복합미생물을 이용한 수산폐기물의 분해특성

정해윤,정해윤,김중균 한국생명과학회 2001 생명과학회지 Vol.11 No.1

Fifteen species of microorganisms were isolate form the intestines of fishes, fish feed, and ferment. Eleven microorganisms except HY4, HY8, HY12, and HY13 were Gram-positive, and HY1, HY2, HY3, HY5, HY6, and HY7 produced lactic acid. The species of HY1, HY2, HY3, HY4, HY5, HY6, HY13, and HY14 showed some growth in the medium containing 1% of NaCl. Except HY6, HY7, HY8, HY12 and HY5, 10 isolates had proteolytic activity, whereas only HY13 and HY14 had lipase activity. From all the results four isolates (HY3, HY4, HY13 and HY14) were chosen for the degradation of fish wastes. There was no mutual inhibition among the microorganisms, and the optimum temperature and pH for the growth of the mixed culture were found to be 3 2$\^{C}$ and 7, respectively. Under the optimum growth conditions the maximum optical density and the maximum specific growth rate were estimated to be 2.35 and {TEX}$0.46h^{-1}${/TEX}, respectively. Major microorganisms in the mixed culture at the log-phase were HY3 and HY4, which occupied 70%. The degrading efficiency of fish waste by the mixed microorganisms was 2.3 times higher, compared to control. The total amount of free amino acids in the degraded products from fish wastes was 39g/100g protein and little odor was produced by the mixed microorganisms after 48 hours.

한글판 건강 증진 생활양식 프로파일(Korean Version of Health Enhancement Lifestyle Profile; K-HELP)의 개발 및 신뢰도 연구

정해윤,정민예,유은영,강대혁 대한작업치료학회 2015 대한작업치료학회지 Vol.23 No.2

목적 : 본 연구는 노인의 건강 증진 생활양식을 파악하고자 한글판 건강 증진 생활양식 프로파일(Korean Version of Health Enhancement Lifestyle Profile; K-HELP)을 개발하고 K-HELP의 신뢰도를 검 증하고자 하였다. 연구방법 : 작업치료사 Hwang에 의해 미국에서 개발된 Health Enhancement Lifestyle Profile (HELP)의 한글판 개발을 위해 번안과정 및 내용타당도 검증을 거치고 국내 상황에 맞게 문항을 수정하여 K-HELP를 완성하였다. 완성된 K-HELP의 신뢰도 검증을 위해 내적일치도 및 검사-재검사 신뢰도 검증을 실시하였다. 결과 : 완성된 한글 번역본 HELP을 보건의료전문가 9인에게 회부하여 검증받은 56문항에 대한 내용타당 도지수(Content Validity Index; CVI)는 .97로 높게 나타났다. 정상 노인 483명을 대상으로 한 신뢰도 검증 결과 전체 문항에 대한 내적일치도는 .82로 높은 수준으로 나타났고, 검사-재검사 신뢰도는 .99로 매우 높은 상관관계를 보였다(p<.01). 결론 : 본 연구는 K-HELP의 개발과 신뢰도 검증을 통해 K-HELP가 노인의 생활양식과 건강수준을 측 정하는데 신뢰도가 입증된 도구임을 증명하였다. 본 연구를 토대로 K-HELP가 노인 건강 증진에 대한 질병 예방 및 적절한 중재를 제공하기 위해 사용되는 유용한 평가도구로 활용될 수 있을 것이다. Objective : The purposes of this study were to develop a Korean version of the Health Enhancement Lifestyle Profile (K-HELP), to evaluate the overall lifestyles and health behaviors of the elderly, and to verify the reliability of K-HELP. Methods : The original Health Enhancement Lifestyle Profile (HELP) was developed in the U.S.A. by Jengliang Eric Hwang, who is an occupational therapist. To develop K-HELP, a series of steps were taken; First, translation and reverse-translation processes were conducted by an expert committee. To establish the content validity, a modification process was taken for adaptation to the Korean circumstances by health professionals. Second, for a validation of the reliability, the internal consistency and test-retest reliability of the content were measured. Results : The value of the Content Validity Index (CVI) was .97. The CVI was derived from an assessment by nine health care experts, which aimed to determine the suitability of 56 questions. 483 healthy elderly people participated in this reliability study. The value of internal consistency (Cronbach’s α) was .82. In addition, there was a very high and statistically significant test-retest reliability (r=.99)(p<.01). Conclusion : This study proved that K-HELP is a reliable tool to evaluate a senior's lifestyle and health status through the development of K-HELP and a verification of its reliability. Based on this study, K-HELP can be used as a useful assessment tool in the health related field of the elderly by increasing the understanding of the lifestyles, health behaviors, and health levels of the elderly in preventing diseases and providing appropriate rehabilitation programs.

