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CAM 플라스미드를 함유하는 Pseudomonas putida PpG1에서 TOL 플라스미드이 DNA 재배열
전효곤,조경연,고영희 한국미생물 · 생명공학회 1990 한국미생물·생명공학회지 Vol.18 No.4
접합에 의한 P.putida mt-2의 TOL를 CAM 함유 P.putida PpG1으로 이동시켜 형성된 접합주 P.Putida CST3A는 작아진 TOL(TOL$\Delta$)를 가지고 있었지만, m-toiuate를 분해할 수 있었다. 접합에 의한 이동실험은 CAM에 결합되어 CAM:TO* 플라스미드를 형성하고 있었다. 불화합성 Inc P9군에 속하는 NAH를 CAM:TOL* 과 TOL$\Delta$을 가지는 P.putida CST3A로 이동시키면 TOL$\Delta$의 방출이 관찰되었으나 m-toluate 대사에는 아무런 영향을 미치지 않았다. The TOL plasmid, pWWO, conjugally transferred from Pseudomonas putida mt-2 was dissociated into TOL* and TOL $\Delta$A in P. putidu PpGl carrying CAM plasmid. The TOL* was integrated into the CAM plasmid, and the resulting plasmid was designated as CAM::TOL*. The introduction of NAH plasmid, belonging to Inc P9 incompatibility group, into P. putida CSTBA carrying CAM::TOLt plasmid and TOL A plasmid did not affect m-toluate catabolism, but resulted in expelling the TOL $\Delta$ plasmid.
DNA Rearrangement of TOL Plasmid in Pseudomonas putida PpG1 Harbouring CAM Plasmid
Chun, Hyo Kon,Cho, Kyung Yun,Kho, Yung Hee 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.4
접합에 의해 P. putida mt-2의 TOL를 CAM 함유 P. putida PpG1으로 이동시켜 형성된 접합주 P. putida CST3A는 작아진 TOL(TOL△)를 가지고 있었지만 m-toluate를 분해할 수 있었다. 접합에 의한 이동실험과 불화합성 시험결과, TOL의 m-toluate 분해유전자는 CAM에 결합되어 CAM : : TOL^* 플라스미드를 형성하고 있었다. 불화합성 Inc P9 군에 속하는 NAH를 CAM: : TOL^*과 TOL△을 가지는 P. putida CST3A로 이동시키면 TOL△의 방출이 관찰되었으나 m-toluate 대사에는 아무런 영향을 미치지 않았다. The TOL plasmid, pWWO, conjugally transferred from Pseudomonas putida mt-2 was dissociated into TOL^* and TOL △ in P. putida PpG1 carrying CAM plasmid. The TOL^* was integrated into the CAM plasmid, and the resulting plasmid was designated as CAM::TOL^*. The introduction of NAH plasmid, belonging to Inc P9 incompatibility group, into P. putida CST3A carrying CAM::TOL^* plasmid and TOL △ plasmid did not affect m-toluate catabolism, but resulted in expelling the TOL △ plasmid.
