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각종 임상검체의 결핵균 검출 양성률과 염색 방법에 따른 비교 관찰
조금장 인제대학교 1986 仁濟醫學 Vol.7 No.1
결핵균 도말을 의뢰한 4,258건 중 균 양성 302건을 대상으로 검체별 양성률과 성별 · 연령별 균 양성 환자의 분포 상황과 함께 이들을 대상으로 배양 및 형광 염색과 Z-N염색을 동시 실시하여 진단을 위해서는 어떤 방법의 적용이 더 바람직한가를 조사하여 다음과 같은 결과를 얻었다. Even in decreasing trends of the prevalence of tuberculosis, the prevalence of bacteriologically confirmed pulmonary tuberculosis is 0.46% (estimated value, M.H.S.A., 1985) and death rate is still 31.7%(Based on vital registration, 1984) in Korea. Therefore prevention of occurrence of new patient by early detection as early as possible is one of the most important task in eradicating tuberculosis. Since Hagemann (1973) first described the fluorescence microscopy for demonstrating acid fast bacilli, there is convincing evidence that fluorescense microscopy is more sensitive than Ziehl-Neelsen method in literatures. Se author made a study to evaluate the usefulness of the fluorescent technique as a routine screening method for clinical materials suspected to contain acid fast bacilli. In this study, two smears were prepared before culture, one stained with Auramine fluorochrome procedure, and the other with conventional Ziehi-Neelsen procedure, from 4,258 specimens from various sources. Each was examined by Leitz Dialux 20 fluorescence microscope and conventional light microscope respectively. The results are summarized as follows 1.Number of specimen sources requested for AFB smear are; sputum, 3,329 cases (78.2%), bronchial washings, 268 cases (6.3%), urine, 263 cases (6.25) etc. among total 4,258 cases. 2.AFB smear-Positive rate is 7.1% (302/4,258 cases) and the rates in different specimen sources are; pus 15.4%, aspirated materials 11.9%, sputum 7.8% etc. 3.AFB smear-positive ratio in sex is 1.75:1 (M:F)-statistically significant. 4.Age distribution of AFB smear-positive rates are; 20-29 age group, 10.7%, 40-49 age group, 9.2%, 10-19 age group, 7.8%, etc. 5.Fluorescence microscopy revealed slightly higher sensitivity then Ziehl-Neelsen microscopy but the result is statistically insignificant. On the basis of this study, author recommend that the fluorochrome procedure should be used as the routine screening method for the early detection of acid-fast bacilli in various clinical specimens and the follow up study of the efficacy of antituberculous chemotherapy. But in the cases of doubtful result in fluorochrome procedure, confirmation should be made after a Ziehl-Neelsen procedure.
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여자 운동선수들의 혈중 각종 생화학 검사치의 변동에 관한 연구
백인기,조금장,정낙승,고일향,하권익 인제대학교 1990 仁濟醫學 Vol.11 No.1
여자 운동선수군들의 혈청에서 CK, HDL-C. Cholesterol. TG, LD 및 AST의 6가지 생화학 검사를 실시하여 각 운동선수군에서의 혈중 농도와 같은 연령군에 있는 대조군의 혈중 농도를 비교하여 운동이 젊은 여성군에서 각 생화학 검사의 혈중 농도치에 어떠한 영향을 미치는지 고찰하여 보았다. The relationship of exercise and serum chemistry levels on female athletes were investigated in 24 female volleybal players (V), 16 female hockey players (H) and 33 inactive female control (I) in same age group. Fasting serum Creatine kinase (CK), High density lipoprotein cholesterol (HDL-C), Total cholesterol (TC) Triglyceride (TG), Lactate dehydrogenase (LD), Aspartate aminotransferase (AST, SGOT) were measured and compared in three groups and results were summarized as follows. (1) CK levels were significantly higher in V and H players compared to I (P<0.01) and CK levels in V players were also significantly higher than ones in H players. (P<0.01) (2) CK index (CK / BW kg) showed similar results as CK levels among the groups. (3) HDL-C levels were significantly higher in V and H players compared to I (P<0.01), but were not significantly different among both athletes groups. (4) TC levels were not significantly different among three groups. (5) HDL-C index (TC / HDL-C) were significantly lower in V and H players compared to I (V ; P<001, H ; P <005), but were not significantly different among both athletes groups (6) TG levels were significantly lower in V players compared to I (P<0.01), but TG levels in H players were not significantly different compared to I. (7) LD levels were significantly higher in V and H players compared to I (V ; P<0.01, H ; P<0.05), and LD levels in V players were also significantly higher than ones in H players. (P<0.05) (8) AST levels were significantly higher In V and H players compared to I (V ; P<0.01, H , P<0.05) and AST levels in V players were also significantly higher than ones in H players (P<0.05) (9) CK index/ HDL-C index ratio were significantly higher in V and H players compared to I (P<0.01) and this ratio were also significantly higher in V players compared to H players. (P<0.05) Above results indicated that the intensity of muscular exercise in V players were more than one in H players and showed that CK. LD. HDL-C, CK index, HDL-C index and CK index/ HDL-C index ratio could be used as good indices for estimating intensity & duration of muscular exercise in athletes.
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