국회회의록 형태 주석 말뭉치의 구축 과정과 형태 사용 빈도 분석

정해윤,김지영,김교연 연세대학교 언어정보연구원 2023 언어사실과 관점 Vol.58 No.-

이 연구는 국회회의록 형태 주석 말뭉치의 구축 과정을 소개하고 구축된 말뭉치의 형태소 사용 빈도를 밝히는 것을 목적으로 한다. 이 연구에서 구축한 국회회의록 형태 주석 말뭉치는 1940년대부터 2010년대까지 총 189회차 회의록으로 이루어진 말뭉치 언어 자료로서 총 1,037,435 어절로 구성되었다. 품사 범주별로 고빈도 형태소 상위 40위를 살피고 서상규(2015) 한국어 구어 빈도 사전1(잦기순) 과의 비교를 통해 국회회의록 말뭉치 또한 구어 자료로서 일반적인 구어 자료와 유사한 성격을 지니기는 하지만 ‘국회 회의’라는 특수 맥락과 공적 대화의 특성이 더 부각된 자료라는 점에서 몇몇 차이가 있음을 확인하였다.

현행 「한글 맞춤법」 준말 규정에 대한 일고

정해윤 한말연구학회 2022 한말연구 Vol.63 No.15

This paper aims to review the abbreviation rules in the current “Hangeul Matchumbeop”(한글맞춤법, 1988) and to highlight the problems associated with these rules and to also propose a road-map to correct and improve them. To this end, Chapter 2 examines the evolutionary process up to the current “Hangeul Matchumbeop(한글맞춤법, 1988)” rules. The abbreviation notation first appeared as a separate code from the “Hangeul Matchumbeop Tongiran”(한글마춤법 통일안, 1933) and later, the revision of the rules was discussed in the “Hangeul Matchumbeop revision draft”(한글맞춤법 개정 시안, 1978) conducted by the Ministry of Education which led to the current regulations. In Chapter 3, each regulation was reviewed to examine the aptness of the abbreviation rules of the current “Hangeul Matchumbeop”(한글맞춤법, 1988). In particular, 2,526 abbreviated head-words listed as head-words in the “Pyojun-Gugeo-Daesajeon”(표준국어대사전) under the current code were compared. Thereafter the appropriateness of the rules was reviewed in a direction in which all the abbreviation-related head-words could be explained, and the direction of revision was briefly considered. In Chapter 4, we synthesized the current body of data and presented the revised abbreviation rules proposed by this study. The most important thing to consider in the revision process is practicality and conciseness. Therefore, while there are 9 abbreviations in the “Hangeul Matchumbeop”(한글맞춤법, 1988), in this study, 2 more were added and a total of 11 abbreviations were proposed. In addition to the two newly added provisions, the existing rules were also revised in terms of detail by changing the attached provisions or changing the examples. Finally, Chapter 5 summarizes this study. This study is meaningful in that it reveals the problems of the abbreviation rules, which have not been treated as important in studies related to Korean spelling, and in that it suggests the direction for further revision. .

The Blocking of c-Met Signaling Induces Apoptosis through the Increase of p53 Protein in Lung Cancer

정해윤,주현정,박종국,김열홍 대한암학회 2012 Cancer Research and Treatment Vol.44 No.4

Purpose c-Met is an attractive potential target for novel therapeutic inhibition of human cancer, and c-Met tyrosine kinase inhibitors (TKIs) are effective growth inhibitors of various malignancies. However, their mechanisms in anticancer effects are not clear. In the present study,we investigated the possibility that blocking c-Met signaling induces p53-mediated growth inhibition in lung cancer. Materials and Methods The growth inhibitory effects of c-Met TKI (SU11274) on lung cancer cells and a xenograft model were assessed using the MTT assay, flow cytometry, and terminal deoxyribonucleotide transferase-mediated nick-end labeling staining. The role of p53 protein in the sensitivity of c-Met TKI (SU11274) was examined by Western blot analysis and immunohistochemistry. Results SU11274 significantly induced apoptosis in A549 cells with wild-type p53, compared with that in Calu-1 cells with null-type p53. SU11274 increased p53 protein by enhancing the stability of p53 protein. Increased p53 protein by SU11274 induced up-regulation of Bax and PUMA expression and down-regulation of Bcl-2 expression, subsequently activating caspase 3. In p53 knock-out and knock-in systems, we confirmed that SU11274 caused apoptosis through the p53-mediated apoptotic pathway. Likewise, in the A549 xenograft model, SU11274 effectively shrank tumor volume and induced apoptosis via increased p53protein expression. Blocking c-Met signaling increased the level of p53 protein. Conclusion Our finding suggested that p53 plays an important role in SU11274-induced apoptosis, and p53 status seems to be related to the sensitivity to SU11274 in lung cancer.

