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임다정,박준철,장시훈,최준,박노형 한국섬유공학회 2021 Fibers and polymers Vol.22 No.3
Numerous studies have addressed the utilization of polyimide (PI) and silica for improving the properties of hybridcomposites. However, the applicability of polyhedral oligomeric silsesquioxanes (POSSs) has not been elucidated. Herein,inorganic nanoparticles containing poly(amic acid)s (PAAs) were synthesized; further, glass fibers (GFs) were impregnatedwith PAA solutions to obtain PAA prepregs. PI-based fiber-reinforced composites were manufactured with the prepregs byusing the hand lay-up technique. The morphology and the thermal, mechanical, and dielectric properties of the compositeswith inorganic nanoparticles were analyzed to investigate the effects of inorganic additives. The addition of inorganicparticles were observed to primarily improve the properties of the PI-based fiber-reinforced composites as compared to thoseof the neat PI composites. While this improvement was limited in the case of the addition of POSSs owing to the aggregationand a poor interaction with the PI matrix, silica-containing composites exhibited superior thermal, mechanical, andparticularly dielectric properties as compared to those of the composites containing POSSs; this was because of the increasein the dispersion of silica nanoparticles that was caused by a silane coupling agent (SCA). The results obtained in this studycan be utilized for applications pertaining to the design of PI composites with high mechanical strengths, enhanced thermalstabilities, and low dielectric constants.
Global trends in regulatory frameworks for animal genome editing in agriculture
임다정,최인철 사단법인 한국동물생명공학회 2023 Journal of Animal Reproduction and Biotechnology Vol.38 No.4
Revolutionary advancements, such as the reduction in DNA sequencing costs and genome editing, have transformed biotechnology, fostering progress in manipulating biomolecules, engineering cells, and computational biology. Agriculture and food production have significantly benefited from tools like high-throughput microarrays, accelerating the selection of desired traits. Genetic engineering, especially utilizing genome editing, facilitates precise alterations in plants and animals, harnessing microbiomes and fostering lab-grown meat production to alleviate environmental pressures. The emergence of new biotechnologies, notably genome editing, underscores the necessity for regulatory frameworks governing LM (living modified) organisms. Global regulations overseeing genetically engineered or genome-edited (GE) organisms, encompassing animals, exhibit considerable diversity. Nonetheless, prevailing international regulatory trends typically exclude genomeedited plants and animals, employing novel biotechnological techniques, from GMO/ LMO classification if they lack foreign genes and originate through natural mutations or traditional breeding programs. This comprehensive review scrutinizes ongoing risk and safety assessment cases, such as genome-edited beef cattle and fish in the USA and Japan. Furthermore, it investigates the limitations of existing regulations related to genome editing in Korea and evaluates newly proposed legislation, offering insights into the future trajectory of regulatory frameworks.
임다정,양시영,노미영,이철원,김진철,김인선 한국곤충학회 2017 Entomological Research Vol.47 No.6
Myzus persicae is an important insect pest that reduces crop production worldwide. The use of pesticides for aphid control has generated much concern related to insect resistance and undesirable environmental effects. In an effort to discover new alternatives to counter M. persicae, we found that Pseudomonas isolate DJ15 produced insecticidal metabolites. To isolate the insecticidal metabolites, a cell‐free supernatant of DJ15 was extracted and subjected to bioassay‐guided chromatography. Based on the structures elucidated in instrumental analyses, the metabolites were identified as xantholysins A and B. The metabolites showed strong insecticidal activity against M. persicae with 50% mortality at levels of 13.4 and 24.6 μg/mL for xantholysins A and B, respectively. This is the first study to identify xantholysins as insecticidal metabolites against M. persicae.
한우와 칡소의 선발신호 분석을 통한 한우의 인위적 선발 영역 탐색
임다정,이승환,최봉환,채한화,손주환,유다영,김태헌,강희설,조용민 경상대학교 농업생명과학연구원 2013 농업생명과학연구 Vol.47 No.6
Korean cattle (Hanwoo) are categorized into three types of breeds according to their color, brown, brindle and black. Among the three breeds, the brown Hanwoo has been subjected to intensive artificial selection over the past seventy years to improve meat production traits such as carcass weight and marbling. In this study, three methods (Rsb, iHS and FST) were applied to identify genomic regions of artificial selection for traits in Hanwoo. To identify traces driven by the artificial selection process called the Hanwoo breeding program, we scanned the genome of set of brown and brindle Hanwoo using 50K SNP Chip. As a result, we identified 41, 23 and 50 highly homozygous regions that seem to be very strong and/or recent artificial selections in brown Hanwoo compared to the brindle Hanwoo from Rsb, iHS and FST, respectively. To go further in the interpretation of the observed signatures of selection, we subsequently performed functional enrichment analysis using candidate genes. As a result, candidate genes have enriched GO terms such as muscle development or immune response. Artificial selection might have targeted most of these genes was mainly oriented towards improvement of meat production in brown Hanwoo. 우리나라 고유 품종인 한우는 표현형 중 외모에 따라 일반적으로 한우라 불리는 황갈색 한우, 칡소, 흑우로 구분되어 있다. 한우 집단 중, 황갈색 한우만이 육질, 육량 형질을 중심으로 체계적인 개량프로그램에 의해 개량되었다. 본 연구에서는 황갈색 한우와 칡소의 50K 고밀도 칩을 활용하여 선발신호를 검출할 수 있는 세 가지 분석법(Rsb, iHS, FST)에 따라 인위적 선발에 의해 진화적으로 선택된 유전체 영역을 탐색하였다. 그 결과, Rsb, iHS, FST 분석법에 따라 각각 41개, 23개, 50개의 후보유전체 영역이 칡소와 비교하였을 때 황갈색 한우에서 유의적으로 다름을 알 수 있었다. 또한, 후보 유전체 영역 내에 존재하는 유전자군을 대상으로 유전자 기능에 대한 주석 달기를 진행하였을 때, 근육 발달, 면역 반응 등의 기능들이 통계적으로 유의함을 알 수 있었다. 이러한 유전체 영역들이 진화적 관점에서 황갈색 한우의 적응과정에 있어서 육질 등 생산성을 높이는 데에 기여하였다고 사료된다.