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400 kHz 페라이트 유도 결합 플라즈마를 위한 임피던스 매칭 네트워크
조성원,방진영,이영광,정진욱,Cho, Sung-Won,Bang, Jin-Young,Lee, Young-Kwang,Chung, Chin-Wook 한국반도체디스플레이기술학회 2007 반도체디스플레이기술학회지 Vol.6 No.2
Low frequency (400 kHz) Ferrite ICP has been proposed for large area processing. Because the coupling coefficient is close to 1, the transformer matching can be adapted to Ferrite ICP. The transformer matching system is simple. In this paper the new matching system by controlling the turns of transformer using relays is proposed for impedance matching. We developed a simple matching system and characterized it when the turns were changed. It was observed that the 2-channel relay is available for transformer matching.
Zebrafish 동물모델에서 human HtrA2의 expression system 정립에 관한 연구
조성원,박효진,김구영,남민경,김호영,고인호,김철희,임향숙,Cho, Sung-Won,Park, Hyo-Jin,Kim, Goo-Young,Nam, Min-Kyung,Kim, Ho-Young,Ko, In-Ho,Kim, Cheol-Hee,Rhim, Hyang-Shuk 한국생명과학회 2006 생명과학회지 Vol.16 No.4
Mitochondrial serine protease로 알려진 human HtrA2 (hHtrA2)는 apoptosis 유도 과정에서 중요한 역할을 담당하고 있을 뿐만 아니라 hHtrA2가 motor neuron degeneration과 관련이 있다는 최근 연구 결과가 있으나, hHtrA2의 생리적 기능은 아직 명확하게 밝혀져 있지 않다. 이와 같이 생체내에서 필수적인 업무를 담당하는 hHtrA2의 기능을 심도 있게 연구하기 위해서는 적절한 동물모델 시스템이 필요하나 이에 대한 연구도 미흡한 실정이다. 따라서 본 연구에서는 hHtrA2의 기능 분석을 위한 기본적인 실험으로 zebrafish라는 동물모델을 선택하여 hHtrA2의 발현 시스템을 정립하였다. 먼저 zebrafish에 hHtrA2를 발현시키기 위하여 zebrafish에서 일반적으로 사용되는 발현 시스템인 pCS2+ vector에 hHtrA2와 GFP를 cloning하고 plasmid를 HEK293 cell에 transfection한 후, hHtrA2-GFP fusion 단백질의 발현을 immunoblot과 immunofluorescence staining assay로 확인한 바 약 64 kDa의 hHtrA2 단백질의 발현을 확인할 수 있었다. Zebrafish에서 hHtrA2-GFP fusion 단백질의 발현양상은 immunofluorescence microscope으로 확인하였다. hHtrA2-GFP DNA와 mRNA를 zebrafish embyro에 microinjection하여 두 가지 component의 발현을 비교 분석한 결과, DNA는 dot 형태로 mRNA는 몸 전체에 퍼져보이는 형태로 발현 양상의 차이는 있었으나 둘 다 zebrafish embryo에서 잘 발현되는 것을 알 수 있다. 다음 DNA를 주 component로 microinjection하여 zebrafish embryo에서 발현을 확인한 결과 hHtrA2는 72 hpf 까지 발현이 지속되는 것을 확인하였다. 본 연구에서 정립한 hHtrA2의 zebrafish 발현 조건은 앞으로 zebrafish에서 hHtrA2의 생리적 기능을 심도있고 정확하게 연구하는 데 있어 기본적인 자료로 활용 할 수 있을 것이다. HtrA2/Omi, a mitochondrial trypsin-like serine protease, is pivotal in regulating apoptotic cell death. Several lines of recent evidence suggest that HtrA2 is associated with the pathogenesis of neurodegenerative disorders; however, the physiological function of HtrA2 still remains elusive. For studying physiological function of HtrA2 in depth, it is necessary to develop a suitable expression system in the model animal. We therefore utilized the zebrafish as a model animal to establish expression of human HtrA2 (hHtrA2) in vivo. For expression of mature HtrA2 as GFP fusion in zebrafish embryos, the HtrA2 (WT) or (S306A) cDNAs with the C-terminal GFP tag were inserted into the pCS2+ plasmid. Expression patterns of HtrA2 in HEK293 cells were first monitored by immunofluorescence staining and immunoblot assays, showing approximately 64 kDa of the HtrA2-GFP fusion proteins. Subsequently, the hHtrA2 plasmid DNA or in vitro transcribed mRNA was microinjected into zebrafish embryos. The expression patterns of HtrA2 in Zebrafish embryos were monitored by GFP fluorescence in 24 hours-post-fertilization (hpf). Although expression patterns of HtrA2-GFP in developing embryos were different between the injected DNA and mRNA, both nucleic acids revealed good expression levels to further study the physiological role of HtrA2 in vivo. This study provides a suitable condition for expressing hHtrA2 in the zebrafish embryos as well as a method for generating useful system to investigate physiological properties of the specific human genes.
