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유통 마른 김 (Porphyra sp)의 중금속 (Hg, Pb, Cd) 함량과 위해성 평가
손광태 ( Kwang Tae Son ),권지영 ( Ji Young Kwon ),조미라 ( Mi Ra Jo ),최우석 ( Woo Seok Choi ),강성림 ( Sung Rim Kang ),하나영 ( Na Young Ha ),신진월 ( Jin Wall Shin ),박큰바위 ( Kunbawui Park ),김지회 ( Ji Hoe Kim ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.5
To investigate heavy metals (Hg, Pb and Cd) and their potential health risks in commercial dried layer (Porphyra sm), we collected 45 samples from the major production areas on the western and southern coasts of Korea (Hwaseong, Seo-cheon, Gunsan, Muan, Shinan, undo, I-laenam, Wando, Jangheung, Goheung and Busan). The Hg, Pb and Cd concentrations were measured using inductively coupled plasma spectrometry (ICP-MS) or a mercury analyzer, The average Hg, Pb and Cd concentrations in the dried layer were 0.006±0, 0.017, 0.196+0.0614 and 0.894+0.4882 mg/kg, respectively, Based on the 2007 Korean Public Nutrition Report, these levels are 0.02, 0.11 and 2.47% of the provisional tolerable weekly intake (PTWI) for Hg, Pb and Cd, respectively, established by the FAO/WHO. The hazard quotient (HQ) determined from the ratio of exposure and safe levels were less than 1.0. Therefore, the levels of overall exposure to Hg, Pb and Cd for dried layer were below the recommended JECFA (Joint FAO/WHO Expert Committee on Food Additives) levels, which indicate safe levels for public health.
서식지에 따른 다소비 어류의 총수은 오염 및 위해 평가
조미라 ( Mira Jo ),김기현 ( Kihyun Kim ),조문래 ( Munrae Jo ),권지영 ( Jiyoung Kwon ),손광태 ( Kwangtae Son ),이희정 ( Heejung Lee ),김지회 ( Jihoe Kim ),이태식 ( Taeseek Lee ),강상인 ( Sangin Kang ),김진수 ( Jinsoo Kim ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.5
This study investigated the concentration of total mercury in 17 species of commonly consumed fishes and assessed the risk to human health from provisional tolerable weekly intakes PTWI% as affected by migratory characterization, such as migration and vagility. The measured mean concentrations of total mercury in these 17 species of commonly consumed fishes suggest that mean concentrations of total mercury in 10 species of migratory fishes (largehead hairtail, chub mackerel, Pacific saury, skipjack tuna, Pacific cod, anchovy, Alaska pollack, brown croaker, Japanese Spanish mackerel, yellow croaker and Pacific herring) were low compared to those in 7 species of demersal fishes (red stingray, brown sole, bastard halibut, conger eel, blackmouth angler, rockfish and filefish). Based on the mean concentrations, the PTWI% of total mercury among commonly consumed migratory fishes was 3.393%, which was higher than that of commonly consumed sedentary fishes (2.710%).
