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정형섭,이성규,권희석,현재경 한국구조생물학회 2017 Biodesign Vol.5 No.3
Cryo-electron microscopy (cryo-EM) single particle analysis has become a mainstream structural biology tool andtherefore access to high-end transmission electron microscope (TEM) dedicated to high resolution cryo-EM is critical. At Korea Basic Science Institute (KBSI), a facility dedicated to biological electron microscopy was established in 2016,and High Resolution Bio-TEM (HR Bio-TEM, Titan Krios, Thermo Fisher Scientific Inc., USA) is now accessible to nationaland international researchers. This report describes major features of the HR Bio-TEM and the facility, along with overallworkflow of cryo-EM image data acquisition.
정형섭,박상은,김진수,박노욱,홍상훈 대한원격탐사학회 2019 大韓遠隔探査學會誌 Vol.35 No.6
As there is a growing interest in remote sensing applications, the Korean Society of Remote Sensing has published several special issues in which articles on specific research topics were collected. In this special issue, we first review the research topics on several special issues published in the Korean Journal of Remote Sensing by 1) the National Institute of Agricultural Sciences (NAS), the Korea Environment Institute (KEI), the Ministry of Environment (ME) and the Korea Aerospace Research Institute (KARI) in 2017 and 2) the Korea Institute of Ocean Science and Technology (KIOST), the Korea Polar Research Institute (KOPRI), KARI, and Korea Meteorological Administration (KMA) in 2018. Then, research articles on the remote sensing applications in Korea are introduced. 최근 원격탐사 활용이 더욱 늘어남에 따라 연구주제를 모아 게재하는 특별호가 진행되었다. 이번 특별호에서는 2017년 국립농업과학원, 한국환경정책평가연구원, 환경부 및 한국항공우주연구원에서 진행한 연구주제와 2018년 한국해양과학기술원, 극지연구소, 한국항공우주연구원, 기상청에서 진행한 연구주제를 검토하고, 한국에서의 원격탐사 활용에 관한 연구들을 소개하고자 한다.
정형섭,우재성,권희석,류범한 한국구조생물학회 2019 Biodesign Vol.7 No.2
The Falcon 2 camera (Thermo Fisher Scientific Inc., USA) mounted in High Resolution Bio-TEM at Korea Basic Science Institute (KBSI) was upgraded to Falcon 3EC in August 2017 (Jeong et al., 2017). Compared with Falcon 2, Falcon 3EC has better sensitivity and higher frame rate and can be operated in electron counting mode. These changes significantly increase the data quality, thereby increasing the resolution of the 3D structure. In the benchmarking test of this machine, the structure of inhibitor-free beta-galactosidase has been solved at 2.16 Å. Here we introduce main features of the new detector, and discuss about major considerations required for its operation and data collection strategies to achieve the near atomic resolution.
정형섭,홍상훈,원중선 대한원격탐사학회 2008 大韓遠隔探査學會誌 Vol.24 No.5
Qualified ground control points (GCPs) are required to construct a digital elevation model(DEM) from a pushbroom stereo pair. An inverse geolocation algorithm for extracting GCPs from ERSSAR data and the SRTM DEM was recently developed. However, not all GCPs established by this methodare accurate enough for direct application to the geometric correction of pushbroom images such as SPOT,IRS, etc, and thus a method for selecting and removing inaccurate points from the sets of GCPs is needed.In this study, we propose a method for evaluating GCP accuracy and winnowing sets of GCPs throughorientation modeling of pushbroom image and validate performance of this method using SPOT stereo pairof Daejon City. It has been found that the statistical distribution of GCP positional errors is approximatelyGaussian without bias, and that the residual errors estimated by orientation modeling have a linearrelationship with the positional errors. Inaccurate GCPs have large positional errors and can be iterativelyeliminated by thresholding the residual errors. Forty-one GCPs were initially extracted for the test, withmean the positional error values of 25.6 m, 2.5 m and -6.1 m in the X-, Y- and Z-directions, respectively,and standard deviations of 62.4 m, 37.6 m and 15.0 m. Twenty-one GCPs were eliminated by theproposed method, resulting in the standard deviations of the positional errors of the 20 final GCPs beingreduced to 13.9 m, 8.5 m and 7.5 m in the X-, Y- and Z-directions, respectively. Orientation modeling ofthe SPOT stereo pair was performed using the 20 GCPs, and the model was checked against 15 map-based points. The root mean square errors (RMSEs) of the model were 10.4 m, 7.1 m and 12.1 m in X-,Y- and Z-directions, respectively. A SPOT DEM with a 20 m ground resolution was successfully constructedusing a automatic matching procedure.