Genetic Study of Segregation - Distorter(SD)of Korean Natural Populations of D . melanogaster

정용재,강문주,강순자 한국유전학회 1986 Genes & Genomics Vol.8 No.1

In order to see if the higher temperature (30℃) affected on the SD action, three SD strains were raised at different temperatures (25℃ and 30℃) and then emerged flies were examined for the k values. The results indicated that 1) no significant difference of the k values were detected between the two different temperatures (25℃ and 30℃), 2) no variations of the k values were found from the continuous treatment of the higher temperature (30℃) on the SD flies and 3) the sensitivity of temperature on the SD action seemed to be positive but the effect of temperature might be not heritable.

韓國産 초파리 自然集團의 4酵素 因子頻度의 變異에 관한 硏究

鄭瑢載,姜順子,張惠媛 梨花女子大學校 韓國生活科學硏究院 1985 韓國生活科學硏究院 論叢 Vol.36 No.-

한국산 초파리 집단의 유전생화학적 체계를 수립하기 위하여 11개 지역의 한국산 노랑초파리(D.melanogaster)의 자연집단(서울-봉천동, -청담동, -역삼동, 경기도-고양, -김포, -수원, -안양, 인천, 경북-영천, -경주 및 전남-강진)을 대상으로 4가지 효소(MDH-1, MDH-2, ACPH 및 ME)의 인자형과 인자빈도를 starch gel 전기영동법에 의하여 조사 분식한 길과는 다음과 같다. 1. 4효소 중 MDH-2와 ME는 monomorphic하고 집단간에 변이가 없으며 나머지 MDH-1과 ACPH는 polymorphic하며 집단간에 차이를 보인다. 2. Polymorphic한 효소의 heterozygosity는 MDH-1이 13.73%로서 ACPH의 6.91%보다 높은 값을 보인다. 3. 인자형빈도에 있어서는 ACPH에서 FF인자형(0.9209)이 전 집단에 걸쳐서 우세하게 분포하고 있으며, MDH-1은 SS인자형(0.8518)이 높은 빈도로 나타났으며 MDH-2와 ME는 SS인자형만 나타났다. 4. F인자가 ACPH에서 높은 빈도(0.9555)로 나타났으며 이와는 반대로 MDH-1은 S인자빈도(0.9205)가 높게 나타났으며 MDH-2와 ME는 S인자만으로 고정되어 있다고 볼 수 있다. In order to establish a biochemical genetic system of Drosophila populations in Korea, four enzyme alleles of natural populations of D. melanogaster from 11 localities in Korea were analyzed by means of starch gel electrophoresis. The results obtained were as follows: 1. MDH-1 and ACPH were found to be polymorphic and others, MDH-2 and ME, to be monomorphic in the 11 natural populations of D. melanogaster in Korea. 2. Heterozygosity was calculated to be 13.73% for MDH-1 and 6.91% for ACPH, respectively. 3. The FF genotype of ACPH alleles was most frequently distributed but the SS genotype showed the highest frequency in MDH-1. 4. The F gene frequency was found to be higher than the S gene in ACPH, whereas the S gene frequency was found to be higher than the F gene in MDH-1 and the S gene was fixed in MDH-2 and ME alleles.

