Fusobacterium nucleatum에 의해 생성되는 황화합물 검출 방법의 비교

임회순,정만태,김병국,김재형,정성수,오종석,강미선 대한안면통증∙구강내과학회 2003 Journal of Oral Medicine and Pain Vol.28 No.2

Fusobacterium(F.) nucleatum, one of the bacteria causing halitosis, produces the ,volatile sulfur compounds(VSC) such as H2S in the media containing sulfur components, and forming FeS by binding with iron components in broth. The amount of sulfur compounds produced by F. nucleatum was measured to assess the usefulness of method using spectrophotometer. After F. nucleatum was incubated in the media containing ZnCl2 and chlorhexidine dihydrochloride the concentration of sulfur compounds measured by Halimeter was compared with the optical density measured by spectrophotometer. 1. When F. nucleatum was cultured in the closed tube for 24 hours, the concentration of VSC was increased to above 20,000 ppb measured with Halimeter, and the optical density(O.D.) was 0.429±0.040. When being cultured in the open tube, the concentration of VSC was slightly increased to 600±10 ppb, and O.D. to 0.131±0.053. 2. When pH of the media cultured with F. nucleatum for 24 hours was adjusted and incubated for 24 hours, O.D. was about 1.000 in media adjusted to pH 7.0 or 6.5, 0.704 in media adjusted to pH 6.0, 0.295 in media adjusted to pH 5.5, -0.016 in media adjusted to pH 5.0, and -0.119 in media adjusted to pH 4.5. 3. The concentration of VSC, O.D. were above 20,000 ppb, 2.064±0.007 by the addition of 0.03125% ZnCl2 in media, 157±13 ppb, 0.329±0.052 by the addition of 0.25% ZnCl2. The concentration of VSC and O.D. were 5,887±2823 ppb and 1.290±0.010 by the addition of 0.0003125% chlorhexidine dihydrochloride, 893±247 ppb and -0.452±0.106 by the addition of 0.02% chlorhexidine dihydrochloride. These results suggested that the sulfur compounds produced by F. nucleatum was similarly quantified by HalimeterⓇ and spectrophotometer, so the measurement of optical density by spectrophotometer was useful in the study of halitosis.

일부 대학생의 구강보건인식 및 행동과 치주가료필요지수에 관한 조사

조은미(Eun Mi Jo),임회순(Hoi Soon Lim),임선아(Sun A Lim) 조선대학교 구강생물학연구소 2001 Oral Biology Research (Oral Biol Res) Vol.25 No.1

.

꾸지뽕나무(Cudrania tricuspidata) 잎의 추출물이 대장암세포주에 미치는 영향

김미숙,김인애,고영종,정진안,김지은,송병준,임원봉,임회순,박지일,김서연,최홍란,김옥준 대한구강악안면병리학회 2009 대한구강악안면병리학회지 Vol.33 No.1

The phytochemicals of many plants suggest their potential use as dietary supplements in cancer chemoprevention and chemotherapy. In the present study, antitumor activity of Cudrania tricuspidata, a plant native to East Asia, was investigated. Cell growth inhibition of the extract on HT-29 colorectal adenocarcinoma using MTT colorimetric assay was determined. Apoptosis on HT-29 cells was performed by DNA fragmentation analysis. PGE2 release was measured by enzyme immunoassay, because PGE2 is a key protumorigenic prostanoid in many human cancers. For the ROS scavenging activity, ROS level was detected by laser scanning confocal microscope. It was found that methanol extract of leaves inhibits cell viability by inducing apoptosis as evidenced by DNA fragmentation. Stem bark decreases synthesis of PGE2, inflammatory mediator. Fruits exhibited pronounced ROS scavenging activity. Taken together, these results suggest that Cudrania tricuspidata exerts growth inhibition and anti-oxidation on HT-29 cells through apoptosis, ROS scavenging respectively that it may have anti-cancer properties.

PMA 처리한 인간 불멸화 상피세포에서 청색 및 적색 광 조사에 따른 활성산소 제거에 대한 비교 연구

한세우,고영종,정진안,김지은,김인애,임원봉,임회순,박지일,김미숙,김서연,김옥준,최홍란 대한구강악안면병리학회 2009 대한구강악안면병리학회지 Vol.33 No.1