위장관 ( 胃腸管 ) : 당뇨병성 자율신경 장애증 환자에서 복부 초음파 촬영술을 이용한 위내용물 배출시간 ( Gastric Emptying Time ) 의 측정
조성원(Sung Won Cho),심찬섭(Chan Sup Shim),김진홍(Jin Hong Kim),이문성(Moon Sung Lee),조주영(Joo Young Cho),김대수(Dae Soo Kim) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.3
N/A To determine the validity of the ultrasonographic methods for evaluation of gastric emptying, a total of 41 subjects were examined including 20 normal subjects and 21 patients with diabetic autonomic neuropathy (DAN). Gastric emptying times of liquid meals (500ml isotonic saline contained 37MBq of DTPA marked with 99mTc) were determined in all subjects by three different methods; Method A: measuring the changes in sums of anteroposterior and laterolateral diameters with a single ultrasonographic scan performed at the epigastrium, Method B: measuring the changes in the ultrasonographic cross sectional areas at the same sections, and method C: measuring the changes in gastric isotope counts by scintigraphy. In a subgroup of 16 subjects (9 normal, 7 DAN) gastric emptying times of solid meals (717Kcal contained egg white with 37MBq of sulfur colloid marked with 99m Tc) was considered by the same three methods. The results show that 1) 50%, 80% and 95% gastric emptying of liquid and 50%, 80% emptying of solid meals obtained by Method A, B or C in DAN were significantly prolonged compaired to normal subjects (p<0.01), and 2) the values obtainhd by Method A or B do not differ from the ones obtained by Method C. These suggest that DAN had delayed gastric emptying of the liquid and solid meals, and the determination of gastric diameters or areas in a single section of stomach by ultrasonography is a valid method for evaluating gastric emptying in DAN as well as nomal subjects.
상온양생에 의한 하이브리드 섬유를 혼입한 50MPa급 3성분계 무시멘트 모르타르 강도발현
조성원 ( Cho Sung-won ),조성은 ( Cho Sung-eun ),김영수 ( Kim Young-su ) 한국건축시공학회 2020 한국건축시공학회 학술발표대회 논문집 Vol.20 No.1
CO2 emissions are caused by cement manufacturing process. To solve this problem construction industry are using industrial by-products to replace cement. In this study, three different industrial by products were used and mixed with hybrid fibers to enhance bond strength. As the result, Regardless of the mixing rate of silica fume, the compressive strength of the ternary non cent mortar was higher than that of OPC and binary. And mixed hybrid fibers cured by room temperature compressive strength were 23% higher than those without hybrid fibers.
어린이집의 연간 실내 미세먼지 농도 분포 및 유입량과 발생량에 관한 연구
조성원(Cho, Sung-Won),박준석(Park, Jun-Seok) 대한건축학회 2020 대한건축학회 학술발표대회 논문집 Vol.40 No.2
As the day of the high concentration of particle matters in the atmosphere is increasing, the Korea government recommends to refrain from outdoor activities and stay indoors. Thus, as the amount of time spent living indoors increases, interest in the indoor air quality pollution of particles in the atmosphere is rapidly increasing. Especially, the daycare center is a facility where infants and children staying for long time, and is more vulnerable to diseases caused by particles than adults, so the Korean government is strengthening standards for indoor particle concentration in daycare centers. Therefore, this study aims to analyze whether the annual indoor particle concentration in daycare center satisfies the maintenance guideline.