韓國産 노랑초파리(Drosophila melanogaster)의 羽化率에 미치는 몇 가지 農藥의 影響에 대한 硏究

鄭瑢載,李明鮮 梨花女子大學校 韓國生活科學硏究院 1980 韓國生活科學硏究院 論叢 Vol.25 No.-

최근 농약의 개발과 사용량의 증가로 자연 환경이 오염되어가고 있으므로 이에 대한 관심이 높아지고 있다. 농약의 게속적인 사용으로 인하여 과수 주위에 잘 모이고 번식하는 초파리에게도 농약으로 인한 영향이 있을 것에 주목하여 현재 일반적으로 사용되고 있는 유기인계 살충제인 parathion, methidathion(supracide), metrifonate(trichlorfon, dipterex)가 노랑초파리(Drosophila melanogaster)의 우화에 미치는 영향을 조사함으로써 농약의 공해문제를 검토하기 위하여 5계통의 초파리(Oregon-R, 대전, 부산, 삼성혈, 신촌-Ⅰ)를 각 농약의 0.0, 1.0×10^-4, 1.4×10^-4, 2.0×1.0^-4, 2.5×10-4, 3.3×10^-4 and 5.0×10^04% 농도 별로 첨가시킨 메디아에 산란시켜 사육한 후 초파리의 우화율을 조사 검토한 결과는 다음과 같다. 1) 3가지 농약(parathion, methidathon, me-trifonate)의 처리는 노랑초파리의 5계통 간에 어느 농도에서나 우화율의 유의적인 차를 볼 수 없다. 2) 3가지 농약의 농도 간에는 5계통 모두 우화율의 유의적인 차이를 보이고 있으며, 농도가 증가함에 따라 우화율이 저하되어 초파리 우화에 억제적 효과를 나타낸다. In order to see of the agrochemicals affected on the emergence rates of Drosophila melano-gaster, the effects of the three agrochemicals on the emergence rates of the five strains of Dro-sophila melanogaster (Oregon-R, Daejeon, Busan, Samseonghyeol, and Sinchon-Ⅰ) were examined. The agrochemical media were prepared by adding them at several concentrations (0.0, 1.0×10^-4, 1.4×10^-4, 2.0×1.0^-4, 2.5×10-4, 3.3×10^-4 and 5.0×10^04%) to the stand-ard agar-yeast-corn meal media for the present experiment. The results are given below: 1. The emergence rates are not significantly different among the five strains of Drosophila melanogaster in the tratment of three kinds of agrochemicals. 2. The significant difference in the emergence rates of Drosophila melanogaster was found among the concentrations of all the agrochemicals used in the present experiment; it decreases as the concentration of agrochemicals increases.

전시-P-45 : 송광사 목조건축물의 흰개미 손상도 조사 연구

정용재,김대운,정선혜,이민영,김시현 韓國木材工學會 2013 한국목재공학회 학술발표논문집 Vol.2013 No.-

韓國人 集團의 胎盤內 酵素 多型現象에 對한 硏究

鄭瑢載,姜順子,柳貞花 梨花女子大學校 韓國生活科學硏究院 1988 韓國生活科學硏究院 論叢 Vol.42 No.-

한국인 집단의 태반내 효소의 다형현상을 조사하기 위하여 esterase D(ESD), alkaline phosphatase(ALP)와 acid phosphatase(ACP)의 효소 3종을 서울 거주 임산부를 대상으로 하여 starch gel 전기영동법으로 분석한 결과는 아래와 같다. 1. ESD isozyme을 ESD^1과 ESD^2의 2개의 대립인자가 발견되었으며 ESD^1과 ESD^2의 빈도는 각각 0.663과 0.337로 나타났다. 그리고 rare allele과 variant 유형은 발견되지 않았다. 2. 사람의 태반에서 나타나는 ALP isozyme은 간, 골격, 신장의 ALP_L과 내장의 ALP_I 좌위와는 다른 PL 좌위로 지배됨이 발견되었다. 3. PL isozyme은 PL^1, PL^2와 PL^3의 3개 대립인자로 결정되는 6개 공통 표현형과 1개의 rare 표현형(PL 4-1)이 발견되었으며 PL 좌위의 대립인자 빈도는 PL^1, PL^2, PL^3와 PL^rare가 각각 0.727, 0.081, 0.191 및 0.001로써 나타났다. 4. 태반에서 나타나는 ACP isozyme은 적혈구의 ACP_1과는 다른 ACP_2와 ACP_3 좌위로 결정되며 본실험에서는 ACP_3 좌위에서 5개체의 variant유형(ACP_3 2-1)이 발견되었다. Polymorphism of placental enzyme(ESD, Alp and ACP) was studied by means of starch gel electrophoresis in unrelated placental donors living in Seoul, Korea and the results were obtained as follows: 1. The two alleles of EDS enzyme, ESD^1 and ESD^2 were found and the gene frequencies of ESD^1 and ESD^2 were detected to be 0.663 and 0.337, respectively. And no rare alleles and variant type were found in the present study. 2. The ALP phenotypes in human placenta could be explained by the different locus(PL)with three alleles distinct from the ALP_L(in liver, bone and kidney) and ALP_I(in intestine). 3. As to PL isozyme, there were three aleles, PL^1, PL^2 and PL^3. One of the variants(PL 4-1) was found in the present study. The gene frequencies of alleles were found to be 0.727 for PL^1, 0.081 for PL^2, 0.191 PL^3 and 0.001 for PL^rare. 4. The ACP phenotypes in placenta could be explained by the ACP_2 and ACP_3 locus distinct from the ACP_1 (in erythrocyte) and the five variants(ACP_3 2-1) at ACP_3 locus were found.

