Agrobacterium tumetaciens 및 Ti Plasmid DNA 에 의한 감자 ( Solanum tuberosum L . ) 괴경세포의 형질전환

정상호,심웅섭 ( Sang ho Chung,Woong Seop Sim ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.4

In the present study, transformation of potato tuber cells by Agrobacterium tumefaciens and Ti plasmid treatment was studied. The results are as follows: Tumors were induced in potato tuber discs by infection with Agrobacterium tumefaciens KU-12. Whole plants were regenerated from the tumors on hormone-free medium. It was identified that the lysopine dehydrogenase (LpDH) activity was present in the tumor-derived plants. A total of 165 calli were obtained from Ti-plasmid DNA(pTi-12)-treated potato protoplasts on hormone-free medium and a few of them formed a root. Each of the non-rooted and rooted callus was randomly selected and tested for the presence of LpDH activity. Both of them were ocotopine positive, indicating that they were transformants. In order to analyze the T-DNA region maintained in transformed plant genome, the DNA-DNA hybridization experiment was performed. As a result, it was found that Sma I fragments of Ti plasmid (pTi-12) 13.4 kb, 3.7 kb, and 4.75 kb in size, were completely contained within T-region, and each fragment of 9.7kb and 4.55 kb was joined to opposite ends of T-region. Also, it was identified that Sma I fragments which were contained within T-DNA region were covalently linked together within the pTi-12 DNA molecule.

Transformation of Potato (Solanum tuberosum L.) Tuber Cells with Agrobacterium tumefaciens and Ti Plasmid DNA

정상호,심웅섭,Chung, Sang-Ho,Sim, Woong-Seop 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.4

본 연구에서는 Agrobacterium tumefaciens 및 Ti-plasmid에 의한 감자 괴경세포의 형질전환 여부를 조사하여 다음과 같은 결과를 얻었다. Agrobacterium tumefaciens KU-12의 감염에 의하여 감자 괴경의 원반에 tumor가 형성되었으며, 이 원반을 호르몬이 없는 배지에서 배양한 결과 tumor에서 완전한 식물체가 재생되었다. 또한 tumor에서 재생된 식물체 내에는 lysopine dehydrogenase(LpDH) 활성이 있음을 확인하였다. 감자의 원형질체에 Ti-plasmid DNA(pTi-12)를 처리하여 호르몬이 결여된 배지에서 배양한 결과, 165개의 callus를 얻었으며, 그 중 일부에서는 뿌리가 형성되었다. 뿌리를 형성한 callus와 형성하지 않은 callus을 임의로 선택하여 LpDH 활성을 조사한 결과 둔 경우 모두 octopine을 합성함으로 형질전환체임을 확인하였다. 형질 전환된 식물의 genome내의 T-DNA 부위를 분석하기 위하여 DNA-DNA 혼성화실험을 성시한 결과, pTi-12 DNA내에 존재하는 T region내에는 Sma I fragment들 중 분자량이 134 kb, 37 kb 및 4.75 kb인 것이 완전히 내포되어 있으며, T region의 양쪽 끝은 분자량이 각각 9.7 kb와 4.55 kb인 Sma I fragment에 연관되어 있음을 알 수 있었고, 또한 T-DNA에 내포된 Sma I fragment들은 pTi-12 DNA 분자내에서 서로 연결된 상태로 존재하고 있음을 확인하였다. In the present study, transformation of potato tuber cells by Agrobacterium tumefaciens and Ti plasmid treatment was studied. The results are as follows: Tumors were induced in potato tuber discs by infection with Agrobacterium tumefaciens KU-12. Whole plants were regenerated from the tumors on hormone-free medium. It was identified that the lysopine dehydrogenase (LpDH) activity was present in the tumor-derived plants. A total of 165 calli were obtained from Ti-plasmid DNA(pTi-12)-treated potato protoplasts on hormone-free medium and a few of them formed a root. Each of the non-rooted and rooted callus was randomly selected and tested for the presence of LpDH activity. Both of them were ocotopine positive, indicating that they were transformants. In order to analyze the T-DNA region maintained in transformed plant genome, the DNA-DNA hybridization experiment was performed. As a result, it was found that Sma I fragments of Ti plasmid (pTi-12) 13.4 kb, 3.7 kb, and 4.75 kb in size, were completely contained within T-region, and each fragment of 9.7kb and 4.55 kb was joined to opposite ends of T-region. Also, it was identified that Sma I fragments which were contained within T-DNA region were covalently linked together within the pTi-12 DNA molecule.