Intracellular reactive oxygen species(ROS) produced in a various pathologic state was known to intermediate many cellular response such as inflammation. Recently, low level light irradiation by HeNe laser used in many clinical field could improve inflammatory state by scavenging intracellular ROS through photo-detachment/dissociation process. The purpose of this study is to investigate the differential effects of blue and red light irradiation on ROS scavenging effects. Immortalized human oral keratinocyte HaCat cells were used. Phorbol 12-myristate 13-acetate(PMA) was treated for inflammation. Red(635nm) and blue(470nm) light irradiation was carried out. To asses the intracellular ROS by light irradiation, confocal microscopic and flow cytometric assay with DCF fluorescence for total ROS and ESR spectrometry of DMPO-O2 - for superoxide anion were caried out. And microarray was performed for mRNA expression level. Released intracellular total ROS in PMA treated HaCat cell lines was dissociated efficiently by red light irradiation, while blue light irradiation did not. Rather, blue light irradiation increased ROS formation. For superoxide anion generated the first synthetic form of ROS, red light irradiation reduced its amount but blue light irradiation did not. In the mRNA expression in line with cyclooxygenase(COX) pathway, prostagrandin endoperoxide synthase 1(PTGS 1), prostagrandin endoperoxide synthase 2(PTGS 2) and phospholipase A2(PLA2) were increased by both light irradiation and they were decreased as time flows. And genes associated with ROS releasing, mRNA expressions of tumor necrosis factor receptor (TNFR) and interleukin 1beta(IL1B) were increased by 1 hour red light irradiation but did not by blue light irradiation. As a result, red and blue light irradiation showed different response in affecting the level of ROS. These findings indicate that red light rather than blue light is more useful for anti-inflammation in clinical field

광주광역시에 거주하는 고령자의 타액 내 병원성 칸디다종의 동정에 관한 연구

고영종,임원봉,김지선,김인애,권혁일,김옥수,김병국,김선미,윤숙자,강병철,임회순,김미숙,김옥준,최홍란 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.2

Candida albicans and their associated Candida species are opportunistic pathogens which exists as normal flora in the oral cavities of healthy individuals. In response to physiological changes in the host, these yeasts can become pathogenic, resulting in oral candidiasis. The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidiasis. The presently available culture and biochemical methods for detection and species identification of Candida are time-consuming and lack the required sensitivity and specificity. In this study, we have established a seminested PCR (snPCR) using universal and species-specific primers for detection of Candida species in saliva. The universal outer primers amplified the 3end of 5.8S ribosomal DNA (rDNA) and the 5end of 28S rDNA, including the internally transcribed spacer 2 (ITS2), generating 350- to 410-bp fragments from the four commonly encountered Candida spp., viz., C. albicans, C. tropicalis, C. glabrata, and C. parapsilosis. The saliva from 331 healthy and, over 50 years of aged people lived in Dong-gu, Gwangu city, was collected. Total DNA were extracted by Hoffman-Winston yeast total DNA prep. method and performed t he s nP CR. R esults appeared to b e negative on 292 people ( 88.2%), however, 2 6 people ( 7.9%) were p ositive Candida albicans, 6 people (1.8%) were positive Candida glabrata, 5 people (1.5%) were positive Candida tropicalis, and only 2 person (0.6%) were positive Candida parapsilosis. These result showed that detection and identification of Candida species could be established by saliva analysis, so that snPCR on saliva is useful method of diagnosis of clinical fields

일부 대학생의 구강보건인식 및 행동과 치주가료필요지수에 관한 조사

조은미,임회순,임선아 朝鮮大學校 口腔生物學硏究所 2001 Oral Biology Research (Oral Biol Res) Vol.25 No.1

Dental caries and periodontal disease are the most common oral disease. Periodontal disease repidly increases during adolescence and the main cause of tooth loss in adults. This study was performed to investigate the oral health behavior and periodontal status using the community periodontal index of treatment needs(CPITN) and to find the associated factors with it of university students. The subjects were 615 students who attended to periodic health examination which was held from May 30th to June 2nd, 2000. The results were as follows; 1. The subjects were composed of 64.5% in males and 35.5% in females. The types of oral health practice were tongue brushing(61.8%). use of mouth rinsing solution(19.8), abstention of sweet foods(17.1%) in order. 2. The treatment of dental caries, scaling, tooth extraction and dental prosthesis were main causes of visit to dental clinic. 3. The scores for knowledge of oral health(male : 6.53, female : 6.70)and the number of dental caries(male : 1.96, female :2.27)were not statistically significant. 4. The degree of CPITN were 20.9% and 31.7% for 0, 3.0% and 5.5% for I, 63.7% and 55.9% for 2, 11.1% and 6.9% for 3 and 0% for 4 in males and females. 5 In males, the assorciated factors with peridonotal status were effective and frequent tooth brushing, self-congnition of oral health status, participation of oral health education and amount of dental caries. In females, efective tooth brushing, and scaling were associated with periodontal status. As a result of this study, it was concluded that more than 70 percent of subjects needed periodontal care. To keep good oral health for a lifetime. we should understand the necessity of appropriate oral health education and the early treatment of periodonal disease during adolescence.