인, 황이 도핑된 2D 표면구조 전이금속 촉매 Ni(Co,Fe)PS의 합성과 물분해 촉매로서 이용

정용재,노효균,용기중 한국표면공학회 2021 한국표면공학회 학술발표회 초록집 Vol.2021 No.12

Effect of Intradiscal Monopolar Pulsed Radiofrequency on Chronic Discogenic Back Pain Diagnosed by Pressure-Controlled Provocative Discography: A One Year Prospective Study

정용재,안상호,이동규,조윤우 대한재활의학회 2012 Annals of Rehabilitation Medicine Vol.36 No.5

Objective To investigate the efficacy and safety of percutaneous intradiscal monopolar pulsed radiofrequency (PRF) in patients with chronic disabling discogenic back pain.Method Twenty-six subjects (7 males; mean age 43.2 years) with chronic back pain refractory to active rehabilitative management were recruited. All subjects underwent MRI for evaluation of Modic changes, and monopolar PRF (20 min at 60 V) at the center of target lumbar intervertebral disc confirmed by pressure-controlled provocative discography. Clinical outcomes were measured by the visual analogue scale (VAS), Oswestry disability index (ODI), and sitting tolerance time (ST) for 12 months after treatment. Successful clinical outcome was described as a minimum of 2 point reduction in VAS compared with the baseline at each follow-up period.Results The mean VAS for low back pain reduced significantly from 6.4±1.1 at pre-treatment to 4.4±1.9 at 12 months (p<0.05). The mean ODI score was 47.3±15.4 points at pre-treatment and 36.7±19.5 at 12 months (p<0.001). The ST was 27.8±20.4 minutes at pre-treatment and 71.5±42.2 at 12 months (p<0.001). However, successful clinical outcome was achieved at 58%, 50%, and 42%, measured at 3, 6, and 12 months post-treatment. There were no significant relationship between the clinical outcome and Modic changes; no adverse events were recorded.Conclusion The results demonstrated that the application of intradiscal monopolar PRF might be relatively effective but limited; successful intervention for chronic refractory discogenic back pain is needed. To achieve the optimal outcome through intradiscal PRF, we suggested further studies about stimulation duration, mode, and intensity of PRF.

