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아연 결핍이 구강 점막상피조직에 미치는 영향에 관한 연구
장철성 대한구강해부학회 1977 대한구강해부학회지 Vol.1 No.1
The author observed the effect of the zinc-deficient diet (Alvares and Meuer's prescription) on the oral mucosal epithelium of the Weanling rats. The zinc deficient diets were supplied to the rats for 4 weeks. The histochemical techniques employed were alkaline phosphatase reaction, PAS reaction, Feulgen reaction, toluidine blue stain for RNA and Pearse's method for -SH and -SS groups. The results were as follows: DNA were predominantly stained in the basal cell layer. RNA were diffuseU stained in the . all layers of epithelium. Basal cell and prickle cell layer shoves intense metachromasia. Those figures were distinct in the buccal mucosa ani gingiva. PAS positive substances were increased in the each ,layer. Diffuse acid phosphatase reaction was shown in the all layers of epithelium. The reaction for -SH radicals was similar with in the control group. but the reaction for -SS radical was more intense than in the control group.
장철성,어요람 강원대학교 농업생명과학연구원 2023 강원 농업생명환경연구 Vol.35 No.1
Rubus coreanus and Rubus occidentalis of the Rosaceae family are deciduous shrubs, which are commonly used in various health functional foods. The scientific name and origin of R. coreanus and R. occidentalis are distinct. However, both plants are often confused due to the morphological similarity of their fruits and misuse of the common name of R. occidentalis, “Bokbunja,” in Korea. In the present study, molecular markers were developed using quantitative real-time polymerase chain reaction (PCR) to differentiate between the two species. Species-specific primers were designed using the internal transcribed spacer region of ribosomal DNA and chloroplast genes, including matK, atpA, rpoB, and rpoC2. When the six designed primer sets were verified, the slope ranged from -3.18 to -3.55 and the correlation coefficient exceeded 0.99. Accordingly, the efficiency ranged between 91.10% and 106.27%. Furthermore, the developed markers were verified using a specificity test on 14 other species, including the target species. The developed real-time PCR-based molecular markers are expected to prevent the misuse of R. coreanus and R. occidentalis, ultimately contributing to the protection of consumer rights in the medical herb market.
장철성,황선구,김진혁 한국유전학회 2016 Genes & Genomics Vol.38 No.8
Flowering time is an important trait associated with crop yield and quality. In a previous report, genome variation of significant DNA polymorphism was observed in the early maturing type rice mutant (EMT1) compared to that of wild type (WT). To identify quantitative trait loci (QTL) for flowering time, we constructed a linkage map of 36 InDel- and 6 SNP-markers with F2 plants derived from the cross ‘‘WT 9 EMT1’’. One potential major QTL region was detected on chromosome 6. One gene of the QTL region, Heading date 1 (Hd1), exhibited relatively high nonsynonymous substitutions in the QTL region. To evaluate reliable allele segregation related to expected Mendelian ratio, M6-3 marker developed in the Hd1 gene exhibited clear monogenic segregation (1:2:1) in heterozygous F3 plants. Both GI and Hd1 were highly induced at 56 days after sowing (DAS) in EMT1 and at 70 DAS in WT. The expression of Hd3a was significantly induced at 63 DAS, when Hd1 transcripts were slightly decreased compared with those at 56 DAS, while Hd1 was induced up to 84 DAS. These findings suggested that in EMT1, Hd1 could not suppress Hd3a under LD conditions, while OsMADS50 and LFY, which are involved in temperature responsive pathways, were significantly induced at 56 DAS, 14 days earlier than in WT.