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AMC-HN-4 두경부 암세포에서 Photofrin을 이용한 광역학 치료의 세포독성 연구
정필상,신장인,황희준,장소영,강정욱,권필승,이정구,안진철 대한광역학학회 2007 대한광역학학회지 = Journal of Korean Photodynamic Associat Vol.4 No.2
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common malignancy worldwide. The molecular mechanisms involved in the development and progression of these carcinomas are not well known. The aim of this study was to investigate the mechanism of cell death by photodynamic therapy in head and neck cancer cell line. AMC-HN-4 cells were subjected to photodynamic therapy with photofrin (0.1-50 ug/ml) for different incubation time before photoirradiation. Photodynamic toxicity was investigated 24h after treatment. The type of cell death was determined using Hoechst 33342 stain methods. In this study, there was cytotoxicity of photofrin-induced photodynamic therapy in the AMC-HN-4 cells, and it was dose-dependent manner. Cell death by photofrin-induced photodynamic therapy was caused predominantly by apoptosis. Photodynamic therapy with photofrin significantly enhanced photocytotoxicity and apoptosis.
Superiorly based flap을 이용한 기관절개술
정필상,이정구,정필섭,김영훈 대한기관식도과학회 1995 大韓氣管食道科學會誌 Vol.1 No.1
The superiorly based flap tracheostomy(SBFT) has been advocated as an new technique of tracheostomy to manage a wide variety of causes of upper airway obstruction. This technique has particular applicability in patients who require long term tracheostomy such as in bilateral vocal cord paralysis and severe obstructive sleep apnea. SBFT has numerous advantages such as shortening of the gap between the skin and trachea : construction of a self-sustaining tract ; circumferential mucocutaneous junction to reduce infection, granulation tissue, bleeding, and stenosis of the tract : avoidance of the laryngotracheal damage : easy placement of a tracheostomal stent to promote speech, coughing and swallowing. Most of all, this technique can reduces the suprastomal buckling by the support of the superiorly based tracheal flap, and thus prevents the stenosis of suprastomal airway. The disadvantage of SBFT is more time-consuming procedure than the conventional tracheostomy, A retrospective analysis of 8 patients undergoing SBFT between June, 1994 and March, 1995 in Dankook University Hospital was performed to present the surgical technique and com-plication rates. The average duration of follow up was 11 months. The complications were consisted of a wound infection and a sternal granulation. The other complications including wound dehiscence, tracheitis, pneumonia, tracheal granulation, sternal narrowing and subglottic stenosis were not experienced.
정필상,김진왕,이정옥,이정구,오충훈,김유찬,정상운 대한성형외과학회 2005 Archives of Plastic Surgery Vol.32 No.2
Low-power laser(LPL) delivers a small amount of energy without elevation of tissue temperature. LPL has been reported to have biostimulation effects including anti-inflammatory, analgesic, regenerative, immunocorrective, and vasodilative effects. However, the effect of LPL on hair growth has rarely been studied. We investigated the effect of LPL on hair growth in the mouse. After depilation of back skin of mice, we classified the mice into 4 groups: control, laser irradiated group, MoandMore applied group, and Spella 707 applied group. Laser irradiation or application of these drugs were performed on the back skin of the mice for 30 days. The results are summarized as follows. Hair growth of control was first observed at 13 days after depilation, and complete hair regrowth was observed at 25 days. Hair growth of both laser irradiation group and MoandMore applied group was first observed at 9 days after depilation, and complete hair regrowth was observed at 20 days. Hair growth of Spella 707 applied group was firstly observed at the 9 days after depilation, and complete hair regrowth was observed at the 15 days. Hair growth started at the irradiation site in the laser irradiation group, but it started at the random sites in other groups. In conclusion LPL irradiation have a stimulating effect on the hair growth in the mouse.
정필상,정신근,이진구,정용원 대한이비인후과학회 2003 대한이비인후과학회지 두경부외과학 Vol.46 No.12
Background and Objectives:Leukoplakia is a descriptive term designating a white patch or plaque of the mucosa and must be complemented by histology. The aim of this study was to analyze the laryngeal leukoplakia clinically. Materials and Method: 48 patients were included from 1995 to 2001. All presented themselves with laryngeal leukoplakia in telescopic examination. The available medical record are age, sex, duration of smoking, histopathological finding, treatment and follow up result. Results:The patients were composed of 46 males and 2 females. There were 7 patients (15%) with specific diagnostic lesion (polyps, lymphoma, tuberculosis, chronic inflammation and papilloma), 19 patients (40%) with simple hyperplasia or hyperkeratosis, 4 patients (8%) with mild dysplasia, 2 patients (4%) with moderate dysplasia, 2 patients (4%) with severe dysplasia, 2 patients (4%) with carcinoma in situ, and 1 patient (2%) with microinvasive carcinoma and 11 patients with invasive squamous cell carcinoma. Rebiopsy was performed to 10 cases during follow up period. Conclusion:The results of our study show that the leukoplakia was presented in 15% of all cases with specific diagnostic lesion and that the patient was treated according to their specific diagnosis. Almost all of the case with precancerous conditions were cured with cord stripping, cordectomy or radiation therapy. (Korean J Otolaryngol 2003;46:1064-9)
두경부 편평상피암 세포주에서 9-Hydroxypheophorbide-a를이용한 광역학치료의 ROS 발현 양상과 세포사에 관한 연구
정필상,박으뜸,이기일,안진철 대한이비인후과학회 2006 대한이비인후과학회지 두경부외과학 Vol.49 No.12
Background and Objectives:During PDT, photosensitizer accumulates in the cell and irradiation forms ROS. ROS leads to activationof apoptoticpathway and cell death. Elevated intracellular calcium is known to play important role in apoptotic pathway.There are two type of ROS formation. The type of ROS formation differs in type of photosensitizers. We designed the experimentto define the relationship of ROS and cell death in PDT. Materials and Method:AMC-HN3 cells were cultured. Using aCaspACE assay kit, we measured caspases-3 activity after PDT. We also observed intra-cellular calcium concentrations usingconfocal microscopy (calcium green-1 stain) after PDT. To determine which type of reaction occursduring ROS formation, MTTassay was performed. Results:Confocal microscopy showed that ROS had formed at the site of photosensitizer formation afterPDT. After PDT, intracellular calcium increased. MTT assay showed more viability increase in blocking type II reaction. Caspaseassay showed highest level after 4hrs. Conclusion:ROS is formed at the site photosentizer formation after PDT. Type II reactionwas the main type of ROS formation. Apoptosis was main pathway of cell death in low dose of photosensitizer after PDT. (KoreanJ Otolaryngol 2006;49:1194-200)