Size-exclusion chromatography법에 의한 식품 중 알긴산프로필렌글리콜 분석법 확립

정은정,최유정,이근영,윤상순,임호수,김용석,Jeong, Eun-Jeong,Choi, Yoo-Jeong,Lee, Gunyoung,Yun, Sang Soon,Lim, Ho Soo,Kim, Yong-Suk 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.5

HPLC-size-exclusion chromatography에 의해 가공식품에서 알긴산프로필렌글리콜의 함량을 분석하는 방법이 개발되었다. 알긴산프로필렌글리콜을 분석하기 위해 GF-7M HQ column과 LT-ELSD detector가 선정되었다. 알긴산프로필렌글리콜 분석을 위한 전처리 조건으로는 $20^{\circ}C$에서 150 rpm으로 3시간 동안 추출하는 방법이 선정되었다. 알긴산프로필렌글리콜을 5 농도(300, 500, 700, 1,000, and 1,500 mg/kg) 범위에서 검량선을 작성한 결과 직선성($R^2$)은 0.9873으로 측정되었다. HPLC system에 의한 알긴산프로필렌글리콜 분석시 검출한계(LOD) 및 정량한계(LOQ)는 각각 171.43 mg/kg 및 519.50 mg/kg이었다. Size-exclusion chromatography에 의해 얻은 회수율 및 변동 계수(coefficient of variation)는 각각 86.1~110.4% 및 4.1~13.5%이었다. 본 연구에서 개발된 HPLC-size-exclusion chromatography system을 적용하여 134 품목의 가공식품에서 알긴산프로필렌글리콜 함량을 분석하였다. 이 결과들은 이 방법이 가공식품에서 알긴산프로필렌글리콜 함량을 분석하는데 적용할 수 있는 방법이라는 것을 나타낸다. An analytical method for determination of propylene glycol alginate (PGA) in food products was developed by HPLC-size-exclusion chromatography. The GF-7M HQ column and LT-ELSD detector were determined by considering the instrumental analysis conditions for PGA analysis. The pretreatment method for the analysis of PGA was suitable for 3 hr extraction at $20^{\circ}C$ and 150 rpm according to the extraction temperature. Linearity ($R^2$) for the analysis of PGA was 0.9873 at calibration curve range of 300, 500, 700, 1,000, and 1,500 mg/kg (5 points). The limit of detection and limit of quantification of PGA on HPLC system was 171.43 and 519.50 mg/kg, respectively. The accuracy and coefficient of variation obtained by size-exclusion chromatography were 86.1~110.4% and 4.1~13.5%, respectively. By applying the HPLC-size-exclusion chromatography system, it was possible to analyze the contents of PGA in 134 different types of food products.

양파식초의 발효제조 및 제품의 생리활성

정은정,박혜진,차용준,Jeong, Eun-Jeong,Park, Hye-Jin,Cha, Yong-Jun 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.6

Commercialized production of onion vinegar, which has biological activities formed through alcohol and acetic acid fermentation, requires standardization. The objective of this study was to determine optimal conditions of sugar contents ($11{\sim}15^{\circ}Brix$) and agitation rate (100~300 rpm) of fermenter in the alcohol-acetic fermentation for producing onion vinegar. The alcohol and total acidity contents increased, whereas contents of total sugars decreased during alcohol fermentation. Contents of alcohol of 13 and $15^{\circ}Brix$ reactants were about 8% in 36 hr and total acidities of all samples were below 0.2% in 60 hr. During acetic fermentation, total acidity increased with highest value at 9 days (3.2% in 100 rpm), 10 days (4.1% in 200 rpm) and 8 days (4.3% in 300 rpm), respectively. From these results, sugar contents ($13^{\circ}Brix$) were measured for alcohol fermentation and agitation rate (300 rpm) for fast fermentation method of vinegar. The contents of total phenols, flavonoids and quercetin in onion vinegar were 33.3 mg/100 g, 3.0 mg/100 g and 2.0 mg/100 g, respectively. Onion vinegar showed an antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Escherichia coli and Enterobacter aerogenes. Antioxidant effect of onion vinegar was 26.23% in DPPH radical inhibition and 58.58% in superoxide dismutase like activity, respectively. Fibrinolytic activity was 1.51 plasmin unit/mL in onion vinegar. In conclusion, onion vinegar processed by alcohol and acetic fermentation had nutritional values and potential biological activities.

