만성 B형 간염 환자의 파라핀 포매 간 조직에서 중합효소 연쇄반응법에 의한 B형 간염 바이러스의 검출에 관한 연구

이혜수,오강렬,김주헌,김윤정,최삼임,이동근,김상호,이혜수,최삼임 대한병리학회 1996 Journal of Pathology and Translational Medicine Vol.30 No.6

胃癌 組織의 Lysozyme, Carcinoembryonic Antigen 및 Keratin에 對한 免疫 組織 化學的 硏究

이혜수,허장호,최호열 의과학연구소 1991 全北醫大論文集 Vol.15 No.1

Gastric cancer is the most common tumor in Korean. To investigate the significance of the immunohistochemical study using a immunoperoxidase technics on the formalin fixed, paraffin embedded section of the gastric cancer tissues, peroxidase-antiperoxidase stain for lyso- zyme, carcinoemvbryonic antigen(CEA) and keratin were carried out on the 68 cases of the gastric cancer of 27 cases of well differentiated adenocarcinoma, 14 cases of moderate differentiated adenocarcinoma, 22 cases of poorly differentated adenocarcinoma and 5 cases of the signer ring cell carcinoma, and 21 cases of the no- rmal gastric tissues. The results were as follows. 1. Rate of the positivity of the lysozyme on the gastric cancer was 69.1%(47/68). And rate and degree of the positivity was markedly decreased in the poorty differentiated adenocarcinoma compared with well differe- ntiated adenocarcinoma. 2. In the stain for the carcinoembryonic antigen, the rate of positivity was 83%(59/68), and moderate to st- rong positive reactions were shown independent of its differentiation. 3. Keratin in the normal and all gastric cancer tissues was not demonstrated. Above results suggest that detection of the lysozyme and carcinoembryonic antigen using a peroxidase-anti- peroxidase method are helpful to the diagnosis of the degree of differentiation of gastric cancer tissues.

정규직 전환이 아니면 그만두어야 하나요?

이혜수 한국비정규노동센터 2003 비정규 노동 Vol.2003 No.10

人體 子宮頸部癌 組織의 Keratin, Herpes Simplex Virus Type 2, Carcinoembryonic Antigen 및 Lysozyme에 對한 免疫 組織化學的 硏究

이혜수,조성남,최삼임,최호열 의과학연구소 1991 全北醫大論文集 Vol.15 No.1

The most common malignant meoplasm in the female genital tract is uterine cervical carcinoma. To investigate the significance of the immunoperoxidase staining method on the histopathological differential diagonosis of the uterine cervical cancer, the author performed the immunoperoxidase stain for the keratin, herpes simplex virus type Ⅱ(HSV-Ⅱ) snyihrn, vstvinormntyonic antigen(CEA) and lysozyme in the normal(20cases),squamous metaplasia (15 cases), dysplasia(23 cases), carcinoma in situ(4 cases) and invasive squamous cell carcinoma(28 cases) of the uterine cervix tissue using a peroxidase-antiperoxidase method. The result obtained were as follows. 1Keratin in the normal uterine cervical squamous epithelial tissues was strongly positive reaction, but in the mild to moderate dysplasia, keratin was moderately stained, and in the severe dysplasia, carcinoma in sity, and large cell type of invasive carcinoma, the keratin posotivity ws markedly decreased, and in the small cell type, keratin ws not stained. 2.Herpes simplex virus type Ⅱ was moderate to strongly stained in the large cell, keratinizing and nonkeratinizing types as compared with normal uterine cervical squamous epithelium, but in small cell type, negative or only only trace positive reaciton was shown. 3. CEA in severd dysplasia, carcinoma in situ, and large cell, keratinizing and nonkeratinizing type of squamous cell carcinoma tissus was stained with moderately to strongly positive but small cell type showed negative or only trace positivity for CEA. 4. Lysozyme was not demonstrated in normal and all tumor cells. These results suggest that immunohistochemical stain for keratin, HSV-Ⅱ, and CEA using a peroxidase-an-tiperoxidase(PAP) methods are helpful to the diagnosis and classification of ht uterine cervical carcinoma.