수산폐기물의 발효사료화를 위한 발효미생물의 대량생산

정해윤,김중균 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.27 No.-

학회순례 - 47년 창립, 정회원만 전국에 1천6백88명 "대한안과학회"

정해윤,Jeong, Hae-Yun 한국과학기술단체총연합회 2000 과학과 기술 Vol.33 No.3

1947년 20여명의 발기로 창립된 대한안과학회는 반세기가 넘는동안 매년 봄ㆍ가을에 학술대회를 열고 학회지도 월간으로 발행하는 등 1천6백88명의 회원들이 뭉쳐 왕성한 학술활동을 펼치고 있다. 학회업무는 현재 이사장 중심제로 총무, 편집, 재무 등 9명의 상임이사들이 제반업무를 소관사항에 따라 처리하고 있다.

국회 회의록 말뭉치 구축에 관하여 - 국회 본회의록을 중심으로

정해윤,김지영,김교연 한국사전학회 2022 한국사전학회 학술대회 발표논문집 Vol.2022 No.2

직장풍선을 삽입한 전립선암 환자의 수술 후 방사선 치료 시 용적변조와 세기변조방사선치료계획 비교 평가

정해윤,석진용,홍주완,장남준,최병돈,박진홍,Jung, hae youn,Seok, jin yong,Hong, joo wan,Chang, nam jun,Choi, byeong don,Park, jin hong 대한방사선치료학회 2015 대한방사선치료학회지 Vol.27 No.1

목 적 : 전립선암의 방사선치료는 치료기법에 따라 주요장기와 정상조직의 선량분포에 차이가 나타난다. 본 연구에서는 전립선암 환자의 수술 후 방사선치료 시 직장풍선을 삽입한 환자에게 회전수를 달리한 용적변조회전치료(volumetric modulated arc therapy, VMAT)계획과 세기변조방사선치료(intensity modulated radiation therapy, IMRT)계획을 각각 수립하여 선량분포 특성을 비교하고 치료의 효율성을 평가하고자 한다. 대상 및 방법 : 본원에서 전립선암 수술 후 방사선 치료를 시행한 10명의 환자를 대상으로 하였다. 직장풍선을 삽입한 환자의 CT영상을 3 mm 두께로 획득하고 Eclipse (Ver 11.0, Varian, Palo Alto, USA)를 사용하여 HD120MLC가 장착된 Truebeam STx (Varian, Palo Alto, USA)의 10 MV 에너지를 적용하였다. 환자마다 1 Arc, 2 Arc VMAT계획과 7조사면의 IMRT계획을 수립하였으며 각 치료계획의 선량체적제한과 plan normalization 값은 동일하게 적용하였다. 수립된 세 가지 치료계획을 평가하기위해 PTV의 coverage, conformity index (CI), homogeneity index (HI)를 비교하였고, PTV주변 정상조직의 선량퍼짐정도를 알아보기 위해 50% 등선량체적과 PTV체적의 비($R_{50%}$)를 산출하였다. 결정장기 (organ at risk, OAR)에서는 직장의 $D_{25%}$와 방광의 $D_{mean}$을 비교하였고 치료의 효율성을 평가하기 위해서 총 MU와 조사시간을 측정하였으며, 각 평가항목별 결과는 환자 10명의 평균값으로 비교분석하였다. 추가적으로 선량전달 정확도를 검증하기 위해 EPID를 이용한 portal dosimetry를 진행하였다. 결 과 : 각 치료계획에서 나타난 PTV coverage와 HI의 차이는 크지 않았지만 CI는 1A-VMAT, 2A-VMAT, 7F-IMRT에서 각각 1.036, 1.035, 1.230으로 VMAT에 비해 7F-IMRT가 높았고(p=0.00), $R_{50%}$는 3.083, 3.054, 3.991로 2A-VMAT이 제일 낮았고 7F-IMRT에서 가장 높게 나타났다(p=0.00). 치료계획에 따른 직장의 $D_{25%}$는 VMAT에서 비슷했지만 7F-IMRT가 약 7% 높게 나타났고(p=0.02), 방광의 $D_{mean}$은 큰 차이가 없었다(P>0.05). 총 MU는 1A-VMAT, 2A-VMAT, 7F-IMRT에서 각각 494.7, 479.7, 757.9로 7F-IMRT가 가장 많았고(p=0.00), 조사시간은 65.2초, 133.1초, 145.5초로 1A-VMAT이 확연히 짧았다(p=0.00). portal dosimetry 검증에서는 모든 치료계획에서 99.5% 이상의 gamma pass rate(2 mm, 2%)을 보였다(P=0.00). 결 론 : 본 연구결과 직장풍선을 삽입한 전립선암 환자의 수술 후 방사선치료 시, 치료기법에 따른 PTV coverage의 차이는 크지 않았지만 1A, 2A-VMAT이 7F-IMRT에 비해 정상조직과 직장선량을 낮추는데 효과적이었다. VMAT중에서는 $R_{50%}$와 MU가 2A-VMAT에서 다소 낮았지만 조사시간이 짧은 1A-VMAT이 치료에 더 효율적이며 환자의 치료 중 움직임 또한 줄일 수 있을 것으로 사료된다. Purpose : The dose distribution of organ at risk (OAR) and normal tissue is affected by treatment technique in postoperative radiation therapy for prostate cancer. The aim of this study was to compare dose distribution characteristic and to evaluate treatment efficiency by devising VMAT plans according to applying differed number of arc and IMRT plan for postoperative patient of prostate cancer radiation therapy using a rectal balloon. Materials and Methods : Ten patients who received postoperative prostate radiation therapy in our hospital were compared. CT images of patients who inserted rectal balloon were acquired with 3 mm thickness and 10 MV energy of HD120MLC equipped Truebeam STx (Varian, Palo Alto, USA) was applied by using Eclipse (Version 11.0, Varian, Palo Alto, USA). 1 Arc, 2 Arc VMAT plans and 7-field IMRT plan were devised for each patient and same values were applied for dose volume constraint and plan normalization. To evaluate these plans, PTV coverage, conformity index (CI) and homogeneity index (HI) were compared and $R_{50%}$ was calculated to assess low dose spillage as per treatment plan. $D_{25%}$ of rectum and bladder Dmean were compared on OAR. And to evaluate the treatment efficiency, total monitor units(MU) and delivery time were considered. Each assessed result was analyzed by average value of 10 patients. Additionally, portal dosimetry was carried out for accuracy verification of beam delivery. Results : There was no significant difference on PTV coverage and HI among 3 plans. Especially CI and $R_{50%}$ on 7F-IMRT were the highest as 1.230, 3.991 respectively(p=0.00). Rectum $D_{25%}$ was similar between 1A-VMAT and 2A-VMAT. But approximately 7% higher value was observed on 7F-IMRT compare to the others(p=0.02) and bladder Dmean were similar among the all plan(P>0.05). Total MU were 494.7, 479.7, 757.9 respectively(P=0.00) for 1A-VMAT, 2A-VMAT, 7F-IMRT and at the most on 7F-IMRT. The delivery time were 65.2sec, 133.1sec, 145.5sec respectively(p=0.00). The obvious shortest time was observed on 1A-VMAT. All plans indicated over 99.5%(p=0.00) of gamma pass rate (2 mm, 2%) in portal dosimetry quality assurance. Conclusion : As a result of study, postoperative prostate cancer radiation therapy for patient using a rectal balloon, there was no significant difference of PTV coverage but 1A-VMAT and 2A-VMAT were more efficient for dose reduction of normal tissue and OARs. Between VMAT plans. $R_{50%}$ and MU were little lower in 2A-VMAT but 1A-VMAT has the shortest delivery time. So it is regarded to be an effective plan and it can reduce intra-fractional motion of patient also.