양식 넙치(Paralichthys olivaceus)에 경구투여한 Ampicillin 및 Amoxicillin의 어류체내 잔류량의 변화
손광태 ( Kwang Tae Son ),조미라 ( Mi Ra Jo ),오은경 ( Eun Gyoung Oh ),목종수 ( Jong Soo Mok ),권지영 ( Ji Young Kwon ),이태식 ( Tae Seek Lee ),송기철 ( Ki Cheol Song ),김풍호 ( Poong Ho Kim ),이희정 ( Hee Jung Lee ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.5
Residues of ampicillin and amoxicillin in the muscles of olive flounder Paralichthys olivaceus were investigated after oral doses of 20 mg/kg body weight/day of ampicillin and 40 mg/kg body weight/day of amoxicillin in a first examination and 40 mg/kg body weight/day of ampicillin and 80 mg/kg body weight/day of amoxicillin in a second examination for 5-6 days. The ranges of seawater temperatures in the 1st and 2nd examinations were 13.4-15.6oC and 16.8-21.4oC, respectively. Ampicillin and amoxicillin concentrations were analyzed by high performance liquid chromatography (HPLC). The average recovery rates of ampicillin and amoxicillin in fish samples ranged from 84.8- 95.2% and 100.8-103.8%, respectively. Residual concentrations in the olive flounder peaked on the 5th day, with average concentrations of 0.075 mg/kg and 0.311 mg/kg in the 1st examination, and 0.098 mg/kg and 0.630 mg/kg in the 2nd examination for ampicillin and amoxicillin, respectively, with maximum concentrations in muscle. In the 1st examination, ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentrations after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. In the 2nd examination, the ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentration after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. We suggest that the recommended withdrawal periods should be 3 days for ampicillin and 10 days for amoxicillin in the olive flounder.
Total Mercury Contents of Antarctic Toothfish Dissostichus mawsoni Caught in the Antarctic Sea
손광태,권지영,조미라,윤민철,송기철,최우석,연인자,김지호,이태식 한국수산과학회 2014 Fisheries and Aquatic Sciences Vol.17 No.4
Mercury is an element of special concern for human health. Measurements of total mercury levels in fish have been taken into consideration to assess risk. In this study, the Antarctic toothfish Dissostichus mawsoni was evaluated as a potential safe food source through measurement of total mercury contents. Total mercury concentrations in Antarctic toothfish ranged from 0.101 ± 0.047 mg/kg to 0.139 ± 0.075 mg/kg. The total mercury concentration was significantly correlated with macroscopic values including total fish length, weight, gonadosomatic index, and maturity (P < 0.01 or 0.05). Furthermore, according to the risk assessment, the total mercury body exposure rate from Antarctic toothfish ranged from 2.125% to 2.847% of the Provisional Tolerable Weekly Intake. Therefore, the Antarctic toothfish could be used as a potential safe seafood source.
어류 중 4계열 잔류 항생물질 검출을 위한 Lateral Flow Immunoassay Kit 개발
조미라 ( Mi Ra Jo ),손광태 ( Kwang Tae Son ),권지영 ( Ji Young Kwon ),목종수 ( Jong Soo Mok ),박홍제 ( Hong Jae Park ),김현용 ( Hyun Yong Kim ),김경동 ( Gyung Dong Kim ),김지회 ( Ji Hoe Kim ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.2
A lateral flow immunoassay kit based on antigen-antibody interactions was developed to detect residues of beta-lactams, quinolones, tetracyclines, and sulfonamides in farmed fish. Group-specific antibodies showing cross-reactivity with other antibiotics in the same group were produced in rabbits. The rabbits were immunized eight times to obtain the maximum titers. Antibodies were extracted from the antisera collected from the immunized rabbits and produced group-specific reactions with antibiotics from the four groups. A kit was prepared that optimize conditions for the antigen-antibody reaction, using colloidal gold conjugated antibodies, and was designed to detect the four groups of antibiotics simultaneously. The kit enabled the detection of antibiotics in the four groups at below maximum residue limits (MRLs), which were 200 μg/kg for tetracyclines, 100 μg/kg for sulfonamides, 50 μg/kg for beta-lactams, and 100 μg/kg for quinolones. The cross-reactivity of the antibodies ranged from 10-80% for the sulfonamides, 20- 100% for tetracyclines, 38-100% for quinolones, and 20-100% for the beta-lactams, confirming that the antibodies were group specific. The test kit was used 30 times to examine spiked antibiotics at the limits of detection (LODs) and all produced positive results, indicating high sensitivity. The LODs for the assay ranged from 4-20 ng/mL for beta-lactams, 25-50 ng/mL for sulfonamides, 20-100 ng/mL for tetracyclines, and 30-80 ng/mL for quinolones, and there were no false negative reactions at above these LODs. In addition, all of the LODs of the developed kit were correlated with high-performance liquid chromatography (HPLC) data. Our lateral flow immunoassay kit can simultaneously detect antibiotic residues from a large number of fish samples rapidly, strengthening the safety of domestic farmed and imported fish.