Vitamin D Promotes Odontogenic Differentiation of Human Dental Pulp Cells via ERK Activation

우수미,임회순,정경이,김선미,김원재,정지연 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.7

The active metabolite of vitamin D such as 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) is a well-known key regulatory factor in bone metabolism. However, little is known about the potential of vitamin D as an odontogenic inducer in human dental pulp cells (HDPCs) in vitro. The purpose of this study was to evaluate the effect of vitamin D3 metabolite, 1α,25(OH)2D3, on odontoblastic differentiation in HDPCs. HDPCs extracted from maxillary supernumerary incisors and third molars were directly cultured with 1α,25(OH)2D3 in the absence of differentiation-inducing factors. Treatment of HDPCs with 1α,25(OH)2D3 at a concentration of 10 nM or 100 nM significantly upregulated the expression of dentin sialophosphoprotein (DSPP) and dentin matrix protein1 (DMP1), the odontogenesis-related genes. Also, 1α,25(OH)2D3 enhanced the alkaline phosphatase (ALP) activity and mineralization in HDPCs. In addition, 1α,25(OH)2D3 induced activation of extracellular signal-regulated kinases (ERKs), whereas the ERK inhibitor U0126 ameliorated the upregulation of DSPP and DMP1 and reduced the mineralization enhanced by 1α,25(OH)2D3. These results demonstrated that 1α,25(OH)2D3 promoted odontoblastic differentiation of HDPCs via modulating ERK activation.

Quantitative Analysis of Weissella cibaria against Periodontopathic Bacteria by Real-time PCR

강미선,임회순,김선미,이현철,오종석,임유진 대한미생물학회 2009 Journal of Bacteriology and Virology Vol.39 No.4

The objective of this study was to analyze quantitatively whether Weissella cibaria could affect the proliferation offive periodontopathic bacteria, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Aggregatibacteractinomycetemcomitans, and Fusobacterium nucleatum, after incubation for 8~48 h. In addition, by using real-timePCR with a dual-labeled probe, each growth of bacteria was examined under different growth media conditions. Theproliferation of periodontopathic bacteria was significantly inhibited by W. cibaria after incubation for 24~48 h (p <0.05), whereas the growth of W. cibaria was not affected by these pathogenic bacteria. The growth of P. gingivalis, T. forsythia and T. denticola significantly increased in each growth media after incubation for 24 h (p < 0.05), as comparedto the culture in mixed growth media. However, no differences in the growth of five periodontopathic bacteria wereobserved between each growth media and mixed media after incubation for 48 h. The growth and pH of W. cibariaculture significantly were changed in MRS after incubation for 24~48 h (p < 0.05), as compared to the bacterial culturein mixed growth media. The pH of P. gingivalis and F. nucleatum culture significantly was changed in both growthmedia and mixed media after incubation for 24~48 h (p < 0.05). Our data indicate that W. cibaria significantly inhibitsthe proliferation of five periodontopathic bacteria and each growth of bacteria is quantitatively analyzed under variousmedia conditions by real-time PCR.

젊은 성인에서 심상유두개수와 전기미각역치의 상관성

한동훈,박지일,임회순,김재형,Han, Dong-Hoon,Park, Ji-Il,Lim, Hoi-Soon,Kim, Jae-Hyung 대한안면통증구강내과학회 2011 Journal of Oral Medicine and Pain Vol.36 No.1

The aims of this study were to establish the effect of gender and age on number of fungiform papillae and electrogustometric thresholds of the tongue, and to determine the relation between number of fungiform papillae and electrogustometric threshold. Sixty-two healthy subjects of 20 to 39 years old were enrolled in this study. Examination areas were defined as center, anterior, left lateral, right lateral, and posterior on the dorsal surface of the tongue, which was stained with methylene blue dye and photographed with a scale bar on it. The number of fungiform papillae within $1\; cm^2$ square area of each examination site was counted twice. The counted values were averaged to produce means. Taste thresholds were measured three times on each examination area using an electrogustometer. Means of the threshold values were calculated. 1. The number of fungiform papillae was the largest at the anterior examination area, followed by right lateral, left lateral, posterior and central. Women had more fungiform papillae than men at anterior, right lateral, and center areas. Subjects in twenties showed more fungiform papillae than those in thirties at anterior, right lateral, and left lateral areas. 2. Anterior area of the tongue measured the lowest electrogustometric threshold, followed by left lateral, right lateral, posterior and center. The threshold was higher in men at anterior and right lateral areas. The subjects in thirties had higher threshold only at right lateral area than those in twenties. 3. Electrogustometric threshold was inversely correlated with the number of fungiform papillae at anterior and posterior areas. The results showed that the number of fungiform papillae and taste threshold varied according to tongue locus, and they were influenced by age and gender. The results also suggested that the number of fungiform papillae was related to electrogustometric threshold inversely.

구강작열감 증후군에 대한 논문 고찰

최성현,이빈나,임회순,오원만,김재형 대한턱관절교합학회 2019 구강회복응용과학지 Vol.35 No.3

Burning mouth syndrome (BMS) is defined as the xerostomia, burning sensation and various discomfort of tongue and oral mucosa. BMS can occur in both men and women, but is more frequent in middle-aged menopausal women. Because exact cause can’t be identified clearly and it is hard to make diagnosis in clinic, the purpose of the treatment have been to relieve symptoms. Etiology of BMS is divided into local, systemic, and psychological factors. α-lipoic acid, clonazepam, supplemental therapy and cognitive behavior therapy can be prescribed for BMS. Nowdays, many experts focus attention on effect of combination therapy. It is necessary to solve the symptoms of the patients by combination of pharmacological approach and psychotherapy with cognitive behavior therapy considering the factors in various aspects.