오미자 첨가 양파초음료 제조 및 항균·항산화 활성

정은정,차용준,Jeong, Eun-Jeong,Cha, Yong-Jun 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.1

Onion vinegar, which has an undesirable flavor and taste formed through alcohol and acetic acid fermentation, possesses additives that can improve sensory quality. Thus, the objective of this study was to present an optimized blending ratio using response surface methods for an onion vinegar beverage by adding Omija extracts. This study was performed to formulate an Omija-onion vinegar beverage (OOVB) and investigate its antioxidant properties and antimicrobiological effects. The experimental design was conducted using an optimal mixture model of response surface methodology which generated eighteen experimental trials with overall acceptance as the responses. According to the statistical analyses, OOVB demonstrated a ratio containing onion vinegar, water, brown sugar, apple extracts and Omija extracts of 10, 72.3, 4.4, 12.2 and 1.1 (weight ratio), respectively. The OOVB revealed desirable nutrition values (phenolics compounds 19.3 mg/100 g, total flavonoids 3.1 mg/100 g, quercetin 1.9 mg/100). The OOVB displayed antibacterial effects in Gram negative Enterobacter aerogenes, Escherichia coli, Salmonella typhimurium and Gram positive Staphylococcus aureus. The findings revealed that OOVB was 18% in DPPH radical inhibitionand 11% in superoxide dismutase-like activity thus, OOVB has nutritional value and good quality as well as potential biological activities for functional beverages.

딸기주 발효를 위한 효모 선발과 살균 방법의 비교 및 발효 중 이화학적 특성의 변화

정은정(Eun-Jeong Jeong),김용석(Yong-Suk Kim),정도연(Do-Youn Jeong),신동화(Dong-Hwa Shin) 한국식품과학회 2006 한국식품과학회지 Vol.38 No.5

고려중기 경주의 邑基 이전과 景觀

정은정(Jeong - Eun Jeong) 한국사학회 2012 史學硏究 Vol.- No.106

신라 왕도시절 통치의 핵심영역인 경주 都內는 고려 태조이래 邑內?外로 분리된 데다, 현종 3년에는 邑內를 중심으로 성곽을 축성하게 되었다. 당시의 축성은 현종 2년 동여진 침입에 대비한 방어차원이긴 하나, 차후 경주의 영역정비와 新邑基건설에 견인차 역할을 했다. 경주의 서북방면에 치우친 초기금성터에 읍기가 건설되기 전까지는 월성일대가 舊邑基지역으로 기능했다. 경주의 읍기가 월성방면에서 초기금성터 쪽으로 옮아가는 양상은 발굴자료를 통해서 확인가능하다. 대체로 고려전기에는 월성일대에서, 고려중기 이후로는 초기금성터에서 관련 유물이 집중되고 있다. 한편 현종 이후 경주 新邑基건설은 성곽 축성 즉시 착수되지는 못했다. 경주세력의 제어와 공사 기일의 문제 등 제반 여건으로 인해 기존 월성일대의 기간시설물을 활용하는 절충 시기가 잠시 있었다. 이때 경주 舊邑基에 해당하는 월성일대 사찰과 재정?유통시설이 활용되었다. 문종전후해서 경주는 점차 지방도회로서 국가가 강제하는 경관을 구축해 나갔다. 경주 신읍기인 초기금성터 주변으로는 강한 구심력을 보이는 資福寺와 客舍가 배치된 것이다. 新邑基의 관향은 외방관도와 배후촌락과의 통행을 참작해서 결정되었다. 경주 신읍기의 도로는 각 성문을 연결하는 십자형태로서 서북방면의 慶州道와 만난다. 경주도의 진행방향에 맞춰 인근의 속현도 신읍기 외곽에 차례대로 배속되었다. 또한 新邑基에서 서쪽?북쪽 방면으로는 통행편의를 위해서 細路가 신설되거나 자연 촌락의 생성이 두드러졌다. 경주 新邑基에서의 도로는 월성일대의 舊邑基에 비하면 상대적으로 도로의 개설연한도 늦고 노폭도 좁은 편이다. 기존 왕경대로를 개?보수하던 차원과는 달리 新邑基서?북쪽으로는 새로이 자연적인 ‘길’이 만들어진 것이다. 이들 小路는 이전부터 있어왔던 촌락과의 주요통로와 서북방면에 신설된 촌락 사이의 연결을 긴밀히 하는 방향으로 전개되었다. 요컨대 현종 3년 초기금성터 방면에 성곽이 始築되고 난 후 문종 전후해서 경주 新邑基지역은 국가차원의 권위체계 강제와 이에 추수하는 변화로 인해 차츰 지방도회로서의 전형을 갖추게 되었다. In the third year of King Hyeonjong, a castle for administrative authorities was built and a new Eupgi was constructed in the Geumseong districts of Gyeongju. The government office remains in the former part of the Goryeo Dynasty were excavated in the whole area of Wolseong in Gyeongju, and the subsequent relics were unearthed in the Eupseong districts. Since 1012, however, the government-controlled market and granary in the whole area of Wolseong had been continuously used until the construction of the new Eupgi was completed. The townscape of the new Eupgi had been controlled at national level since the reign of King Munjong. And Gyeongjudo(慶州道), an outer arterial road, was repaired. Through the arrangement of Jaboksa(資福寺) and the installation of Gaeksa(客舍), Gyeongju began to take the shape of a provincial town. The new Eupgi was located to the northwest, and its newly-organized villages stood close together in the northwestern area of Gyeongju, in the light of the connection to Gaegyeong. Because the idea of geomancy such as Josan (朝山) Jusan(主山) wasn"t settled down until then. The narrow path in the new Eupgi was constructed for the sake of the close linkage with the main streets in the old villages. Gyeongju took the typical shape as a provincial town by the government-controlled townscape improvement and other small change in the mid-Goryeo Dynasty.