정충일,김광태,조남영,이길,오현석,정민정 한국축산식품학회 2002 한국축산식품학회지 Vol.22 No.3
This study was carried out to investigate the keeping quality of market milks collected from several dairy processing companies. Microbiological, chemical, and sensory properties of Ultra-high temperature(UHT) treated milks stored at 5, 10, 15℃ were evaluated during the storage of 15days. No significant difference during the storage was shown in bacterial counts, pH and titratable acidity. Standard plate count(SPC) was remained less than 20,000 cfu/ml after 15 day storage at 5, 10, 15℃. Also, Psychrotrophic bacterial count and Coliform count were not detected or detected at low level. The pH ranged from 6.55 to 6.83 and the titratable acidity ranged from 0.13% to 0.16%. In sensory evaluation, UHT milks showed normal flavor until 10days, but thereafter off-flavor was slightly developed. Consequently, UHT milks remained in good quality when stored at 5℃,10℃ until 15days except sensory properties, According to this experiment, the shelf-life of UHT milk can be extended to 10 days, even though maintaining the storage temperature strictly below 10℃ in milk market is not easy.
크롬친화세포종을 동반한 임신환자의 제왕절개 및 종양적출술의 마취관리 증례 보고
이정진,오용석,조광태 대한마취과학회 1993 Korean Journal of Anesthesiology Vol.26 No.3
The anesthetic management of a patient with pheochromoctoma in pregnancy is presented. Preoperative phenoxybenzamine therapy and careful intraoperative monitoring preduced successful cesarean section and tumor reseetion in this patient. Problems that occurred during mamagement of this patient are hypoxemia(R/0 pulmonary edema) and cardiovascular flucctuation according to the operative procedure. Hypoxemia oceurred possibly due to large amount of crystalloid infusion in a short time and was solved with applying positive end expiratory pressure and using diuretics. Blood pressure was controlled effectively with sodium nitroprusside or phenylephrine infusion according to the operative procedure. Preoperative pregnancy induced liver disease in this patient was recovered after delivery.
LC-MS/MS를 이용한 넙치(Paralichthys olivaceus)시료의 톨트라주릴 및 톨트라주릴 설폰 분석
홍도희,김아현,이가정,윤민철,손광태,김명석,김나영,정승희,조미라 한국수산과학회 2019 한국수산과학회지 Vol.52 No.5
Several studies investigating the prevention and treatment of external parasites in farmed olive flounder Paralichthys olivaceus have found that the anticoccidial agent toltrazuril sulfone is an effective antiparasitic. Prior to undertaking a full-scale study, we developed analytical methods to detect the levels of toltrazuril and toltrazuril sulfone in farmed flounder samples using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS). This analysis showed that LC-MS/MS changed the mobile phase and collision energy of toltrazuril and toltrazuril sulfone. This was validated using established conditions. Sample pre-treatment for this process involved extraction with dichloromethane and purification by liquid–liquid extraction in formic acid, acetonitrile, and h-hexane, followed by determination of all compounds by LC-MS/MS. Separation was achieved within 10 min by gradient elution using a Capcell Pak C18 (3.0 μm, 100×2.0 mm) analytical column (Shiseido UG 120V) with a mixture of 0.1% (v/v) formic acid and acetonitrile. Multiple reaction monitoring was used for selective detection of toltrazuril and toltrazuril sulfone. This method yields satisfactory results for linearity, precision, and limits of quantification. Therefore, the method established in our study will serve as a basis for further research on parasite control by toltrazuril and toltrazuril sulfone.