Mepirizole 誘導 十二指腸潰瘍의 發生機轉 및 治療過程에 關한 實驗的 硏究

이혜수,윤윤호,정태섭,박승창,최호열,김상호 의과학연구소 1993 全北醫大論文集 Vol.17 No.2

To Investigate the pathogenetic mechanism of the mepirizole-induced duodenal ulcers, measurement of the acidity and amount of the gastric juice, comparison of the gross and histopathologic findings of the duodenal ulcers caused by oral, subcutaneous, intraperitoneal, and intramuscular administration of the mepirizole(200mg/kg), and observation of the effects of the truncal vagotomy to mepirizole-induced duodenal ulcers were performed. And their healing processed were observed. The results were as follows : 1. In group of oral administration of mepirizole, the gastric acidity was increased, but the amount of gastric juice was decreased with the passage of time in comparison with normal control group. 2. The duodenal ulcers were produced in all methods of mepirizole administration, especially in oral and subcutaneous administration groups, incidence rates were 90 and 100%, ulcer indices were 21.1+4.6 mm2 and 33.6+6.3em2, but duodenal perforation rates were 20% or less and there was no death case regardless of methods of administration. 3. After vagotcmy, duodenal ulceration by mepirizole was significantly inhibited in both short(1, 4 weeks) and long (1 year) time passed groups. 4. The healing of the mepirizole-induced duodenal ulcers were rapidly accomplished in 15 days for the most parts under the gross and microscopic observation. From the above suggest that production of duodenal ulceration by mepirizole is the consquence of exposure of the duodenal mucosa to the action of gastric acid that overwhelm the normal defenses and their healing is rapidly accomplished.

DMBA(9,10-Dimethyl-1,2-Benzanthracene) 誘導 惡性 纖維組織球腫에 關한 實驗的 硏究(Ⅱ)

이혜수,김형겸,김달식,최삼임,최호열,김상호 의과학연구소 1997 全北醫大論文集 Vol.21 No.1

The author has studied the morphologic characteristics and hitogenesis of potent carcinogen DMBA(9,10-dimethyl-1,2-benzanthrancene) induced tumors in knee joint of Wistar rats which were injected intraarticularly with 0.1ml of 2% DMVA paraffin solution(group A : 2 times, group B : 3 times, 4 weeks interval). The results were obtained as follows : 1. Induced tumors all developed single and had similar growing pattern, but they were different in incidence, developing period, and tumor size to frequency of injection. Internal organs to be metastased were lung, kidney, andliver in order, and most of the primay tumors were adhered to adjacent bone and joint. These findings were also revealed radioligically. 2. Grossly, most of the tumors well encapsulated, hard, nodular mass which cut surface disclosed whitish yellow to bright yellow fibrous tissue, and partially necrotic, hemorrhagic, and cystic. 3. Microscopically, the findings were compatible with typical forms of mailgnant fibrous histiocytoma(MFH), storiform-pleomorphic type. 4. Hisothemically, most of the tumor cells were PAS positive, and abundant collagen and reticulin fibers stained with trichrome, reticulin and van Gieson, and myofibroblasts confirmed by trichrome stain. 4. Enzyme histochemically, most of the tumor cells disclosed strong acid phosphatase activity, and weak nonspecific esterase activity, and weak activity for nomoclonal antibody to the rat peritoneal macrophage TRPM 2. These experimental results demonstrate that DMBA-induced tumors are typical forms of MFH, storiforme-pleomophic type and suggest that these tumors are originated from mesenchymal cells which do not belong to the monouclear phagocytic system.

脾摘出 白鼠의 網內系 貪食能에 미치는 ACTH 및 Cortisone의 影響

이혜수,김형겸,최호열 의과학연구소 1991 全北醫大論文集 Vol.15 No.4

To investigate relationship between the mechanism of ACTH(RES acclerator) and Cortisone(RES suppressor) on the reticuloendothelial phagocytic function and the role of spleen, after the intramuscular injection of ACTH(1 USP & 2 USP/100gm of body weight) and Cortisone(1mg/100gm of body weight) into the splenectomized rats, the phagocytic activity of RES was assessed by means of carbon clearance method and their effects were compared with nosplenectomized group. The results were obtained as follows : 1. In the nosplenectomized group treated with ACTH, the phagocytic activity was increased markedly, but decreased significantly in the Cortisone treated group compared with control group. 2. In the splenectomized group, the enhancing effects of ACTH and suppressing effects of Cortisone on the RES function were not observed. 3. From these results, it is suggested that the influences of ACTH and Cortisone on the phagocytic function of RES was exhibited through the mediation of spleen.