수박의 품종별 이화화적 성분비교

정은정(Eun-Jeong Jeong),신동화(Dong-Hwa Shin),홍선표(Sun-Pyo Hong),은종선(Jong-Seon Eun) 한국생물환경조절학회 2007 한국생물환경조절학회 학술발표논문집 Vol.16 No.2

반 데르 퀘켄 영화 형식의 해방적 성격 -영화 <존재의 얼굴(Face Value)>(1991)을 중심으로-

정은정 ( Eun Jeong Jeong ) 한양대학교 현대영화연구소 2011 현대영화연구 Vol.7 No.2

Johan Van Der Keuken(JVDK), who is a Dutch documentary filmmaker, has filmed several impressive documentaries. The themes of his films are quite extensive, but he has always been interested in political and existential problems such as human alienation in the capitalistic societies, poverty of the Third World, diaspora of minority groups. In order to deal with these themes, he has always searched for a new form of film. And his experiment in film form aims at liberation of image and sound from meaning or reality. JVDK`s <Face Value(1991)> is a film which is about people who live in 5 countries of Europe. Each character in this film, who is from many different racial, religious, economic-social groups, are expressed in the form of face close-up, voice-over, and collage(polyphony). And in the end, the film finally makes a big collage-portrait of europe which is made of many small portraits. In this way the film becomes a kind of ``Anthropology of Face``. In the film, JVDK breaks the conventional film grammar and try experimental forms of film such as polyphonous narratives, a dialectical collage of face close-up and voice-over, black screen, and free camera works etc. And by these new ways of filming, he liberates images and sounds. The liberation of image and sound makes the film a open space which can create various meanings endlessly. JVDK`s film form of liberation is an attempt to give film the autonomous power to create meanings endlessly by liberating image and sound.

위암환자에서 p53단백 및 c-erbB-2 단백발현과 예후인자로서 활용

정은정(Eun-Jeong Jeong),하우송(Woo-Song Ha),박순태(Sun-Tae Park),이영준(Young-Jun Lee),장종연(Jong-Yeun Jang),조영현(Young-Hyeon Cho),이영재(Yong-Jae Lee),정민화(Min-Hwa Jeong) 대한외과학회 1998 Annals of Surgical Treatment and Research Vol.54 No.5

변온숙성에 의한 명태(Theragra chalcogramma)식해의 품질유지

정은정 ( Eun Jeong Jeong ),김훈 ( Hun Kim ),차용준 ( Yong Jun Cha ) 한국수산과학회 2015 한국수산과학회지 Vol.48 No.3

We sought to extend the shelf-life of Alaska pollack Theragra chalcogramma sikhae while maintaining quality. We compared the chemical, microbiological, and organoleptic characteristics of sikhae prepared under four different conditions. Control fish were fermented at ambient temperature (21±2℃); other samples were stored at 5℃ after fer-mentation at ambient temperature for 36 h (A1), 60 h (A2), and 84 h (A3). Volatile basic nitrogen and amino-nitrogen levels, and total acidity increased with fermentation time in all samples, but the pH fell, attaining a relatively lower level in the control than in other samples. Over 90% of all viable cells were lactic acid-producing bacteria; this pro-portion did not change significantly during fermentation. In terms of texture, only hardness was affected by fermenta-tion. The hardness of the control fell more rapidly than did that of the other samples. In terms of sensory evaluation (the acceptance test and quantitative descriptive analysis [QDA]), A2 was superior to other samples after fermenta-tion for different times; A2 maintained limited salability (6 points on the relevant index) for up to 17 days of storage.

