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임현섭,한창수,황순웅,신규식 제어·로봇·시스템학회 2012 International Journal of Control, Automation, and Vol.10 No.2
This paper presents the procedure and results of the multi-objective design optimization of a seven-degrees-of-freedom (7DOF) robot manipulator for better global performance, which pertains to the Global Conditioning Index (GCI) and the Structural Length Index (SLI). The concepts of, and the calculation techniques for, GCI and SLI are introduced to allow their use as objective functions for op-timization. The optimization techniques, which are Sequential Two-point Diagonal Quadratic Ap-proximate Optimization (STDQAO), the Progressive Quadratic Response Surface Method (PQRSM), the micro genetic algorithm (μGA), and the evolutionary algorithm (EA), were explained briefly, and they are being used to optimize the global performance indices of the robot manipulator. Also, the re-sults of the optimization and comparison of the four optimization methods are summarized in tables.
임현섭,이미연,문제선,문중경,유용만,조인숙,배한홍,Matt deBoer,주호종,John Hammond,Andrew O. Jackson 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.1
Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed:Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treatments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed:Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.