감자 ( Solanum tuberosum L. ) 와 담배 ( Nicotiana tabacum L. ) 의 원형질체 배양 및 융합

정상호(Sang Ho Chung),심웅섭(Woong Seop Sim) 한국식물학회 1987 Journal of Plant Biology Vol.30 No.4

The seasonal pattern and the annual magnitude of the internal (or biochemical) and plant-soil cycles of phosphorus in adjacent three different herb plant populations-Helianthus tuberosus L., Artemisia princeps Pampan and Phalaris arundinacea L. -in an old field of the basin in the Mt. Geumoh were investigated. A lot of the phosphorus demand for the three populations was supplied by the withdrawal from below-ground organ in early growing period, by the absorption from soil in mid-growing period and by the withdrawal from stem in later growing period. But in the A. princeps and P. arundinacea populations, a great deal of phosphorus seemed to be absorbed prior to the first withdrawal. The annual amount of the phosphorus flowing through the internal cycle was about 59% of the total phosphorus flow on the organ level in the H. tuberosus population, 41% in the A. princeps population and 32% in the P. arundinacea population, indicating that the tuber plant had the most developed internal cycle system. The annual amount of phosphorus which took part in the plant-soil cycle in the stand of the three populations was in the range of 4.49-5.65 gP m^-2, corresponding to only 3-8% of the extractable phosphorus contained in the soil of 0-20 ㎝ depth The fact that the H. tuberosus population is higher in the extent of internal cycle but smaller in the magnitude of plant-soil cycle and lower in the turnover rate of phosphorus than the other two populations suggests that the growth of H. tuberosus population may be less dependent on soil phosphorus availability than those of the other two populations. The regenerative capacities of protoplasts isolated from potato(Solanum tuberosum L.) tubers and tobacco (Nicotiana tabacum L.) mesophyll tissues were examined, and then their intergeneric protoplast fusion was carried out. The potato tuber-derived protoplasts proliferated into the calli some of which showed rudimentary shoot-like structures, which had not been attempted before from tubers, while the tobacco protoplasts were regenerated into the whole plants. Intergeneric protoplast fusion between potato and tobacco was carried out and the heteroplasmic fusion products were formed. The first cell division of some of them was observed after 5 days of culture.

감자 ( Solanum tuberosum L. ) 괴경의 배양세포로부터 원형질체의 분리

정상호(Sang Ho Chung),심웅섭(Woong Seop Sim) 한국식물학회 1986 Journal of Plant Biology Vol.29 No.1

Protoplasts were isolated from cultured cells of potato (Solanum tuberosum L.) tuber tissue. The ability of callus formation from the cultured cells was higher in cultivars Dejima and Superior than in Shimabara and Irish Cobbler on Lam`s medium. Therefore, the former was used as sources for protoplast isolation. Friable calli were transferred to liquid media and cells in exponential phase were used for protoplast isolation. In both of Dejima and Superios, the yield of protoplasts was high in the enzyme solution of 2% Onozuka cellulase and 1% macerozyme. Also, viability of isolated protoplasts was very good. Thus, it seems that these protoplasts would be applicable to various aims of research.

발아중인 옥수수 종자내에서 RNA 의 생합성에 미치는 GA3 의 효과

정상호(Sang Ho Chung),심웅섭(Woong Seop Sim) 한국식물학회 1983 Journal of Plant Biology Vol.26 No.1

In order to investigate the effects of GA_3 on RNA biosynthesis, the amounts of rRNA and tRNA in germinating maize seeds were measured. The amount of rRNA in the endospermless seedlings was remarkably increased by GA_3 treatment after 48 h of germination, but no effect of GA_3 after 12 h and 72 h of germination was observed. The amount of tRNA was slightly increasedd by GA_3 treatment throughout 24 h to 72 h after germination, but no effect was observed after 12 h of germination. While the amount of rRNA in 0.5㎝ shoots in length was decreased by GA_3 treatment, both of the amount of rRNA were increaed in 1∼1.5㎝ shoots. According to the above mentioned results, it may be suggested that RNA biosynthesis is affected by GA_2 treatment, and that GA_2 participates in the biosysnthesis of rRNA rather than tRNA in germination maize seeds.

고등식물 형질전환용 유전자 운반체 pKCHI 의 개발

정상호(Sang Ho Chung),고경원(Kyung Won Ko),홍주봉(Choo Bong Hong),최인성(In Sung Choi),정태화(Tae Wah Chung) 한국식물학회 1989 Journal of Plant Biology Vol.32 No.1

We have developed a plasmid vector, pKCHl, for the purpose of higher plant transformation. It contains the promoter region of cauliflower mosaic virus 35S transcript (P_35s) and the terminator region of nopaline synthase gene (Tnos) with unique cloning site, Bam HI and Xba Ⅰ, between them. After inserting a foreign gene at the cloning site, P_35s-foreign gene-Tnos cassette can be recovered by using a restriction enzyme Hind Ⅲ.