Correlation between Retinoic Acid Sensitivity and IGFBP-3, AFP Protein Expression in Hepatoma Cell Lines

정해윤,Yong Myung Lee,Young Do Yoo,Jun Suk Kim,Sun-Hee Park,Yeul Hong Kim 대한암학회 2003 Cancer Research and Treatment Vol.35 No.1

Purpose: Retinoic acid (RA) has been known to inhibit the proliferation, and to induce apoptosis, of various cancer cell lines. We investigated the correlation between the protein levels of the RAR and RXR receptor families, IGFBP-3 and AFP, and the RA sensitivity in hepatoma cell lines. Materials and Methods: The cell growth inhibition was examined by assaying various 1 to 10μM RA treated hepatoma cell lines. Western blot analysis for the RAR and RXR families, AFP and IGFBP-3 were performed after treatment with 10μM RA. Results: The 1 to 10μM RA treatment induced growth inhibition in the SNU368, SNU354, SNU398 and HepG2 cells. The cell growth of SNU449 and Hep3B were not suppressed by 1μM, but were slightly suppressed by 10 μM RA. An increased expression of IGFBP-3 in HepG2, SNU354, SNU398 and SNU368 cells, and a decreased expression of α-fetoprotein (AFP), was observed from the western blot analysis in all hepatoma cells tested, whereas no confirmed tendency of RAR expressions was seen. Conclusion: Our result showed that the growth inhibition of RA differed according to the sensitivity of the type of cells to RA. We supposed that RA-induced cell growth inhibition may be related to the expressions of IGFBP-3 and AFP, but no exact correlation exists between the growth inhibition and receptor expression status in hepatoma cell lines. (Cancer Rese Treat. 2003;35:59-65)