Trichoderma sp. MR-93 균주가 생산하는 Isocyanide 계열의 Melanin 생성 저해물질
이충환,전효곤,정명철,이호재,배경숙,고영희 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.2
미생물로부터 멜라닌 생성 저해물질을 탐색하던 중 mushroom tyrosinase 및 Streptomyces bikiniensis의 melanin 생성을 억제하는 균주를 선발하고, 그 균주가 생산하는 활성물질을 분리정제한 후 구조를 결정하였고 정제된 물질에 대한 활성을 조사하였다. 선발된 MR-93 균주의 형태적, 배양적 특성 등을 조사한 결과 본 균주는 Trichoderma sp.로 분류되어 본 균주를 Trichoderma sp. MR93(KCTC 0114BP)로 명명하였다. 또한 균 배양액으로부터 Diaion HP-20 column chromatography, ethyacetate extraction, Sephadex LH-20 column chromatography 및 HPLC 등을 통하여 melanin 생합성 저해물질 MR-93D를 정제한 후 UV, ^1H-NMR, ESIMS, IR 등의 기기분석을 한 결과 본 물질은 4-hydroxy-8-isocyano-1-oxaspiro[4·4]cyclonon-8-en-2-one로 동정되었다. MR-93D의 mushroom tyrosinase에 대한 IC_50은 0.03 ㎍/㎖이었고, S. bikiniensis의 melanin 생산에 대한 저해는 30 ㎍/paper disc 투여시 35 ㎜ 지름의 저해환을 나타내었다. During the screening of inhibitors of melanin biosynthesis from microbial secondary metabolites, a fungal strain MR-93 which was capable of producing high level of an inhibitor was selected from plant leaf. Based on taxonomic studies, the fungus could be classified as a strain of Trichoderma sp.. The active compound (MR-93D) was purified from the culture broth by Diaion HP-20 column chromatography, ethylacetate extraction, Sephadex LH-20 column chromatography and HPLC. The inhibitor was identified as 4-hydroxy-8-isocyano-1-oxaspiro[4·4]cyclonon-8-en-2-one by spectroscopic methods of UV, ^1H-NMR, ESIMS and IR. MR-93D showed a strong tyrosinase inhibitory activity with 0.03 ㎍/㎖ of IC_50 value. It also inhibited melanin biosynthesis with 35 mm inhibition zone at 30 ㎍/paper disc in Streptomyces bikiniensis, a bacterium used as an indicator organism in this work.
정명철,전효곤,이호재,고영희,Chung, Myung-Chul,Chun, Hyo-Kon,Lee, Ho-Jae,Kho, Yung-Hee 한국미생물 · 생명공학회 1994 한국미생물·생명공학회지 Vol.22 No.5
The strain SL-387 which produces new inhibitors of aminopeptidase M, MR-387A and B, was isolated from a soil sample. The strain has branched substrate mycelia, from which aerial hyphae develop in the form of open spirals. Spore surface is smooth. Melanoid and soluble pigme- nts were observed. The isolate contains LL-diaminopimelic acid in its cell wall hydrolysate, and has no pectinolytic activity. The strain SL-387 is closely related to Streptomyces griseoruber and S. naganishii, but is different from these strains in some cultural and physiological characteristics. This strain was, therefore, designated as Streptomyces sp. SL-387. The effects of several carbon and nitrogen sources on the production of the inhibitor were examined. Among them, glucose, galactose, mannose, and xylose were effective as a carbon source and soybean meal, soytone, fish meal, and gluten meal were effective as a nitrogen source. The maximum peak of the inhibitor production in jar fermentor was obtained on the fifth day of culture.
식물추출물의 Matrix Metalloproteinase-2에 대한 저해활성
이상명,전효곤,이호재,이충환,김진희,고영희 한국생약학회 2002 생약학회지 Vol.33 No.2
The inhibitory effects of methanol extracts of 165 medicinal herbs on matrix metalloproteinase-2 (MMP-2) activity were investigated. Amongst the herbal extracts, Youngia denticulate, Flatycarya stmbilacea, Sedum kamtschaticum, Euscaphis japonica, and Corpus controversa showed a significant inhibitory effect with above 80% at 1 ㎍/ml. Above mentioned five land extracts, which exclude polyphenols and/or tannin by techniques for their removal, showed a mild inhibitory effect on MMP-2 activity except for Y. denticulate at the same concentration.
무릇에서 분리한 nortriterpenoid glycoside의 암세포에 대한 세포독성 및 함량 분석
이상명,전효곤,이충환,이호재,강진정,맹학영,고영희,Lee, Sang-Myung,Chun, Hyo-Kon,Lee, Choong-Hwan,Lee, Ho-Jae,Kang, Shin-Jyung,Maeng, Hack-Young,Kho, Yung-Hee 한국생약학회 2001 생약학회지 Vol.32 No.3
Scillascilloside E-3 (1) and E-1 (2) were isolated from the bulbs of Scilla scilloides. The cytotoxic activities of these compounds were tested against murine (B16/F-10, 3LL) and human cancer cell lines (MCF7, PC-3, HT29, LOX-IMVI, A549 and HT1080). These compounds exhibited a significant cytotoxic activities against all tested cancer cells. Futhermore, the contents of 1 and 2 in S. scilloides are 43.2 and 27.9 mg/kg, respectively.