한국산 초파리 집단의 유전 생화학적 연구: 노랑 초파리의 $\alpha$-Glycerophosphate dehydrogenase allele에 대하여

정용재,한영수,정영란 한국통합생물학회 1982 동물학회지 Vol.25 No.3

한국산 초파리집단의 유전적 특성을 생화학적으로 분석하여 분자유전학적 측면에서 집단 유전학적 체계를 확립하기 위하여 한국산 노랑초파리 (Drosophila melanogaster)의 11개 지역의 자연집단 (청량리, 등촌동, 신촌, 용산, 부천, 춘천, 대전, 대구, 광주, 나주 및 노화도)의 $\\alpha$-glycerophosphate dehydrogenase (\\alpha-GPDH) 인자형을 agarose gel 전기영동법에 의하여 조사 분석한 결과는 다음과 같다. 1. 한국산 노랑초파리 11가지 자연집단에서 $\\alpha$-GPDH allele는 상당한 다형현상을 이루고 있다. 2. $\\alpha$-GPDH allele의 heterozygosity는 $45\\sim50%$로서 상다히 높은 값을 보여주고 있다. 3. $\\alpha$-GPDH allele의 FF 인자형의 빈도는 SS인자형과 비슷하나 FS 인자형보다는 낮은 값을 보이고 있다. 4. $\\alpha$-GPDH allele의 F, S 두인자가 거의 비슷한 빈도를 보이고 있다. In order to establish a biochemical genetic system in Drosophila populations in Korea, the $\\alpha$-glycerophosphate dehydrogenase alleles of eleven natural populations of D. melanogaster in Korea were examined by means of agarose gel electrophoresis. The results obtained are presented below: 1. $\\alpha$-Glycerophosphate dehydrogenase ($\\alpha$-GPDH) allele is scored for eleven natural populations of D. melanogaster in Korea, resulting that $\\alpha$-GPDH is found to be widely polymorphic for two electrophoretic variants. 2. The heterozygosity of $\\alpha$-GPDH is calculated as $40\\sim50%$. 3. The frequency of the FF genotype of $\\alpha$-GPDH is found to be roughly same as the SS genotype, but less than the FS genotype. 4. The F gene of $\\alpha$-GPDH is distributed almost frequently as the S gene.

태음조위탕(太陰調胃湯) 기원(基源), 변천과정(變遷過程) 및 구성원리(構成原理)

정용재,고병희,이의주,Jeong, Yong-Jae,Koh, Byung-Hee,Lee, Eui-Ju 사상체질의학회 2009 사상체질의학회지 Vol.21 No.2

1. Objects : This paper was written in order to understand of Taeumjowitang(太陰調胃湯). 2. Methods : We analysis prescription's combination of Taeumin's experior diseases through pathology and new prescription in DongyiSuseBowonSasangChoBonKywun(東醫壽世保元 四象草本卷), DongyiSuseBowon GabObon(東醫壽世保元 甲午本), DongyiSuseBowon ShinChukBon(東醫壽世保元 辛丑本). 3. Results and Conclusions : 1) The origin of Taeumjowitang(太陰調胃湯) is BoPyeSaengMacTang(補肺生脈湯) which is combination of SaengMacSane(生脈散) and SanYakHwaWiJun(山藥和胃煎). 2) BoPyeSaengMacTang(補肺生脈湯) was changed to GilGyungSaengMacSan(桔梗生脈散) in DongyiSuseBowon GabOBon(東醫壽世保元 甲午本). GilGyungSaengMacSan(桔梗生脈散) was changed to HanDaYulSoTang(寒多熱少湯) and Taeumjowitang(太陰調胃湯) in DongyiSuseBowon ShinChukBon(東醫壽世保元 辛丑本). 3) Taeumjowitang(太陰調胃湯) is combination of BoPyeWonTang(補肺元湯) which help Exhale Dispersing Qi of Lung, and adlay(薏苡仁), dry nut(乾栗) and radish seed(羅?子) which help Stomach Qi, calamus(石?蒲) which regular Qi, ephedra(麻黃) which is exhale experior cold Qi.

2022 개정 초등 과학과 교육과정 시안 개발 경과와 주요 내용

정용재,문양희,전성수,강석진,김성호,임재근,소금현,신원섭,박철진,오현석,권유지,한제준,이성희,하지훈 한국초등과학교육학회 2022 한국초등과학교육학회 학술대회 Vol.82 No.-