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MALDI-TOF MS를 이용한 효모에서의 황화수소 생성 단백질의 동정
조현남,판루안,유동찬,양선아,이인선,김재형,백효현,지광환 한국생물공학회 2008 KSBB Journal Vol.23 No.5
Hydrogen sulfide (H2S) is a by-product of metabolism of amino acids including sulfur and alcoholic fermentation, it is generally thought of in terms of a poisonous gas. Though H2S can have a negative impact on the perceived quality of fermented drinks due to an undesirable aroma, it plays prominent roles as a neuromodulator in the mammalian brain as well as a smooth muscle relaxant. Nowadays studies on the proteins which produce H2S are carried out in various fields such as structure, function, and metabolism. Here we propose to develop a simple and rapid H2S forming assay method, which will lead to speed up preparing the H2S forming proteins for identification by MALDI-TOF MS analysis. We detected three kinds of proteins which produce H2S in the crude extract of Saccharomyces cerevisiae. Those proteins were cystathionie β-synthase, O-acetylserine sulfhydrylase, and cystathionine γ-lyase.