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Development of Surface Plasmon Resonance Immunosensor for the Determination of Methyl Parathion
田中 真,由美,阪本 一平,中嶋 秀,宗 伸明,中野 幸二,CHUNG, Duck-Hwa,今任 稔彦 The Japan Society for Analytical Chemistry 2007 分析化學 Vol.56 No.9
<P>A surface plasmon resonance (SPR) sensor for the determination of methyl parathion (MP) was developed using an indirect competitive immunoassay. A sensor chip was prepared by immobilizing a MP-BSA conjugate on a gold film on the sensor chip, followed by blocking with BSA to prevent the non-specific adsorption of MP and an anti-MP antibody onto the unmodified surface of the sensor chip. An evaluation of the adsorption equilibrium of MP-BSA onto the sensor chip by measuring the SPR angle shift revealed that the adsorption equilibrium was expressed by a Langmuir-type adsorption isotherm equation. The adsorption constant and the adsorbed amount of MP-BSA onto the sensor chip were found to be 5.0 × 10<SUP>5</SUP> M<SUP>−1</SUP> and 0.95 ng/mm<SUP>2</SUP>, respectively. An MP solution (1∼5000 ppb) containing 60 ppm anti-MP antibody was introduced into the SPR sensor chip, and then the SPR angle shift was measured. The detection limit, defined as the angle shift of 85% for the blank, was found to be 10 ppb. The anti-MP antibody bound to the MP-BSA conjugate on the sensor chip was able to be dissociated by introducing an HCl-glycine solution (pH 2) into the sensor chip. Duplicate measurements with a single sensor chip were possible for at least 20 times.</P>
전중함(J. H. Chun),이현우(H. W. Lee) 전력전자학회 2006 전력전자학술대회 논문집 Vol.- No.-
In this paper discusses winding methode of single phase AC-DC reversible power converter The reversible power converter driven by multi Tap winding at both side switching control. It has a advantage that simple drive of main switching device. and obtain load current of good quality without filter circuit and free from noise or isolation for lower switching frequency. In this research, study on current type converter and inverter circuit that consist for possibility of AC-DC/DC-AC multi-level reversible converter.