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B16 Melanoma 세포에서 Chitosan Oligosaccharide에 의한 Melanin 생성과 Tyrosinase 활성 및 발현과의 관계
조남영 대한피부미용학회 2004 대한피부미용학회지 Vol.2 No.1
To investigate the effect of chitosn oligosaccharide on cosmetic and skin care, we measured tyrosinase activity and melanin production in B16 melanoma cells, and elastase and hyaluronidase activity. Chitosan oligosaccharide itself did not have any anti-oxidant activity in DPPH radical scavenging, and did not affect the proliferation of B16 melanoma cells. Chitosan oligosaccharide dose-depednetly increased melanin production in the absence or presence of MSH. However, chitosan oligosaccharide did not have any influence on the tyrosinase activity and tyrosinase expression in B16 melanoma cells. These results suggest that chitosan oligosaccharide-induced melanin production may be independent on tyrosinase in B16 melanoma cells. On the other hand, chitosan oligosaccharide increased neutrophil elastase activity but decreased hyaluronidase activity in a dose-dependent manner. From the above results, chitosan oligosaccharide dose-dependently appears to increase melanin production in B16 melanoma cells and inhibit hyaruronidase activity, suggesting that chitosan oligosaccharide may be used as sun-tan agent and water conservative agent.
논문집 초록 : 학술발표일정 ; 3. 포스터발표 : 도금폐수의 섬유활성탄 흡착처리에 관한 연구
조남영,김백영,최태규 한국환경관리학회 2003 하계학술연구발표회 Vol.2003 No.-
도금폐수는 고농도 도금폐액과 저농도 도금세정폐수로 이루어져 있는데, 이중 50% 이상을 차지하고 있는 저농도 도금세정폐수만을 분리하여 섬유활성탄(ACAF)을 이용해 중금속물질 구리와 니켈을 흡착 제거하였다. 구리와 니켈의 인공폐수를 농도 25mg/l록 각각 제조하여 진탕시키며 ACAF의 중금속물질 흡착제거율을 분석한바, 4시간 경과시 최대 각각 90%로 나타났고, 단위시간에 ACAF 단위중량 g당 중금속물질 흡착량은 0.027g/g/hr로 산출되었다. 구리와 니켈 인공폐수를 혼합해 유입속도 1.2l/hr로 주입시키며 흡착제거율을 분석한바 구리는 최대 88.9%, 니켈의 경우는 84.6%로 나타났고, 현재 가동중인 도금업체 D社의 구리 도금공정 및 니켈 도금공정에서 실제로 발생되는 저농도 도금세정폐수를 혼합시켜, ACAF 흡착상을 통과한 처리수의 수질을 분석한바, 구리의 최대 흡착제거율은 86.8%, 니켈은 73.5 %로 나타났다. Plating wastewater consists of high density plating wastewater and low density plating rinse wastewater. Of these two types of wastewaters, only low density plating rinse wastewater which occupies more than 50% was separated and tested adsorption and removal of heavy metals Copper(Cu) and Nickel(Ni) utilizing Actived Carbon Adsorptive Fiber(ACAF). As the result of analysis of adsorption and removal rate of heavy metal by ACAF after producing artificial waste water of Cu and Ni at the density 25mg/l respectively and through process of agitation, each case showed maximum removal rate of 90% after 4 hours and adsorbed amount of heavy metal per unit weight(g) of ACAF per hour was calculated as 0.027g/g/hr. As the result of analysis of adsorption and removal rate through supplying mixed artificial wastewater of Cu and Ni at inflow velocity of 1.2 l/hr, Cu showed maximum removal rate of 88.9% and Ni showed maximum removal rate of 84.6%. After mixing low density plating rinse plating wastewater actually generated from Cu plating process and Ni of "D" company (Presently operating), an analysis of the quality of treated wastewater which has passed the ACAF adsorbing bed was made and as the result of analysis, the maximum adsorption removal rate of Cu was 86.8% and Ni was 73.5%.
미토콘드리아 small subunit rDNA 염기서열 분석을 통한 느타리 버섯의 동정
조남영 대전대학교 기초과학연구소 2000 自然科學 Vol.11 No.1
강경 한국 종균 배양소에서 분양받은 일곱 개의 다른 느타리버섯 류의 동정을 하기 위하여 polymerase chain reaction(PCR) 방법으로 미토콘드리아 small subunit(mt SSU) rDNA의 일부에 해당하는 부분을 증폭하고, 이 들의 염기서열을 결정하였다. GenBank의 database에 저장되어 있는 Pleurotus ostreatus mt SSU rDNA의 염기선열과 배열하여 분석한 결과 한 종류를 제외하고는 유사도가 매우 높게 나와, 형태학적 특징에 의한 분류와 일치하게 P. ostreatus로 판정할 수 있었다. 한 종류만 PCR에서 얻은 산물의 크기도 작고 염기서열의 유사도도 비교적 낮았으며, Genebank의 database에 비교한 결과 Oxyporus latematquinatus에 가장 근접하게 나타났다. 이와 더불어 염기서열 유사도를 비교하여 각 종류간 계통학적 관계를 알아보았다. To identify the seven different Pleurotus sp. strains obtained from Korean Strain Culture Company in Ganggeong, mitochondrial small sbunit (SSU) rDNAs were amplified by polymerase chain reaction(PCR). The PCR products were purified and subject to direct sequencing using automated DNA sequencer. Sequences of them were aligned with those of Pleurotus ostreatus accessed from the Gedank, and analyzed for the similarity. Results showed that six out of seven strains were closely related to P. ostreatus. One strain showed the smaller PCR product compared to the others and lower similarity to P. ostreutus. Rather, it seemed to be more related to Oxyporos latematquinatus.
혐기성 소화 슬러지의 상등수 및 침전물내의 미생물 활성에 관한 연구
조남영 건국대학교 1991 論文集 Vol.7 No.-
The decomposition of artificial sludge in batch type anaerobic digester was examined in terms of the microbial activity between supernatant and sediment. The temperature in the anaerobic digester was maintained at 35℃. The rate of seed to substrate was 2 : 3 on volume basis. The biogas production rates of the sediment was greater than that of the supernatant. The ultimate removl efficiency of organic matter was not difference between the supernatant and the sediment. It was estimated that the supernatant was able to be used for seeding sludge. Most of organic matter was decomposed during the initial step of acid fermentation predominating.
B16 Melanoma 세포에서 Chitosan Oligosaccharide가 Melanin 생성에 미치는 영향
조남영,윤미연,김경원,박영미,임혜원,이지윤,이진희,김연정,김창종 대한약학회 2003 약학회지 Vol.47 No.6
To investigate the effect of chitosan oligosaccharide on melanin synthesis, we measured tyrosinase activity and melanin production in B16 melanoma cells. Chitosan oligosacchaide itself did not have any anti-oxidant activity in DPPH radical scavenging, and did not affect the proliferation of B16 melanoma cells. Chitosan oligosaccharide dose-dependently increased melanin production in the absence or presence of MSH. However, chitosan oligosaccharide did not have any influence on the tyrosinase activity and tyrosinase expression in B16 melanoma cells. These results suggest that chitosan oligosaccharide-induced melanin production may be independent on tyrosinase activity in B16 melanoma cells. From the above results. chitosan oligosaccharide dose-dependently appears to increase melanin production in B16 melanoma cells, suggesting that chitosan oligosaccharide may be used as a tanning agent.