12·14세기 경주의 도시 유통권 - ‘線’ 단위 도시 근린성 확장을 중심으로 -

정은정(Jeong, Eun-Jeong) 효원사학회 2019 역사와 세계 Vol.- No.55

고려 界首官이 가지던 본질적 기능 가운데 문서행정․사법권․재정운영의 기본단위로서 역할은 상위의 道制 시행이 가속화되면서 일부 중복되었다. 물류수송의 문제에 봉착해서는 道制가 정착하는 후기 부터 재화의 이동반경이 계수관의 관할영역을 상회하여 부분적으로 소위 越境하는 이동이 간파된다. 이 경향은 고고자료에서도 지지된다. 계수읍․주읍․속읍의 각 치소를 뛰어넘는 재화의 흐름은 추후에 생활물자의 유통과 맞물릴 경우 도시 近隣性을 증폭시키는 기제로 작용한다. 12세기 전후 전반적인 기층사회의 변화 가운데 항쟁을 선도하던 경주는 중후기에 이르면 성종대 설정한 순주경의 지위를 상실하였다. 경주의 위상이 격하된 위에 고려 왕조를 전복하려던 움직임에다 원간섭의 폐해와 뒤이은 왜구침입으로 경주 촌락내부의 교란은 매우 심각하였다. 수습하는 중앙정부의 조치는 경주세력의 중앙정계 진출을 차단하면서 경주권역을 축소시키려는 강경일변도였다. 경주는 국초 東京의 위상을 회복하지 못한 채 고려말까지 국토의 변방으로 인식되기에 이르렀다. 중앙에서 괴리된 변방이라는 인지는 오히려 경주 지역내부의 결속과 귀속감을 다지는 계기가 되어주었다. 중앙에 대비되는 경계․他者性은 국가틀을 벗어날 경우 민간부문의 성숙에 연동하여 더욱 강하게 드러내는 속성이 있다. 관련하여 12․14세기 경주에서도 국가 물류망에 생활물자의 유통망이 중첩되거나 일부 새로이 만들어지기도 한다. 경주권역에서 출토가 집중되는 해당시기의 분묘․와요․생활물자의 이동을 밝혀 행정경계를 상회하는 물화유통망을 추적한 결과 경주․울산․포항․대구의 기존 경주계수관 영역을 넘어 타 계수관․道 관할단위까지 동일기호도가 반영된 도기․청동제품․기와를 위시하여 어염․미곡의 생필품이 거래되고 있다. 14세기 가까워질수록 지역색깔을 지닌 域內 생산물질이 경주 계수관 단위를 넘어서 타 권역으로 유입되기도 한다. Gyeongju saw an inter-regional exchange of goods go beyond the administrative boundaries of Gyesugwans between the twelfth and the fourteenth centuries. While several Geysugwans, as urban entities, were connected, the exchange network of goods passed through the transitional stage in which the network covered a larger area afterwards. The trade of products, by the fourteenth century, consumed by the Hyangri and lower classes, went farther than the previous market area. Metal items, pottery and plain roof tiles with certain patterns, which were traded around the Gyeongju Gyesugwan, were discovered beyond the region in this century. This reveals the transboundary exchange of goods. The government gained a strong and sophisticated sense of control over the nation-wide network by controlling the trading network. The growth of the marketing area, led by the product exchange between Gyesugwans, was accompanied by the expansion of the daily living area. Gyeongju in the Goryeo period lost its political importance as the capital of the previous dynasty, Silla. However, various changes in local boundaries partly contributed to the growth of product exchange. The places of production and of consumption which were scattered throughout the region started being connected with each other from the twelfth century as bridges were built along byroads between arterial roads that linked one big town to another. In addition to the development of infrastructure, the improvements in the distribution system and facilities made the enlargement of the exchange network. Goods flow transferred between Gyeongju and other Gyesugwan regions from the twelfth to the fourteenth centuries. Province-based local administration was settled after the central government took over the management and control of the long-distance trade between Gyesugwan regions. Gyeongju played a significant role as the central commercial region in the Gyeongsang region, covering the eastern and southern parts of the province.