각종 상피성암조직 Lysozyme 에 대한 면역조직화학적 연구

이혜수,조수철,정신호,최호열,김상호 의과학연구소 1990 全北醫大論文集 Vol.14 No.2

To investigate the relationship between the epithelial malignant tumor and lysozyme, lysozyme distribution in cancer tissues were assayed by immunoperoxidase stain with PAP(peroxidase-antiperoxidase) method. The results were as follows: 1. In stomach cancer group, lysozyme positivity in cancer tissue was 81.8% (18/22), and degree of positivity was increased according to the grade of differentiation of the cancer tissues. 2. In colon cancer group, lysozyme positivity was 80%(4/5) and the positive degree was increased according to the differentiation grade, too. 3. In lung cancer group, tissue lysozyme positivity was only 14.3%(1/7), and degree of positivity was very weak. 4. In breast and hepatic cancer, lysozyme was not detected in cancer tissues. 5. In other epithelial cancer group, lysozyme in cancer tissues were detected although degree of positivity was weak to moderate. From the above results, detection of the lysozyme in cancer tissues be helpful to the diagnosis and estimation of prognosis of the epithelial malignant tumor.

뇌연화소의 GFA에 대한 면역세포화학적 검색

이혜수,이동근,이정융,강명재,최호열,김상호 의과학연구소 1988 全北醫大論文集 Vol.12 No.3

To investigate the origin of macrophages in the nontraumatic infarcted site of brain, time sequential (one, 2, 3, and 4 weeks) routine histologic and histochemical examinations and GFA( glial fibrillary acidic protein) detections with PAP(peroxidase-antiperoxidase) technique were performed after brain infarction was induced by injection of autologous blood clot, and following results were obtained. 1. Grossly, brain infarcts were observed mostly in the right parietal and temporal lobe and ischemic. infarcted areas of one week old appeared edematous and of two week old were necrotic and clearly demarcated with surrounding brain tissue. In the three week old infarcts, a line of demarcation between the lesion and surrounding brain tissue were more distinct and slightly contracted. And four week old infarcts were more contracted, 2. In histologic and histochemical changes, one week old infarcts were characterized by generalized edema, parenchymal degeneration and necrosis, and acute inflammatory changes with infiltration of gitter cells. Two week old infarcts showed pseudocystic changes with chronic inflammatory cell infiltrations, proliferation of pericystic cells and capillaries, and evidence of phagocytosis and mitosis in infiltrating cells. In three week old group, size of cysts were smaller, and pericystic cell number and capillary proliferations were slightly decreased, but phagocytosis and mitotic figures were persistent. In four week old group, number and size of pseudocysts were decreased and contracted, and cells of the surrounding the lesions were decreased and gliosis were noticed. 3. Although cellular composition in the infarcts were variable according to the experimental group, in all experimental group, GFA positive astrocytes and GFA negative cells were observed, and some of these cells showed mitotic figure. It is thought the GFA negative cells consists of many microglia according to their morphological features. According to these results, it is considered that macrophages in the brain infarcts originated from multiple sources, both blood monocytes and microglial cells according to the causes of infarcts and time after lesion.

태반 대식세포에 관한 세포학적 및 세포화학적 연구

이혜수,박상수,김달식,최호열,김상호 의과학연구소 1996 全北醫大論文集 Vol.20 No.2

To investigate the cytologic and cytochemical charactericstics of the human placental macrophages, histologic, histochemical and enzyme histochemical stains were performed on the 20 cases of normal fullterm placental tissues. The result obtained were as follows ; 1. Histologic examination : The macrophages in the placental villi had round or oval nuclei and were classified three types according to the cytoplasmic features. Type 1 cell was small cell with dense eosinophilic cytoplacmin margin, type 2 cell was large cell with many various sized vaculoated cytoplasm (Hofbauer cell), and type 3 cell was intermediate cell with eosinophilic cytoplasm and indistinct cytoplasmic margin. Most of the type 1 and 2 cells were noticed in edematous stroma and type 3 cells were observed in normal appearing stroma of the villi. 2. Histochemical examination : All placental macrophages including Hofbauer cells were not stained with any of histochemical stains for PAS, chromolipid and fat. 3. Enzyme histochemical examination : Hofbauer cells and other macrophages in placental vilous stroma showed moderate to strong activites to acid phosphatase and nonspecific esterase, but they were not stained for peroxidase. From these results, it is suggested that Hofbauer cells and other macrophages in placental villous stroma have characteristics of resident macrophages, and morpholgically changed according to placental condition.