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BCG 및 Acriflavine-Guanosine 복합제(AG60)가 Ehrlich 종양세포를 이식한 흰생쥐 위점막의 벽세포에 미치는 영향
고정식(Jeong-Sik Ko),정인광(Inn-Gwang Jeong),박경호(Kyung-Ho Park),안의태(E-Tay Ahn) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.6
이 연구는 고농도의 BCG를 피부밑조직에 반복 투여하였을 때와 새로이 개발되어 항암효과는 높으나 세포독성이 적다 는 acriflavine-guanosine복합제(AG60)를 반복 투여하였을 때 위점막 벽세포에 미치는 영향을 알기 위하여 시행하였다. 실험동물로는 체중 25 g 내외의 ICR흰생쥐를 사용하였으며 정상대조군을 제외한 종양대조군, BCG 및 AG60 투여군의 동물에는 샅부위 피부밑조직에 각각 1×107개의 Ehrlich종양세포를 이식하였다. BCG 투여군은 종양세포이식 다음날부터 농축 건조된 BCG (0.6×108~6.4×108 CFU, 27 mg/vial, Connaught Lab. Canada)를 10 ml의 생리식염수에 용해시킨 다음, 일정량 (0.5 ml/25 g B.W.: 0.03×108~0.32×108 CFU)을 하루건너 한 번씩 피부밑조직에 주사하였다. AG60 투여군은 30 mg/kg의 AG60 (Acriflavine : Guanosine = 1 : 1)을 하루건너 한번씩 피부밑조직에 주사하였다. 종양대조군은 종양세포이식 후에 0.2ml의 생리식염수를 피부밑조직에 주사하였고, 정상대조군은 종양세포를 이식하지 않은 동물을 사용하였으며 각 군에는 5마리씩의 동물을 배정하였다. 종양대조군, BCG 투여군 및 AG60 투여군은 각각 7회씩 생리식염수, BCG 또는 AG60을 투여한 다음날 ether 마취하에 앞배벽을 열어 위조직을 절취하였다. 절취한 조직은 통상적인 방법으로 전자현미경 관찰용 조직절편을 만든 후 전자현미경으로 위샘부위를 관찰하였다. 종양대조군, BCG 투여군 및 AG60 투여군의 경우, 대부분의 벽세포는 세포속모세관 속의 미세융모의 모습이 정상대조군 에 비하여 매우 불규칙하였으며 서로 밀착되어 있어서 속공간이 거의 구별되지 않았다. 또한 BCG 투여군과 AG60 투여군 의 경우, 종양대조군에 비하여 벽세포내에서 수초구조, 용해소체 및 뭇소포체가 많이 관찰되었다. 한편 BCG 투여군의 경우 세관소포를 이루는 막구조가 심하게 파괴되어 흐트러져 보이는 벽세포가 관찰되었으나 AG60 투여군에서는 이와 같은 현상을 거의 관찰 할 수 없었다. 이상의 결과로 보아 Ehrlich 종양세포를 이식한 동물에 BCG를 반복 투여하면 생쥐 위점막의 벽세포는 미세구조들이 손상을 받게되어 분비기능이 다소 억제되나, AG60은 벽세포의 분비기능에 큰 손상을 주지 않는 것으로 생각된다. This experiment was performed to evaluate the morphological responses of the gastric parietal cells of mouse inoculated with Ehrlich carcinoma cells, following administration of Bacillus Calmette-Guérin (BCG) or acriflavine-guanosine composition (AG60, Taerim Pharm. Co. Seoul, Korea). In the experimental groups, each mouse was inoculated with 1×107 Ehrlich carcinoma cells subcutaneously in the inguinal area. From next day, 0.2 ml of saline, BCG (0.03×108~0.32×108 CFU) or AG60 (30 mg/kg) was injected subcutaneously to the animals every other day. Animals were sacrificed on the 14th day from the first injection. Pieces of the tissue were taken from the stomach, prefixed with 2.5%glutaraldehyde-1.5% paraformaldehyde, followed by post-fixation with 1% osmium tetroxide. The ultrathin sections stained with uranyl acetate and lead citrate were observed with a JEM 100CX-II electron microscope. In the experimental control, the BCG and the AG60 treated groups, most parietal cells showed reduced lumenal spaces of the intracellular canaliculi, since microvilli of intracellular canaliculi were very irregularly shaped and crowed with each other. And in the BCG and the AG60 treated mice, myelin figures, lysosomes and multivesicular bodies in the parietal cells were observed more frequently than in those of the experimental control ones. In the BCG treated rats, membranes of the tubulovesicles of the parietal cells were disintegrated, but the similar changes were not observed in the AG60 treated mice,. Above results suggest that the BCG treated animals inoculated with Ehrlich carcinoma cells might suffer from reduced acid secretion of the parietal cell, since the disintegration of the tubulovesicular membranes in the parietal cells are occurred following injections. Whereas AG60 dose not affect remakably defect on the parietal cells.
