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放射性沃度(^131 I)가 생쥐 腸間膜 肥滿細胞에 미치는 영향
趙士先,尹錫鳳 서울대학교 1974 서울대학교 論文集 Vol.24 No.-
The study was carried out to observe the internal effects of radioactive iodine on the mesenteric mast cells of mice. The mice, weighing about 25g, were given a single does of 3μc ^131I/g body weight intraperitoneally. The mice were anesthetized with ether and sacrificed by opening the peritoneal cavity after ^131I injection with the time intervals of 1, 6, 12, 24, 48, 72 and 96 hours. The mesenteries were, then, removed immediately and fixed in absolute alcohol and stained with Pugh's solution. The average number and the morphological alternation of the mast cells were observed from 10 microscopic fields with 450 magnification. The results obtained through the experiment were summarized as follows. 1. The number of mast cell decreased gradually by the time period from 1 hour up to 12-24 hours, and then, recoverd with the time. The time period required for normal state was 96 hours after ^131I injection. 2. The morphological alternations by the injection of ^131I appeared as the decreasing stainability, vacuolation, and disruption of the cells, and the most severe changes were observed at 24-48 hours after ^131I injection.
생쥐의 난소의 $\beta$-Endorphin에 대한 면역조직화학적 동정
조사선,이영기,김경진,윤용달,이정주,조완규 한국통합생물학회 1990 동물학회지 Vol.33 No.2
The present study, using immunohistochemical procedure, was carried out to determine the localization of immunostainable $\beta$-endorphin cells in the mouse ovarian tissues. Mature female mice were perfused with 4% neutral buffered paraformaldehyde under anesthesia and then frozen-sections were immunostained with anti $\beta$-endorphin antiserum according to ABC technique. Immunoreactive $\beta$-endorphin was found in the luteal cells of corpus lutea, but not in the thecal cells. More strong immunostaining signak were observed in large corpus luteum, in particular, the regressing luteal cells. Primary and secondary follicles did not show any immunoreactivity of $\beta$-endorphin, but granulosa cells lining the antral cavity of large antral follicles contained immunoreactive $\beta$-endorphin. 면역조직화학적 방법을 이용하여 생쥐 난소에서 $\beta$-endorphin이 생성되는 부위를 조사하기 위하여 본 실험을 행하였다. 성숙한 생쥐를 4% neutral buffered paraformaldehyde로 관류고정하였으며 난소를 적출한 후 cryostat로 절편을 만들어 avidin-biotin complex(ABC)를 이용하여 면역색을 하였다. $\beta$-endorphin에 대한 항체반응은 주로 황체에서 일어났으며, theac interna와 theca externa에서는 반응이 나타나지 않았다. 황체에서의 염색 양상은 퇴화가 많이 진행된 황체세포에서 보다 강한 연색반응을 관찰할 수 있었다. 때로 황체세포에서 유래된 것으로 보이는 간질세포에서도 양성반응을 관찰할 수 있었다. 이외에도 large antral follicle의 여포강에 인접한 과립세포에서 약한 반응을 보여주었으나, 제 1차 여포에서는 양성반응이 나타나지 않았다.