경,정숙진,이순호,황인균 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.3
Methicillin-resistant Staphylococcus aureus(MRSA) in raw meat and fish samples in Korea wasinvestigated. A total of 209 samples was analyzed. Antimicrobial disk susceptibility testing with oxacillin(OX) was used to detect MRSA in 74 S. aureus isolates(35.4%), 7 of which showed resistance towards OX. ThemecA gene was identified in the 7 isolates with OXresistance and was expressed in all 7 isolates. Pulsed-fieldgel electrophoresis was used to analyze genetic homologiesamong the 7 MRSA isolates. No genetic correlations weredetected among the isolates.
Prevalence and Characterization of Foodborne Bacteria from Meat Products in Korea
조준일,주인선,최준혁,정경훈,최은정,이순호,황인균 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.5
In this study, the distribution of Salmonella spp., Escherichia coli, Staphylococcus aureus, Campylobacter jejuni, and Vibrio parahaemolyticus in raw meat products in Korea were investigated. A total of 155 meat products consisting of 52 beef, 62 pork, and 41 chicken were purchased randomly from 41 stores located in 5 different Korean provinces. E. coli and S. aureus were detected in 37.4 and 33.5% of the samples. Salmonella spp., C. jejuni,and V. parahaemolyticus were not detected. More than 30% of S. aureus were found to be enterotoxin producers and these organisms primarily possessed type A toxin genes. Conversely, verocytotoxin producing E. coli were not detected. Taken together, these results indicate that consumption of raw meat products may pose a risk of foodborne disease and that good hygienic practices should be required to ensure public health.
조준일,주인선,최준혁,정경훈,최은정,한민경,정숙진,손나리,이순호,황인균 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.1
From February to October 2011, 209 samples of retail raw meat and fishery products were randomly obtained from 41 grocery stores in Korea and cultured for the presence of Enterococcus spp. Ninety-six enterococcal isolates were recovered from 76 samples, with contamination rates ranging from 18.5% in fishery product samples to 43.9% in chicken samples. Antimicrobial disk susceptibility testing was conducted according to the Clinical and Laboratory Standards Institute protocol. The antibiotic resistance rates of the 96 enterococci isolates were as follows: tetracycline (TE) 77.1%; erythromycin (E) 50%;rifampin (RD) 44.8%; and vancomycin (VAN) 9.4%. Disk diffusion showed that 9 isolates were resistant to vancomycin. The minimum inhibitory concentration (MIC) was ≥32 μg/mL for all 9 isolates, and all were resistant to vancomycin. Among the 9 vancomycin-resistant enterococci (VRE)identified, the vanA gene was carried by 1 Enterococcus durans strain and the vanB gene was carried by 2Enterococcus faecium, and 1 Enterococcus hirae strains. Further genotyping of the VRE isolates using pulsed-field gel electrophoresis (PFGE) revealed extensive heterogeneity. The VRE were recovered from various animal sources with a particularly high prevalence of E. faecium found in fishery products and chicken.
Predictive model of Staphylococcus aureus growth on egg products
최원석,손나리,주인선,한정아,곽효선,홍진환,서수환 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3
Egg products are widely consumed in Korea andcontinue to be associated with risks of Staphylococcusaureus-induced food poisoning. This prompted the developmentof predictive mathematical models to understandgrowth kinetics of S. aureus in egg products in order toimprove the production of domestic food items. Eggproducts were inoculated with S. aureus and observe S. aureus growth. The growth kinetics of S. aureus was usedto calculate lag-phase duration (LPD) and maximumspecific growth rate (lmax) using Baranyi model as theprimary growth model. The secondary models providedpredicted values for the temperature changes and werecreated using the polynomial equation for LPD and asquare root model for lmax. In addition, root mean squareerrors (RMSE) were analyzed to evaluate the suitability ofthe mathematical models. The developed models demonstrated0.16–0.27 RMSE, suggesting that models properlyrepresented the actual growth of S. aureus in egg products.
Ultrafast Real-time PCR법을 이용한 살모넬라의 신속 검출
김석환,이유시,주인선,곽효선,정경태,김순한 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.1
Salmonella continue to be a major cause of food poisoning worldwide. The rapid detection method of food-borne Salmonella is an important food safety tool. A real-time polymerase chain reaction (PCR) has been used as a rapid method for the detection of pathogens. It has been recently reported that NBS LabChip real-time PCR is a novel, ultrafast, and chip-type-convenient real-time PCR system. We developed the assay method based on NBS Lab- Chip real-time PCR for the rapid detection of Salmonella, which its reaction time was within 20 minutes. Two target genes (invA and stn) were selected to design target specific primers and probes. The new method was validated by checking specificity and sensitivity (limit of detection). This study included forty-two target and twenty-one non-target strains to assess the specificity. This assay was able to identify the 42 Salmonella strains correctly. The limit of detection (LOD) was 101 copies/μL in Salmonella genomes DNA, while LOD incubated for 4 hr in the inoculated sausage sample ranged from 101 CFU/g to 102 CFU/g as an inoculated cell count. The assay developed in this study could be applied for the investigation of food poisoning pathogens.