Convenient Methods for the Extraction and Discrimination of Water-Soluble Plant Pigments

정상호(Sang-Ho Chung),변영호(Young-Ho Byun) 한국콘텐츠학회 2009 한국콘텐츠학회논문지 Vol.9 No.3

식품이나 음료 첨가제로 사용하는 착색제는 소비자가 가공된 식품을 믿고 인정할 수 있는 중요한 요인의 하나로 꼽을 수 있다. 예전에 식품에 안전하다고 다량 사용되었던 인공 착색제나 염료들의 종류는 근래 독성학적 연구 결과 유해하다고 판정되어 그 수가 급격히 줄고 있다. 따라서 안토시아닌이나 베타시아닌과 같이 안전한 천연 색소는 항암성과 항산화성을 갖고 있기에 그 수요가 꾸준히 늘고 있다. 본 연구에서는 수용성 식물 색소인 안토시아닌과 베타시아닌을 에틸 아세테이트, 에틸 에테르 및 클로로포름과 같은 몇가지 유기용매로 간단히 추출할 수 있었다. 또한, 추출한 이 두 가지 주된 식물 색소는 citrate buffer (pH 3.0)를 이용한 아가로즈 겔 전기영동법 하나만으로 간편하고 빠른 시간 내에 전개하여 서로를 구분할 수 있었다. The use of colorants as additives for foods and drinks is a significant factor to food consumers in determining the acceptability of processed foods. In recent years, the number of previously used artificial colorants/dyes suitable for food use has been drastically reduced as a result of toxicological studies. Therefore, the use of natural pigments such as anthocyanins and betacyanins that were known to have anticancer and antioxidant activities is increasingly required. In this study, the water-soluble plant pigments, anthocyanins and betacyanins, were easily extracted with a very simple method using a few organic solvents such as ethyl acetate, ethyl ether, and chloroform. After the extraction of them, these two major plant pigments could be also simply and rapidly separated and discriminated by a solely one-stepped agarose gel electrophoresis in a citrate buffer (pH 3.0).

정전기적 흡,탈착 공정에서의 탄소 전극

정상호 ( Sang Ho Chung ),이재광 ( Jae Kwang Lee ),조이오콘 ( Joey D. Oeon ),손영일 ( Young Il Son ),이재영 ( Jae Young Lee ) 한국공업화학회 2014 공업화학 Vol.25 No.4

인구증가와 산업화로 인한 물의 수요 급증에 따른 제3세대 수처리 기술로써 정전기적 흡·탈착 공정에 대한 연구가진행되고 있다. 정전기적 흡·탈착 기술의 경우, 기존에 사용되는 수처리 방법들에 비해 에너지 소비량이 적으며, 재생시에 2차 오염이 발생하지 않아 차세대 수처리 기술로 주목 받고 있다. 정전기적 흡·탈착 기술에서 이온 제거를 위한전극 물질로는 넓은 비표면적과 높은 전도도를 갖는 탄소 전극이 주로 사용된다. 현재 다양한 탄소 물질로 이루어진전극에 대한 연구가 수행되고 있으며, 특히 비표면적, 기공 분포에 따른 흡·탈착 연구가 활발히 진행되고 있다. 본총설에서는 다양한 탄소 물질 및 기공 분포에 따른 영향을 분석하고, 메조기공과 마이크로기공이 조화를 이루는 최적의 조건을 제시하고자 한다. With the world population’s continuous growth and urban industrialization, capacitive deionization (CDI) has been proposedas a next-generation water treatment technology to augment the supply of water. As a future water treatment method, CDIattracts significant attention because it offers small energy consumption and low environmental impact in comparison to conventionalmethods. Carbon electrodes, which have large surface area and high conductivity, are mainly used as electrode materialsof choice for the removal of ions in water. A variety of carbon materials have been investigated, including their adsorption-desorption behavior in accordance to the specific surface area and pore size distribution. In this review, we analyzed andhighlighted these carbon materials and looked at the impact of pore size distribution to the overall CDI efficiency. Finally,we propose an optimal condition in the interplay between micropores and mesopores in order to provide the best electrosorptionproperty for these carbon electrodes.

Characteristics of the Purple Pigment Compound in Halophytic Plant, Suaeda japonica

Chung sang-ho(정상호) 한국콘텐츠학회 2013 한국콘텐츠학회 종합학술대회 논문집 Vol.2013 No.5

응집지 패들의 운전방향 및 교반속도가 플럭성장과 침전효율에 미치는 영향 평가

이호식 ( Ho Sik Lee ),윤성호 ( Sung Ho Yoon ),정상호 ( Sang Ho Chung ),김영범 ( Young Beom Kim ) 한국물환경학회 ( 구 한국수질보전학회 ) 2010 공동 추계학술발표회 Vol.2010 No.-