조기진통의 예측지표로서 자궁경부 및 질내 태아파이브로넥틴의 임상적 유용성
김수평,이종건,나종구,김사진,김용욱,이종승,변태섭,정인광,나윤택 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.10
Prematurity is the major cause of neonatal mortaliy and morbidity. The prediction and early arrest of preterm uterine contractions is the mainstay of the management of the preterm labor. Several biophysical methods and biochemical markers are available, but not satisfactory for the prediction of preterm labor. The aim of this study is to determine the clinical efficacy of fetal fibronectin as a biochemical predictor of preterm labor. Fetal fibronectin was obtained at external os and posterior vaginal fornix in 118 women who have irregular uterine contractions with intact membranes and, in 113 women who complained of watery vaginal discharges without visible leakage. The cervicovaginal fetal fibronectin was measured quantitatively by fetal fibranectin immunoassay in 118 patients and qualitatively by fetal fibronectin membrane immunoassay in 113 patients. The results were considered positive when specimens had a fetal fibronectin concentration above 50 ng/ml in women who have irregular uterine contractions with intact membranes. The results obtained were as follows; 1. Fetal fibronectin concentrations in women with or without irregular uterine contractions with cervix dilatation($lt;=2cm) and intact membranes were 229.18+-68 ng/ml, 16.8+-9.2 ng/ml, respectively. 2. In the efficacy of fetal fibronectin for the prediction of preterm labor in women who have irregular uterine contractions and intact membrane, the sensitivity, specificity, positive predictive value and negative predictive value are 83.3%, 85.2%, 65.8% and 93.8%, respectively. There were significant correlation between the positivity of the fetal fibronectin and interval(diagnosis to delivery), gestational weeks at delivery and neonatal body weight. 3. In the efficacy of fetal fibronectin for the prediction of preterm labor in women who complained of watery vaginal discharges without visible leakage, the sensitivity, specificity, positive predictive value and negative predictive value are 62.5%, 93.3%, 71.4% and 90.2%, respectively. There were significant correlation between the positivity of the fetal fibronectin and interval(diagnosis to delivery), gestational weeks at delivery and neonatal body weight. 4. in the association between fetal fibronectin membrane immunoassay and nitrazine test in women who complained of watery vaginal discharges without visible leakage, the accuracy was 83.2%. This results reflect that positive cerviovaginal fetal fibronectin in women who have irregular uterine contractions or in women who complained of watery vaginal discharges maybe a useful biochemical predictor for the prediction of preterm labor.
인유두종 바이러스검출을 이용한 자궁경부 상피내종양의 분자생물학적 진단방법
남궁성은,박종섭,김용욱,허수영,박동춘,정인광,나윤택,김차주 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.11
This study analysed 83 cervical neoplastic patients with HPV-16 DNA detected by PCR by in the patients of the Department of Obstetrics and Gynecology, Kangnam St. Marys Hospital, Catholic University Medical College, comparing the histopathology from Papanicolaou smear and colposcopic guided biopsy, quantitative evaluation of HPV DNA by Hybrid Capture test and the preservation of HPV-16 E2 DNA with PCR. Our analysis revealed: 1. Atypical transformation zone seen by colposcopy was divided into three groups, one where zone covered less than 25%, one between 25~50% and one greater than 50%. When compared with histopathologic findings, CIN and CIN III were present in 31%(10/32), 31 %(8/26), and 75%(18/24) respectively. 2. Comparing of cervical histopathologic results with HPV DNA amounts, viral titer greater than 5.0 was found in non-specific cervicits(13.3%; 4/30), HPV+CIN I(31.3%; 5/16) and CIN II + III(69.4%; 25/36). 3. Comparing of cervical histopathologic results with the preservation of intact HPV-16 E2 DNA using PCR, E2 DNA was present in non-specific cervicitis(7%; 2/30), HPV+CIN I(0%;0/16), and CIN III + III(47%; 17/36). 4. The presence of HPV-16 E2 DNA was compared with HPV DNA titer. When HPV-16 E2 DNA was present, titer was greater than 5.0 in 89%(17/19), while HPV-16 E2 DNA was absent, titer was greater than 5.0 in only 27%(17/63). Our analysis revealed that high HPV DNA titer and complete HPV-16 E2 DNA correlated well with advanced grade of CINs. In conclusion, this result suggest that HPV DNA titer and HPV-16 E2 DNA presence can clinically assist in predicting the prognosis of CINs.